The increasing interest in seaweed as a potential feed and food source has prompted concerns regarding the presence of potentially toxic arsenic (As) species. Seaweed is known to contain the organic As species arsenosugars, but the toxicity of these compounds is not fully known due to a lack of scientific data. Nori (Porphyra spp.), a common red seaweed and potential feed and food candidate, contains arsenosugar-phosphate (As-Sug-PO4) as a primary As species. In this study, As-Sug-PO4 was isolated from nori by solid-phase extraction (SPE) and subsequently evaluated for in vitro toxicity in primary salmon hepatocytes. The As-Sug-PO4 isolate, alongside As reference standards (arsenite As(III) and dimethylarsinic acid (DMA(V)), and arsenic-containing hydrocarbon (AsHC-360)), were assessed for cytotoxicity (mitochondrial activity) and for transcriptional effects on genes associated with lipid biosynthesis, inflammation and oxidative stress by reverse transcription-quantitative polymerase chain reaction (RT‑qPCR). Impacts on total As and As speciation were quantified in both cell pellets and culture medium using high-performance liquid chromatography (HPLC) coupled to inductively coupled plasma mass spectrometry (ICP-MS). High concentrations of As-Sug-PO4 caused cytotoxicity and mitochondrial damage, while lower levels influenced triglyceride biosynthesis, indicating impaired lipid deposition. As(III) was the most toxic As species, followed by As-Sug-PO4 and DMA(V) and unlike As(III), As-Sug-PO4 and DMA(V) did not alter total As levels and speciation profile in samples after 48 h of exposure. Follow-up in vivo studies with commercial standards are needed to confirm effects on lipid deposition and to support the establishment of regulatory limits for As-Sug-PO4 in feed and food.

The growing demand for sustainable aquaculture feeds has increased interest in novel marine ingredients. Blue mussels represent a locally available resource in Norway with potential for inclusion in Atlantic salmon diets. This study aimed to assess the effect of processing on nutrient bioavailability by combining in vitro hydrolysis and in vivo digestibility assays of blue mussel products using different processing methods and thermal treatments. Sixteen blue mussel products were produced using slurry and silage processing combined with different heat treatments. An initial in vitro screening was conducted, followed by validation in an Atlantic salmon digestibility trial. Nutritional composition, buffering capacity, and protein hydrolysis were used to select four representative products. Processing method and intensity significantly influenced nutrient composition and in vitro amino acid release (P < 0.05). Silage products exhibited higher ash content and lower true protein levels, primarily due to residual shell material and non-protein nitrogen fractions. Mild thermal treatment of slurry maintained amino acid release, whereas more intensive heating reduced protein hydrolysis. The in vivo trial showed a similar pattern, with gently processed slurry exhibiting higher protein and amino acid digestibility than more intensively treated products, while the silage solid fraction showed lower protein digestibility but relatively high lipid digestibility. Overall, the combined in vitro–in vivo approach demonstrated that moderate thermal processing preserves protein quality and nutrient bioavailability, whereas excessive heating impairs nutrient utilization. These findings demonstrate the value of sequential in vitro screening and in vivo validation for optimizing blue mussel processing for Atlantic salmon feeds.

This study examined how lighting conditions (White, or a combination of Red + Blue LEDs; and supplemental UV-C: 0, 1 or 3 applications) and cultivars (Daikon, Red Rambo) affected the colour, total phenolics content, antioxidant activity and individual polyphenols of radish microgreens (Raphanus sativus L.) in a sustainable vertical farming system, as well as the implications for polyphenolic intake, connecting agronomic practices with consumption. Light treatments preserved the visual appearance of all cultivars (Delta E < 4.4), maintaining market acceptance. Individual compounds varied slightly between cultivars, along with their responses the different lighting conditions. Considering 100 g fresh microgreens, Red Rambo provided higher levels of polyphenols (155-188 mg 100 g(- 1)) than Daikon (113-127 mg 100 g(- 1)), as well as higher antioxidant activity and total phenolics content, irrespective of growing light conditions. The choice of LED light during cultivation also significantly impacted polyphenolic levels: generally, White LEDs, optimized with 1 application of UV-C, favoured phenolic production in Daikon, while Red + Blue LEDs were more effective for Red Rambo regardless of UV-C. Sinapoyl malate was the most abundant compound in both cultivars. Red Rambo also provide complex anthocyanins, whose levels (69-80 mg 100 g- 1 total anthocyanins) are enhanced by Red + Blue LEDs, making this cultivar grown under this LED combination a particularly appealing option with greater potential health benefits. These findings highlight the value of microgreens amid growing consumer interest, as sustainable, visually appealing, and bioactive compound-rich foods that support daily vegetable intake.

