Anthropogenic changes are impacting biodiversity at multiple levels. However, the effects of environmental stressors on physiological function and health of individual organisms are not well understood. Population-specific benchmark data are crucial for evaluating the health of populations over time, and for comparisons between different wild populations and captive animals. The Fosa (Cryptoprocta ferox), the largest extant member of the Eupleridae, is endemic to Madagascar. As an apex predator, the Fosa faces increasing anthropogenic disturbance. It therefore is vital to establish reference intervals for hematological and biochemical parameters in free-ranging Fosa to monitor their health and to conserve the species in both wild and captive environments. Through a sequence of blood tests, we aimed to provide information on the physiological health of free-ranging Fosa and in comparison with captive Fosa. A total of 71 Fosa from 3 sites across their geographic range were captured and sampled for hematological and serum biochemical analysis for health evaluation. Our analysis indicates that many wild Fosa have blood values outside the RI established from captive individuals, which may reflect stress and pathological conditions of individuals and underscores the importance of establishing population-specific reference intervals because those derived from captive individuals may not accurately reflect the health status of wild populations. The reference intervals obtained through our study have the potential to serve as valuable benchmarks for future evaluations of hematologic and serum biochemistry values in wild Fosa, contributing to future research and conservation efforts. Our study establishes key health benchmarks for the Fosa, Madagascar's top predator, by analyzing blood samples from wild populations. These insights can help assess how human disturbances affect their health, aiding future conservation efforts for this endangered species. Les changements anthropiques ont un impact sur la biodiversit & eacute; & agrave; plusieurs niveaux. Cependant, les effets des facteurs de stress environnementaux sur la fonction physiologique et la sant & eacute; des animaux sauvages & agrave; l'& eacute;chelle individuelle ne sont pas bien compris. Les donn & eacute;es de r & eacute;f & eacute;rence sp & eacute;cifiques & agrave; la population sont cruciales pour & eacute;valuer la sant & eacute; des populations au fil du temps et pour les comparaisons entre diff & eacute;rentes populations sauvages et les animaux en captivit & eacute;. En tant que pr & eacute;dateur au sommet de la cha & icirc;ne alimentaire, le Fosa (Cryptoprocta ferox), est confront & eacute; & agrave; des perturbations anthropiques croissantes. Il est donc essentiel d'& eacute;tablir des intervalles de r & eacute;f & eacute;rence (IR) pour les param & egrave;tres h & eacute;matologiques et biochimiques chez les Fosa en libert & eacute; afin de surveiller leur sant & eacute; et de conserver l'esp & egrave;ce dans les environnements sauvages et en captivit & eacute;. Gr & acirc;ce & agrave; une s & eacute;rie de tests sanguins, nous avons cherch & eacute; & agrave; fournir des informations sur la sant & eacute; physiologique des Fosa en libert & eacute; et en comparaison avec les Fosa en captivit & eacute;. Au total, 71 Fosa provenant de trois sites r & eacute;partis dans l'aire de r & eacute;partition g & eacute;ographique de l'esp & egrave;ce ont & eacute;t & eacute; captur & eacute;s et & eacute;chantillonn & eacute;s pour une analyse h & eacute;matologique et biochimique du s & eacute;rum en vue d'une & eacute;valuation de la sant & eacute;. Notre analyse indique que de nombreux Fosa sauvages pr & eacute;sentent des valeurs sanguines en dehors des RI & eacute;tablies & agrave; partir d'individus en captivit & eacute;, ce qui peut refl & eacute;ter le stress et les conditions pathologiques des individus. Les intervalles de r & eacute;f & eacute;rence obtenus gr & acirc;ce & agrave; notre & eacute;tude ont le potentiel de servir de rep & egrave;res pr & eacute;cieux pour les futures & eacute;valuations des valeurs h & eacute;matologiques et biochimiques s & eacute;riques chez les Fosa sauvages, contribuant ainsi aux futurs efforts de recherche et de conservation.

