Ikan gabus (Channa sp.) merupakan ikan air tawar dengan kandungan protein dan albumin yang tinggi sehingga berpotensi dikembangkan sebagai pangan fungsional. Kukis adalah salah satu produk pangan yang digemari masyarakat namun umumnya memiliki kandungan protein dan asam lemak yang rendah sehingga diperlukan upaya fortifikasi untuk meningkatkan nilai gizinya. Penelitian ini bertujuan untuk menentukan pengaruh variasi penambahan enkapsulasi hidrolisat protein dan minyak ikan gabus terhadap karakteristik kukis. Penelitian menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan (penambahan enkapsulasi 0%, 3%, 6%, dan 9%) serta lima kali ulangan. Analisis meliputi uji fisik (colorimetri, texture analyzer, scanning electron microscopy), proksimat, dan hedonik. Data dianalisis secara statistik dan ditentukan perlakuan terbaik menggunakan metode De Garmo. Hasil menunjukkan bahwa penambahan enkapsulasi berpengaruh nyata terhadap sifat fisik, kimia, dan hedonik dari kukis. Penambahan enkapsulasi meningkatkan kadar protein, lemak, dan abu, namun menurunkan kadar karbohidrat serta kadar air kukis. Peningkatan penambahan enkapsulasi menyebabkan tekstur cookies lebih keras dan daya kembang menurun. Perlakuan terbaik diperoleh pada kue kering dengan enkapsulasi 6%, dengan kandungan protein, lemak, air, abu, dan karbohidrat masing-masing sebesar 7,15%, 3,78%, 3,10%, 1,62%, dan 84,35%. Berdasarkan hasil tersebut, karakteristik sensorik dapat diterima oleh panelis. Kesimpulannya, penambahan 6% minyak ikan dan enkapsulasi protein hidrolisat menghasilkan cookies dengan nilai gizi yang lebih baik dan sifat sensorik yang dapat diterima.

Acne vulgaris is a common inflammatory skin disorder largely associated with the proliferation of Propionibacterium acnes. Although antibiotics are widely used for acne treatment, the increasing incidence of antibiotic resistance necessitates the exploration of effective natural alternatives. Mangrove plants are known to contain diverse bioactive compounds with antimicrobial potential. This study aimed to evaluate the antibacterial activity of Natural Deep Eutectic Solvent (NADES) extracts derived from young and old leaves of Avicennia marina against P. acnes. Extraction was conducted using citric acid–glucose-based NADES with different molar ratios: P1 (1:1), P2 (2:1), P3 (3:1), and P4 (4:1), to assess the effect of solvent composition on antibacterial performance. Antibacterial activity was determined using inhibition zone assays, while molecular docking analysis was performed to investigate interactions between extracted metabolites and P. acnes target proteins. The results showed that both young and old leaf extracts exhibited significant antibacterial activity, strongly influenced by the NADES formulation. Young leaf extracts produced the largest inhibition zone with the P4 formulation (24.51±0.75 mm) and the smallest with P1 (21.63±0.67 mm). Similarly, old leaf extracts showed maximum inhibition in P4 (37.38±4.34 mm) and minimum inhibition in P1 (22.62±1.40 mm). Increasing the proportion of citric acid enhanced the extraction of bioactive compounds responsible for antibacterial activity. Molecular docking confirmed strong binding affinities between key metabolites and P. acnes target proteins, supporting the experimental findings. Thus, NADES extracts of A. marina leaves, particularly those prepared using a 4:1 citric acid–glucose ratio, demonstrate strong potential as natural antibacterial agents for acne prevention and treatment.

