This study reports the isolation and structural characterization of a novel β-fructan (PMP2) from Polygonatum multiflorum using sequential pressing, macroporous resin adsorption, and ultrafiltration (yield: 0.66 %; purity: 93.8 %). Structural analysis revealed a 4.06 kDa polymer with a fructose-dominated composition (Fru/Glu = 86.5:12.9), featuring a linear backbone of →1)-β-D-Fruf-(2→ with →6)-β-D-Fruf-(2→ and →1,6)-β-D-Fruf-(2→ branches, as confirmed by methylation and NMR. SEM revealed lamellar morphology at 400× magnification and porous fibrous structure at 2000×. PMP2 exhibited potent α-amylase inhibition (84.3 % at 0.25 mg/mL) and limited antioxidant activity (e.g., the DPPH scavenging rate was 15.51 ± 1.53 % at 10 mg/mL). Kinetic analysis confirmed competitive inhibition (3.1-fold Km increase; unchanged Vmax), while molecular docking demonstrated high-affinity binding (-17.6 kcal/mol) to the catalytic site through hydrogen bonding and steric blockade. The branching configuration correlates with enhanced enzymatic inhibition, positioning PMP2 as a structurally unique fructan for glycemia-control functional foods. These findings provide foundational data for P. multiflorum utilization.

Background: Epilepsy is a neurological disease, and its clinical treatment presents a significant medical challenge. Nevertheless, Chloriti Lapis (CL), a traditional Chinese Mineral Medicine, has been employed for centuries to address neurological disorders and has shown notable effectiveness. Objectives: The objectives of this study are to further explore whether CL can modulate the abundance and diversity of the small intestinal flora in rats with epilepsy induced by pentylenetetrazol (PTZ) and to investigate the interrelationship among the flora. Materials and Methods: Operational taxonomic units (OTUs), α diversity, β diversity, hierarchical clustering tree, and principal component analysis (PCA) were utilized to analyze the small intestinal flora of PTZ-kindled epileptic rats. Results: The predominant bacterial groups in the small intestine mainly belonged to the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. The administration of CL was found to effectively increase the abundance and diversity of the small intestinal flora, modify its structural composition, reduce the relative abundance of PTZ-elevated microflora, and enhance the abundance of Streptococcus, Staphylococcus, and Gemella Berger. Conclusion: CL exhibits promising potential, reliability, and unique value in managing epilepsy through the regulation of intestinal flora.

Background Epilepsy is a neurological disease, and its clinical treatment presents a significant medical challenge. Nevertheless, Chloriti Lapis (CL), a traditional Chinese Mineral Medicine, has been employed for centuries to address neurological disorders and has shown notable effectiveness. Objectives The objectives of this study are to further explore whether CL can modulate the abundance and diversity of the small intestinal flora in rats with epilepsy induced by pentylenetetrazol (PTZ) and to investigate the interrelationship among the flora. Materials and Methods Operational taxonomic units (OTUs), α diversity, β diversity, hierarchical clustering tree, and principal component analysis (PCA) were utilized to analyze the small intestinal flora of PTZ-kindled epileptic rats. Results The predominant bacterial groups in the small intestine mainly belonged to the phyla Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. The administration of CL was found to effectively increase the abundance and diversity of the small intestinal flora, modify its structural composition, reduce the relative abundance of PTZ-elevated microflora, and enhance the abundance of Streptococcus, Staphylococcus, and Gemella Berger. Conclusion CL exhibits promising potential, reliability, and unique value in managing epilepsy through the regulation of intestinal flora. Keywords Traditional Chinese medicine , Mineral Chinese medicine/mineral drug/mineral medicine , , , epilepsy , pentylenetetrazol , small intestine , gut microbiota , bain-gut axis , mechanism of action

