Objective To study the role of DNA methylation in oral leukoplakia carcinogenesis. Methods DNA methylation was detected in forty cases of oral squamous cell carcinoma (OSCC), twenty-eight cases of oral leukoplakia (OLK) and forty cases of healthy oral mucosa. Download the expression profile data of OSCC, OLK and healthy oral mucosa from Gene Expression Omnibus (GEO) database. DNA methylation data and expression profile data were compared for repeatability, DNA methylation data for difference analysis and corresponding expression profile data for IPA pathway analysis. Results The data analysis showed that DNA methylation had greater flexibility and instability. Ingenuity Pathway Analysis (IPA) analysis showed that genes related to OLK differential methylation sites were mainly concentrated in the process of cell movement and differentiation. Genes related to differential methylation sites of OSCC are mainly enriched in cell proliferation, migration, oxidation regulation, and anti-apoptosis processes. The genes associated with OLK and OSCC differential methylation sites are co-enriched in phosphoinositol metabolism and phospholipase C signaling pathway. Conclusion DNA methylation is involved in the formation of oral squamous cell carcinoma, and the activation of phosphoinositol metabolism may promote oral leukinoma.

目的:明确D-半乳糖对正常人口腔黏膜细胞(normalhuman oral keratinocytes,NHOKs)促衰老作用,并探索潜在机制.方法:体外分离培养NHOKs,角形蛋白和波形蛋白免疫细胞化学染色法鉴定NHOKs.分别以10、20、40、80g/LD-半乳糖处理诱导细胞作为实验组,正常培养液组作为对照组.通过CCK-8法检测细胞增殖活力,采用衰老相关β半乳糖苷酶染色(senescence-associated-β-galactosidase,SA-β-gal)分析细胞衰老情况,Western blot检测衰老相关蛋白p53和p21表达水平.活性氧(reactive oxygen species,ROS)检测试剂盒检测细胞内氧化应激水平,Western blot检测氧化应激相关蛋白sirtl的表达.结果:D-半乳糖抑制NHOK细胞增殖,具有时间和浓度依赖性.D-半乳糖增加SA-p-gal染色阳性率,上调衰老相关基因p53和p21,显著升高细胞内ROS,降低氧化应激相关蛋白sirtl表达.结论:D-半乳糖可诱导NHOKs发生衰老,该过程可能与细胞氧化应激有关.

目的:研究鼠衰老过程中口腔颊黏膜上皮厚度的改变,为口腔黏膜衰老模型的后期建立提供自然衰老数据.方法:常规饲养雄性Wistar大鼠至3月龄、12月龄和24月龄,分别作为自然衰老青年、中年和老年组,对其进行口腔颊黏膜取材,石蜡包埋、切片、苏木精伊红染色,在显微镜下对其上皮厚度进行测量和分析.结果:Wistar大鼠的口腔颊黏膜上皮角化层的厚度和基底层至粒层的厚度在老年组中显著高于青年组或中年组,青年组和中年组相比无显著差异.结论:Wistar大鼠自然衰老过程中口腔颊黏膜上皮厚度增加,但因样本量小,该结论有待进一步实验证实.

Objective:To evaluate bleeding status of oral surgery with National Nosocomial Infection Surveillance risk index of 0 (NNIS 0) in patients on oral anticoagulated aspirin therapy using meta-analysis system.Methods:The PubMed and Wanfang databases were searched.Eligible studies were randomized controlled trials (RCTs) and controlled clinical trials on postoperative hemorrhage of continuation of aspirin before oral NNIS 0 surgery published from January 1,2007 to December 31,2016.Results:A total of 98 articles were retrieved and five studies were included in meta-analysis.The results showed no significant difference between patients on aspirin therapy and no oral anticoagulated therapy when postoperative bleeding were observed at 30 minutes (OR=1.23,95％CI:0.90-1.68,p=0.221).Conclusions:We recommend not stopping use aspirin (≤ 150mg/d) before oral NNIS 0 surgery.

Assessment and evaluation of oral health-related quality of life(OHRQL) can objectively reflect the physical and psychological effect of oral diseases on patients.We reviewed current concepts of quality of life,various forms of health survey,and clinical applications.And we summarized definitions and contents of generic quality of life,OHRQL,OHRQL questionnaire and available clinical applications.

检索Pubmed数据库,收集2006～2010年间关于复发性阿弗他溃疡(RAU)治疗的文献;依据循证医学评价方法对其进行分析。评价近五年内对于RAU治疗方案的有效性及其研究质量。