Abstract The anticipated increase in tilapia production is expected to result in a corresponding increase in fish waste, including components such as heads, skin, fins, tails, bones, viscera, and scales. If not adequately managed, this waste can pose significant environmental and health challenges. By 2020, hypertension is expected to become one of the leading causes of death worldwide. Therefore, there is a pressing need for technological solutions that can repurpose waste and address blood pressure concerns. One promising approach for utilizing fish waste is hydrolysis technology, which produces hydrolysate products with enhanced nutritional and functional properties, such as peptides. This study aimed to assess the potential of tilapia (Oreochromis niloticus) viscera hydrolysate peptides as nutraceutical antihypertensive agents using in silico methods. LCHR-MS analysis identified eight peptides: Gly-Leu (GL), Lys-Pro (KP), Val-Tyr (VY), Asp-Trp (NW), Gly-Pro (GP), Z-Leu-OH, Leu-Leu (LL), and Leucyl-leucyl-norleucine (LLX). These peptides demonstrated good bioavailability and were considered safe for consumption, with potential as antihypertensive and anti-inflammatory nutraceuticals, as determined through in silico analysis (SwissAdme, Pro Tox 3, Biopep). Moreover, molecular docking simulations indicated that the antihypertensive potential of the five peptides, ranked from highest to lowest, was as follows: Val-Tyr (VY), leucyl-leucyl-norleucine (LLX), Captopril, Lys-Pro (KP), Gly-Leu (GL), and Gly-Pro (GP).

Potensi dari aktivitas biologis pada kolagen dapat ditingkatkan salah satunya dengan cara hidrolisis enzimatis. Oleh karena itu, dilakukan pengujian hidrolisat kolegan kulit ikan gabus secara in vivo pada hewan model tikus wistar jantan dan selanjutnya dilakukan analisis kadar NO hewan model. Tujuan dari penelitian yaitu diharapkan dapat menganalisis adanya potensi dan pengaruh perbedaan level dosis pada hidrolisat kolagen kulit ikan gabus sebagai antihipertensi terhadap tekanan darah dan peningkatan kadar NO tikus wistar jantan hipertensi yang diinduksi NaCl dan Prednison. Metode penelitian ini menggunakan RAK dengan jumlah sampel 24 ekor dengan 6 level kelompok perlakuan, Kelompok Normal (N); Kelompok Kontrol Positif (K+); Kelompok Kontrol Negatif (K-); Kelompok Perlakuan Perbedaan Level Dosis Hidrolisat Kolagen kulit ikan gabus (P1,P2,P3). Penelitian terdiri dari dua tahap yakni tahap satu pembuatan dan identifikasi hidrolisat kolagen kulit ikan gabus, sedangkan tahap dua pengujian hidrolisat kolagen kulit ikan gabus melalui hewan model secara in vivo. Tahap 1 karakterisasi hidrolisat kolagen menggunakan enzim bromelin 1% diperoleh rendemen 12%, kadar protein 49,2mg/L, derajat hidrolisis 40,63%, berat molekul 15-180 kDa, dan memiliki 17 profil asam amino. Tahap 2, diperoleh hasil pengukuran tekanan darah hewan model meningkat menggunakan NaCl 2% + Prednison 1,5mg/kgBB dan menurun pada perlakuan berbeda tiap kelompok. Penelitian tahap 2 diperoleh kadar NO tertinggi oleh kelompok K+ 134,81 μmol/L, sedangkan kadar NO terendah oleh kelompok K- 38,67 μmol/L. Pemberian hidrolisat kolagen ikan gabus P3 (140 mg/200 g BB tikus) berpengaruh terhadap pengurangan kerusakan sel di area arteri pulmonalis jantung tikus wistar dan terjadinya dilatasi endotel jantung tikus wistar hingga sebesar 26 μm. The potential biological activity of collagen can be enhanced through enzymatic hydrolysis. Therefore, this study conducted in vivo testing of snakehead fish (Channa striata) skin collagen hydrolysate on male Wistar rats and subsequently analyzed nitric oxide (NO) levels in the animal models. The aim of the study was to assess the potential and impact of different dosage levels of snakehead fish skin collagen hydrolysate as an antihypertensive agent on blood pressure and NO levels in hypertensive male Wistar rats induced with NaCl + Prednisone. The research used a completely randomized design (CRD) with 24 samples divided into six treatment groups: Normal Group (N), Positive Control Group (K+), Negative Control Group (K-), and Treatment Groups with different dosage levels of snakehead fish skin collagen hydrolysate (P1, P2, P3). The study consisted of two stages: stage 1 involved the production and identification of snakehead fish skin collagen hydrolysate, while stage 2 involved in vivo testing of the hydrolysate on animal models. In stage 1, the collagen hydrolysate was characterized using 1% bromelain enzyme, resulting in a yield of 12%, protein content of 49.2 mg/L, degree of hydrolysis 40.63%, a molecular weight range of 15–180 kDa, and profiles of 17 amino acid. In stage 2, blood pressure measurements showed an increase in the animal model induced with 2% NaCl + 1.5 mg/kg BW Prednisone, which decreased with different treatments in each group. The results of stage 2 showed that the highest NO level was found in the K+ group (134.81 μmol/L), while the lowest NO level was observed in the K- group (38.67 μmol/L). Administration of snakehead fish collagen hydrolysate P3 (140 mg/200 g BW rats) significantly reduced cellular damage in the pulmonary artery area of the heart and caused endothelial dilation in the heart of Wistar rats, reaching 26 μm.