BACKGROUND:The Fructus Ligustri Lucidi, the fruit of Ligustrum lucidum, contains a variety of bioactive compounds, such as flavonoids, triterpenoids, and secoiridoids. The proportions of these compounds vary greatly during the different fruit development periods of Fructus Ligustri Lucidi. However, a clear understanding of how the proportions of the compounds and their regulatory biosynthetic mechanisms change across the different fruit development periods of Fructus Ligustri Lucidi is still lacking.RESULTS:In this study, metabolite profiling and transcriptome analysis of six fruit development periods (45 DAF, 75 DAF, 112 DAF, 135 DAF, 170 DAF, and 195 DAF) were performed. Seventy compounds were tentatively identified, of which secoiridoids were the most abundant. Eleven identified compounds were quantified by high performance liquid chromatography. A total of 103,058 unigenes were obtained from six periods of Fructus Ligustri Lucidi. Furthermore, candidate genes involved in triterpenoids, phenylethanols, and oleoside-type secoiridoid biosynthesis were identified and analyzed. The in vitro enzyme activities of nine glycosyltransferases involved in salidroside biosynthesis revealed that they can catalyze trysol and hydroxytyrosol to salidroside and hydroxylsalidroside.CONCLUSIONS:These results provide valuable information to clarify the profile and molecular regulatory mechanisms of metabolite biosynthesis, and also in optimizing the harvest time of this fruit.

Sparganium stoloniferum is a widely distributed aquatic medicinal plant that exhibits exceptional tolerance to environmental stress. Interestingly, the phenols in S. stoloniferum which have medicinal properties, are defensive compounds. Studies integrating transcriptome, proteome, and metabolome analyses have highlighted the phenylpropanoid and flavonoid biosynthesis pathways, which are related to the defense mechanisms of S. stoloniferum. During the growth process, S. stoloniferum rhizoma (SL) accumulates lignin, forming a physical defense, and phenylpropanoids and flavonoids derived during the lignin biosynthesis process provide chemical defense. In this study, a map of the molecular processes involved in the coordinated defense of S. stoloniferum was generated. The expression patterns of genes involved in the coordinated defense of SL were consistent with the omics results. The cDNA of the phenylalanine ammonia-lyase (PAL) gene from S. stoloniferum (SsPAL) was cloned. The recombinant protein SsPAL showed good affinity and catalytic activity for L-phenylalanine. Heat stress experiments indicated that genes related to phenol biosynthesis were upregulated when S. stoloniferum responded to stress. Therefore, phenols played a crucial defense role when S. stoloniferum was at an early developmental stage or responded to stress in a short time. In contrast, the physical defense mainly relied on lignin formation, which was the main defense mechanism of SL in the late growth stage. Based on these results, we discuss the causes of phenolic pharmacological substance formation in medicinal aquatic plants. These results provide new insights into the defense strategies of aquatic plants.

Background: Realgar is a mineral Chinese medicine, which has been used in China for thousands of years. A well-known truth is that the quality of traditional Chinese medicines is the basis for their efficacious and reliable roles for clinical use, which is principally determined by elements including the location of production and inner inorganic elements composition. However, there are few studies on the quality control of Realgar at present. Colon cancer, a severe malignant tumor, is known to have a poor prognosis due to a high rate of metastasis. So far, the effects of Realgar against colon cancer have not yet been clarified.Methods: An appropriate X-Ray Diffraction (XRD) method was used to systematically analyze the Realgar medic-inal material samples. Fourier fingerprint combined with similarity evaluation, hierarchical cluster analysis, and principal component analysis was performed to assess and classify the samples on account of the XRD Fourier data. Cell viability, wound-healing, transwell migration, and matrigel invasion assays were performed. West-ern blotting was applied to determine the expression of the epithelial-mesenchymal transition (EMT) and MMPs relevant protein levels. Results: Our explorations showed that the XRD Fourier fingerprints of Realgar medicinal material samples that had the same phase revealed 15 and 12 common peaks, respectively. The similarity evaluation of the established XRD Fourier fingerprint was greater than 0.900, which indicated that the XRD Fourier fingerprints of Realgar medicinal material were successfully established. Our study demonstrated that Realgar inhibited the proliferation, migration, and invasion of colon cancer cells. Realgar suppressed the EMT process and decreased MMP-9 and MMP-2 levels, which could be the reason for Realgar to inactivate colon cancer cell migration and invasion.Conclusions: Our findings could be applied in the quality control of Realgar and strongly demonstrate that Realgar could be a potential anti-tumor agent for colon cancer treatment.

