Strain ZY1909104T was isolated from the nasal cavity of a pig with respiratory illness in Yunnan Province, south-west China, and characterized to determine its taxonomic status. The strain was Gram-stain-positive, oxidase- and catalase-negative, non-motile, facultative anaerobic coccus arranged in pairs. The strain was capable of growth at temperature range of 22–43 °C (optimum 37 °C), at pH level 6.0–9.0 (optimum pH 7.0) and in the presence of 0–1.5

Bovine rotavirus (BRV) poses a major threat to the global cattle industry, driving significant morbidity and mortality in young calves. In Yunnan Province, China, BRV is the primary cause of neonatal calf diarrhea (NCD), yet the molecular epidemiology of circulating strains remains poorly understood. This study aimed to investigate the molecular characteristics of bovine rotavirus strains associated with a severe outbreak of the NCD on a local farm. Fecal samples were collected from 396 calves and screened for BRV by RT-PCR targeting the VP6 gene. Positive samples were subjected to virus isolation in MA104 cells, followed by whole-genome sequencing, phylogenetic analysis, and pathogenicity assessment in suckling mice. Of 396 samples, 85 tested positive for BRV, corresponding to an animal-level prevalence of 21.5% (95% CI: 17.5-25.8%), with four fatalities recorded. A strain designated as BRV-YN1-2021 was successfully isolated, exhibiting characteristic cytopathic effects, specific immunofluorescence, and typical rotavirus morphology by electron microscopy. Genomic analysis revealed the constellation G8-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3, identified as genotype G8P[1]. BLAST analysis showed that four genomic segments shared the highest identity with deer rotavirus strains, five with human rotavirus strains, and two with bovine rotavirus strains. Phylogenetic analysis demonstrated close relationships with US deer strains, Japanese bovine strains, and human strains circulating in China. Experimental infection in suckling mice induced diarrhea and significant intestinal histopathology, degeneration of villous epithelial cells, goblet cell hyperplasia, and inflammatory infiltration. This study reports the first isolation of a G8P[1] bovine rotavirus from a diarrhea outbreak in Chinese cattle. The multi-host genetic composition provides evidence of interspecies reassortment events, highlighting the zoonotic potential of BRV and emphasizing the need for continuous molecular surveillance to inform effective control strategies.

In China, the Akabane virus (AKAVs) has been reported in several host species. However, data regarding goats is still showing a gap. Akabane virus (AKAV) is an insect-borne virus from the Peribunyaviridae family that in ruminant species, particularly affects pregnant animals, resulting in abortions, stillbirths, and premature broth, often with congenital abnormalities. Therefore, there is a dire need to understand the diversity of this virus in ruminants, particularly in goats. The current study aimed to investigate the genotype characteristics of goat-originated AKAVs in Yunnan, China. For this, blood samples from goats were collected for four consecutive years (2019-2023) during routine disease surveillance in Yunnan province. The serum was harvested and evaluated for the seroprevalence of the AKAVs. The seroprevalence analysis revealed that the majority of goats in Yunnan province are infected with AKAVs, with a detected prevalence of 7.69% (92/1,197 tested), even though detected seroprevalence rose as high as 27.8% in some areas. For the evolutionary analysis of AKAVs of goat, five AKAVs strains were isolated from AKAV serum-positive goat blood samples were whole genome sequenced. The phylogenetic analysis (sequenced small and medium segments) classified the AKAVs into Ia and Ib genogroups, with Ia genogroup strains being more common in Yunnan goats. However, genotype II (TJ2016 and CQ-AKAV-1-2023) emerged in China. Overall, this study emphasizes the importance of implementing prevention and control strategies for AKAVs diseases transmitted in Yunnan, China.