Radishes are rich in health-promoting organosulfur compounds. This study investigated the effects of gastrointestinal digestion (GID) on glucosinolates (GSLs) and isothiocyanates (ITCs) of two radish microgreens cultivars (Raphanus sativus; cv. Daikon, and cv. Red Rambo) grown in a closed vertical system under either White (W) or Red+Blue (R + B) LEDs, combined with varying applications of UV-C radiation (0, 1, or 3 pulses). The individual and combined effects of LEDs and UV-C on GSLs and ITCs were evaluated before and after GID (INFOGEST static in vitro GID model). Relationships between compounds before/after digestion were explored through Structural Equation Modelling (SEM). Both cultivars are good sources of aliphatic GSLs (Daikon: 513.4 ± 23.1-681.5 ± 31.9, Red Rambo: 447.5 ± 38.5-532.0 ± 20.2 mg 100 g-1) and ITCs (Daikon: 0.9 ± 0.1-1.8 ± 0.3, Red Rambo: 1.3 ± 0.2-5.2 ± 1.0 mg 100 g-1). LED type was more impactful than UV-C radiation. Generally, R + B LEDs enhanced GSLs due to increasing dehydroerucin in Daikon, while improving ITCs in Red Rambo. Post-digestion, Red Rambo showed markedly higher sulforaphene levels compared to Daikon. Red Rambo's SEM significantly confirmed the conversion of glucoraphenin into sulforaphene and sulforaphane during GID. In Daikon, no significant relationships between compounds before/after GID were found. These findings suggest cultivar-specific metabolic pathways and responses to light, which can be optimized to enhance the accumulation of health-related compounds. The innovative use of SEM provided deeper insights into the metabolic conversions occurring during GID. The abundant sulforaphene levels from Red Rambo highlight this cultivar as an excellent source of this metabolite and its potential health benefits.

The present work aimed to evaluate protein and amino acid (AA) digestibility/solubility of different black soldier fly larvae (BSFL) based meals for Atlantic salmon in vivo and in vitro . Three types of insect meals that had been through different processing techniques were included: microwave full fat BSFL meal (BSFM), defatted BSFL meal with an enzymatic pre-treatment (BSFE) and a defatted BSFL meal without enzymatic pre-treatment (BSFH). For the in vivo digestibility studies only two ingredients (BSFE and BSFH) were used. The experimental diets for the different ingredients were prepared by mixing a control diet with BSFL meals at an 80:20 ratio. The in vitro method implied a two-stage hydrolysis involving both gastric simulation (acid hydrolysis) and gastrointestinal simulation (acid hydrolysis followed by alkaline hydrolysis), using enzymes extracted from salmon. The results showed that the AA solubility was higher in the gastrointestinal phases than the gastric phase alone, showing the importance of having both phases in vitro solubility for an effective protein breakdown. The AA solubility of different insect-based meals showed that neither partial defatting nor the addition of enzymatic treatment impacted the protein and AA solubility. The in vivo trial (56 days) recorded no differences between fish fed diets containing BSFE and BSFH for growth or body indices. The protein and AA apparent digestibility were similar for both BSFE and BSFH ingredients. Thus, in the current study no differences in nutrient digestibility were observed due to different processing methods employed to BSFL meal both in vivo and in vitro .