OBJECTIVE To establish, analyze and evaluate the inorganic element fingerprint of mineral medicine realgar prepared by water grind processing, and to conduct in vitro anti-colon cancer activity research to explore the spectrum-effect relationship between various inorganic elements and the inhibition of colon cancer cell proliferation.METHODS Inductively coupled plasma-mass spectrometry（ICP-MS） was used to determine the contents of 30 inorganic elements in 12 different batches of realgar prepared by water grind processing to establish inorganic element fingerprints, and the similarity was evaluated. MTT method was used to determine the proliferation inhibition rate of realgar on colon cancer HCT116 cells, and partial least squares regression（PLSR） and gray relational degree（GRA） were combined to analyze the spectrum-effect relationship.RESULTS Among the 30 elements detected, the contents of As, Fe, Sb, Al and Mg were relatively high, while Na, Zn and heavy metals Cu and Cd were not detected, while Pb and Hg were partially detected. The characteristic fingerprints of 12 inorganic elements were established, and the similarity（cosine value of the included angle） was all greater than 0.900 0.The 12 batches of realgar were divided into 2 categories, and the comprehensive evaluation score of realgar from Guizhou ranked in the forefront. MTT experiments showed that realgar inhibited the proliferation of colon cancer HCT116 cells. The partial least squares regression analysis and gray correlation analysis showed that the elements with VIP ＞ 1 and correlation degree ＞ 0.7 were As, Sr, Ga, Ti, Al, Fe, V, Sb, Co, Mg and Mn.CONCLUSION There are certain differences in the types and contents of inorganic elements in different batches of realgar prepared by water grind processing. This study established an accurate and efficient method for analyzing and evaluating inorganic elements in realgar prepared by water grind processing, and the anti-colon cancer effect of realgar is the result of the synergistic action of various elements, mainly closely related to the 11 elements of As, Sr, Ga, Ti, Al, Fe, V, Sb, Co, Mg and Mn.

目的:调查分析华东地区矿物类中药(以下简称矿物药)资源种类、分布及应用现状,为我国矿物药资源发展战略及华东地区区域性矿物药资源产业化发展规划的制定、完善及生产布局提供参考.方法:通过文献检索结合实地调研方式对华东地区矿物药资源进行调查,并对调查数据进行归类和分析.结果:华东地区分布矿物药品种共156种,其中文献资料记载品种141种、现有分布品种138种,文献记载与现有分布共有品种123种.结论:基本查明了华东地区药用矿产资源概况,该区域分布药用矿产资源品种较为丰富,但临床应用品种较少,资源产业化有较大的发展空间;基本厘清了华东地区矿物药资源概况,有助于后续进一步挖掘该地区药用矿产资源的药用价值、发展区域地质经济.

On the basis of the special survey of medicinal animals in the Fourth National Survey of Chinese Materia Medica Resources, according to the diverse ecological types of water bodies and the abundant aquatic medicinal animal resources, combining modern biological technology with traditional survey methods, a survey on the wild resources and aquaculture resources of aquatic medicinal animals was conducted, so as to provide a reference for the formulation and improvement of technical specifications for the general survey of aquatic medicinal animal resources. The problems and formation of germplasm degradation, feed supply shortage, aquaculture environmental pollution in the aquaculture process of aquatic medicinal animals in China were analyzed, and a sustainable development strategy based on the protection of wild resources, with the research of aquatic medicinal animals as the core and ecological aquaculture as the direction was proposed.

Background Quartz album is one of the mineral drugs commonly used in Chinese medicine. Different geogenesis of Quartz album led to differences in its composition and thus affected its clinical efficacy. Mineral Chinese medicines are mainly composed of inorganic elements, including some heavy metals and harmful elements, so their quality control is crucial to their clinical safety. Materials and Methods In this study, the contents of 30 elements in 13 batches Quartz album of different origins were determined by inductively coupled plasma mass spectrometry (ICP-MS). Multiple elements and chemometrics were used to distinguish its different geogenesis. Results We found that the top elements in order of content were as follows: Al>Fe>Ca>K>Mg>Ti>Mn, all with content greater than 100 µg/g. In addition, the contents ranges of Pb, Cd, As, Hg, and Cu were 1.603–637.705, 0.011–1.387, 0.348–41.298, 0.033–10.877, and 0.808–86.482 µg/g, respectively. Quartz albums produced in Fengyang County, Anhui Province, had the lowest content of heavy metals. Correlation analysis showed that there was a strong correlation among the elements in the samples. Chemometrics were used to analyze the elemental content, and the results of the different models were found to be consistent and could be used to distinguish between Quartz albums of different geogenesis. Conclusion This study provided a reference for the quality control and safety of the clinical application of Quartz album.