A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820(T), was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0-9.0 (optimum pH 7.0), at 24-39 degrees C (optimum 37 degrees C) and with 0.5-2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to Acinetobacter kanungonis JCM 34131(T). Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (Acinetobacter calcoaceticus DSM 30006(T)), 37.0% (Fluviicoccus keumensis JCM 19370(T)), and 64.0% (Acinetobacter kanungonis JCM 34131(T)), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C-12:0, C-16:0, summed feature 3 (C-16:1 omega 7c and/ or C-16:1 omega 6c), C-17:0, and C-15:1 omega 6c as the major fatty acid (> 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820(T) represents a novel species of a new genus in the family Moraxellaceae, and the name Nasibacterium caprae gen. nov., sp. nov. is proposed. The type strain is ZY210820(T) (= CCTCC AB 2021475(T) = NBRC 115474(T)).

Group A rotaviruses (RVA) have been identified as the leading cause of neonatal calf diarrhea (NCD) In Yunnan, China, despite the limited documentation on this issue within the country. Given the significant impact on animal health and welfare, further attention is warranted to comprehend the prevalence and epidemiology of this disease. In this study, we isolated RVA strains from NCD clinical samples to support RVA epidemic study and further disease control in Yunnan. Material and Methods: Ten fecal samples were obtained from calves with a single episode of neonate calf diarrhea from a farmer during a severe NCD outbreak. For the diagnosis of Group A rotavirus (RVA) infection, fecal reverse transcription-polymerase chain reaction (RT-PCR) was employed as the primary method. Subsequently, RVA isolation was conducted on MA104 cells, with inoculates being pretreated with trypsin TPCK to enhance viral infectivity. The isolated RVA strain was further confirmed through RT-PCR and immunofluorescence assay. Results: RVA virus was isolated from clinical samples following six passages depended on trypsin treatment of inoculates. The sign of CPE detected consisted of increased cell granularity, obscure cell boundaries, cell rounding, and eventual degeneration and detachment of cells. Analysis of sequencing revealed that field isolates was of group A serotype. Phylogenetic analysis based on the ORF sequences of the VP4 and VP7 sequences segments indicated that the isolate was classified as the P[1] genotype. VP4 gene of strain YN-1 shares 75.2%-99.7% nucleotide identity of and 81.6%–99.7% amino acid identity with the complete VP4 sequences of the P[1]. The complete VP7 gene of YN-1 is 981 nucleotide long and encode 326 amino acids. The RotaC v2.0 analysis classified the sequence as the G8 genotype. The VP7 gene of strain YN-1 shares 88.5%-97.7% nucleotide identity and 94.2%-99.1% amino acid identity. Conclusion: This is the first study in China which reports the circulation of Group A NCD on cattle farms in the field. Our study constitutes a crucial and a necessary step allowing preventive and veterinary medicine to support RVA disease controls in Yunnan province, China.

Abstract The primary focus of this investigation was the meticulous examination of the genetic composition and protein structure of the B2L and F1L genes. Notably, specimens were meticulously procured from the oral cavities of infected goats. Subsequent to verification through polymerase chain reaction (PCR), the virus was successfully cultured in goat testis cells. The designated ORFV strains were denoted as ORFV/goat/YNSLi/China/2021/Yunnan,ORFV/goat/YNSLi/China/2023/Yunnan,ORFV/goat/YNYLn/China/2022/Yunnan,and ORFV/goat/YNTJe/China/2023/Yunnan. The genetic data pertaining to the core genes (B2L, F1L) were meticulously scrutinized to elucidate their epidemiological and evolutionary attributes. Analysis of the B2L gene sequences revealed that all four strains were classified within cluster I of group I, with discernible variations in sequences across the temporal spectrum. Conversely, inspection of the F1L gene sequences unveiled that YNSLi/2023 and YNTJe/2023 were positioned in cluster I of group I, YNSLi/2021 resided in cluster III of group I, and YNYLn/2022 was allocated to cluster IV. The distinctive Yunnan ORFV strains exhibited noteworthy characteristics, including gene rearrangements during the outbreak. Examination of the protein structures showcased variances in hydrophobicity and stability in comparison to other strains. While similarities were noted in hydrophilic flexibility, antigenic index, and surface accessibility, discrepancies in amino acids were observed to influence protein structure. Projections derived from the analysis of the B2L and F1L genes suggested the absence of signal peptides and transmembrane domains. This comprehensive study contributes significantly to the comprehension of ORFV transmission and evolution, thereby furnishing invaluable insights for the formulation of preventive and control strategies within Yunnan Province.