The intestinal barrier is crucial for gut health, and its dysfunction can lead to chronic inflammatory conditions. Food contaminants like pesticides, heavy metals, mycotoxins, heterocyclic aromatic amines (HAAs), and polycyclic aromatic hydrocarbons (PAHs) may worsen these conditions. However, research often overlooks the complex interactions between contaminants and the different cells in the intestine. This study explores the effects of repeated exposure to a mixture of 45 food contaminants on a triculture cell model (Caco-2/HT29-MTX monolayers and THP-1 cells) under normal and inflamed conditions. Two exposure scenarios were tested: a low-level mixture (LOW) reflecting daily diet exposure and a worst-case (WS) scenario. Cytotoxicity, barrier integrity, the expression of inflammation, oxidative stress, and intestinal barrier-related genes, as well as intestinal compounds absorption, were assessed. LOW and WS tested mixtures were confirmed to be non-cytotoxic to the monolayer. The inflamed model was validated with a 23 % reduction in TEER value compared to the normal. The WS mixture caused a maximum TEER decrease of 61 % after repeated exposure. The inflamed model was more vulnerable to contaminants, though the normal also showed significant effects. Gene expression revealed modulation of inflammation and oxidative stress, indicating that even real-life levels of food contaminants exacerbate these responses. The WS mixture induced 6-8 times the expression of inflammatory cytokines IL-8 and IL-1 beta. No significant differences (p > 0.05) were found in contaminants absorption between the models, but the absorption patterns align with known data. These findings enhance understanding of the intestinal effects of exposure to food contaminant mixtures in the diet.

This study characterizes the metabolomic profiles of three reference apricot cultivars (‘Bergeron’, ‘Currot’, and ‘Goldrich’) using 1H NMR spectroscopy and untargeted UPLC-QToF MS/MS to support plant breeding by correlating metabolomic data with fruit phenotyping. The primary objective was to identify and quantify the key metabolites influencing fruit quality from a nutraceutical perspective. The analysis revealed significant differences in primary and secondary metabolites among the cultivars. ‘Bergeron’ and ‘Goldrich’ exhibited higher concentrations of organic acids (109 mg/g malate in ‘Bergeron’ and 202 mg/g citrate in ‘Goldrich’), flavonoids such as epicatechin (0.44 mg/g and 0.79 mg/g, respectively), and sucrose (464 mg/g and 546 mg/g), contributing to their acidity-to-sugar balance. Conversely, ‘Currot’ showed higher levels of amino acids (24.44 mg/g asparagine) and sugars, particularly fructose and glucose (79 mg/g and 180 mg/g), enhancing its characteristic sweetness. These findings suggest that metabolomic profiling can provide valuable insights into the biochemical pathways underlying apricot quality traits, aiding in the selection of cultivars with desirable characteristics. The integration of phenotyping data with 1H NMR and UPLC-QToF MS/MS offers a comprehensive approach to understanding apricot metabolomic diversity, crucial for breeding high-quality, nutritionally enriched fruits that meet market demands.

Radish microgreens contain (poly)phenols, whose fate after gastrointestinal digestion remains largely unknown. This study investigated the in vitro digestion and colonic fermentation of two radish microgreen (Raphanus sativus L.) cultivars, Daikon and Red Rambo, grown under different lights in vertical farming, exploring how cultivar and light influence (poly)phenol catabolism. After digestion, most (poly)phenols remained in the residual fraction, which was subjected to microbial fermentation, resulting in a large set of catabolites. Cultivar differences outweighed the effects of light treatments, as Red Rambo retained twice the (poly)phenols of Daikon, influencing fermentation outcomes. 4'-Hydroxy-3',5'-dimethoxycinnamoyl malate and sinapoyl choline dominated both cultivars, while flavonoids and anthocyanins in Red Rambo contributed with further complexity. Microbiota-derived catabolites included low molecular weight compounds such as 3-(3',4'-dihydroxyphenyl)propanoic and 3-(4'-hydroxy-3'-methoxyphenyl)propanoic acids, along with 4'-hydroxy-3',5'-dimethoxycinnamic, 4'-hydroxy-3'-methoxycinnamic, 4'-hydroxycinnamic, and 3-(4'-hydroxyphenyl)propanoic acids. Hypothesised catabolic pathways were outlined, with 4'-hydroxy-3',5'-dimethoxycinnamoyl malate and sinapoyl choline microbial catabolism being outlined for the first time.