Madagascar is acclaimed for its floral and faunal endemism and biodiversity. Among the island nation's most emblematic fauna are its native mammalian carnivores; they are members of the threatened and endemic Eupleridae family. The Corridor of Marojejy, Anjanaharibe-Sud Special Reserve, Tsaratanana (COMATSA) is a newly protected corridor system that faces deforestation and lacks detailed assessments of its native carnivore community. We deployed 44 motion-activated trail cameras to identify which terrestrial carnivores are found within the rainforest corridor landscape of COMATSA-Sud and Marojejy National Park, and to examine carnivore occupancy, relative activity and the impacts of habitat edge. Our sampling array operated from October 20, 2021, to February 10, 2022 (113 days) and confirmed the presence of four species in Eupleridae: Cryptoprocta ferox, Fossa fossana, Galidia elegans, and Galidictis fasciata, as well as a non-native carnivore species: Canis familiaris. We show that carnivore occurrences significantly increase with distance to forest edges. We discuss the negative impacts of forest loss on native carnivores and highlight the threats posed by the non-native carnivore C. familiaris. Our findings spotlight faunal detections across the corridor and indicate that fewer native carnivores are found in the corridor compared to nearby protected areas, suggesting that deforestation correlates with a skewed distribution of carnivore detections. Based on our data, we provide management recommendations for the protection of the forest corridor and its endemic faunal community. Madagascar is known for its unique biodiversity, including the threatened and endemic Eupleridae family of carnivores. Our study in the COMATSA corridor used 44 trail cameras to identify carnivore species, revealing significant findings about their presence. We found that native carnivore occurrences increase with distance from forest edges and that deforestation negatively impacts these species, prompting management recommendations for their protection.image

The IUCN Red List of Threatened Species is an extinction risk assessment tool that has guided species conservation over the last five decades. However, as wildlife scientists and conservationists, we argue that its influence on the global conservation agenda can hinder effective species conservation efforts. Here, we review the limitations of the Red List and its misuse in priority setting, which can overlook local and regional contexts. This can result in improper allocation of conservation resources, especially in the Global South, where financial resources are limited. In particular, funds directed towards red-listed species may fail to address a broader range of conservation priorities. We also contend that extinction risk is insufficient for guiding conservation efforts and recommend broadening conservation planning and decision-making beyond reliance on the Red List. Thus, for a more inclusive and decentralized approach, we summarize guidelines for guiding species conservation at appropriate ecological, spatial, and taxonomic scales. Finally, we encourage more collaborative efforts and stakeholder engagement for the setting of conservation priorities and efficient funding allocation.

The Vulnerable fosa Cryptoprocta ferox is the largest native carnivore in Madagascar, fulfilling a unique ecological niche in the island's remaining forests. Negative interactions with humans threaten the long-term viability of most remaining fosa populations across Madagascar. Threats to the fosa include habitat loss and persecution by humans resulting from perceived predation on domestic animals. We used GPS collars to record space use and activity patterns of five fosas in Ankarafantsika National Park, Madagascar, during the dry seasons of 2016 and 2017. The results, with up to 2,110 recorded locations per individual, indicated fosas’ home ranges and movements were not limited to the forest, and all collared individuals used networks of habitat patches and corridors to navigate deforested areas. The fosas studied in Ankarafantsika National Park had significantly larger home ranges than those reported in previous studies in other protected areas. They were rarely found within village boundaries and appeared to avoid areas of human habitation, suggesting that during the study period livestock was not a significant component of the fosas’ diet in this Park. Our results suggest that fosas have some flexibility that enables them to adapt to living near deforested and human-dominated areas by altering their space-use patterns, but they are compensating by increasing their home range size.

