Safety evaluation data have been presented for a shampoo formulation containing zinc 2-pyridinethiol 1-oxide at 2% and 10% levels. In addition toxicity studies have been carried out in dogs with a w o emulsion of ZnPT. The dog seems to be the species most susceptible to the effects of the compound itself, but it shows no effects to the shampoo formulation. The Rodentia show paralytic symptoms when the material is ingested in the diet at rather low levels for a period of several weeks. No ocular toxicity is observed in rabbits, rats, or monkeys, but it is observed in the dog. The shampoo containing ZnPT did not produce sensitization or undue irritation of the skin. In addition the effect of the shampoo vehicle on rabbit eyes was not increased by the incorporation of ZnPT. Since the shampoo formulation investigated is emetic and does not penetrate the skin, there would appear to be no hazard associated with its use.

Five brighteners used in household detergent products have been investigated from the standpoints of acute oral toxicity, subacute percutaneous toxicity, skin tumorigenicity, and sensitization of the skin. LD50 values ranged from 2.8 g/kg to greater than 21.5 g/kg in mouse, rat, guinea pig, and rabbit. No toxicity resulted from the daily topical application of 1.5 mg/kg of each brightener to the skin of rabbits for 90 days. None of the tested materials induced tumors in 2-year skin paintings on mice. None of the brighteners were sensitizers.

The acute and chronic toxicity of sodium lauryl glyceryl ether sulfonate and sodium lauryl trioxyethylene sulfate have been studied in rats, and skin tumorigenicity has been investigated in mice. LD50 values determined by oral administration to rats were 1.82 g/kg for both compounds. Rats received the surface-active agents at levels of 0.1% and 0.5% in the diet for two years, with some animals sacrificed for examination at the end of one year. No adverse effects were observed in the experimental animals with respect to survival, growth, reproduction, food consumption, hematologic values, blood chemistry, or urine analyses. Microscopic evaluation of tissues revealed no pathogenic changes resulting from ingestion of the test materials. Scattered differences were found in organ: body weight ratios. No effects of the surfactants were observed in first or second generation offspring of the original animals. Twice-weekly application, for 105 weeks, of 5% aqueous solutions of sodium lauryl glyceryl ether sulfonate and sodium lauryl trioxyethylene sulfate to the skin of Swiss female mice did not lead to the development of skin tumors in any of the animals. Based on the results of these investigations there is no evidence that exposure to these two surfactants poses a toxic hazard to humans.

Experiments in rats and dogs, employing various dosage regimes, demonstrate urinary elimination accounts for roughly only 10 to 30 per cent of administered Decapryn Succinate. The antihistamine does not accumulate in various body tissues, and it is concluded that the bulk of administered Decapryn Succinate is destroyed in the body.