Brassica microgreens are rich in phytochemicals and are attractive crops for controlled vertical farming systems where the light spectrum can be precisely manipulated. Understanding the effects of pre-harvest hormetic UV-C light doses on plant composition and growth parameters represents a novel and largely unexplored area for precision agriculture and nutrition. Therefore, the objective of this work was to investigate the impact of exposing red mustard microgreens to low/hormetic doses of UV-C radiation on their growth, chemical composition and colour. Plants were grown in a controlled environment and exposed to 0.3 kJ m-2, 254 nm UV-C radiation at the end of the cultivation period. Treatments included a single pulse on day 7, or three pulses at days 7, 8, and 9 and harvest on day 10. UV-C radiation presented a hormetic effect, while 1 pulse of UV-C stimulated growth and productivity without significant colour changes in microgreens, 3 pulses of UV-C radiation to did not show significant effects when compared to controls (no UV-C exposure). Moreover, strong negative correlations were observed between growth parameters and chemical composition (p<0.05). Microgreens with enhanced growth parameters showed a decrease in phenolic compounds content and antioxidant activity. Interestingly, regardless of quantification, untargeted metabolomics using UHPLC-Q-Orbitrap-MS/MS revealed that the secondary metabolites profile remained similar between control and microgreens treated with UV-C radiation.

Consumers are regularly exposed to well-known food contaminants (FCs), which are typically assessed for risk on an individual basis. However, there is limited knowledge about the overall levels and combinations of these compounds depending on dietary choices. The goal of this study was to estimate the real-life mixtures of FCs in different dietary models by integrating extensive data from the scientific literature concerning the reliable quantification of FCs in foods. A FAIR database detailing the occurrence of 73 FCs in 16 foods commonly consumed was built. The data were integrated into an omnivorous and a vegetarian dietary model. A weighted estimate of the 25th, 50th, and 75th percentiles of FCs in both dietary models revealed that the omnivorous model presented slightly higher levels of FCs than the vegetarian. At the 25th percentile, the FC levels in both dietary models fall within the European Food Safety Authority (EFSA) reference exposure levels for chemical hazards, except for arsenic, lead, cadmium, fumonisin B1, and OTA. At the 75th percentile, the FC levels exceed the EFSA reference levels for those FCs and additional mycotoxins. Using in vitro models, the 25th percentile can mimic real-life FC exposure, while the 75th percentile simulates a possible worst-case scenario.

Identifying metabolism and detoxification mechanisms of Hg in biota has important implications for biomonitoring, ecotoxicology, and food safety. Compared to marine mammals and waterbirds, detoxification of MeHg in fish is understudied. Here, we investigated Hg detoxification in Atlantic bluefin tuna Thunnus thynnus using organ-specific Hg and Se speciation data, stable Hg isotope signatures, and Hg and Se particle measurements in multiple tissues. Our results provide evidence for in vivo demethylation and biomineralization of HgSe particles, particularly in spleen and kidney. We observed a maximum range of 1.83 parts per thousand for delta 202Hg between spleen and lean muscle, whereas Delta 199Hg values were similar across all tissues. Mean percent methylmercury ranged from 8% in spleen to 90% in lean muscle. The particulate masses of Hg and Se were higher in spleen and kidney (Hg: 61% and 59%, Se: 12% and 6%, respectively) compared to muscle (Hg: 2%, Se: 0.05%). Our data supports the hypothesis of an organ-specific, two-step detoxification of methylmercury in wild marine fish, consisting of demethylation and biomineralization, like reported for waterbirds. While mass dependent fractionation signatures were highly organ specific, stable mass independent fractionation signatures across all tissues make them potential candidates for source apportionment studies of Hg using ABFT.