Conservation officials work to manage complex and interacting human-environmental systems, where balancing needs between the two systems can become a source of tension. This study presents information on the use of natural resources by, and the health and welfare of, rural communities within and near Ankarafantsika National Park (ANP) in northwestern Madagascar. We focus on behaviors that are difficult for natural resource managers to measure themselves, including the hunting of threatened and protected wildlife and on sensitive information about humanwealth, health, and food security. We surveyed 41 9 households and measured the health of 1 860 individuals in 1 8 communities adjacent to or within the boundaries of ANP. We found a very high prevalence of child malnutrition, illness, and food insecurity and a heavy reliance on natural products to meet subsistence needs. More than 90% of the population reported that they hunted wildlife and harvested wild vegetables at least one day during the prior week as a direct means to cope with their food insecurity. Further, we found a high reliance on the forest for both healthcare and the building of adequate shelter. Efforts to improve overall food security would likely improve both human welfare and the long-term conservation of the threatened wildlife and habitat of Ankarafantsika. These data can help both conservation and community livelihood programs to find integrated solutions to the shared challenges of improving the well-being of human populations and the protection of Madagascar’s unique, endemic, and highly threatened biodiversity. Les gestionnaires oeuvrant pour la protection de la nature sont généralement confrontés à des systèmes socio-écologiques complexes et interactifs dans lesquels la recherche de l’équilibre entre les besoins de ces deux systèmes peut s’avérer être une source de tension. Cette étude présente des informations sur l'utilisation des ressources naturelles par les communautés rurales riveraines du parc national d'Ankarafantsika (PNA) dans le nord-ouest de Madagascar, ainsi que sur la santé et le bien-être de ces communautés. L’étude s’est en particulier orientée sur les comportementsdifficiles à mesurer pour les gestionnaires de ressources naturelles, à savoir la chasse d'animaux sauvages menacés et protégés et les informations portant sur l’opulence, la santé et la sécurité alimentaire des gens. Une enquête a été réalisée auprès de 41 9 ménages et l’état de santé de 1 860 personnes a été mesuré dans 1 8 communautés vivant à la périphérie ou à l’intérieur des limites du PNA. Une très forte prévalence de la malnutrition infantile a été observée ainsi que diverses pathologies, une insécurité alimentaire et une dépendance importante à l'égard des produits naturels pour répondre aux besoins de subsistance. Plus de 90% de la population a déclaré qu'elle avait chassé des animaux et récolté des plantes sauvages au moins un jour au cours de la semaine précédente, à titre de moyen direct pour faire face à l' insécurité alimentaire. Une forte dépendance à l'égard des forêts a également été notée pour les produits destinés à la santé et la construction de maisons. Les efforts visant à améliorer la sécurité alimentaire dans son ensemble pourraient vraisemblablement améliorer le bien-être humain aussi bien que la conservationà long terme de la faune et des habitats menacés de l'Ankarafantsika. Ces données peuvent aider les programmes de conservation et de subsistance de la communauté à trouver des solutions intégrées aux problèmes communs de l’amélioration du bien-être des populations humaines et de la protection de la biodiversité unique, endémique et hautement menacée de Madagascar.

Madagascar does not have native wild felid species; however, distinct populations of free-ranging “forest cats” of unknown species are known throughout the island, including at Ankarafantsika National Park, Bezà Mahafaly Special Reserve, Makira Natural Park and the Masoala peninsula. Malagasy “forest cats” are commonly considered invasive lemur predators and competitors with endemic carnivores as well as a nuisance exotic species that kill poultry. These cats may be descendants of African wildcats, European wildcats and/or domestic cats; however, no research on their genetic origin has been published. To determine their taxonomic status, genetic data from short tandem repeat markers was assessed for three wild-caught “forest cats” from the Bezà Mahafaly Special Reserve (BMSR) and 27 “forest cats” from Ankarafantsika National Park. Bayesian analyses comparing the Malagasy “forest cats” to approximately 1900 domestic and wildcat sub-species suggest the Malagasy cats are descendents of domestic cats from the Arabian Sea region, including the islands of Lamu and Pate, Dubai, Kuwait and Oman. Additional genetic influences may descend from India and Pakistan. Combined with cultural and historical information, these data suggest that these felid populations are likely descendents from cats that immigrated to the island on trade ships, particularly along early Arab trade routes.

