INTRODUCTION:Periodontitis, a chronic inflammatory disease that severely compromises oral health and is linked to systemic disorders, has attracted increasing research attention. Kangfuxin (KFX), an ethanol extract derived from Periplaneta americana (L.) with documented anti-inflammatory and tissue-regenerative properties, remains unexplored for localized periodontal therapy. This study investigated whether KFX extended-release gel (KFX-ERG) could inhibit the progression of periodontal disease and provide potential support for the treatment of periodontitis. MATERIAL AND METHODS:We selected the optimal concentration from different component ratios of KFX-ERG for subsequent experiments. In vivo, a periodontitis model was established in rats, and pathological changes in periodontal tissue were examined using histological and micro-computed tomography (micro-CT) analyses. RESULTS:Micro-computed tomography revealed that KFX-ERG reduced alveolar bone resorption in experimental periodontitis in rats. Histopathological analysis further revealed markedly reduced inflammatory cell infiltration and concurrently elevated osteoblast density in periodontal tissues of the KFX-ERG group. CONCLUSIONS:This study establishes a theoretical foundation for the therapeutic application of KFX-ERG in mitigating periodontitis progression and demonstrates its significant potential to promote periodontal tissue regeneration, thereby offering promising translational prospects for clinical regenerative periodontal therapy.

The sensitive and accurate detection of copper ions is crucial for public health, medical research, and environmental monitoring. In this study, we developed a sensor based on template-assembly activation of the primer exchange reaction (PER) for the on-site detection of copper ions in blood. Copper ions triggered the assembly of two template fragments into a hairpin structure via a click-chemistry reaction, activating the PER. Polymerase then repeatedly guided the polymerization of multiple primers along the assembled PER template, generating amplified products containing G-triplex sequences. These G-triplex structures specifically enhanced the fluorescence of thioflavin T and exhibited peroxidase-like activity, serving as dual-functional signal reporters for both fluorescence and colorimetric outputs. The sensor effectively amplified a single copper ion signal into multiple G-triplex signals, achieving high sensitivity with detection limits of 68.5 nM in fluorescence mode and 70.4 nM in colorimetric mode. The system also demonstrated strong selectivity, due to the high specificity of the click-chemistry reaction for copper ions. The dual-mode detection minimized false signals and improved accuracy through cross-verification. Additionally, both fluorescence and colorimetric signals were easily measurable without complex instrumentation, enabling flexible application. The method was successfully applied to blood samples, showcasing its robust performance. This sensor offers a promising alternative for copper ion detection with broad potential applications in public health, medical research, and environmental monitoring.

The commercial value and diversified applications of donkey hide gelatin (DHG) have precipitated sophisticated adulteration practices, particularly with camel-hide gelatin substitutes. We present a mass spectrometry-based strategy to authenticate species-specific collagen peptides in commercial DHG. A new qualitative and quantitative methodology is constructed for camel hide gelatin(CHG) material analysis in DHG. It consists of enzyme-mediated digestion, ultra-high-performance liquid chromatography (UHPLC), and triple quadrupole mass spectrometry (MS/MS) with multireaction monitoring (MRM) mode. In brief, the samples were digested with trypsin and subjected to efficient and quick separation by UHPLC, and MS/MS performed CHG identification and determination analysis. Two peptides (Camel peptide A, CPA, and Camel peptide B, CPB) were identified as CHG-specific peptides. A specificity test was then used to verify these two peptides. The method performance showed that these two peptides could distinguish CHG from other animal hide gelatins. The CPA and CPB detection limits were 5 μg/kg and 3 μg/kg, respectively. LC-MS/MS analysis detected CPA and CPB in 4.5% of market-collected DHG samples (n = 157), confirming the prevalence of this emerging adulteration practice. Thus, the present protocol is a sensitive, accurate, quick, and suitable application of species-specific peptide biomarkers, ensuring the quality of DHG products and making them authentic and traceable to protect consumers from potential health risks and food frauds.

The invasion and metastasis of tumors pose significant challenges in the treatment of ovarian cancer (OC), making it difficult to cure. One potential treatment approach that has gained attention is the use of matrix metalloproteinase reactive controlled release micelle preparations. In this study, we developed a novel PEG5000-PVGLIG-hyaluronic acid docetaxel/bakuchiol (PP-HA-DTX/BAK) micelles formulation with desirable characteristics such as particle size, narrow polydispersity index, and a ZETA potential of approximately -5 mV. The surface modification with HA facilitates tumor penetration into the tumor interior, while the incorporation of DSPE-PEG2000-PVGLIG-PEG5000helps conceal DSPE-PEG2000-HA, reducing off-target effects and prolonging drug circulation timein vivo. Bothin vitroandin vivoexperiments demonstrated that these micelles effectively inhibit proliferation, invasion, and metastasis of OC cells while promoting apoptosis. Therefore, our findings suggest that PP-HA-DTX/BAK micelles represent a safe and effective therapeutic strategy for treating OC.

