We produced the first systematic map on how commercial video games engage with biodiversity and conservation. This includes 380 papers, five languages, 450 unique games, empirical and non-empirical literature, as well as an interactive online database of the available evidence. Non-empirical work often scrutinises the extent that game design encourages or condemns exploitation of nature. Empirical papers are overwhelmingly non-experimental; research most often explores how video games represent real-world wildlife, influence players’ ecological perceptions, or inform players’ interactions with virtual nature. Despite the volume of literature, it is difficult to infer the causal effects of video games – especially on driving real-world pro-ecological action. Our map highlights several promising areas for future study, such as: consider the role of player differences on the impact of games, observe actual behaviours and how these change after play, and pursue interdisciplinary collaborations with the games industry for more thorough, applicable, and novel findings.

The Indochinese leopard (Panthera pardus delacouri) is one of the most endangered leopard subspecies, restricted to less than 4% of its historical range across Southeast Asia. With severe population declines driven by poaching, habitat loss and fragmentation, and prey depletion, effective recovery requires urgent, spatially-informed conservation action. This study presents the first comprehensive connectivity analysis for the Indochinese leopard across its extant and former range, aiming to identify core habitats, connectivity corridors, and opportunities for population restoration. We used resistant kernel modelling through the CoLa Decision Support System to evaluate landscape connectivity across 14 forest complexes in the extant range and 11 potential reintroduction areas, considering scenarios of extant, reintroduction, and combined range connectivity. Importance to connectivity was assessed based on kernel extent, movement density, and protected area overlap. Our results confirmed the Dawna–Tenasserim landscape, spanning parts of Thailand and Myanmar, and Peninsular Malaysia as the most critical strongholds to conserve. In the former range, our analysis revealed potential for large-scale metapopulation structural connectivity across Cambodia and Lao PDR, despite recent local extirpations. Realizing reintroduction opportunities will require complementary actions such as reducing poaching, restoring prey, and improving habitat management. In some complexes, intensive landscape restoration may be needed to reconnect major core areas with smaller reintroduction complexes that could still be critical for meta-population connectivity. Despite increasing human pressure in the region, substantial habitat and connectivity potential remain. Our framework provides a spatial foundation to prioritize actions for preventing extinction and guiding large carnivore recovery across fragmented tropical landscapes.

Identification of highly biodiverse areas has become a crucial step in protecting species richness, especially considering the rapid collapse of biodiversity and the limited funds available to avert, far less to reverse, these trends. Therefore, we aimed to identify the most important areas for the conservation of specified mammalian groups in Southern Asia, a region rich in biodiversity hotspots threatened by increasing rates of habitat loss and other anthropogenic activities. To achieve this, we modelled the occupancy of ungulates and of small, medium and large carnivorans at 20 study sites across the region and identified hotspots of species richness. We analysed the variation of estimated space use between different species groups and ranked areas according to their predicted importance for mammalian species conservation. Our results reveal a significant positive correlation in the spatial utilization patterns of competitive carnivores, yet no correlation among carnivores and their prey species, suggesting that anthropogenic impacts in the region are constraining species to coexist in only the few remaining suitable areas, superseding interactions between species guilds. Although the rank of site importance varied amongst species groups, we were able to identify a consensus on sites that are crucial for the conservation of all groups considered. Most of these top-ranking sites were located in the peninsular region of Thailand. We argue that, of the areas assessed, these sites represent the most important refuges for species conservation in the region, and their protection is critical for the maintenance of the biodiversity in Southern Asia.