Anthropogenic habitat alteration and invasive species are threatening carnivores globally. Understanding the impact of these factors is critical for creating localized, effective conservation programmes. Madagascar's Eupleridae have been described as the least studied and most threatened group of carnivores. We investigated the effects of habitat degradation and the presence of people and exotic species on the modelled occupancy of the endemic fosa Cryptoprocta ferox, conducting camera-trap surveys in two western deciduous forests, Ankarafantsika National Park and Andranomena Special Reserve. Our results indicated no clear patterns between habitat degradation and fosa occupancy but a strong negative association between cats Felis sp. and fosas. Cat occupancy was negatively associated with birds and positively associated with contiguous forest and narrow trails. In contrast, dog Canis lupus familiaris occupancy was best predicted by wide trails, degraded forest and exotic civets. Our results suggest fosas are capable of traversing degraded landscapes and, in the short term, are resilient to contiguous forest disturbance. However, high occupancy of cats and dogs in the landscape leads to resource competition through prey exploitation and interference, increasing the risk of transmission of potentially fatal diseases. Management strategies for exotic carnivores should be considered, to reduce the widespread predation of endemic species and the transmission of disease.

Western Madagascar's deciduous forest is an important, threatened habitat to little-known Eupleridae, such as the fosa Cryptoprocta ferox. Using camera-trap grids established in two important deciduous forests, Ankarafantsika National Park and Andranomena Special Reserve, we explore the activity patterns of endemic and exotic carnivores and evaluate how exotics may be influencing fosa temporal activity across these two protected areas. Three methodologies, kernel density, circular and wave analysis, were used to evaluate the activity pattern overlap between exotic free-ranging cats Felis sp. and dogs Canis familiaris, exotic small Indian civets Viverricula indica and the endemic fosa. Our results showed fosas to be cathemeral, peaking in activity in the evening and at dawn. Cats were nocturnal, whilst also highly active at dusk. Civets did not display a dominant period of activity, but were relatively more active in the night, and at dusk. In contrast, dogs were diurnal and also active at dawn, in alignment with human activity. Fosa activity significantly overlapped with the nocturnal cat and civet, whilst being distinct from the diurnal dog and humans. Our results suggest that there is potentially a competitive impact of exotic free-ranging dogs and cats when present across fosas' dry forest habitat. This highlights the need for further examination into the impact of Madagascar's exotic carnivores, and the experimental trial to investigate the impact of their removal (or reduced abundance), on Madagascar's wildlife.

Fosas (Cryptoprocta ferox) are Madagascar's largest carnivores, occupying much of the island's forested landscape. This study provides the first evaluation of fosas' conflict with humans, a problem for many small and medium sized carnivores worldwide. We examined fosas' predation of poultry, and the subsequent retaliatory killing. Over 1750 households were interviewed across four regions, encompassing Madagascar's major forest types (deciduous/rainforest) and protected area classifications (national park, reserve and unprotected forest). Predation by fosa was the third highest reported cause (15%) of poultry mortality, with little evidence that coops were effective in reducing predation. Predation of poultry was more prevalent in deciduous forests, and most common during the evenings of the dry season. Over half of all interviewees said they disliked fosas, with loss of poultry the most commonly stated reason. Respondents' that had suffered poultry depredation and those with lower educational attainment were more likely to dislike fosas. Interviewees that disliked fosas and those that were wealthier were most likely to report having killed a fosa. A minimum of thirty fosas was killed in retaliation by our respondents during the year before the interviews. Given that the fosa population is in decline, and most of Madagascar's forests are likely to be too small to support sustainable populations, these killings may be detrimental to vulnerable sub-populations. These results shed insight into the cultural perceptions and predation patterns of a medium sized carnivore, with relevance to worldwide human-wildlife conflict of often overlooked smaller carnivores. We suggest that educational programs, guard dogs, poultry disease vaccinations and robust coop construction may be effective for improving attitudes and reducing retaliatory killing.