INTRODUCTION: Periodontitis is a serious gum infection that disrupts the soft tissue around teeth. This study aimed to identify the most effective fraction of the Chinese medicine Kangfuxin for periodontitis treatment in a rat model. MATERIAL AND METHODS: Kangfuxin solution was subjected to sequential extraction using chloroform, ethyl acetate, n-butanol, and water. The extracts were evaporated, dissolved in DMSO, diluted in water, and administered to rats via gavage (0.5 mL/day) for 2 weeks. The n-butanol extract was further fractionated using macroporous resin chromatography with 10%, 30%, 50%, 70%, and 90% ethanol elution. Levels of inflammatory cytokines IL-6, IL-1β, and TNF-α in periodontitis samples were examined by ELISA. Leukocyte infiltration in the cementum was analysed by haematoxylin and eosin (H&E) staining. RESULTS: The n-butanol extract showed the best anti-inflammatory effect, reducing IL-6, IL-1β, and TNF-α levels in periodontitis samples and alleviating tissue damage and leukocyte infiltration in the cementum. Further fractionation revealed that the 50% ethanol fraction of the n-butanol extract had the most potent action in attenuating inflammation. This fraction suppressed the activation of the PI3K-AKT-mTOR signalling pathway in periodontitis samples. Application of a PI3K activator counteracted the anti-inflammatory effect of the 50% ethanol fraction. CONCLUSIONS: We identified a potent anti-inflammatory fraction (50% ethanol fraction of the n-butanol extract) of Kangfuxin for periodontitis treatment. This fraction suppressed the activity of the PI3K-AKT-mTOR signalling pathway in periodontitis samples. Further research is needed to isolate and characterise the specific bioactive compounds within this fraction.

The rapid advancement of photodynamic therapy (PDT) antibacterial materials has led to promising alternatives to antibiotics for treating bacterial infections. However, antibacterial drugs have poor light absorption and utilization rates, which limits their practical application. Constructing two-dimensional (2D) heterojunctions from materials with matching photophysical properties has emerged as a highly effective strategy for achieving high-efficiency photo-antibacterial performance. Here, we designed and prepared an atom co-sharing Bi/Bi4O5Br2 nanosheet heterojunction by a simple in situ reduction. This heterojunction material combines outstanding biocompatibility with excellent bactericidal efficiency, which exceeded 90% against Escherichia coli (a Gram-negative bacterium) and Staphylococcus aureus (a Gram-positive bacterium) under visible light irradiation, around nine-fold higher than that with pure Bi4O5Br2 nanosheets. The results suggest that localized surface plasmon resonance (LSPR) of shared Bi atoms on the Bi4O5Br2 nanosheets promotes light utilization and the separation and transfer of photo-generated charges, thus producing more abundant reactive oxygen species (ROS), which can partake in the PDT antibacterial effect. Our study underscores the potential utility of LSPR-enhanced Bi-based nanosheet heterojunctions for safe and efficient PDT to combat bacterial infections.

为了准确测定黄精中稀土元素的含量,采用微波消解法处理黄精样品后,通过在线引入内标溶液,用电感耦合等离子体质谱(ICP-MS)法同时测定黄精中15种稀土元素(La、Ce、Pr、Nd、Pm、Sm、Eu、Gd、Tb、Dy、Ho、Er、Tm、Yb、Lu)的含量,15种稀土元素的线性关系、精密度良好,平均加标回收率在97.0％～103％,相对标准偏差不大于1.7％.采用OriginPro 2021绘制稀土元素指纹图谱和进行主成分分析,以稀土元素含量的平均值构建黄精稀土元素标准指纹图谱,作为鉴定黄精中药材的参考依据;采用SPSS 26.0进行聚类分析.聚类分析结果表明,当平方欧式距离为10时,浙江产地样品(S5～S8)聚为一类,其余的聚为一类,与主成分分析结果一致.黄精药材中15种稀土元素的指纹图谱具有相似的分布形态,具有较强的特征性和一致性,相似度均大于0.950.黄精中Ce、La、Nd含量相对较高,其中Ce含量最高为53.0～2005μg/kg.方法操作简单,准确可靠,能满足实验分析要求,可为黄精的质量控制和药理研究提供参考,建立的指纹图谱可用于黄精的鉴别.