Southeast Asia hosts more felid species than any other region and, although smaller (< 30 kg) felids have important ecological roles, regional conservation has mainly focused on a few charismatic big cats. Information on the ecology and conservation status of small felids is often lacking or geographically limited. We used empirically derived scale-optimized models for seven species in three regions of Southeast Asia (mainland, Borneo and Sumatra) to evaluate the effectiveness of the existing protected areas network in preserving suitable habitats, and to map suitable areas lacking protection. Finally, we assessed whether small felids are good proxies of broader regional terrestrial biodiversity. On the mainland, the largest and most suitable habitats occurred in the Northern Forest Complex of Myanmar and in the region between Eastern Myanmar, Laos and Vietnam. In these areas we also highlighted the most important protected areas. In Borneo, the most suitable habitats occurred in the central highlands and in the protected areas of Sabah. In Sumatra, the strongholds of habitat suitability were the Barisan Mountains, in the western extent of the island, and were highly concentrated within existing protected areas. We also found that the aggregated habitat suitability for small felids was correlated more strongly to terrestrial vertebrate biodiversity than was any single felid species individually, suggesting that areas that are suitable for multiple felid species have an association with high overall biodiversity. Overall, our assessment of the distribution of small felids in Southeast Asia highlights the fundamental importance of protected areas for biodiversity conservation, given that most species were highly associated with protected areas and regions with large extents of forest. Our results are a clarion call to expand the extent, and improve the conservation management, of protected areas in the remaining core habitat areas for multiple species in Southeast Asia, and to work to enhance and protect connectivity between them to ensure long-term demographic and genetic exchange among the region’s remaining wildlife populations.

Choose-your-own-adventure (CYOA) narratives offer immersive experiences that can effectively convey complex conservation concepts, fostering empathy and critical thinking, particularly in addressing issues like human-elephant conflict. Despite their potential, there is limited research on the use of CYOA activities in conservation education. In this study, we created an interactive story centered on elephant conservation, drawing from existing research to distill scientific concepts into engaging narratives and utilizing various modes of delivery (YouTube and live performances) to reach diverse audiences. We conducted postactivity surveys to assess variations in audience perception, learning, and conservation engagement intentions in relation to sociodemographic factors, activity type, and messages encountered. We then modeled the relationships between ordinal responses and explanatory variables using cumulative ordinal mixed models (N = 398). For the YouTube version, we also considered sociodemographic factors (YouTube n = 53, non-YouTube n = 47). Our findings indicate a preference for live performances over online YouTube activity, with participants gaining knowledge about elephant conservation from both formats. For the YouTube activity, participants strongly agreed that the activity allowed engagement with the character and topic. They also expressed a higher likelihood of participating in several conservation actions, relative to a control group. While CYOA storytelling shows promise for conservation education, challenges remain in simplifying scientific language, assessing its impact on comprehension of complex issues, standardizing outcomes, and effectively communicating knowledge. Further research is recommended to adapt this approach, making it applicable to various audiences and domains beyond conservation. "CYOA(choose-your-own-adventure,(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic))"(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic),(sic)(sic)CYOA(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)((sic)(sic)YouTube(sic)(sic)(sic)(sic)(sic))(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(N = 398)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)YouTube(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(YouTube n = 53,(sic)YouTube n 47).(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)YouTube(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic) Youlube(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).CYOA(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).

Serious games are increasingly popular in multiple fields, including education and environmental engagement. We conducted a systematic review to examine the reasons for this increasing popularity, the features of successful serious games and the current trend of serious games application in environmental education. Our systematic search revealed 56 records of papers (from years 2009 to 2023) on the use of serious games for environmental education. Thirty‐five were published in the recent 5 years reflecting a growing interest in this area. The games were aimed at imparting knowledge (N = 28), changing attitude (N = 28) and changing behaviour (N = 19). Reasons for the increased application of serious games include the raising awareness on serious games and growing investment in serious games development and research, as well as better technological access through mobile devices. Successful serious games exhibit the following features: an immersive experience, meaningful engagement, a learn‐by‐doing involvement, simulation of real‐world environmental problems, autonomy in game decisions and the presence of a guiding host. This review also revealed two categories of digital serious games: computer games (N = 14) and mobile apps (N = 11), and three categories of physical games: board games (N = 6), card game (N = 1) and role‐playing games (N = 2). The gaps include (1) the lack of in‐game experience data that limits our understanding of the impacts of commercial games, (2) variation in game experience and conservation experience among participants and how this translates to the impacts of serious games, and (3) lack of understanding of how the effects of serious games with high complexities on adults learning. Serious games are increasingly being digitised and moving towards location‐based games, alternate reality, augmented reality and virtual reality. These games can facilitate interaction between learners and the natural environment, and in turn strengthen environmental awareness and appreciation.