Desa Krenceng merupakan daerah yang banyak dialiri sungai yang digunakan untuk pengairan pertanian, tetapi juga berpotensi untuk budidaya perikanan. Hasil budidaya perikanan sebagian besar dijual segar dan masih sedikikit yang diolah menjadi produk turunan seperti nugget ikan, bakso ikan, abon ikan, dan lain-lain. Pada pengabdian kepada masyarakat (PKM) ini dipilih produk nugget ikan karena prosesnya mudah, dapat dikonsumsi semua golongan umur, dan berpotensi untuk mengatasi stunting. Tujuan pelaksanaan PKM ini untuk mengajarkan kepada mitra (kelompok Pemberdayaan Kesejahteraan Keluarga (PKK) Desa Krenceng, Kabupaten Blitar) membuat nugget ikan dan melakukan assessment mutu nugget ikan menggunakan uji organoleptik. Metode yang digunakan dalam PKM adalah Participatory Action Learning System (PALS). Mitra berpartisipasi dalam pembuatan nugget ikan dan penilaian mutu secara organoleptik melalui uji hedonik, skoring atribut, dan perbandingan jamak. Uji hedonik menilai tingkat kesukaan, skoring atribut mengukur intensitas sensori, sedangkan perbandingan jamak membandingkan karakteristik produk dengan produk sejenis di pasar. Mitra berhasil membuat produk nugget ikan nila. Hasilnya penilaian sensori hedonik diperoleh bahwa tingkat kesukaan atribut rasa, aroma, tekstur, dan kerenyahan nugget komersil masih lebih disukai daripada nugget ikan hasil praktek. Dari perbandingan jamak diperoleh bahwa atribut nugget ikan hasil praktek yang sama dengan nugget komersil hanya pada atribut warna, sedangkan atribut rasa, aroma, tekstur, dan kerenyahan berbeda dengan nugget komersil. Dengan demikian mitra mampu membuat produk nugget ikan dan mampu menilai mutu nugget ikan secara sensori, meskipun nugget hasil praktek masih lebih rendah mutunya daripada nugget komersil.

Inflammatory Bowel Disease (IBD) is a chronic gastrointestinal disorder driven by oxidative stress and immune dysregulation, often exacerbated by prolonged non-steroidal anti-inflammatory drug (NSAID) use. Conventional therapies provide symptomatic relief but are limited by severe adverse effects, necessitating safer alternatives. Channa striata (snakehead fish) is a rich source of albumin, essential amino acids, and bioactive compounds with potential immunomodulatory and anti-inflammatory effects. This study investigates the therapeutic efficacy of purified Channa striata albumin extract in an indomethacin-induced IBD rat model. Albumin was isolated via ammonium sulfate precipitation and characterized by SDS-PAGE, antioxidant activity (DPPH assay), and amino acid profiling. Rats were treated with Channa striata albumin (100-300 mg/kg BW) for 14 days post-indomethacin induction. Compared to controls and diclofenac-treated groups, the extract significantly reduced malondialdehyde (MDA) levels (up to 90.69%), improved jejunal histoarchitecture, and enhanced occludin expression. Immunohistochemistry showed a marked reduction in CD4+T cell infiltration, indicating immunomodulatory activity. These findings establish Channa striata albumin as a potent natural antioxidant and immunomodulator, offering a safer alternative to NSAIDs for IBD management.

