Objective: to examine gender-related differences in the postresuscitative brain functional and structural changes.Materials and methods. Congenital and acquired behaviors were studied in 100 adult albino rats of both sexes in the control and after experienced 12-min cardiac arrest during 3 months (6 tests); the conditions of highly sensitive to ischemia of cerebral formations were subject to neuromorphological quantitative studies, and biochemical plasma parameters were determined.Results. Sexual differences in the integrative activity of the brain, in the state of neuronal populations in different regions of the hippocampus and cerebellum, and in plasma biochemical parameters were established in normalcy. Common and gender-related functional and structural changes of the brain and changes in plasma biochemical parameters were revealed in the postresuscitative period.Conclusion. Further studies of the gender-related mechanisms of development are required to develop specific methods for the prevention and therapy for disease.
The paper summarizes the results of the authors’ own experimental studies and the data available in the literature on the implication of the neuroendocrine system, sexual dimorphism and reproductive hormones in the pathogenesis and outcome of postresuscitative disease. There is evidence for the important role of systemic hormonal balance and particularly endogenous and exogenous sex hormones in postresuscitative rehabilitative processes.
The paper summarizes the results of the authors’ own experimental studies and the data available in the literature on the implication of the neuroendocrine system, sexual dimorphism and reproductive hormones in the pathogenesis and outcome of postresuscitative disease. There is evidence for the important role of systemic hormonal balance and particularly endogenous and exogenous sex hormones in postresuscitative rehabilitative processes.
The investigation was undertaken to study early postresuscitative sexual differences in cerebral structural and functional changes 12 min after cardiac arrest in albino rats. There were great sexual differences in orientative-investigating behavior and anxiety before, as well as neurological recovering processes after clinical death, without distinctions in cardiopul-monary resuscitative processes and postresuscitative mortality. Common changes in the content of peptide substances having a molecular weight of as high as 5 kDa in the brain tissue and gender-associated features were revealed in the early postoperative period. The baseline increased behavioral activity and a rapid postresuscitative recovery of females, unlike males, are associated with the early postresuscitative development of neurodystrophic and degenerative changes in the neuronal populations of the hippocampus and cerebellum.
The impact of 12-minute clinical death on congenital and acquired behaviors, the content of biogenic amines, the composition and size of neuronal populations in some regions of the albino rat brain were studied. It was shown that there were postresuscitative changes in emotional responsiveness and position-finding and explorative reactions and impairments in learning and memory processes, and decreases in physical endurance and algesthesia. These processes were associated with the changes in the levels of serotonin and noradrenalin in the frontal cortex of the brain, in those of dopamine and serotonin in the striatum, in some plasma biochemical parameters, and loss of neurons in the sector CA1 of the hippocampus and in the lateral sector of the cerebellum.
The impact of 12-minute clinical death on congenital and acquired behaviors, the content of biogenic amines, the composition and size of neuronal populations in some regions of the albino rat brain were studied. It was shown that there were postresuscitative changes in emotional responsiveness and position-finding and explorative reactions and impairments in learning and memory processes, and decreases in physical endurance and algesthesia. These processes were associated with the changes in the levels of serotonin and noradrenalin in the frontal cortex of the brain, in those of dopamine and serotonin in the striatum, in some plasma biochemical parameters, and loss of neurons in the sector CA1 of the hippocampus and in the lateral sector of the cerebellum.
The elevated cross-shaped labyrinth test carried out on highly, moderately, and low active experimental animals revealed significant differences in the baseline density and composition of neuroglial populations of numerous formations of the brain, as well as in the early structural and functional sequels of experienced clinical death. The most pronounced postresuscitation abnormal changes were observed in highly active animals and the least marked ones were seen in moderately active animals.
A comparative morphometric study of postresuscitation changes in the neuronal populations of the pyramidal cells from hyppocampal sector CA1 and Purkinje cells of the lateral cerebellar region in the course of postresuscitation period after 12-minute cardiac arrest in rats has shown that the changes differ in severity and pattern. In the pyramidal cells there were reversible dystrophic alterations of the neurons. Purkinje cells showed death of some neurons, this process progressed in the course of postresuscitation period. A positive effect of the peptide kyotorphin on the brain condition after resuscitation was found but its efficacy in different neuronal populations varied.
In experiments on rats we studied the effects of antioxidant and membrane-protecting agent mexidol and neuropeptides delta sleep-inducing peptide and oxytocin administered during resuscitation after 12-min clinical death. Individual and combination treatment with these substances accelerated recovery of the neurological status and partially or completely corrected behavioral disorders associated with changes in the emotional and motivational status. Combined administration of mexidol and oxytocin most significantly promoted postresuscitation recovery of functional activity in the central nervous system.
A complex of behavioral tests revealed diminished anxiety, increased locomotor and exploratory activities, and changes in different learning tests in resuscitated animals. Mexidol alone and in combination with kyotorphin exhibited antistressogenic and nootropic activities, and led to a compensation of ischemic lesions in rats. It can be stated that a the injection of mexidol in combination with kyotorphin yielded better results due to its neuroprotective effect in the CA1 and CA4 fields of the hippocampus.