Species Distribution Models (SDMs) are a powerful tool to derive habitat suitability predictions relating species occurrence data with habitat features. Two of the most frequently applied algorithms to model species-habitat relationships are Generalised Linear Models (GLM) and Random Forest (RF). The former is a parametric regression model providing functional models with direct interpretability. The latter is a machine learning non-parametric algorithm, more tolerant than other approaches in its assumptions, which has often been shown to outperform parametric algorithms. Other approaches have been developed to produce robust SDMs, like training data bootstrapping and spatial scale optimisation. Using felid presence-absence data from three study regions in Southeast Asia (mainland, Borneo and Sumatra), we tested the performances of SDMs by implementing four modelling frameworks: GLM and RF with bootstrapped and non-bootstrapped training data. With Mantel and ANOVA tests we explored how the four combinations of algorithms and bootstrapping influenced SDMs and their predictive performances. Additionally, we tested how scale-optimisation responded to species' size, taxonomic associations (species and genus), study area and algorithm. We found that choice of algorithm had strong effect in determining the differences between SDMs' spatial predictions, while bootstrapping had no effect. Additionally, algorithm followed by study area and species, were the main factors driving differences in the spatial scales identified. SDMs trained with GLM showed higher predictive performance, however, ANOVA tests revealed that algorithm had significant effect only in explaining the variance observed in sensitivity and specificity and, when interacting with bootstrapping, in Percent Correctly Classified (PCC). Bootstrapping significantly explained the variance in specificity, PCC and True Skills Statistics (TSS). Our results suggest that there are systematic differences in the scales identified and in the predictions produced by GLM vs. RF, but that neither approach was consistently better than the other. The divergent predictions and inconsistent predictive abilities suggest that analysts should not assume machine learning is inherently superior and should test multiple methods. Our results have strong implications for SDM development, revealing the inconsistencies introduced by the choice of algorithm on scale optimisation, with GLM selecting broader scales than RF.

The Amazon is one of the most diverse biomes around the globe, currently threatened by economic and industrial development and climate change. Large mammals are keystone species, playing an important role in ecosystem structure and function as ecological engineers, while being highly susceptible to deforestation, habitat degradation, and human exploitation. Using a unifying hierarchical Bayesian spatial approach, we examine the site-use patterns of four large Amazonian Forest mammals and their relationships to anthropogenic factors at a biome-wide scale. Our results showed that species' patterns of site use are correlated with human induced habitat changes, and that this correlation is species-specific. The white-lipped peccary shows highest site-use estimates within strict protected areas, affected by proximity to urban areas and benefiting from indigenous territories, the tapir responding slightly to proximity to burned forested areas, while the giant armadillo and the jaguar were primarily affected by vegetation cover loss; disturbances related to the colonization of the Amazon. Our findings contribute to the understanding of how human-induced environmental changes influence the site-use patterns of these four large mammals, and inform future conservation and land use planning. Transboundary conservation efforts, empowering and integrating native (indigenous and non-indigenous) communities in land governance schemes, involving the private sector and securing the commitment of developed countries are important paths for the protection and sustainability of the globally-crucial Amazon rainforest.

The Eurasian lynx ( Lynx lynx ) has been extinct from the UK since the late medieval period. Recently, the idea of a reintroduction of the species has entered the public sphere. While reintroduction receives support from the public, there is significant opposition to the idea within the UK farming community. This study used a questionnaire survey to investigate the attitudes of UK farmers towards the reintroduction of the lynx. The theory of planned behavior was also used to predict farmers’ intention to cull lynx in the case of a reintroduction and the drivers behind this. Results indicated negative attitudes towards reintroduction among UK farmers. Older people, those from potential reintroduction areas, and sheep farmers, those whose income is dependent on farming and those with higher perceived knowledge of lynx, had more negative attitudes. Women were significantly more afraid of lynx than men. We also revealed that many farmers intended to hunt lynx in the case of a reintroduction, with attitude, subjective norm, and perceived behavioral control being significant predictors of intended behavior. Stronger protective laws for lynx could reduce intention to cull. Additionally, active management and regulation of the lynx population can facilitate harmonious coexistence. These findings suggest that high levels of legal protection and strategies that aim to encourage social norms against illegal culling of lynx, reduce capability to kill lynx (through policies relating to the use of equipment that can be used to kill lynx), or aim to change attitudes to culling lynx could help reduce the perceived conflict of farmers with regard to lynx reintroduction and increase the success of a reintroduction.

