Introduction: This work aimed to create a new platinum-interferon conjugate and investigate its properties. Background: Multiple drug resistance of tumor cells is a serious challenge in modern medicine. Neoplastic diseases are often complicated by persistent bacterial infections, which impairs efficacy and the outcome of anticancer therapy. The development of new bifunctional drugs for the treatment of tumor growth and bacterial complications is an urgent task. Objective: The objective of this study was to evaluate the complex antiblastic and antibacterial properties of interferon-platinum conjugate in vitro. Material and Methods: The coordination complex of dichloro-N,N,N,N-tetrakis-(2-aminoethyl)- 1,6-hexamethylenediaminobisplatin dichloride was used for production of conjugate with human α-interferon. The binding of a metal complex with protein was established by means of radiochemical neutron activation analysis. As test objects, we used lympholeukemic Jurkat cells line, human lymphocytes in reaction of lymphocyte blastic transformation and different strains of microorganisms, including Staphylococcus aureus and Pseudomonas aeruginosa. Results: Binuclear platinum human α-interferon conjugate has a significant dose-dependent antibacterial and antiproliferative suppressive effect on target cells and bacteria. Conclusion: The tested preparation can be of a certain interest in drug designing for rational antiblastic therapy, especially in the case of infectious complications. This conjugate can be a convenient platform for creating drugs for the complex therapy of oncological diseases with bacterial complications.

Background: Developing new antibacterial and antiviral drugs are considered a significant issue due to the emergence and spread of resistant strains of microorganisms. The COVID-19 pandemic has dramatically increased the need for new broad-spectrum anti-infective agents. Objective: This experimental study aimed to investigate the antibacterial and phagocytic properties of silver-interferon preparation. The combination of properties of complex drugs makes them promising for treating drug-resistant infections and bacterial complications of viral diseases. Method: The antibacterial effect of the silver-interferon platform was investigated by agar diffusion and serial dilution methods. The drug's effect on the functional activity of phagocytes was studied on human neutrophils in a Staphylococcus aureus uptake test. Results: Investigations have shown that the silver-interferon complex possesses a bactericidal mechanism of action against tested bacterial strains, including Streptococcus pneumonia, Salmonella enteritidis, Staphylococcus aureus, Escherichia coli. Streptococcus pneumonia was the most susceptible bacterial target for the tested complex, with a growth inhibition zone of 12±0.6 mm and a minimal bactericidal concentration of 0.08 mg/ml. A slight stimulating action of the drug in relation to the activity of phagocytes was revealed. Conclusion: Silver-interferon has proved as a prospective anti-infective drug with a wide range of activities.

Background: The fight against infectious diseases includes two main components – immediate direct anti-infective action and stimulation of one's own immunity. Objective: In this study, we investigated the properties of diamond nanoparticles modified with gold. The use of such gold nanoparticles as indirect anti-infectious agents and immunostimulators has certain prospects. Materials and Methods: Gold hydrosols were synthesized by the reduction in an aqueous solution of gold (III) with sodium citrate (Na3Cit) under heating. Modification procedure of nanodiamond by gold requires incubation, a small sample of nanodiamond in a defined volume of a gold sol for about 24 hours in a dark place. We use human blood cells as test objects. The reaction of blastic transformation of lymphocytes was applied here as a test of biological actions of modified nanodiamond. Results: Modified nanodiamond do not have a toxic influence on blood cells. Modified nanodiamond possesses stimulation effects on spontaneous proliferation of lymphocytes and does not significantly affect phytohemagglutinin-induced proliferation. Nanodiamond slightly increases phagocytosis parameters of neutrophil leucocytes. Conclusion: Thus, results showed that the nanodiamond modified by gold possesses immunostimulating activity, increases the phagocytic activity of neutrophilic leukocytes and stimulates lymphocytes in the spontaneous proliferation test. Gold-modified nanodiamond could be considered as a non-direct anti-infective agent through immune stimulation.

Background: Multidrug Resistance (MDR) of microorganisms is the biggest pharmacological challenge, despite plenty of modern antibiotics. Obviously, new classes of antimicrobial drugs against MDR pathogens need to be developed. Objective: This work aimed to investigate the antibacterial properties of the novel copper-based complex - bisimidazoles - (1,10) phenanthroline cuprum (II) dichloride - [CuPhenIm2]Cl2. Material & Methods: The complex - bisimidazoles-(1,10)phenanthroline cuprum (II) dichloride - [Cu- Phen-Im2]Cl2 was synthetized for the experiment. The structure was identified by elemental analysis and IR spectroscopy. The antibacterial properties of the copper complex were investigated by agar diffusion and serial dilution methods with the following bacteria strains Staphylococcus haemolyticus (ATCC 29970), Staphylococcus aureus (ATCC 25923), Proteus vulgaris (ATCC 13315), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Mycobacterium bovis (Valle). Antituberculosis activity test included evaluation of bacterial growth on Lowenstein Jensen media with a serial dilution of copper complex, within an observation period of 90 days. Results: The prospect copper complex showed significant antibacterial activity. The most susceptible to copper action were strains of S.haemolyticus and S.aureus. The bisimidazoles - (1,10) phenanthroline cuprum (II) dichloride revealed moderate bactericidal action against Mycobacteria bovis. Conclusion: Most of the tested bacterial pathogens were highly susceptible to the novel copper substance. A detailed assessment of the biological action in in vivo is further required.