自唐代开始,三棱便存在同名异物的现象,其中以莎草科植物荆三棱Scirpus yagara Ohwi和黑三棱科植物黑三棱Sparganium stoloniferum Buch.-Ham.为最多,前者的药用历史较长,后者为《中国药典》2020年版规定的三棱药材来源;历史上,三棱药材产区主要集中在黄河、长江及淮河流域的河南郑州、江苏南京、湖南岳阳等地,近年来,主产区转移至浙江南部的东阳、磐安、丽水等地;三棱一般于10月霜降后采收,削去外皮、晒干、醋炙用,基本延续了宋代的采收加工炮制方法.本文通过对历代本草文献的查阅、整理,结合实地调查,厘清三棱药材的品种和产地变迁,总结采收加工炮制方法,为三棱资源的研究和利用提供参考.

Euryales Semen (ES) is sought-after for thousand years due to its multiple properties, mainly from the two cultivars (i.e. South Gordon Euryale (SE) and North Gordon Euryale (wild type, WT)). Currently, no effective way was established to recognize the two valuable and analogous kernels. We found high analogies of macroscopical traits and discovered WT seed was affected by ambient temperature, showing ostensible significant relationships with latitude and sea-level pressure. LC-MS based untargeted metabolomics helped us identified 177 putative metabolites. Pathway analysis revealed the underlying and vital roles of flavonoids during seeds development. Our results strongly suggested a strong level of similarity of WT from various regions on the strength of metabolic data. A multivariable model containing 51 chemical markers satisfactorily categorized WT and SE. This study obtained could be used to guide the varietal discrimination of ES.

Nepeta tenuifolia is a medicinal plant rich in terpenoids and flavonoids with antiviral, immunoregulatory, and anti-inflammatory activities. The peltate glandular trichome (PGT) is a multicellular structure considered to be the primary storage organ for monoterpenes; it may serve as an ideal model for studying cell differentiation and the development of glandular trichomes (GTs). The genes that regulate the development of GTs have not yet been well studied. In this study, we identified NtMIXTA1, a GT development-associated gene from the R2R3 MYB SBG9 family. NtMIXTA1 overexpression in tobacco resulted in the production of longer and denser GTs. Virus-induced gene silencing of NtMIXTA1 resulted in lower PGT density, a significant reduction in monoterpene concentration, and the decreased expression of genes related to monoterpene biosynthesis. Comparative transcriptome and widely targeted metabolic analyses revealed that silencing NtMIXTA1 significantly influenced the expression of genes, and the production of metabolites involved in the biosynthesis of terpenoids, flavonoids, and lipids. This study provides a solid foundation describing a mechanism underlying the regulation of GT development. In addition, this study further deepens our understanding of the regulatory networks involved in GT development and GT development-associated metabolite flux, as well as provides valuable reference data for studying plants with a high medicinal value without genetic transformation.

目的:分析不同产地白石英样品中矿物成分及含量,评价不同产地白石英样品的质量,筛选白石英优质矿产资源,并建立白石英X射线衍射(XRD)Fourier特征指纹图谱.方法:通过偏光显微及XRD分析不同样品矿物成分及其含量,以结晶度和石英含量作为评价指标.采用粉末XRD对白石英样品进行分析,建立中药白石英XRD Fourier特征指纹图谱,并进行模糊聚类分析、主成分分析和相似性评价.结果:偏光显微分析结果显示,不同产地白石英样品在结晶度上有明显不同.物相分析结果显示白石英样品主要成分为石英和正长石,且大多数样品含有钠长石.不同产地白石英中石英质量分数为91.2％～99.4％,其中浙江省安吉县章村镇和安徽省绩溪县扬溪镇白石英中石英质量分数最高,均为99.4％,浙江安吉县杭垓镇产白石英中石英质量分数为91.2％.建立了以12个共有峰为特征指纹信息的白石英XRD Fourier指纹图谱分析方法,其XRD Fourier指纹图谱的相似度为0.993～1.000.结论:在所采集的样品中,白石英药材品质总体较好,石英质量分数均在90.0％以上,其中浙江省安吉县章村镇和安徽省绩溪县扬溪镇所产白石英品质最好,其次为福建永安小陶镇和江苏新沂阿湖镇产白石英.偏光显微特征观察和XRD Fourier指纹图谱分析法可用于白石英的鉴定与分析.