Deforestation is a major threat to terrestrial tropical ecosystems, particularly in Southeast Asia where human activities have dramatic consequences for the survival of many species. However, responses of species to anthropogenic impact are highly variable. In order to establish effective conservation strategies, it is critical to determine a species’ ability to persist in degraded habitats. Here, we used camera trapping data to provide the first insights into the temporal and spatial distribution of southern pig-tailed macaques (Macaca nemestrina, listed as ‘Vulnerable’ by the IUCN) across intact and degraded forest habitats in Peninsular Malaysia, with a particular focus on the effects of clear-cutting and selective logging on macaque occupancy. Specifically, we found a 10% decline in macaque site occupancy in the highly degraded Pasoh Forest Reserve from 2013 to 2017. This may be strongly linked to the macaques’ sensitivity to intensive disturbance through clear-cutting, which significantly increased the probability that M. nemestrina became locally extinct at a previously occupied site. However, we found no clear relationship between moderate disturbance, i.e., selective logging, and the macaques’ local extinction probability or site occupancy in the Pasoh Forest Reserve and Belum-Temengor Forest Complex. Further, an identical age and sex structure of macaques in selectively logged and completely undisturbed habitat types within the Belum-Temengor Forest Complex indicated that the macaques did not show increased mortality or declining birth rates when exposed to selective logging. Overall, this suggests that low to moderately disturbed forests may still constitute valuable habitats that support viable populations of M. nemestrina, and thus need to be protected against further degradation. Our results emphasize the significance of population monitoring through camera trapping for understanding the ability of threatened species to cope with anthropogenic disturbance. This can inform species management plans and facilitate the development of effective conservation measures to protect biodiversity.

Rates of biodiversity loss in Southeast Asia are among the highest in the world, and the Indo-Burma and South-Central China Biodiversity Hotspots rank among the world’s most threatened. Developing robust multi-species conservation models is critical for stemming biodiversity loss both here and globally. We used a large and geographically extensive remote-camera survey and multi-scale, multivariate optimization species distribution modelling to investigate the factors driving biodiversity across these two adjoining biodiversity hotspots. Four major findings emerged from the work. (i) We identified clear spatial patterns of species richness, with two main biodiverse centres in the Thai-Malay Peninsula and in the mountainous region of Southwest China. (ii) Carnivores in particular, and large ungulates to a lesser degree, were the strongest indicators of species richness. (iii) Climate had the largest effect on biodiversity, followed by protected status and human footprint. (iv) Gap analysis between the biodiversity model and the current system of protected areas revealed that the majority of areas supporting the highest predicted biodiversity are not protected. Our results highlighted several key locations that should be prioritized for expanding the protected area network to maximize conservation effectiveness. We demonstrated the importance of switching from single-species to multi-species approaches to highlight areas of high priority for biodiversity conservation. In addition, since these areas mostly occur over multiple countries, we also advocate for a paradigmatic focus on transboundary conservation planning.