One of the strategic plans of the Ministry of Maritime Affairs and Fisheries to increase the diversification of value-added fishery products is to utilize fishery products into ingredients that are beneficial for health. Snakehead fish is a product of freshwater fisheries that contains high albumin and has various health functions, including as an immunostimulant and anti-inflammatory. This research aims to determine the characteristics and potential of snakehead fish albumin extract as an immunostimulant and anti-inflammatory. Albumin extraction was carried out using the steaming method at 50°C for 6 hours. The analysis carried out included protein content, albumin content, amino acid profile, mineral content, and antioxidant activity. The results showed that snakehead fish extract had albumin levels of 36.15 mg/L, Fe mineral levels of 3.744 mg/L, Zn 1.553 mg/L, and Ca 50 mg/L. The amino acid profile shows that there are 15 amino acids consisting of 7 essential amino acids and 8 non-essential amino acids with the highest amino acid levels in glutamic acid, aspartic acid, lysine, leucine, arginine, and phenylalanine. The antioxidant activity of snakehead fish extract is classified as a moderate antioxidant with an IC50 amounting to 133.81 ppm.

Kulit ikan gabus (Channa striata) memiliki kandungan kolagen sekitar 13,60% yang dapat dimanfaatkan sebagai bahan baku produk nutraceutical. Hidrolisat kolagen kulit ikan gabus (Channa striata) diketahui memiliki aktivitas antihipertensi dengan aktivitas penghambatan Angiotensin Converting Enzyme (ACE) sebesar 78%. Penelitian dilaksanakan pada Bulan Maret hingga Mei 2024 di Laboratorium Advance Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, Laboratorium Perekayasaan Hasil Perikanan Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, dan Laboratorium Uji Hewan Farmakologi Universitas Brawijaya. Metode penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan jumlah sampel hewan model sebanyak 24 ekor dengan 6 level kelompok perlakuan yakni Kelompok Normal (N); Kelompok Kontrol Positif (K+); Kelompok Kontrol Negatif (K-); Kelompok Perlakuan Dosis Hidrolisat Kolagen Kulit Ikan (P1, P2, P3). Penelitian ini terdiri atas 2 tahap yakni tahap 1 dan tahap 2. Tahap 1 berupa pembuatan dan karakterisasi hidrolisat kolagen kulit ikan gabus (Channa striata) sedangkan tahap 2 berupa analisis aktivitas antihipertensi hidrolisat kolagen kulit ikan gabus (Channa striata) melalui hewan model secara in vivo. Pemberian hidrolisat kolagen kulit ikan gabus (Channa striata) dapat mencegah terjadinya peningkatan luas glomerulus ginjal hewan model serta mencegah terjadinya disfungsi endotel dan injury pada sel endotel dan podosit yang dapat mencegah terjadinya sklerotik glomerulus. Snakehead fish skin (Channa striata) contains about 13.60% collagen, which can be utilized as a raw material for nutraceutical products. Collagen hydrolysate from snakehead fish skin (Channa striata) has been shown to have antihypertensive activity, with an Angiotensin Converting Enzyme (ACE) inhibition activity of 78%. The research was conducted from March to May 2024 at the Advance Laboratory of the Faculty of Fisheries and Marine Science, Brawijaya University, the Fishery Product Engineering Laboratory of the Faculty of Fisheries and Marine Science, Brawijaya University, and the Pharmacological Animal Testing Laboratory of Brawijaya University. The research method used a Completely Randomized Design (CRD) with 24 animal model samples divided into 6 treatment groups: Normal Group (N), Positive Control Group (K+), Negative Control Group (K-), and Treatment Groups with Collagen Hydrolysate from Fish Skin (P1, P2, P3). This study consists of two stages: Stage 1 involves the preparation and characterization of collagen hydrolysate from snakehead fish skin (Channa striata), while Stage 2 involves analyzing the antihypertensive activity of the collagen hydrolysate from snakehead fish skin (Channa striata) in vivo using an animal model. The administration of collagen hydrolysate from snakehead fish skin (Channa striata) can prevent an increase in the glomerular area of the animal model’s kidneys, as well as prevent endothelial dysfunction and injury in endothelial and podocyte cells, which could help prevent glomerulosclerosis.