Contagious Eczema (CE), caused by ORFV, impacts sheep and goats globally, with severe symptoms and economic losses. The ORFV situation in Yunnan, China, was unclear before 2021-2023 study. Eleven scab samples from goats on small farms in three Yunnan municipalities were collected. Four ORFV strains were isolated and characterized using scanning electron microscopy, cytopathic effect observation, and PCR. Phylogenetic analyses of ORFV011 and ORFV059 genes showed significant results. For ORFV011, the nucleotide similarity of the four strains to D1701 strain was 98.4-99%. For ORFV059, it was 97.2-97.9% with OV-SA00 strain. These findings suggest gene rearrangements and interactions among strains during Yunnan's ORFV outbreak, forming a unique evolutionary lineage. Our study is the first comprehensive one on Yunnan's ORFV prevalence with in-depth phylogenetic analysis. It has important implications. In vaccine development, understanding genetic variances helps create better vaccines. For disease control, customized strategies like targeted quarantine and disinfection can be designed based on strain characteristics. From a public health aspect, as CE is zoonotic, closely monitoring ORFV in goats aids in predicting and preventing human infections, thus being significant for protecting goats against CE in Yunnan.

2018 年云南省边境地区某猪场猪群出现高热等症状,部分猪只死亡.为确认发病原因,采集死亡猪只肺脏组织样品进行猪繁殖与呼吸综合征病毒(PRRSV)RT-PCR检测,然后将阳性样品接种Marc-145 细胞,连续传代3 次后发现产生稳定的细胞病变效应(CPE).经间接免疫荧光试验(IFA)、毒价测定及病毒全基因组测序,最终确定病原为PRRSV,将其命名为YNML-2018 株,毒价测定为 10-4.88 TCID50/0.1 mL.全基因组序列分析显示,YNML-2018 毒株基因组全长 15 357 bp,包含 8 个开放阅读框(ORF),其中非结构蛋白 2 编码基因(NSP2)缺失 90 个碱基.病毒全基因组遗传进化分析显示,该毒株序列与国内分离的高致病性PRRSV同源性为 95.3%～99.3%;其NSP2 高变区序列与国内分离的高致病性PRRSV同源性为 92.1%～97.8%;结构蛋白GP3、GP5氨基酸序列与国内分离的高致病性PRRSV毒株同源性分别为84.3%～99.6%和83.1%～99.0%.结果表明,YNML-2018 株与近年来我国PRRSV流行株相比存在一定变异,但变异程度较低,仍与首次报道的高致病性毒株JXA1 同属于亚群V.本研究为掌握云南省边境地区PRRSV流行毒株的遗传变异特征提供了技术支撑,并可为疫苗选用提供数据参考.

A novel Gram-stain-negative, aerobic, irregular coccus designated as ZY201224T, was isolated from the nasal cavity of a goat with respiratory disease in a goat farm, located at Jianshui, Yunnan Province, PR China and its taxonomic position was clarified using a polyphasic approach. The strain grew optimally at 37 °C, at pH 8.0 and in the presence of 1