提出了超高效液相色谱-三重四极杆质谱法(UHPLC-MS/MS)同时测定化妆品中13种α-羟基酸含量的方法.样品0.2 g经水超声提取30 min(不易分散样品经异丙醇分散均匀后用水超声提取),提取液经离心过滤后采用UHPLC-MS/MS分析,13种 目标物在 Agilent Zorbax RRHD SB-Aq C18色谱柱上分离,以不同体积比的0.1％(体积分数)甲酸溶液和乙腈混合液为流动相进行梯度洗脱,质谱分析采用电喷雾离子源,负离子、多反应监测模式采集.结果表明:13种α-羟基酸的质量浓度在一定范围内与对应的峰面积呈线性关系,羟基辛酸、二苯乙醇酸、α-羟基癸酸的检出限(3S/N)为0.030 μg·g-1,羟基乙酸、乳酸的检出限(3S/N)为3.0 μg·g-1,其余8种α-羟基酸的检出限均为0.30 μg·g-1.按照标准加入法进行回收试验,回收率为85.1％～114％,测定值的相对标准偏差(n=6)均小于10％.方法用于分析20批样品,α-羟基酸检出总量为20～6 800 mg/100 g,仅有1批α-羟基酸总量超标.

目的 考察不同产地红参中铁(Fe)、锰(Mn)、锌(Zn)、铜(Cu)、硒(Se)、铅(Pb)、砷(As)、汞(Hg)和镉(Cd)9 种无机元素的含量差异.方法 采用微波消解技术及电感耦合等离子体质谱(ICP-MS)法测定 28 批不同产地红参中 9 种无机元素含量,并采用SPSS 22.0 软件进行聚类分析.结果 不同产地红参Fe、Mn、Zn含量均较高,Se含量极低,有害元素Pb、As、Hg、Cd、Cu含量均符合《中国药典》限量标准.28 批样品聚类为两类.结论 不同产地红参均富含Fe、Mn、Zn,其重金属及有害元素污染程度小,安全性相对较好.不同产地红参Fe、Zn、Cu、Se、As元素含量差异性均较小,Mn、Pb、Hg、Cd元素差异性较大.

目的 建立沙棘籽油软胶囊中 4 种生育酚的高效液相色谱(HPLC)含量测定方法.方法 采用Agilent Poroshell 120 PFP色谱柱(4.6 mm×250 mm,4 μm),流动相为甲醇-水(94 ∶ 6),检测波长为 294 nm,柱温为 26℃,流速为 0.9 mL·min-1.结果 α-生育酚、β-生育酚、γ-生育酚和δ-生育酚分别在1.83～91.48、1.86～93.09、1.84～91.85、1.77～88.70 μg·mL-1范围内呈良好的线性关系,平均回收率在96.32%～98.45%之间,RSD在0.48%～1.43%.结论 建立的方法重复性好,专属性强,能够用于测定沙棘籽油软胶囊中 4 种生育酚的含量.

目的 建立多波长高效液相色谱-二极管阵列检测器(HPLC-DAD)法同时测定美白类化妆品中 8 种甘草化学成分的含量.方法 样品经 70%甲醇溶液提取、过滤后,经Phenomenex C18色谱柱(4.6 mm×250 mm,5 μm)分离,流动相为乙腈-0.1%磷酸,梯度洗脱,231、249 和 365 nm波长同时检测,流速 1.0 mL?min-1,柱温 30℃.结果8 种成分分离度良好,在各进样浓度范围内线性关系良好,相关系数r均大于 0.998;4 种不同化妆品基质的平均加样回收率(n=3)为 88.3%～96.2%,相对标准偏差(RSD)为 0.9%～3.5%.结论 本方法准确可靠、灵敏度高,可用于同时测定化妆品中 8 种甘草化学成分.

为了解爽身粉类化妆品的质量安全现状,采用《化妆品安全技术规范》(2015年版)中相应的检测方法对市售753批爽身粉类化妆品进行硼酸、硼酸盐和重金属元素铅的检测,以期发现风险点并提出合理的监管建议.结果共发现不合格样品4批,不合格率为0.53％,均为重金属元素铅超标.针对检验结果进行多维度比较分析,提出针对爽身粉类化妆品的合理化监管建议.