The content of salt, sugar, fat and fibre in processed foods plays an important role in health promotion and diet-related disease prevention. However, the reformulation of processed foods with respect to these nutrients, in order to obtain healthier products, is a major challenge for the industry. This due to their impact on other essential food characteristics, such as taste, appearance and texture, or even safety, convenience and price. Achieving comparable acceptance between original and reformulated products is thus a demanding endeavour, requiring different strategies depending on the nutrients and type of food being reformulated. The characteristics of different consumer groups also play an important role in the acceptance and marketplace success of nutritionally-enhanced products. This chapter describes key aspects of the product reformulation processes undertaken by the food industry to meet current global nutrition and public health goals. Different food science and technology approaches are detailed, along with some marketing strategies aimed at ensuring consumer acceptance and marketplace success of reformulated products.

Background: Blue mussels (Mytilus edulis L.) can accumulate undesirable substances, including the potentially toxic elements (PTEs) cadmium (Cd), mercury, (Hg), lead (Pb), arsenic (As) and As species. In this study, the levels of PTEs and As species were determined in samples of blue mussels to assess the influence of environmental and biological factors, and evaluate the potential risk associated with blue mussels in terms of food and feed safety. Methodology: Blue mussels were collected monthly from one location in Western Norway from February 2018 to December 2018, and from April 2019 to April 2020. Samples were analyzed for PTEs using inductively coupled plasma mass spectrometry (ICP-MS), and high-performance liquid chromatography (HPLC) coupled to ICP-MS. Temperature, salinity and fluorescence (chlorophyll a) were monitored in the seawater column by STD/CTD, to assess the potential influence of these environmental factors on the PTE levels in the mussels.Results: The results showed seasonal variations in the PTEs, with somewhat higher concentrations in spring and winter months. Unusually high levels of total As (101.2 mg kg-1 dw) and inorganic As (53.6 mg kg-1 dw) were observed for some of the time points. The organic As species arsenobetaine was generally the major As species (17-82% of total As) in the mussels, but also simple methylated As species and arsenosugars were detected. Principal components analysis (PCA) did not show a consistent relationship between the environmental factors and the PTE concentrations, showing contrary results for some elements for the periods studied. The condition index (CI) could explain variations in element concentration with significant correlations for Cd (r =-0.67, p = 0.009) and Pb (r =-0.62, p = 0.02 in 2019/20 and r =-0.52, p = 0.02 in 2018), whereas the correlation between As and CI was not significant (r = 0.12 in 2018, and r =-0.06 in 2019/20). Higher concentrations of iAs and arsenosugars coincided with increased signals of chlorophyll a, suggesting that phytoplankton blooms could be a source of As in the blue mussels. Conclusion: To our knowledge, this is the first study of As species in blue mussels collected over a time period of two years, providing an insight into the natural variations of these chemical forms in mussels. In terms of mussel as food and future feed material, concentrations of Cd, Hg and Pb were below the maximum levels (MLs) established in the EU food and feed legislation. However, levels of As and iAs in mussels at some time points exceeded the MLs for As in the feed legislation, and the margin of exposure (MOE) was low if these mussels were for human consumption, highlighting the importance of determining the chemical forms of As in feed and food.

This in vitro digestion method was used to evaluate amino acid solubility of different black soldier fly larvae (BSFL) meals and experimental diets for Atlantic salmon. Three types of insect meal that had been through different processing techniques included: a microwave full fat BSFL (BSFM), defatted BSFL meal with an enzymatic pre-treatment (BSFE) and a defatted BSFL meal without enzymatic pre-treatment (BSFH).