Abstract Rotavirus is classified within the family Reoviridae, subfamily Sedoreovirinae, and the genus Rotavirus. This genus encompasses eight groups, denoted as species A to H, with Group A holding particular significance due to its widespread prevalence among both human and animal populations. Among these, Bovine rotavirus (BoRV) stands out as the leading cause of substantial morbidity, mortality, and economic losses in neonatal calves.Rotavirus is classified within the family Reoviridae, subfamily Sedoreovirinae, and the genus Rotavirus. This genus encompasses eight groups, denoted as species A to H, with Group A holding particular significance due to its widespread prevalence among both human and animal populations. Among these, Bovine rotavirus (BoRV) stands out as the leading cause of substantial morbidity, mortality, and economic losses in neonatal calves. In order to identify the etiology of cattle with diarrheal diseases and further enrich the epidemiological data of bovine rotavirus (BRV) in China, RT-PCR was used to identify virus pathogens from 10 diarrheal cattle fecal samples. Positive samples were inoculated in MA-104 cells, generating stable cytopathologies from the sixth generation. Basic physicochemical properties, structural functions and functions of the VP4/VP7 proteins of the virus were predicted and analyzed by indirect immunofluorescence test (IFA). The results identified the isolated virus was BRV and it was named RVA/Cattle/CHN/YN1/2021/G8P. Following RT-PCR amplification, sequencing and splicing of 11 gene segments of this strain, homology and typing analyses were conducted. A genetic tree of isolated strains was constructed based on VP7 and VP4 sequences, and the genetic evolutionary relationship and the variation of amino acid sequences were analyzed. The results showed that among 11 fragments of the YN-1 genome, 5 segments showed high nucleotide identity with a deer strain, 2 segments with human, 1 with U.S. vaccine strain; 7 segments highly identified with a U.S. strain, among them 5 segments were highly identified with strain C/Cervidae/United States/14-02218-2/2014. Bioinformatics analysis results indicated that both VP4 and VP7 proteins are stable, hydrophilic, transmembrane and non-secretory proteins. The number of O-glycosylation, N-glycosylation, phosphorylation sites were 150 and 59, 3, 102 and 34 in VP4 and VP7, respectively. Subcellular localization is located in the plasma membrane, with α-Spiral and extension as the main structures, with irregular curls and β-The corner running through it. Therefore, the genome constellation of this strain was determined as G8-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H. The study results lay a foundational work for the development of vaccines, and detection kits, which also support the effective prevention and control of BRV infection in China in the near future.

In order to understand the pathogenic bacteria species causing subcutaneous abscess,and its distribution characteristics in goats in Yunnan Province,bacterial isolation and identificationand PCR were used to detect 128 subcutaneous abscess samples collected from goatsin goat farms in Xishan District of Kunming City,Shilin County,Yiliang County and Mile County,and the results of the two methods were compared.Among the 128 subcutaneous abscess samples,the PCR detec-tion rates of Arcanobacterium pyogenes,Corynebacterium pseudotuberculosis and S.aureus were 17.19％(22/128),20.31％(26/128)and 67.19％(86/128),respectively.The isolation rates of A.pyogenes,C.pseudotuberculosis,S.aureus and Streptococcus ovis were 9.38％(12/128),20.31％(26/128),65.63％(84/128)and 1.56％(2/128),respectively.Among the 128 subcutaneous abscess samples,the detection rates of single bacterial species in the bacterial isolation and identification method and the PCR method were 95.31％(122/128)and 92.19％(118/128),respectively.The total coincidence rate of the bacterial isolation and identification method and PCR method was 90.63％(116/128).The results showed that S.aureus was the most common pathogenic bacteria species causing subcutaneous abscess in goats in Yunnan Province.Moreover,S.ovis strains were i-solated from goat subcutaneous abscess samples for the first time,suggesting that S.ovis may be a potential new pathogen causing subcutaneous abscess in goats.This study provides basis for the prevention and control of subcutaneous abscess in goats in Yunnan Province.