建立了高效液相色谱法测定化妆品原料羟丙基四氢吡喃三醇的含量及构型比例的方法.样品以水溶解,经CAPCELL PAK ADME色谱柱分离,示差折光检测器测定,柱温为35℃,检测池温度为40℃,流速为1.0 mL/min.结果显示,羟丙基四氢吡喃三醇在0.1～2.0 mg/mL范围内线性关系良好,相关系数(r)≥0.9999,加标回收率为99.3％～99.6％,相对标准偏差(n=3)小于0.76％,方法检出限和定量限分别为0.03％和0.10％.该方法具有操作简单、定量准确、能够有效分离异构体等优点,适用于化妆品原料羟丙基四氢吡喃三醇含量及构型比例的测定.

采用氨基酸衍生物反应试验(ADRA)对 20 种化学防晒剂的致敏性进行评价.利用半胱氨酸衍生物N-[2-(1-萘基)乙酰基]-L-半胱氨酸(NAC)和赖氨酸衍生物α-N-[2-(1-萘基)乙酰基]-L-赖氨酸(NAL)模拟皮肤蛋白,与化学防晒剂共同反应,通过高效液相色谱法测定 20 种化学防晒剂的氨基酸衍生物消耗百分比,并对皮肤致敏性进行预测.通过比较ADRA预测结果与欧盟消费者安全科学委员会(SCCS)出示的化合物评估报告的一致性,判断方法的可行性.结果表明,3-亚苄基樟脑等 19 种化学防晒剂预测结果为阴性,二苯酮-3 预测结果为阳性.ADRA 可作为动物试验体外替代方法,简便、快捷地对化合物的皮肤致敏性进行预测和筛选.

PURPOSE:Our pilot study showed that a 3-dimensional dual drug delivery scaffold (DDDS) loaded with Chinese herbs significantly increased the regenerated bone volume fraction. This study aimed to confirm the synergistic anti-inflammatory and osteogenic preclinical effects of this system.METHODS:The targets and pathways of parthenolide and naringin were predicted. Three cell models were used to assess the anti-inflammatory effects of parthenolide and the osteogenic effects of naringin. First, the distance between the cementoenamel junction and alveolar bone crest (CEJ-ABC) and the bone mineral density (BMD) of surgical defects were measured in a rat model of periodontitis with periodontal fenestration defects. Additionally, the mRNA expression levels of matrix metallopeptidase 9 (MMP9) and alkaline phosphatase (ALP) were measured. Furthermore, the number of inflammatory cells and osteoclasts, as well as the protein expression levels of tumor necrosis factor-alpha (TNF-α) and levels of ALP were determined.RESULTS:Target prediction suggested prostaglandin peroxidase synthase (PTGS2) as a potential target of parthenolide, while cytochrome P450 family 19 subfamily A1 (CYP19A1) and taste 2 receptor member 31 (TAS2R31) were potential targets of naringin. Parthenolide mainly targeted inflammation-related pathways, while naringin participated in steroid hormone synthesis and taste transduction. In vitro experiments revealed significant anti-inflammatory effects of parthenolide on RAW264.7 cells, and significant osteogenic effects of naringin on bone marrow mesenchymal stem cells and MC3T3-E1 cells. DDDS loaded with parthenolide and naringin decreased the CEJ-ABC distance and increased BMD and ALP levels in a time-dependent manner. Inflammation was significantly alleviated after 14 days of DDDS treatment. Additionally, after 56 days, the DDDS group exhibited the highest BMD and ALP levels.CONCLUSIONS:DDDS loaded with parthenolide and naringin in a rat model achieved significant synergistic anti-inflammatory and osteogenic effects, providing powerful preclinical evidence.

建立了一种电感耦合等离子体发射光谱法(ICP-OES)测定化妆品原料羟丙基四氢吡喃三醇中硼酸盐残留量的方法.采用硝酸-过氧化氢-微波消解法进行样品消解,ICP-OES法测定,标准曲线法定量.结果显示,在0.5～20.0μg·mL-1范围内线性关系良好,相关系数为0.9995,回收率在98.3％ ～100.6％,相对标准偏差(RSD)在1.7％ ～2.9％,硼酸盐的检出浓度、定量浓度(以硼酸计)分别为0.6、1.8 mg/kg,重复性检测结果的RSD为1.4％(n=6).该方法操作简单,重复性良好,结果准确,适用于化妆品原料羟丙基四氢吡喃三醇中硼酸盐残留量的测定.