Bushmeat consumption in Madagascar is increasingly acknowledged as one of the major threats to its wild vertebrates. Nevertheless, few studies have examined the drivers of the consumption of protected versus legally huntable wild species, or examined its variance across Madagascar’s protected and unprotected areas. This research provides a novel study of the consumption of protected, unprotected, and fish/eel species between forest types (deciduous and rainforest), as well as across a gradient of protected habitat (National Park, Reserve, Unprotected). Members of 1750 households were interviewed across four regions, including two national parks, two reserves, and two unprotected forests. Household demographic, socioeconomic, cultural, and geographic variables were explored as possible predictors of bushmeat consumption. We found that poorer households reported consuming greater quantities of protected species whereas wealthier households reported consuming greater quantities of fish and eel. Households located inside Andasibe-Mantadia National Park, Madagascar’s most visited protected area, reported consuming the greatest quantities of protected species. Interviewees’ most favoured meat was from livestock, and fish. The consumption pattern of wild species reflected interviewees’ stated preference for species that are either unlisted (e.g. tilapia fish) under Malagasy species protection laws, classified as pest (e.g. bushpig) and/or game species (e.g. tenrec). Most protected species (such as lemurs and carnivorans) were interviewees’ least favoured wild meats. Given the lack of cultural affinity, and low preference for the consumption of most protected species, our results suggest that improving accessibility to domestic meat is a promising strategy for reducing the consumption of protected species.

1. Environmental Studies Program, Voinovich School of Leadership and Public Affairs, and Department of Biological Sciences, Ohio University, Athens, OH 45701 USA. 2. Department of Environment and Sustainability, Catawba College, Salisbury, NC 28144 and Nicholas School of the Environment, Duke University, Durham, NC 27708 USA. 3. Department of Biomedical Sciences, Heritage College of Osteopathic Medicine and Center for Ecology and Evolutionary Studies, Ohio University, Athens, OH 45701 USA.

The leopard’s ( Panthera pardus ) broad geographic range, remarkable adaptability, and secretive nature have contributed to a misconception that this species might not be severely threatened across its range. We find that not only are several subspecies and regional populations critically endangered but also the overall range loss is greater than the average for terrestrial large carnivores. To assess the leopard’s status, we compile 6,000 records at 2,500 locations from over 1,300 sources on its historic (post 1750) and current distribution. We map the species across Africa and Asia, delineating areas where the species is confirmed present, is possibly present, is possibly extinct or is almost certainly extinct. The leopard now occupies 25–37% of its historic range, but this obscures important differences between subspecies. Of the nine recognized subspecies, three ( P. p. pardus, fusca, and saxicolor ) account for 97% of the leopard’s extant range while another three ( P. p. orientalis, nimr, and japonensis ) have each lost as much as 98% of their historic range. Isolation, small patch sizes, and few remaining patches further threaten the six subspecies that each have less than 100,000 km 2 of extant range. Approximately 17% of extant leopard range is protected, although some endangered subspecies have far less. We found that while leopard research was increasing, research effort was primarily on the subspecies with the most remaining range whereas subspecies that are most in need of urgent attention were neglected.

Introduced animals impact endemic populations through predation, competition, and disease transmission. Populations of endemic carnivores in Madagascar are declining, and pathogens transmitted from introduced species may further endanger these unique species. We assessed the exposure of introduced and endemic carnivores to common viral and parasitic pathogens in two national parks of Madagascar (Kirindy Mitea National Park and Ankarafantsika National Park) and their neighboring villages. We also identified variables associated with the presence of antibodies to these pathogens in fosa ( Cryptoprocta ferox ). Introduced and endemic species were exposed to canine parvovirus, canine herpesvirus, feline calicivirus, and Toxoplasma gondii . Domestic dogs ( Canis familiaris ) and cats ( Felis catus ) may be sources of infection for these pathogens. Prevalence of antibodies to Toxoplasma in captured fosa was >93%, and adults were more likely to be exposed than immature individuals. Our data provide a basis upon which to evaluate and manage risks of pathogen transmission between species.