为了对云南1起山羊皮下脓肿进行细菌学诊断,本研究从皮下脓肿的脓液样品中分离到2株革兰阳性链状球菌YN220769和YN220770,对2株分离株进行形态学、生理生化和16S rDNA基因鉴定并分析其药物敏感性.细菌鉴定结果显示2分离株生化特征与加勒多尼亚链球菌(Streptococcus caledonicus)模式菌株CCUG 73951T一致;与S.caledonicus参考株之间的同源性为99.6％～100.0％,与S.caledonicus CCUG 73951T之间的同源性分别为99.7％和99.9％;与5株S.caledonicus参考株形成同一个进化分支;根据鉴定结果,将YN220769和YN220770鉴定为S.caledonicus.致病性试验显示2株分离菌对小鼠有致病性.药敏试验结果显示2株分离菌对青霉素、阿莫西林、头孢噻吩等多种抗生物敏感,对磺胺甲恶唑耐药.本研究首次从山羊皮下脓肿分离到S.caledonicus,证实山羊S.caledonicus感染的存在,对山羊S.caledonicus感染的预防和控制提供了理论依据.

为制备一种阿卡斑病毒灭活疫苗并初步评价其免疫效果,本研究应用组织半数感染量(TCID50)测定的方法对阿卡斑病毒CX-01 株在HmLu-1、Vero、BHK-21 细胞上的扩繁规律进行判定,研究病毒在终浓度 0.002 mol/L 的BEI灭活剂、37℃条件下的灭活规律,然后应用ISA 206 佐剂乳化制备疫苗,通过小鼠模型评估抗原中的添加剂蔗糖、海藻糖和左旋咪唑对免疫效果的影响.结果:HmLu-1 细胞培养病毒效价最高为106.75 TCID50/mL,优于Vero和BHK-21 细胞;终浓度为0.002 mol/L的BEI灭活剂处理5h即可完全灭活病毒,为确保对阿卡斑病毒的灭活完全彻底,在BEI灭活剂用量和灭活条件不变的前提下,选择最优的灭活时间为8h;添加 5%蔗糖、5%海藻糖、1%左旋咪唑的疫苗组免疫效果最好,平均中和抗体滴度为18.0.本研究结果对阿卡斑病毒的培养和灭活疫苗的制备具有一定的借鉴意义.

Strain ZY190618T, isolated from the nasal cavity of a cow with respiratory disease, was subjected to taxonomic characterization. Cells of the strain were Gram-stain-negative, aerobic and coccus-shaped. Phylogenetic analysis based on 16 S rRNA gene sequences indicated that the strain belonged to the genus Moraxella with the highest similarity of 98.1% to Moraxella nasovis CCUG 75922T. Phylogenomic analysis based on 810 single-copy genes revealed that the strain was a member of the genus Moraxella and formed a deep and separated clade within the genus. The strain showed the highest orthologous average nucleotide identity (OrthoANI) value of 77.1% with Moraxella ovis CCUG 354T and digital DNA-DNA hybridization (dDDH) value of 24.7% with Moraxella equi NCTC 11012T, respectively. The DNA G + C content was 46.5 mol%. The strain optimally grew at 37 °C (temperature range, 24–42 °C), at pH 8.0 (pH range, 6.0–9.0) and with 1.5% (w/v) NaCl (NaCl range, 0.5–3.0%). The strain contained C18:1 ω9c as the sole predominant fatty acid (> 5 %) and CoQ-8 as the major respiratory quinone. The major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin and hemibismonoacylglycerophosphate. Based on these data, strain ZY190618T clearly represents a novel species in the genus Moraxella, for which the name Moraxella nasibovis sp. nov. (The type strain ZY190618T = CCUG 75921T = CCTCC AB 2021472T) is proposed.

Abstract Introduction Akabane virus (AKAV) has been detected in a variety of host species in China, but there are only limited records of its occurrence in goats. However, more attention needs to be paid to understanding the diversity of viruses in this species. The aim of the study was to explore the genotype characteristics and variation trend of AKAV and their relationship with virulence in Yunnan, China. Material and Methods Blood samples were collected from goats during routine surveillance of goat diseases in Yunnan province in 2019. The AKAV CX-01 strain was isolated using BHK-21 cells. To understand pathogenicity, the virus was intraperitoneally (IP) and intracerebrally (IC) inoculated into suckling mice and tissue samples were subsequently analysed histopathologically and immunohistochemically. Results Akabane virus CX-01 strain induced encephalitis and impairment of the central nervous system with fatal consequences. Phylogenetic analysis based on the ORF sequences of the small segments indicated that the AKAV isolate used was most closely related to the GD18134/2018 Chinese midge and bovine NM BS/1strains, while phylogenetic analysis based on the medium segments showed a close relationship between CX-01 and the Chinese GLXCH01 strain. Conclusion The CX-01 isolate was related to AKAV genogroup Ia and probably originated from a recombination of different strains.