在0.50 g牙膏样品中加入90％(体积分数)甲醇溶液5 mL,涡旋1 min后加水稀释至25.0 mL,超声提取20 min,离心5 min.上清液过Cleanert IC-RP小柱,弃去前4 mL流出液,后续流出液供离子排阻色谱分析.以3 mmol·L-1甲烷磺酸和60 mmol·L-1甘露醇的混合溶液为淋洗液,以25 mmol·L-1四甲基氢氧化铵和15 mmol·L-1甘露醇的混合溶液为再生液,以IonPac ICE Borate色谱柱分离,用抑制型电导检测器检测.结果显示:硼酸质量浓度在1.0～100.0 mg·L-1内与其对应的峰面积呈线性关系,检出限(3S/N)为0.002％;方法用于实际样品的分析,测定值的相对标准偏差(n=6)为1.9％,加标回收率为98.3％～105％,检出量均未超过GB 22115-2008规定的限值.

目的:建立采用离子排斥色谱法测定药用软膏及乳膏中硼酸及硼砂含量的方法.方法:样品经90％甲醇溶液分散提取,Cleanert IC-RP小柱净化后,以3.0 mmol·L-1甲烷磺酸+60 mmol·甘L-1露醇的溶液为淋洗液,25 mmol·L-1四甲基氢氧化铵+15 mmol·L-1甘露醇的溶液为再生液,经IonPac ICE Borate离子排斥分析柱(250 mm x9 mm)分离,抑制型电导器检测器检测.结果:硼酸质量浓度在1～200 mg·L-1范围内线性关系良好,相关系数为0.999 6;检测限为0.5 mg·L-1,定量限为1.0 mg·L-1;软膏剂3个水平的加样回收率(n=3)在99.5％～99.7％,RSD在0.9％～1.3％,乳膏剂3个水平的加样回收率(n=3)在99.2％～100.2％,RSD在1.1％～1.7％.按本法对10批软膏及乳膏中的硼酸进行测定,其中5批硼酸软膏的含量为4.89％～5.21％,5批丁硼乳膏的含量为2.39％～2.68％.结论:该方法适用于软膏及乳膏中硼酸及硼砂的含量测定.

为了解防晒类化妆品中防晒剂的使用情况,采用高效液相色谱法(HPLC)对来自不同厂家的116批防晒类化妆品中使用的23种化学防晒剂进行测定,并对防晒剂的种类、频率和用量统计分析,比对检出情况与标签标识和批件的一致性,探讨市售防晒类化妆品的潜在风险点.结果表明116批防晒产品多以几种防晒剂复配使用,同时使用4种者最多;共检出18种防晒剂,其中,甲氧基肉桂酸乙基己酯使用频率最高,为86.2％;未发现防晒剂超量使用情况;13批样品实际检出成分与批件或标识成分不一致;在纳米原料和其他化学防晒剂的使用、标签标识与批件管理、产品功效与标识的一致性、复配防晒剂的使用和流通环节均可能存在风险隐患.因此,应认真对待上述风险,以保护消费者的健康,为防晒化妆品的科学监管提供参考.

目的 研究不同产地黄精中15 种元素的含量,建立黄精无机元素特征图谱,并对其质量进行综合评价.方法 样品经微波消解后,采用电感耦合等离子体质谱法测定,并采用OriginPro 2021 和SPSS 26.0 对数据进行皮尔逊相关性分析、主成分分析和聚类分析.结果 26 批黄精样品中Fe、Mn、Zn、Sr 含量相对较高,其中Fe含量为23.4～583.8 mg/kg;Mn含量为8.2～258.8 mg/kg;Zn含量为7.5～81.4 mg/kg;Sr含量为10.0～47.8 mg/kg;5 种重金属元素Pb、Cd、As、Hg、Cu 均未超标,Cu 的含量相对较高,平均含量为3.8 mg/kg,5 种重金属元素含量整体变化趋势具有一致性.结论 黄精药材中各元素具有相似的分布形态,具有特征性.主成分分析筛选出Cr、Fe、V、Cd、Mn、Sr 是黄精的特征无机元素.26 批黄精药材的质量差异相对较大,样品中得分最高、质量最好的是浙江江山的黄精(S5～S8),得分比较低的是四川和贵州的黄精药材(S9～S18).聚类分析结果与黄精的产地一致.该研究方法可为黄精的质量控制和鉴别工作提供技术支持和参考.