Microalgae and blue mussels are known to accumulate undesirable substances from the environment, including arsenic (As). Microalgae can biotransform inorganic As (iAs) to organoarsenic species, which can be transferred to blue mussels. Knowledge on As uptake, biotransformation, and trophic transfer is important with regards to feed and food safety since As species have varying toxicities. In the current work, experiments were conducted in two parts: (1) exposure of the microalgae Diacronema lutheri to 5 and 10 μg/L As(V) in seawater for 4 days, and (2) dietary As exposure where blue mussels (Mytilus edulis L.) were fed with D. lutheri exposed to 5 and 10 μg/L As(V), or by aquatic exposure to 5 μg/L As(V) in seawater, for a total of 25 days. The results showed that D. lutheri can take up As from seawater and transform it to methylated As species and arsenosugars (AsSug). However, exposure to 10 μg/L As(V) resulted in accumulation of iAs in D. lutheri and lower production of methylated As species, which may suggest that detoxification mechanisms were overwhelmed. Blue mussels exposed to As via the diet and seawater showed no accumulation of As. Use of linear mixed models revealed that the blue mussels were gradually losing As instead, which may be due to As concentration differences in the mussels' natural environment and the experimental setup. Both D. lutheri and blue mussels contained notable proportions of simple methylated As species and AsSug. Arsenobetaine (AB) was not detected in D. lutheri but present in minor fraction in mussels. The findings suggest that low-trophic marine organisms mainly contain methylated As species and AsSug. The use of low-trophic marine organisms as feed ingredients requires further studies since AsSug are regarded as potentially toxic, which may introduce new risks to feed and food safety.

Food remains a major source of human exposure to chemical contaminants that are unintentionally present in commodities globally, despite strict regulation. Scientific literature is a valuable source of quantification data on those contaminants in various foods, but manually summarizing the information is not practicable. In this review, literature mining and machine learning techniques were applied in 72 foods to obtain relevant information on 96 contaminants, including heavy metals, polychlorinated biphenyls, dioxins, furans, polycyclic aromatic hydrocarbons (PAHs), pesticides, mycotoxins, and heterocyclic aromatic amines (HAAs). The 11,723 data points collected from 254 papers from the last two decades were then used to identify the patterns of contaminants distribution. Considering contaminant categories, metals were the most studied globally, followed by PAHs, mycotoxins, pesticides, and HAAs. As for geographical region, the distribution was uneven, with Europe and Asia having the highest number of studies, followed by North and South America, Africa and Oceania. Regarding food groups, all contained metals, while PAHs were found in seven out of 12 groups. Mycotoxins were found in six groups, and pesticides in almost all except meat, eggs, and vegetable oils. HAAs appeared in only three food groups, with fish and seafood reporting the highest levels. The median concentrations of contaminants varied across food groups, with citrinin having the highest median value. The information gathered is highly relevant to explore, establish connections, and identify patterns between diverse datasets, aiming at a comprehensive view of food contamination.

The impact of dietary trace minerals (TM; zinc and selenium) and water temperature during early seawater phase on the development of vertebral deformities and cataracts in adult Atlantic salmon was studied. Two experimental feeds (control and High TM) and two water temperatures (12 and 16 degrees C) were designed in a 2 x 2 factorial arrangement. The Zn and Se levels in the control and high TM diet corresponded to 150 or 200 mg Zn/ kg and 0.5 or 0.7 mg Se/kg diet, respectively. Atlantic salmon post-smolts (mean weight, 138 g) were distributed in 12 tanks and were randomly assigned to one of the treatments in triplicate groups. The experimental regime lasted for 12 weeks and thereafter the fish were sampled or transferred to three net pens comprising all four treatments in a common garden. The fish were further grown on a common commercial feed and assessed for vertebral deformities and cataracts as adults (4 to 4.5 kg). Atlantic salmon post-smolt fed the control diet or reared at 16 degrees C grew more than those fed the high TM diet or reared at 12 degrees C at the end of the 12-week experimental feeding period. Vertebral mineral density, concentration of Zn and Se in the whole body, plasma and bile were influenced by the dietary treatments. The fast growth rates observed in the control diet and 16 degrees C exposed fish were associated with increased risk of vertebral deformities and cataract at adult stage. The preventive effect of the high TM diet on incidence and severity of vertebral deformities and cataracts was highly effective if the fish were reared at 12 degrees C during early seawater phase, but was reduced or absent if reared at 16 degrees C. Cataract inducing effect of high temperature at 16 degrees C was much stronger than the cataract mitigating effect of high TM diet at 16 degrees C. Overall, feeding Atlantic salmon post-smolts a high TM diet (Zn: 200 mg/kg; Se: 0.7 mg/kg diet) during early seawater phase for 12 weeks reduced the incidence of vertebral deformity and cataracts in adult fish, albeit subject to water temperature.