为了对1起羊腹泻病例进行诊断,从腹泻病羊粪便中分离到10株革兰阳性棒状杆菌YN21083～YN210812,对分离株进行形态学和16SrDNA基因鉴定并研究其药物敏感性和耐药基因特征.NCBI数据库BLAST比对鉴定结果显示,10株分离株与无枝菌酸棒状杆菌(Corynebacterium amycolatum)德国分离株FDAARGOS_991的同源性为99.3％～100.0％.16S rDNA基因进化分析显示10株分离株与C.amycolatum参考株形成同一个进化分支,与C.amycolatum参考株之间的同源性为97.2％～100.0％,与C.amycolatum模式菌株ATCC49368T之间的同源性为98.3％～99.2％,将10株分离株鉴定为C.amycolatum.药敏试验结果显示,10株分离菌株对青霉素、氨苄西林、阿莫西林克拉维酸、头孢噻吩、头孢呋辛、氟苯尼考、阿米卡星和四环素敏感,对卡那霉素和磺胺甲恶唑耐药.耐药基因PCR检测结果显示,共检测到8种耐药基因aadA1、aadA 2、tetA、tetM、qnrS、Sul1、Sul2和Sul3,其检出率分别为30.0％,30.0％,100.0％,60.0％,60.0％,100.0％,10.0％和 20.0％.本研究首次报道羊 C.amycolatum 感染,对羊C.amycolatum感染的预防和控制具有重要指导意义.

为分析口蹄疫病毒(foot-and-mouth disease virus,FMDV)减毒株感染天然宿主细胞后基因表达变化,将O型FMDV兔化弱毒株YNTBa接种初生犊牛原代睾丸细胞(BT),采用二代测序技术对YNTBa感染BT细胞后8 h和24 h的细胞转录组进行研究.结果显示:病毒感染8 h和24h的试验组较对照组分别筛选出437和134个表达变化≥2倍的差异表达基因.基因本体论(GO)功能类聚分析显示,差异表达基因主要参与到细胞分化、DNA转录、细胞因子产生和凋亡等.京都基因与基因组百科全书(KEGG)功能富聚分析表明,差异表达基因主要与细胞代谢、核糖体、吞噬体、蛋白消化吸收、细胞分化、细胞转录调控等相关,涉及糖基化终末产物受体(AGE-RAGE)、肿瘤坏死因子(TNF)、白细胞介素17(IL-17)、转化生长因子β(TGF-β)、磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K-Akt)、丝裂原活化蛋白激酶(MAPK)、促性腺激素释放激素(GnRH)、雌性激素(estrogen)和催产素(oxytocin)等多条信号通路.利用实时荧光定量PCR(RT-qPCR)验证了其中12个差异表达基因,定量结果与转录组测序结果一致.本研究筛选的差异表达基因为进一步了解FMDV弱毒免疫机制,包括FMDV复制相关基因提供了基础.