The persistent high deforestation rate and fragmentation of the Amazon forests are the main threats to their biodiversity. To anticipate and mitigate these threats, it is important to understand and predict how species respond to the rapidly changing landscape. The short-eared dog Atelocynus microtis is the only Amazon-endemic canid and one of the most understudied wild dogs worldwide. We investigated short-eared dog habitat associations on two spatial scales. First, we used the largest record database ever compiled for short-eared dogs in combination with species distribution models to map species habitat suitability, estimate its distribution range and predict shifts in species distribution in response to predicted deforestation across the entire Amazon (regional scale). Second, we used systematic camera trap surveys and occupancy models to investigate how forest cover and forest fragmentation affect the space use of this species in the Southern Brazilian Amazon (local scale). Species distribution models suggested that the short-eared dog potentially occurs over an extensive and continuous area, through most of the Amazon region south of the Amazon River. However, approximately 30% of the short-eared dog's current distribution is expected to be lost or suffer sharp declines in habitat suitability by 2027 (within three generations) due to forest loss. This proportion might reach 40% of the species distribution in unprotected areas and exceed 60% in some interfluves (i.e. portions of land separated by large rivers) of the Amazon basin. Our local-scale analysis indicated that the presence of forest positively affected short-eared dog space use, while the density of forest edges had a negative effect. Beyond shedding light on the ecology of the short-eared dog and refining its distribution range, our results stress that forest loss poses a serious threat to the conservation of the species in a short time frame. Hence, we propose a re-assessment of the short-eared dog's current IUCN Red List status (Near Threatened) based on findings presented here. Our study exemplifies how data can be integrated across sources and modelling procedures to improve our knowledge of relatively understudied species.

Anthropogenic habitat alteration and invasive species are threatening carnivores globally. Understanding the impact of these factors is critical for creating localized, effective conservation programmes. Madagascar's Eupleridae have been described as the least studied and most threatened group of carnivores. We investigated the effects of habitat degradation and the presence of people and exotic species on the modelled occupancy of the endemic fosa Cryptoprocta ferox, conducting camera-trap surveys in two western deciduous forests, Ankarafantsika National Park and Andranomena Special Reserve. Our results indicated no clear patterns between habitat degradation and fosa occupancy but a strong negative association between cats Felis sp. and fosas. Cat occupancy was negatively associated with birds and positively associated with contiguous forest and narrow trails. In contrast, dog Canis lupus familiaris occupancy was best predicted by wide trails, degraded forest and exotic civets. Our results suggest fosas are capable of traversing degraded landscapes and, in the short term, are resilient to contiguous forest disturbance. However, high occupancy of cats and dogs in the landscape leads to resource competition through prey exploitation and interference, increasing the risk of transmission of potentially fatal diseases. Management strategies for exotic carnivores should be considered, to reduce the widespread predation of endemic species and the transmission of disease.

Palliative chemotherapy is the standard of care for patients with advanced nasopharyngeal carcinoma. Radiotherapy may be offered for patients who responded to chemotherapy for local control. However, the dose and fractionation schedules used varies between oncologists. An online survey via email was conducted for all practicing oncologists in public hospitals in Malaysia. The questionnaires were sent to 42 oncologists. The questionnaires gathered data on their practice in giving radiotherapy to patients with advanced nasopharyngeal carcinoma who responded to palliative chemotherapy,. 33 oncologists responded to the questionnaire, which gave a response rate of 78.5%. Most oncologists (85%) will give radiotherapy if patients responded to palliative chemotherapy, and 90.9% will give radiotherapy alone without concurrent chemotherapy. The radiotherapy dose fractionation schedules vary among the oncologists, with 55Gy/20 fractions/4 weeks the commonest fractionation used (30.3%), followed by 40Gy/15 fractions/3 weeks (24.2%), and 9.1% choosing schedules such 30Gy/10 fractions/2 weeks, 45Gy/15 fractions/3 weeks, and 70Gy/35 fractions/7 weeks. The other fractionations used are 52Gy/13 fractions/2.5 weeks, 50Gy/25 fractions/5 weeks, 60Gy/25 fractions/5 weeks, 66Gy/33 fractions/6.5 weeks, and 60Gy/30 fractions/6 weeks. Most oncologists will prefer a fractionation schedules of 3-4 weeks duration (75.8%), with high enough dose to give a good local control and avoiding long treatment duration, as the radiotherapy is non-curative in nature. There are currently no guidelines or consensus on the optimum radiotherapy dose fractionation schedules for this group of patients. The decision for the preferred dose-fractionation schedules have to take into consideration factors such as symptoms, disease extent and comorbidity. An optimum dose-fractionation schedule which does not prolong the treatment duration and giving the best local control is very much needed, especially in situations such as the current COVID-19 pandemic.