Summary In dryland ecosystems, mobility is essential for both wildlife and people to access unpredictable and spatially heterogeneous resources, particularly in the face of climate change. Fences can prevent connectivity vital for this mobility. There are recent calls for large‐scale barrier fencing interventions to address human–wildlife conflict and illegal resource extraction. Fencing has costs and benefits to people and wildlife. However, the evidence available for facilitating sound decision‐making for fencing initiatives is limited, particularly for drylands. We identify six research areas that are key to informing evaluations of fencing initiatives: economics, edge permeability, reserve design, connectivity, ecosystem services and communities. Policy implications. Implementing this research agenda to evaluate fencing interventions in dryland ecosystems will enable better management and policy decisions. The United Nations Conventions on Migratory Species (CMS) and to Combat Desertification (UNCCD) are appropriate international agreements for moving this agenda forward and leading the development of policies and guidelines on fencing in drylands.

Historical knowledge and recent surveys attest that lions are declining across parts of Africa (1, 2). We applaud Bauer et al. (3) for assembling available counts because they motivate better monitoring and conservation support. Their own data, however, rejects their claims that lions are “declining everywhere, except in four southern countries” and that lions increase only where “intensively managed.”

We define African savannahs as being those areas that receive between 300 and 1,500 mm of rain annually. This broad definition encompasses a variety of habitats. Thus defined, savannahs comprise 13.5 million km(2) and encompass most of the present range of the African lion (Panthera leo). Dense human populations and extensive conversion of land to human use preclude use by lions. Using high-resolution satellite imagery and human population density data we define lion areas, places that likely have resident lion populations. In 1960, 11.9 million km(2) of these savannahs had fewer than 25 people per km(2). The comparable area shrank to 9.7 million km(2) by 2000. Areas of savannah Africa with few people have shrunk considerably in the last 50 years and human population projections suggest they will likely shrink significantly in the next 40. The current extent of free-ranging lion populations is 3.4 million km(2) or about 25 % of savannah area. Habitats across this area are fragmented; all available data indicate that between 32,000 and 35,000 free-ranging lions live in 67 lion areas. Although these numbers are similar to previous estimates, they are geographically more comprehensive. There is abundant evidence of widespread declines and local extinctions. Under the criteria we outline, ten lion areas qualify as lion strongholds: four in East Africa and six in Southern Africa. Approximately 24,000 lions are in strongholds, with an additional 4,000 in potential ones. However, over 6,000 lions are in populations of doubtful long-term viability. Lion populations in West and Central Africa are acutely threatened with many recent, local extinctions even in nominally protected areas.

Packer etal. reported that fenced lion populations attain densities closer to carrying capacity than unfenced populations. However, fenced populations are often maintained above carrying capacity, and most are small. Many more lions are conserved per dollar invested in unfenced ecosystems, which avoid the ecological and economic costs of fencing.

We define African savannahs as being those areas that receive between 300 and1,500 mm of rain annually. This broad definition encompasses a variety of habitats. Thusdefined, savannahs comprise 13.5 million km 2 and encompass most of the present range of Electronic supplementary material The online version of this article (doi:10.1007/s10531-012-0381-4)contains supplementary material, which is available to authorized users.J. Riggio A. Jacobson L. Dollar S. Pimm (&)Nicholas School of the Environment, Duke University, Durham, NC 27708, USAe-mail: stuartpimm@me.comJ. Riggio A. Jacobson L. Dollar S. PimmNational Geographic Society, Big Cats Initiative, Washington, DC, USAL. DollarDepartment of Biology, Pfeiffer University, Misenheimer, NC 28109, USAH. Bauer A. DickmanWildlife Conservation Research Unit, University of Oxford, Recanati-Kaplan Centre,Tubney OX13 5QL, UKM. BeckerZambian Carnivore Programme, PO Box 80, Mfuwe, ZambiaM. BeckerDepartment of Ecology, Montana State University, Bozeman, MT 59717, USAP. FunstonDepartment of Nature Conservation, Tshwane University of Technology, Pretoria, South AfricaR. GroomDepartment of Zoology, University of Johannesburg, PO Box 524, Auckland Park 2006, South AfricaR. GroomAfrican Wildlife Conservation Fund, 10564 NW 57th St., Doral, FL 33178, USAP. HenschelPanthera, 8 West 40th Street, 18th Floor, New York, NY 10018, USA