The aerial parts of Agastache rugosa are rich in essential oils containing monoterpenoids, phenylpropanoids, and aromatic compounds. These are used as herbs, perfume plants, and ornamental plants. Based on the difference in the constituents of the essential oil, A. rugosa is divided into pulegone and estragole chemotypes, but the mechanism of key metabolite biosynthesis in these two A. rugosa chemotypes remains unclear. In this study, we compared the morphological differences, metabolite constituents, and transcriptomic data between the two chemotypes of A. rugosa. Monoterpenoid was the main compound in the pulegone chemotype, and phenylpropanoid was the main compound in the estragole chemotype; however, limonene was detected in both chemotypes. Furthermore, 46 genes related to pulegone and estragole biosynthesis were identified. Limonene synthase, limonene-3-hydroxylase, and isopiperitenol dehydrogenase were upregulated in the pulegone chemotype, while phenylalanine ammonia-lyase, 4-coumarate: CoA ligase, CYP73A, coumaroyl-aldehyde dehydrogenase, and eugenol synthase were downregulated in the pulegone chemotype. We identified chavicol methyl transferase and limonene-3-hydroxylase in A. rugosa. This work not only provides the difference in morphology and metabolites in pulegone and estragole chemotypes, but also offers a molecular mechanism of volatile oil biosynthesis, which could be a basis for specialized metabolites in specialized chemotypes.

目的 研究黄芩茎叶总黄酮(SBTF)对结肠癌HCT116细胞凋亡、迁移和侵袭的影响.方法 采用CCK8法检测SBTF(5、10、20、50、100、200、400、600 μg·mL-1)对HCT116细胞增殖的影响;Annexin V/PI 双染法检测 SBTF(80、120、160μg·mL-1)对HCT116细胞凋亡的影响;JC-1染色法检测SBTF对细胞线粒体膜电位的影响;Transwell小室迁移和侵袭实验检测SBTF对细胞迁移和侵袭的影响;Western blotting法检测SBTF对细胞凋亡、迁移和侵袭相关蛋白表达的影响.结果 与对照组比较,SBTF可明显抑制HCT116细胞增殖,干预24、48 h后的半数抑制浓度(IC50)值分别为156.50、98.59 μg·mL-1;SBTF显著提高HCT116细胞凋亡率(P＜0.05、0.01);SBTF明显促进HCT116细胞线粒体膜电位改变;SBTF显著降低HCT116细胞迁移和侵袭率(P＜0.05、0.01);SBTF显著上调HCT116细胞的Bax/Bcl-2值和cleavedCaspase-3蛋白表达(P＜0.05、0.01),显著下调MMP-2、MMP-9蛋白表达(P＜0.05、0.01).结论 SBTF可通过诱导HCT116细胞凋亡和抑制细胞迁移、侵袭发挥抗结肠癌作用.

目的:研究黄芩汤对非酒精性脂肪肝炎(non-alcoholic steatohepatitis,NASH)的作用及机制.方法:将大鼠随机分为空白组、模型组、阳性对照组(多烯磷脂胆碱,8 mg·kg-1)、黄芩汤低、中、高剂量组(5,10,20 g·kg-1),采用高脂饮食饲喂12周造模.灌胃给药5周后,检测各组大鼠血脂、肝功能及炎性因子水平;HE染色观察肝脏组织病理变化;RT-PCR和Western Blot法检测肝组织Toll样受体2(TLR2)、髓样分化因子88(MyD88)、核因子κB p65(NF-κB p65)、白介素6(IL-6)、维甲酸相关核孤儿受体γt(RORγt)、IL-23和IL-17 mRNA和蛋白表达;免疫组化法检测肝组织中NF-κBp65表达.结果:与空白组比较,模型组大鼠血脂、肝功能和炎性因子水平明显异常(P＜0.01).肝组织出现脂肪变性及炎性浸润;肝组织中TLR2、MyD88、NF-κB p65、IL-6、RORγt、IL-23和IL-17 mRNA和蛋白表达明显升高(P＜0.01);NF-κB p65阳性细胞表达明显提升(P＜0.01).与模型组比较,黄芩汤组大鼠血脂、肝功能和炎性因子水平得到明显恢复(P＜0.05,P＜0.01);肝组织脂肪变性和炎性浸润得到纠正;上述蛋白mRNA和蛋白表达均明显降低(P＜0.05,P＜0.01);NF-κB p65阳性细胞表达明显降低(P＜0.01).结论:黄芩汤可能通过抑制TLR2/MyD88/NF-κB信号通路和IL-23/IL-17轴治疗NASH.