In Malaysia, up to 68% of cancer patients uses CAM, with CAM disclosure at only 7.6%. Perception of doctors’ poor knowledge on CAM, and non-belief in its practice are some reasons cited for non-disclosure. This may lead to various complications. This cross-sectional study was carried out in 2 university hospitals in Malaysia from 1st July to 23rd December 2016. All doctors and nurses of Oncology Departments were included. Subjects were briefed on the study before questionnaires were distributed and returned anonymously once completed. The questionnaire was adopted with local adaptations, from a study done in Japan on perceptions and attitudes on CAM. A pilot study was done and the final validated version consists of 2 sections. Section A consists of questions on socio-demographic characteristics of respondent. Section B consisted of questions to assess the respondent’s knowledge, perception and attitude on CAM. Each question was given a point scale. Based on total points obtained, respondents were classified into good or poor knowledge, positive or negative perception and attitude towards CAM. Total of 60 nurses and 26 doctors responded, with 16 male and 70 female. Generally, all respondents have poor knowledge on CAM. Two thirds (67.4%) were not aware of the existence of the National Policy on CAM in Malaysia. Most have very little or no knowledge on the CAM modalities listed. The respondents have negative perception on CAM, with 83.7% feel the lack of evidence in its effectiveness. Most (72%) think that there is a definite or possible interaction between CAM products and anticancer drugs. All have negative attitude on CAM except 8.1% of respondents encouraging the use of CAM. Majority do not encourage the use of CAM products in both early (79.1%) and advanced (64%) stage. Oncology-related healthcare providers have poor knowledge, with negative perception and attitude towards CAM.

Amazonia forest plays a major role in providing ecosystem services for human and sanctuaries for wildlife. However, ongoing deforestation and habitat fragmentation in the Brazilian Amazon has threatened both. The ocelot is an ecologically important mesopredator and a potential conservation ambassador species, yet there are no previous studies on its habitat preference and spatial patterns in this biome. From 2010 to 2017, twelve sites were surveyed, totaling 899 camera trap stations, the largest known dataset for this species. Using occupancy modeling incorporating spatial autocorrelation, we assessed habitat use for ocelot populations across the Brazilian Amazon. Our results revealed a positive sigmoidal correlation between remote-sensing derived metrics of forest cover, disjunct core area density, elevation, distance to roads, distance to settlements and habitat use, and that habitat use by ocelots was negatively associated with slope and distance to river/lake. These findings shed light on the regional scale habitat use of ocelots and indicate important species-habitat relationships, thus providing valuable information for conservation management and land-use planning.

Western Madagascar's deciduous forest is an important, threatened habitat to little-known Eupleridae, such as the fosa Cryptoprocta ferox. Using camera-trap grids established in two important deciduous forests, Ankarafantsika National Park and Andranomena Special Reserve, we explore the activity patterns of endemic and exotic carnivores and evaluate how exotics may be influencing fosa temporal activity across these two protected areas. Three methodologies, kernel density, circular and wave analysis, were used to evaluate the activity pattern overlap between exotic free-ranging cats Felis sp. and dogs Canis familiaris, exotic small Indian civets Viverricula indica and the endemic fosa. Our results showed fosas to be cathemeral, peaking in activity in the evening and at dawn. Cats were nocturnal, whilst also highly active at dusk. Civets did not display a dominant period of activity, but were relatively more active in the night, and at dusk. In contrast, dogs were diurnal and also active at dawn, in alignment with human activity. Fosa activity significantly overlapped with the nocturnal cat and civet, whilst being distinct from the diurnal dog and humans. Our results suggest that there is potentially a competitive impact of exotic free-ranging dogs and cats when present across fosas' dry forest habitat. This highlights the need for further examination into the impact of Madagascar's exotic carnivores, and the experimental trial to investigate the impact of their removal (or reduced abundance), on Madagascar's wildlife.