Background: Aquaculture aims to reduce the environmental and climate footprints of feed production. Consequently, low trophic marine (LTM) resources such as blue mussels and kelp are potential candidates to be used as ingredients in salmon feed. It is relevant to study potential undesirables associated with their use, as well as assessing food safety by investigating their transfer from feed-to-fish. The marine biota is well known to contain relatively high levels of arsenic (As), which may be present in different organic forms depending on marine biota type and trophic position. Thus, it is important to not only obtain data on the concentrations of As, but also on the As species present in the raw materials, feed and farmed salmon when being fed novel LTM feed resources. Methods: Atlantic salmon were fed experimental diets for 70 days. A total of nine diets were prepared: four diets containing up to 4 % fermented kelp, three diets containing up to 11 % blue mussel silage, and one diet containing 12 % blue mussel meal, in addition to a standard reference diet containing 25 % fish meal. Concentrations of As and As species in feeds, faeces, liver and fillet of Atlantic salmon were determined by inductively coupled plasma mass spectrometry (ICP-MS) and high-performance liquid chromatography coupled to ICP-MS (HPLC-ICP-MS), respectively. Results: The use of kelp or blue mussel-based feed ingredients increased the concentration of total As, but maximum level as defined in Directive 2002/32 EC and amendments was not exceeded. The concentrations found in the experimental feeds ranged from 3.4 mg kg-1 to 4.6 mg kg-1 ww. Arsenic speciation in the feed varied based on the ingredient, with arsenobetaine dominating in all feed samples (36-60 % of the total As), while arsenosugars (5.2-8.9 % of the total As) were abundant in kelp-included feed. The intestinal uptake of total As ranged from 67 % to 83 %, but retention in fillet only ranged from 2 % to 22 % and in liver from 0.3 % to 0.6 %, depending on the marine source used. Fish fed feeds containing blue mussel showed higher intestinal uptake of total As when compared with fish fed feeds containing fermented kelp. Fish fed fermented kelp-based feeds had higher retained concentrations of total As when comparing with fish fed feeds containing blue mussel. Despite relatively high intestinal uptake of total As, inorganic and organic As, the retained concentrations of As did not reflect the same trend. Conclusion: Although the use of LTM feed ingredients increased the level of total As in this feeds, salmon reared on these diets did not show increased total As levels. The well-known toxic inorganic As forms were not detected in salmon muscle reared on LTM diets, and the non-toxic organic AsB was the dominant As species that was

DIETxPOSOME database concerning literature selection of potentially useful papers retrieved from PubMed search API, concerning contaminants quantification in food items of worldwide highest supply and using FoodMine code (text matching filter) and machine learning (ML) protocols. A list of 2,442 papers that potentially contained relevant information, covering the period between 2000 and 2022, was compiled from an initial number of 1,932,345 papers.

Atlantic salmon fed low fish meal feeds supplemented with zinc (Zn) were studied in two feeding trials. In trial I, Atlantic salmon parr were fed six graded Zn levels (40 to 249 mg kg(-1) as ZnSO4) for 8 weeks in freshwater followed by a 4-week seawater phase. In trial II, Atlantic salmon post-smolt were fed for 10 weeks in SW with 10 dietary Zn levels (45 to 280 mg kg(-1)), either as ZnSO4 or Zn-glycinate. Growth was unaffected by dietary Zn in both trials. Dietary Zn affected concentration of Na+ and K+ ions in plasma, branchial and intestinal expression of sodium potassium ATPase, tissue and body Zn status, and cataracts. Seawater transfer significantly reduced apparent availability, body and tissue levels of Zn due to increased endogenous Zn loss. Atlantic salmon postsmolt in seawater improved body and tissue Zn status with increasing dietary Zn levels, irrespective of the Zn source. Body or tissue saturation of Zn occurred at dietary Zn levels between 137 and 156 mg kg(-1) with smolts in freshwater and 181 to 218 mg kg(-1) in SW post-smolts. Dietary Zn levels below 180 mg kg(-1) in low fish meal feeds compromised the Zn status and welfare of Atlantic salmon in seawater.