以培养新时代需求的中药鉴定学综合素质人才为目的,以成果产出为导向,以"金课"建设为标准,探讨构建中药鉴定学课程从理论到实践、从课内到课外、从线下到线上、从校内到校外、从分散到有机统一的多维立体"金课"教学模式.

目的 分析2种化学型黄花蒿萜类代谢物及其转录组数据信息,探讨2种化学型黄花蒿差异代谢物及其可能形成原因.方法 采集江苏盱眙县、河南中牟县两地黄花蒿种子于同一条件下种植,通过顶空-气相色谱-三重四极杆串联质谱(HS-GC-QQQ-MS/MS)分析黄花蒿的挥发性成分,超快速高效液相色谱-三重四级杆飞行时间串联质谱(UFLC-Triple TOF-MS/MS)非靶向分析2种化学型黄花蒿的代谢物,并采用转录组测序分析挥发性成分相关萜类生物合成基因的表达.结果 依据挥发性主成分类别分型,江苏盱眙县种源黄花蒿为蒿酮型,河南中牟县种源黄花蒿为樟脑型.UFLC-Triple TOF-MS/MS检测到的差异代谢物通过KEGG数据库分析可知蒿酮型和樟脑型黄花蒿分别在倍半萜、三萜生物合成和二萜生物合成路径显著上调.转录组数据中通向萜类骨架生物合成的甲基赤藓糖醇-4-磷酸(MEP)途径和甲羟戊酸(MVA)途径注释到11个关键酶的23个候选基因具有显著差异.单萜合成路径中,1,8-桉树脑合酶(TPS-Cin)共检测到10个候选基因.此外,还发现2个冰片脱氢酶候选基因在樟脑型黄花蒿中高表达,3个黄花蒿醇脱氢酶2(ADH2)候选基因在蒿酮型黄花蒿中高表达.结论 该研究为揭示黄花蒿化学型形成分子机制提供科学数据.

目的 建立测定玫瑰花氨基酸成分的超高效液相色谱-串联三重四极杆质谱法(ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry,UPLC-TQ/MS),为不同产地玫瑰花溯源和品质评价提供思路.方法 采用UPLC-TQ/MS结合多元统计学方法对7个产地50批玫瑰花样品中的20种氨基酸类成分进行定量分析与聚类分析(hierarchical clustering analysis,HCA)、主成分分析(principal componentsanalysis,PCA)和正交偏最小二乘法分析(orthogonal partial least squares discriminant analysis,OPLS-DA).结果 玫瑰花中含有丰富的氨基酸类成分,包括8种人体必需氨基酸,其中谷氨酸(glutamic acid,Glu)、天冬酰胺(asparagine,Asn)、酪氨酸(tyrosine,Tyr)、丝氨酸(serine,Ser)等含量较高,焦谷氨酸(pyroglutamic acid,Pyr)、亮氨酰-缬氨酸(leucoyl-valine,Leu-Val)、甲硫氨酸(methionine,Met)和 γ-氨基丁酸(γ-aminobutyric acid,GABA)含量较低.不同产地玫瑰花氨基酸含量差异较大.HCA、PCA和OPLS-DA结果显示,相同产地玫瑰花品质相似,不同产地品质差异较大.从氨基酸成分角度综合评价,四川双流和河北围场的玫瑰花品质较好.OPLS-DA结果进一步显示Asn、Glu、谷氨酰胺(glutamine,Gln)、Ser为不同产地玫瑰花的主要差异成分,提示不同产地诸多生态因子可能主要干预玫瑰花中Asn、Glu、Gln、Ser的形成与积累,从而造成品质差异.结论 本研究首次建立不同产地玫瑰花中氨基酸类成分的分析方法,结果可为玫瑰花产地溯源、质量控制与品质评价提供有益参考.