Aim Deforestation is rapidly altering Southeast Asian landscapes, resulting in some of the highest rates of habitat loss worldwide. Among the many species facing declines in this region, clouded leopards rank notably for their ambassadorial potential and capacity to act as powerful levers for broader forest conservation programmes. Thus, identifying core habitat and conservation opportunities are critical for curbing further Neofelis declines and extending umbrella protection for diverse forest biota similarly threatened by widespread habitat loss. Furthermore, a recent comprehensive habitat assessment of Sunda clouded leopards (N. diardi) highlights the lack of such information for the mainland species (N. nebulosa) and facilitates a comparative assessment. Location Southeast Asia. Methods Species-habitat relationships are scale-dependent, yet <5% of all recent habitat modelling papers apply robust approaches to optimize multivariate scale relationships. Using one of the largest camera trap datasets ever collected, we developed scale-optimized species distribution models for two con-generic carnivores, and quantitatively compared their habitat niches. Results We identified core habitat, connectivity corridors, and ranked remaining habitat patches for conservation prioritization. Closed-canopy forest was the strongest predictor, with similar to 25% lower Neofelis detections when forest cover declined from 100 to 65%. A strong, positive association with increasing precipitation suggests ongoing climate change as a growing threat along drier edges of the species' range. While deforestation and land use conversion were deleterious for both species, N. nebulosa was uniquely associated with shrublands and grasslands. We identified 800 km(2) as a minimum patch size for supporting clouded leopard conservation. Main conclusions We illustrate the utility of multi-scale modelling for identifying key habitat requirements, optimal scales of use and critical targets for guiding conservation prioritization. Curbing deforestation and development within remaining core habitat and dispersal corridors, particularly in Myanmar, Laos and Malaysia, is critical for supporting evolutionary potential of clouded leopards and conservation of associated forest biodiversity.

Management of tigers is a response to a global conservation crisis. Range contraction, population decline, habitat fragmentation, prey loss, and poaching cause and aggravate this crisis. There is a debate as to whether conserving source sites or maintaining landscape connectivity is the greater priority. Both approaches rely on understanding environmental and anthropogenic factors. We used a large dataset from a nation-wide camera trapping survey to investigate factors affecting habitat use of tigers across the montane landscape of Bhutan. We tested the effects of environmental and anthropogenic covariates on habitat use while accounting for imperfect detection using a hierarchical occupancy model. Tiger habitat use showed a strong positive association with closeness to protected area, greater distance from human settlement and availability of abundant large prey. Our findings can help to identify the drivers of tiger decline and contribute to the refinement of conservation strategies to combat that decline in this montane conservation landscape. We provide a general approach to tiger conservation by highlighting the importance of understanding the basic ecology of this apex predator, including the relationship between environmental and anthropogenic variables and habitat use. Bhutan, with unusually strong environmental legislation, and a high percentage of its area protected and under contiguous forest cover, set the global standard in large carnivore conservation and is an important component of a wider conservation complex in South Asia.

The survival of large carnivores is increasingly precarious due to extensive human development that causes the habitat loss and fragmentation. Habitat selection is influenced by anthropogenic as well as environmental factors, and understanding these relationships is important for conservation management. We assessed the environmental and anthropogenic variables that influence site use of clouded leopard Neofelis nebulosa in Bhutan, estimated their population density, and used the results to predict the species' site use across Bhutan. We used a large camera-trap dataset from the national tiger survey to estimate for clouded leopards, for the first time in Bhutan, (1) population density using spatially explicit capture-recapture models and (2) site-use probability using occupancy models accounting for spatial autocorrelation. Population density was estimated at D = 0.40 (0.10 SD) and D maximum-likelihood=0.30 (0.12 SE) per 100km(2). Clouded leopard site use was positively associated with forest cover and distance to river while negatively associated with elevation. Mean site-use probability (from the Bayesian spatial model) was =0.448 (0.076 SD). When spatial autocorrelation was ignored, the probability of site use was overestimated, =0.826 (0.066 SD). Predictive mapping allowed us to identify important conservation areas and priority habitats to sustain the future of these elusive, ambassador felids and associated guilds. Multiple sites in the south, many of them outside of protected areas, were identified as habitats suitable for this species, adding evidence to conservation planning for clouded leopards in continental South Asia.