Objective: Important trend in the global agribusiness is restrictions of antibiotics content in meat and poultry products. Widespread of antibiotic-resistant microorganisms leads to huge losses of the effectiveness of the drugs. The aim of the present work was an investigation of the biological activity of antibacterial complex–silver methenamine poultry farming.Methods: The study of biochemical and hematological indices of blood we performed one day after single and course of oral drug administration on broilers Ross 308. Evaluation of biological action in industrial poultry farming was carried out on a group of 30 thousand broilers after a course of oral administration.Results: revealed the silver complex (in form of water solution) has no toxic effects on blood parameters. It was shown the positive influence on survival and productivity of broilers.Conclusion: Methenamine silver has certain potential as prospect drug and food additive.

The paper presents the results of investigations of genotoxicity and morphological characteristics of nanoscale gold particles prepared by the electric spark dispersion method in a water medium. To investigate the properties we used comet assay, transmission electron microscopy, thermal desorption of nitrogen (BET). It was revealed, gold particles did not cause DNA damage at low concentrations (0.01 - 0.1 mg/ml) and detectable level of genotoxicity of gold nanoparticles is observed at concentrations of 0.3 mg/ml and above.

Background: Developing of novel pharmaceutic agents is an actual topic for chemotherapy. Heavy metals possess a significant cytotoxic properties and some of them are widly introduced in medical practice, e.g. cisplatin.Aim and Objective of this study was to investigate the cytostatic effects of prospective silver-based preparation on viral bovine leukemia model in vivo and lymphoblast cells in vitro.Material and Methods: Silver salt of methenamine was suggested as prospective substance and tested was tested on black-motley cattle with bovine leukemia to reveal influence on hematological parameters. Preparation was applied in form of 0.5% solution in different doses and was administered as single intravenous injection. Blood parameters were determined by triple hematological monitoring. Investigation of cytostatic activity was conducted on the leukemic cells of HUT-102 line in Vitro in concentration range of 0.03-3.0 mg/ml.Results: The results showed normalization of blood parameters in dose-dependent manner after intravenous injection of methenamine silver salt. It was revealed a pronounced inhibitory effect of silver-based drug on the leukemic cells both in vitro and in vivo. Control blood test one month after experimental therapy confirmed the stability of the achieved results.Conclusion: The tested drug showed a moderate cytostatic effect. Methenamine silver nitrate can be considered as prospective pharmacological agent. Bovine leukemia was shown as a convenient model for the development of new treatment of blood diseases. Continuation of the work is of interest for both veterinary and medical pharmacotherapy.Keywords: methenamine silver nitrate, HUT 102 cells; lymphocytes, leucosis, cytostatic activity, bovine leukemia, silver hexamethylenetetramine.

Purpose: The problem of infectious diseases and drug resistance is becoming increasingly important worldwide. Silver is extensively used as an anti-infective agent, but it has significant toxic side effects. In this regard, it is topical to develop new silver compounds with high biological activity and low toxicity. This work is aimed to study DNA damage and chromosomal aberrations in blood cells under the influence of new silver-based compound of general formula C6H19Ag2N4LiO6S2, with antiviral activity. Methods: The comet assay was applied for the genotoxic affects assessment on mice blood leukocytes. DNA damage was determined bases on the percentage of DNA in a comet tail (tail DNA), under the influence of silver complex in different concentrations. Genotoxic effect of the tested substance on the somatic cells was determined by chromosomal aberration test of bone marrow cells of mice. Results: In the course of the experiments, no essential changes in the level of DNA damage in the cells were found, even at highest concentrations. The administration of the substance in doses up to 2.5 g/kg in mice did not cause any increase in the frequency of chromosomal aberration in bone marrow cells. Conclusion: Taking into account known silver drug genotoxic properties, the use of a given complexed silver compound has possible great advantages for potential applications in the treatment of infectious diseases.

Introduction: Currently, developing new antibacterial drugs as alternative antibiotics is a very active area of research, due to widspreading widespread prevalence of resistant strains of microorganisms. This work intends to investigate of antibacterial properties and influence on immune blood cells of the silver-based compound hexamethylenetetramine (methenamine) silver nitrate with general formula [Ag(CH2)(6) N-4]NO3.Materials and Methods: The antibacterial effect of the silver complex was investigated by agar diffusion and serial dilution methods. Silver complex have been investigated for its impact on the phagocytic activity of neutrophils and on immune cells during the reaction of blast transformation of lymphocytes (RBTL).Results: Studies have shown that hexamethylenetetramine silver nitrate possesses both bactericidal and bacteriostatic dose-dependent effect on tested bacterial strains, including Staphylococcus aureus, Proteus vulgaris, Pseudomonas aeruginosa, Streptococcus pneumoniae. Escherichia coli were shown to be the most susceptible bacteria. Cytotoxic effect of silver salt on lymphocytes was detected in high dosage in RBTL. No significant immunosuppressive impact on neutrophils phagocytic activity of tested complex was shown.Conclusion: Agents of nosocomial infections were highly susceptible to the drug. Complex has proved to be promising as a prospective antibacterial drug with wide range of activity.