Quality feed in aquaculture is very important to increase the growth and health of fish. Low feed efficiency can be overcome by feed supplementation using catalytic enzymes. One of the enzymes that can stimulate digestion is protease in red ginger plants. The protease enzyme in red ginger plants plays a role in breaking peptide bonds in place of carboxyl groups so that peptides are formed from complex compounds into simpler compounds. This study aims to determine the profile of amino acids and proximate in feed given red ginger enzymes. This research uses descriptive method. The feed sample used was commercial feed with 4 treatments namely Control (without red ginger enzyme), treatment A (1%), treatment B (3%), and treatment C (5%). The analyzes carried out included red ginger enzyme extraction, protease enzyme purification, proximate and amino acid analysis of each type of feed. The results showed that the results of the SDS-Page analysis on the red ginger protease sample contained at 67.1 kDa. The results of the proximate analysis showed the highest value in feed containing 3% red ginger protease (B) with a percentage of 42.69%. The resulting amino acid content consists of 8 non-essential amino acids namely, L-serine, L-glutamic acid, L-alanine, L-arginine, glycine, L-aspartic acid, L-tyrosine and L-proline. While the essential amino acids consist of 7 types of amino acids namely, L-Phenylalani, L-Isoleucine, L-Valine, L-Lysine, L-Leucine, L-Threonine and L-Histidine. The best type of feed is feed B, which is feed with the addition of 3% red ginger protease.

Fish shredded is nutritious and has a soft texture, less stringy than shredded beef. The compounds in tuna shredded fortified with banana blossoms and their potential as antidiabetic are not yet known. The purpose of this study was to identify phytochemical bioactive compounds in tuna shredded fortified with banana blossoms and to study its potential as an antidiabetic functional food in silico. The 70% fortified tuna shreds were extracted using hexane, followed by ethyl acetate and finally using methanol. The methanol filtrate was evaporated to obtain methanol extract of shredded fish and analyzed using LC-HRMS. Phytochemical compounds were analyzed for antidiabetic potential in silico using Open Babel GUI software version 2.4.1, PyRx 0.8 (The scrips Research Institute), PyMOL™ 1.7.4.5, and Discovery Studio Visualizer (2021). The methanol extract of banana heart fortified tuna fish contained 32 compounds. The results of docking the compounds against the α-glucosidase receptor showed that the compound that had a lower or the same binding affinity with acarbose (-8.00 Kcal/mol) and native ligand (-6.00 Kcal/mol) was flurandrelonide (-9.40 kcal/mol), Quercetin-3β-D-glucoside (-9.30 kcal/mol), sakuranetin (-8.80 kcal/mol), curcumin (-7.70 kcal/mol), ar-turmerone (-7.20 kcal/mol), nootkatone (-7.20 kcal/mol), and ferulic acid (-6.90 kcal/mol). The lower the binding affinity value, the stronger the inhibition of α-glucosidase. Phytochemical compounds in shredded tuna fortified with banana blossoms that may act as antidiabetic agents through in silico α-glucosidase inhibition are flurandrelonide, quercetin-3β-glucosidase, sakuranetin, curcumin, ar-turmerone, and ferulic acid.

Tambelo reportedly has a positive impact on health. Our study investigates the possible effect of tambelo extract as an anti-inflammatory in mice induced by lipopolysaccharide(LPS)Tambelo phytochemistry from liquid chromatography analysis – high-resolution mass spectrometry (LCMS) coupled with NF-κB protein, docking was carried out to pursue anti-inflammatory properties. Thirty Balb/C mice were randomized into six groups: untreated, LPS, dexamethasone + LPS, and tambelo extract with doses of 100, 150, and 200 mg/kg BW + LPS, respectively. The results of this study indicate that there are four compounds which has a high affinity for the target protein. High CD4+CD25+ expression after treatment with fermented products tambelo (BE) significantly attenuated LPS-induced mice (p<0.05). Our findings show that fermentation tambelo protects against induced LPS by maintaining Tregs. Tambelo may have the potential as a food choice due to its anti-inflammatory properties.

