A surface plasmon resonance (SPR) biosensor inhibition immunoassay for determination of ractopamine (Rac) residue in pork was constructed by immobilising Rac derivative on the SPR-2004 biosensor chip. After extraction with perchloric acid, pork sample was cleaned by ethyl acetate and analyzed by SPR-2004 biosensor. The limit of detection (LOD) was 0.6μg/kg for pork sample. Recoveries of Rac were higher than 80% with relative standard deviations below 10%. Although the same pretreatment was applied for both the UPLC–MS/MS and the biosensor, the biosensor showed little matrix interference by constructing pure solution and matrix-match calibration curves. Compared with Biacore Q made by Biacore AB, SPR-2004 biosensor made by Chinese Academy of Sciences which is of low cost and showed minimal matrix interferences. Accordingly, this biosensor was a promising screening instrument to be used for detection of ractopamine in supermarkets, food factories and food regulatory organisations.

A rapid multi-residue method was developed for the determination of 16 herbicides in onion. The analytical procedure was based on preventing formation of sulfur-containing compounds in onion by microwave inactivation of the enzyme alliinase. The onion samples which had been pretreated were extracted with acetonitrile and cleaned by solid-phase extraction. The herbicide residues in onion were detected by gas chromatography/mass spectrometry with selected ion monitoring. The recoveries of 16 herbicides ranged from 69.2% to 105.0% with the relative standard deviations (RSD) below 10.7%. The limit of quantitation (LOQ) ranged from 0.003 to 0.015 mg kg−1. The method was applied to the analysis of herbicide residues in onion samples.

The hazards of endocrine disrupting pesticides on human and animal and the process on endocrine disrupting pesticides including the types,the mechanisms of effects and test,and evaluation technology was reviewed.Advances in the screen and test technology of EDCs was introduced.

A rapid method based on Enzyme-linked immunosorbent assay (ELISA) was developed for determination of endosulfan isomers and endosulfan sulfate in commercial black and green tea. The average recoveries obtained from crude extracts ranged from 86.6% to 91.1%, which from purified extracts ranged from 68.7% to 123%. The liner range of the standard curve was from 1 μg/L to 10 μg/L. The detection limit of crude extract was 2 mg/kg and the purified was 0.06 mg/kg. This study showed a validation of endosulfan analyzed in tea by ELISA and GC-MS at different levels and proved that ELISA is a quick, low cost and practical technique for monitoring pesticides in tea.

A method for the determination of sorbic acid and benzoic acid in fast frozen food by gas chromatography was studied. The sample was mashed at a high speed, sorbic acid and benzoic acid was extracted by petroleum ether and diethyl ether. The fat in the sample would be removed by changing pH of the liquor. Sorbic acid and benzoic acid were detected by GC with FFAP capillary column and FID. The recoveries were 90.5%-96.9% and the relative standard deviation was 1.4%-6.3%. The detection limit was 1 mg/kg.

土壤中吡虫啉残留量采用甲醇浸泡超声波提取,SPE小柱净化,HPLC的PDA检测器检测,回收率>83%,最低检出浓度为0.005mg/kg,可满足土壤中吡虫啉残留量的检测要求.

籽粒苋(Grain Amaranth)属苋科(Amaranthaceae),是一年生草本植物,其种子、叶和茎营养丰富,蛋白质和氨基酸含量较高,且维生素、铁、磷和钙含量亦高出一般作物.我国有丰富的饲用苋资源,年种植面积100多万亩.籽粒苋具有抗旱、耐盐碱、耐酸、再生力强、高产易栽培等特点,籽实每亩可产180～280公斤,兼收青茎叶4000～6000公斤,籽实是良好的食品资源和优质饲料,茎叶是优质青贮饲料,因此籽粒苋是具有良好开发前景的优质饲料.

通过对7种蔬莱不同处理的硝酸盐及亚硝酸盐含量进行分析,探讨其在蔬莱中的变化情况,为人们合理食用蔬菜提供科学依据.

籽粒苋(Grain Amaranth)是一种新型的一年生粮食作物,其营养价值极为丰富,高于一般的粮食作物.籽粒苋中含有丰富的微量元素,铁、钾、钙、镁含量尤其高,是人类补钙补铁的优质食品.本实验用原子吸收法对籽粒苋籽实和叶中的多种微量元素进行的含量分析,旨在利用其丰富的微量元素,开发新型的优质高效补钙、补铁食品.