A novel Gram-stain-negative, aerobic, coccus-shaped bacteria, designated ZY201115T, was isolated from the nasal cavity of a sheep with respiratory disease in Yunnan Province, south-west China, and its taxonomic affiliation was studied by applying a polyphasic approach. The strain grew at 18-41 °C (optimum, 37 °C), at pH 6.0-9.0 (optimum, pH 8.0) and in 0.5-3.0% (w/v) NaCl (optimum, 1.0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain is affiliated to the genus Moraxella with highest similarity to Moraxella bovis ATCC 10900T (96.6 %). Phylogenomic analysis based on 811 single-copy genes also indicated that the strain represents a novel species in the genus Moraxella and formed a deep and separated clade with Moraxella caviae NCTC 10293T. The highest genomic orthologous average nucleotide identity and digital DNA-DNA hybridization values between the strain and the type strains in the genus Moraxella were 73.7% (M. caviae NCTC 10293T) and 25.3% (Moraxella osloensis CCUG 350T), respectively. The G+C content of the complete genome sequence was 42.1 mol%. The predominant fatty acids (>5 %) were C18:1 ω9c, C17:1 ω8c, C12:03OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The major polar lipids were phosphatidylglycerol, cardiolipin, monolysocardiolipin, phosphatidylethanolamine and hemibismonoacylglycerophosphate. The major respiratory quinone was CoQ-8. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic characterizations, strain ZY201115T clearly represents a novel species of the genus Moraxella, for which the name Moraxella nasovis sp. nov. is proposed. The type strain is ZY201115T (=CCTCC AB 2021473T=CCUG 75922T).

为探究2020年冬季云南省昆明市某山羊场持续发生的羊呼吸道疾病病因,从该场采集病料,经样品DNA提取、临床症状及病理剖检观察、病原分离纯化和16S rRNA PCR扩增鉴定,确定病原为绵羊肺炎支原体,并将分离株命名为YN-2020.随后设计引物,扩增YN-2020株延伸因子(TU)和热休克蛋白基因(HSP70)全长序列,分别与GenBank中相关参考菌株构建遗传进化树开展生物信息学分析,并对TU及HSP70基因进行体外原核表达.生物信息学分析结果显示:YN-2020分离株TU基因与绵羊肺炎支原体菌株MoGH3-3亲缘关系较近,在不同支原体种间,其与牛殊异支原体亲缘关系较近,与禽滑液囊支原体亲缘关系较远;HSP70基因与绵羊肺炎支原体Movi 117株亲缘关系较近,在不同支原体种间,其与牛殊异支原体亲缘关系较近,与猪鼻支原体亲缘关系较远.TU及HSP70基因原核表达结果显示,两种蛋白均能在大肠杆菌表达系统中获得不同程度的分泌性表达,TU重组蛋白大小约48 kDa,HSP70重组蛋白大小约68 kDa,且能与康复期绵羊血清发生反应.本研究可为进一步认识绵羊肺炎支原体提供参考,并在此基础上完善相关诊断方法的研究.

为了解云南省畜禽细菌耐药基因分布特征,采用PCR方法检测94株畜禽细菌中β-内酰胺类、四环素类、磺胺类、大环内酯类、喹诺酮类、氨基糖苷类、磺胺类和氯霉素类共23种耐药基因.结果 显示,共检测到23种耐药基因,其中aacA4(90.43％)基因检出率最高,其次是floR(87.23％)、tetA (80.85％)、qnrS (77.66％)、qnrD(74.47％)、tetM(71.28％)、sul2(69.15％)、aadA1(53.19％)、blaTEM(45.74％)、sul1(40.43％)和sul2(38.30％).携带8种及8种以上耐药基因的菌株比例为73.40％,其中携带耐药基因数最多(14种)的菌株比例为2.13％.地域分布上,保山市畜禽细菌耐药基因的种类(20.00种)和每株细菌平均携带耐药基因数量(11.24种)均高于红河州、昆明市、普洱市和楚雄州;畜禽品种分布上,鸡细菌耐药基因的种类(22.00种)和每株细菌平均携带耐药基因数量(9.11种)均高于猪、牛和羊.本研究结果表明,aacA4、floR、tetA、qnrS、qnrD、tetM、sul2、aadA1和bla TEM等多种耐药基因广泛分布于云南省畜禽细菌,不同地区、不同种类畜禽和不同种类细菌耐药基因的分布存在差异.本研究结果对云南省畜禽养殖场兽用抗生素的科学合理应用具有重要的指导意义.