Background and aim: Snakehead (Channa striata) contains protein 25.5% including albumin. C. striata contains approximately crude albumin 6.22% and the crude albumin extract has been used as wound healing supplement widely in Indonesia. Recently, the albumin extract has been produced in powder form. Albumin is classified as a globular protein, and the properties have been influenced by temperature and pH value. Therefore, coating material is needed to protect albumin, to stabilize the properties from protein denaturation and to improve the end-product quality. The aim of this study was to determine the effect of adding coating materials such as carboxymethyl cellulose (CMC), chitosan, alginate and pectin towards quality of crude albumin powder made from C. striata. Methods: The method used in this study was experimental method. CMC, chitosan, pectin and alginate have been added to crude albumin extract with five differences concentrations, respectively. The concentration of coating material were CMC 3%, chitosan 2%, alginate 2%, and pectin 1%. The parameters of this experiment were albumin content, protein content, moisture content, lipid content, ash content, water absorption and yield. Results: The results showed that albumin content was 8.09% (CMC), 7.29% (chitosan), 7.37% (alginate) and 6.73% (pectin). CMC 3% showed the highest albumin content among others coating materials. The nutritional value of albumin powder with additional CMC 3% was albumin content 8.08%, protein content 32.55%, moisture content 5.47%, ash content 3.30%, lipid content 4.91%, water absorption 2.37% and yield 5.99%. The lightness of the best treatment (CMC 3%) was 26.30 ±0.57. The amino acid analysis from the albumin powder coated by CMC 3% showed that this product contained amino acid are glutamic acid, aspartic acid, leucine. Moreover, the fatty acid profiles showed that the crude albumin powder contained oleic acid and dokosadienoic acid. In conclusion, additional CMC 3% towards crude albumin powder improved the quality of final product. Conclusions: Optimal snakehead fish albumin powder was obtained at a concentration of 3% CMC coating addition with qualities including albumin content of 8.08%, water content 5.47%, ash content 3.3%, protein content 32.55%, fat content 4.91 %, 2.37% water vapor absorption and 5.99% yield. In the brightness parameter, the value of L * 23.35 is obtained. The highest amino acid profiles are glutamic amino acids and aspartic amino acids. The highest fatty acid profiles are linoleic and lauric fatty acids.

Clarias sp. is one of the most common types of freshwater fish. The utilization of Clarias sp. is only limited to fresh consumption, whereas the nutritional content of Clarias sp. is potential as a source of nutrition. High protein and low-fat content make Clarias sp. can be processed into diversification of traditional food such as otak-otak products. The otak-otak products can be used as functional food, but there is a weakness i.e. the low of Calcium of otak-otak products. This study aims to determine (1) fortification of Moringa oleifera leaves on organoleptic and (2) mineral content of Calcium the otak-otak products. The method used is an experimental method with a simple Completely Randomized Design. Independent variables of this study were concentration of Moringa oleifera leaves powder 4%, 8% and 12% while dependent variable was Calcium content. The results showed that fortification of Moringa oleifera leaves powder had a significant effect on organoleptic aroma, taste, texture, appearance and Calcium content of the otak-otak products. The best fortification of Moringa oleifera leaves powder is 4% with the results of aroma of 41.00; taste 37.25; texture 38.00 and appearance of 38.05. Calcium content of otak-otak products reached 3.09±0.099 mg.

Ikan tuna mengandung gizi yang tinggi yaitu protein 22,6 – 26 gram/100 gram dan lemak 0,2- 2,7 gram/100 gram dengan proporsi 50-60% bagian tubuh dapat dikonsumsi. Dengan kandungan gizi ini, ikan tuna berpotensi diolah menjadi berbagai produk olahan salah satunya adalah abon ikan. Abon ikan memiliki daya simpan (shelf-life) yang relatif lama, yaitu selama 50 hari pada suhu kamar. Namun, abon ikan masih memiliki kekurangan yakni rendahnya kadar serat. Alternatif sumber serat yang dapat ditambahkan adalah jantung pisang. Penelitian ini menggunakan metode eksperimen Rancangan Acak Lengkap (RAL) dengan 3 taraf penambahan jantung pisang yaitu 20%, 25% dan 30% dengan 3 ulangan. Uji yang dilakukan adalah organoleptik untuk mengetahui tingkat kesuakaan panelis terhadap produk abon ikan. Uji organoleptik menggunakan 4 parameter yaitu penampakan, tektur, aroma dan rasa. Hasil perlakuan terbaik berdasarkan keempat parameter tersebut adalah konsentrasi penambahan jantung pisang sebanyak 25% dengan nilai kenampakan 4,067; aroma 3,956; tekstur 4,589; rasa 4,344 dan dengan penerimaan keseluruhan 4,389.  Kata kunci: Abon ikan, Jantung pisang, Tuna

Abstract. Akabessy JL, Yahya, Fadjar M, Suprayitno E. 2022. Identification of Tambelo (Bactronophorus thoracites) in Wamesa Waters, Manokwari, West Papua, Indonesia. Biodiversitas 23: 6021-6030. Tambelo, otherwise known as shipworm, is a marine bivalve mollusk comprising 16 genera in the Teridinidae family, of which Teredo is the most frequently mentioned. Furthermore, the only species in the genus Bactronophorus is the wood borer, Bactronophorus thoracites (Teredinidae), which has a wide distribution in the Indo-West Pacific biogeographical region. Identifying shipworm species is difficult due to their high diversity and the limited information about their taxonomic characteristics, which often leads to identification errors by collectors. Papuan people consume tambelo as a substitute for side dishes because they are believed to be able to cure various diseases. Therefore, this study aims to establish by morphology and molecular analysis that tambelo belongs to the B. thoracites of the Teredinidae family. Tambelo was identified using the morphological method of observing the physical characterization of the palette, which is unique between genera. It was collected from a mangrove forest near Wamesa Beach, South Manokwari, Manokwari Regency, Indonesia. Meanwhile, this study adopted PCR, an identification method that uses DNA from a small number of samples and can provide a DNA sample sequence. Morphological analysis based on pallet characterization showed that the characteristics of the tambelo pallet were similar to those of the B. thoracites based on the identification book. In addition, the molecular phylogenetic analysis using two mitochondrial protein-coding genes, cytochrome c oxidase subunit I (COI), showed tambelo was 91% similar to B. thoracites from mangroves environment on the west coast of Peninsular Malaysia, Malaysia.

Tuna ( Thunnus sp. ) is a high-economic fish that is most sought after in the Indonesian seas. Tuna has good nutrition for the human body, content such as high protein, vitamin and mineral content, and has a delicious taste, so this fish can be diversified. One of the products is fish floss that can be used as functional food. This study aims to determine: (1) determine effect of banana blossom addition in fish floss to organoleptic parameter (2) determine effect of banana blossom addition in fish floss to peroxide value and water content. The method used in this study is an experimental method with an experimental design in the form of a simple Completely Randomized Design (CRD). The independent variables in this study were the concentration of banana blossom 30%, 50%, and 70% while the dependent variables were organoleptic, water content and peroxide value. The best fortification of banana blossom is 70% with organoleptic score of appearance 5.178; scent 4.922; texture 5.078; and taste 4.944. The peroxide value in the tuna floss product with 70% banana blossom concentration is 0.578 meq/kg and water content 13.33%.

Cephalopod production from year to year has also increased. The purpose of this study was to compare the results of the proximate composition analysis and the characterization of the fatty acid content of squid (Loligo sp.) Obtained from Maluku waters. The analysis results showed that the proximate content in the form of the highest protein was in the waters of the Aru archipelago, followed by the regencies of West Southeast Maluku and West Seram (19.85%, 16.78%, 14.66%). The highest carbohydrates were in the samples from the waters of West Seram Regency then West Southeast Maluku and Aru Islands (7.3%, 3.3%, 2.09%). The highest fat content was dominated by samples from the waters of West Seram Regency, followed by West Southeast Maluku and Aru Islands (1.23%, 0.94%, 0.91%). The samples’ moisture collected was almost the same at the three locations, namely 76.58% in the waters of West Southeast Maluku Regency, 75.25% in West Seram, and 74.66% in the waters of Kepualauan Aru. The composition of ash and fiber was dominated by samples collected from the Aru Islands and Western Seram District (249% and 2.46%). The Fatty Acid test found that the results varied between locations, namely non-essential fatty acids, namely Palmitic Acid, which was dominated by location, and essential fatty acids, namely DHA, EPA, and ARA.

Background: Hypertension is a disease that has the potential to become the most significant cause of death in the world in 2020. Hypertension caused by an imbalance of the renin angiotensinogen system. Viscera hydrolyzate extract has an anti-hypertension activity. The research aimed to determine tilapia viscera hydrolyzate extract’s ability to reduce blood pressure, suppressing the expression of TNF-α and IL-6. Viscera hydrolyzed with alcalase and extracted to obtain viscera bioactive crude extract (VisBC).Methods: Spontaneously hypertensive rats induced by DOCA-salt, gave VisBC therapy. 15 Wistar rats divided into 5 treatment groups, namely normal control (without induced DOCA-salt), positive control (SHR-Spontaneous Hypertensive Rat = induced by DOCA-salt), comparative control (SHR + Captopril 5 mg/kg), VisBC 1 (SHR + BC 150 mg/kg) and VisBC 2 (SHR + BC 300 mg/kg), where each treatment consisted of 3 replications. Each treatment measured for blood pressure, TNF-α and IL-6 expression. The data represented as mean ± SD and analyzed by ANOVA (p less than 0.05) in any difference, a subsequent Duncan test. Data analyzed by SPSS 20.0 for Windows 10.Result: The results showed the treatment of VisBC could reduce systole (19.64%) and diastole (29.15%) blood pressure. Also, VisBC inhibited the expression of TNF-α (8.08%) and IL-6 (8.39%). The VisBC of tilapia with a dose of 150 mg/kg could inhibit the expression of TNF-α and IL-6, thereby affected the decrease in blood pressure (systole and diastole) in rats induced by DOCA-salt. This research proved that fish waste could produce bioactive as anti-hypertension.

Efforts in pioneering lobster cultivation require information on the maintenance of spiny lobsters (Panulirus versicolor), especially the specific feed doses required for their growth.This study aims to obtain information about the ideal feed dose for spiny lobster (Panulirus versicolor) growth and survival.The samples included spiny lobsters (Panulirus versicolor), which were obtained from the fishermen's catch and lands on Osi Island, West Seram Regency, Maluku Province.Each tank consisted of 8 samples with an initial weight between 600-650 gr.This research was conducted for six months, and utilized specific doses of feed as a treatment: A: 3%, B: 5%, C: 7% of the tested lobster's bodyweight.Furthermore, the physical boundaries measured include Specific Growth Rate (%), absolute growth, and water quality maintenance.The results showed that lobsters fed with a dose of 5% had a significant weight gain compared to the 3% and 7% doses.Therefore, the SGR was higher for lobsters fed at a dose of 5% (5.83%), followed by the ones treated with 7% (5.64%) and 3% (4.42%).In comparison, for the absolute growth average, the best treatment dose was 5% (75.82gr), followed by that of 3% (69.97gr) and 7% (57.55 gr).From the results, it can be concluded that a feed dose of 5% of body weight provides a relatively high growth rate compared to other treatments.Therefore, the optimal feed dose for the cultivation of spiny lobster (Panulirus versicolor) is 5%.

AbstractAlbumin powder is an alternative development of snakehead fish albumin extract which is useful for wound healing besides albumin powder also reduces fishy taste and is easy to use. One way is to process the powder into using a vacuum drying method with a temperature of 49o. Wound healing is also influenced by several factors other than albumin. Among other things, the role of omega3, omega-6, and collagen. The results of the first stage of the study showed that different concentrations of grapeseed oil affected the quality of albumin powder including: omega-6 levels, yield, protein, fat, water, and ash. The best albumin powder is using omega 6 5% concentration with the results: omega-6 content 2.89%, yield 59.18%, protein 4.03%, fat 7.69%, water 2.49%, ash 1, 01%. The results of the second stage of the study showed that the highest fatty acid was linoleic acid (2.88%). Observation of wound closure in mice showed that 5% omega-6 concentration was the best with 70% wound closure. Day 7