<正>学龄前儿童处于视功能发育敏感期内,异常屈光状态极易导致弱视。弱视是儿童时期可治疗的视力缺陷,散光性屈光不正是儿童弱视的重要危险因素,为了了解学龄前儿童散光屈光度的变化规律以及散光作为弱视危险因素所占的比例,我们对石家庄市区及石家庄市山区部分儿童进行屈光筛查及外眼、眼底检查,报告如下。1资料与方法1.1一般资料2011年9月至11月对石家庄市区和石家庄市山区各2所幼儿园3～6岁学龄前儿童进行筛查。按年龄分组3岁～组、4岁～组、5岁～组、6岁～组。

BACKGROUND:There have been no standard procedures regarding how to simply and efficiently separate and purify human peripheral blood monocytes and stimulate them into dendritic cells.OBJECTIVE:To investigate the efficacy of purification of human peripheral blood monocytes on gelatin-coated surfaces,analyze the phenotype of dendritic cells generated by these monocytes,and make a comparison with conventional plastic adhesion method.METHODS:Human peripheral blood mononuclear cells were harvested by Ficoll-Hypaque gradient centrifugation.Gelatin group and common plastic group were designated according to coating flasks with or without gelatin.Monocytes were harvested from each group and then were stimulated into dendritic cells.The number of monoctyes,CD14 positive rate of monocytes,contaminated T,B lymphocytes,the expression of CD1a,CD83 on immature and mature dendritic cells were determined.Cell viability was determined by trypan blue staining.Platelet contamination was compared between gelatin and common plastic groups.RESULTS AND CONCLUSION:The number of monocytes and CD14 positive rate were significantly higher in the gelatin group than in the common plastic group(P < 0.05).Contaminated T,B lymphocytes were significantly more in the common plastic group than in the gelatin group(P < 0.05).There were no significant differences in cell viability and dendritic cell phenotype between gelatin group and common plastic group(P > 0.05).Platelet contamination rate was significantly lower in the gelatin group than in the common plastic group.Gelatin-coated surfaces can effectively and simply isolate monocytes and successfully stimulate them into mature dendritic cells.

目的分析小儿纤维支气管镜术中及术后出现的并发症及原因,以探讨临床适宜的防治措施。方法分析2007年1月至2010年1月行纤支镜患儿400例(男208例,女192例),按年龄分为婴幼儿组(6月～3岁,94例),学龄前组(4～6岁,128例)及学龄组(7～14岁,178例)。使用Olympus3C40或P40纤维支气管镜,根据年龄、体重、病情等选用不同型号的纤支镜。记录3组术中、术后并发症发生情况并进行统计分析,探讨并发症发生的可能原因和对策。结果本研究所有患儿398例(99.5%)顺利进镜,其中术中出血者55例(13.8%),学龄前组高于婴幼儿组及学龄组,差异有统计学意义(P<0.05);紫绀22例(5.5%),婴幼儿组高于学龄前组及学龄组,差异有统计学意义(P<0.05);术后发热23例(5.8%),差异无统计学意义(P>0.05)。结论小儿纤支镜术临床应用过程中应合理选择镜型,并根据患儿年龄、病情等因素充分估计可能发生的并发症,及时发现,及时处理,确保纤支镜术安全进行。

<正>在人的生命历程中,临终是生命最终的发展阶段。死亡是人生旅途的终点,尽管人们千方百计地逃避或否认死亡,但对每个人而言,死亡都是不可避免的。随着社会的进步、医学的发展,临终关怀护理越来越受到重视[1]。临终护理是指针对死

目的评价胺碘酮联合稳心颗粒与胺碘酮治疗急性心肌梗死(AMI)并室性心律失常的疗效和安全性。方法 204例AMI患者完全随机分为胺碘酮联合稳心颗粒组和胺碘酮组,每组102例。所有患者均按AMI常规处理,胺碘酮组予胺碘酮首剂150mg+0.9%氯化钠溶液20ml,10min内注入,间隔10～15min可重复,随后以1.0～1.5mg/min滴注维持6h,之后依病情减少剂量至0.5mg/min,直至仅出现偶发室性早搏,停止静脉给药,改口服胺碘酮600mg/d连续7d,此后改400mg/d连续7d,最后改为200mg/d维持治疗1个月,再减至200mg,1次/d,每周5d,间歇2d,维持6个月。胺碘酮联合稳心颗粒组在胺碘酮组治疗的基础上加服稳心颗粒,1袋(9g)/次,3次/d,维持6个月。结果胺碘酮联合稳心颗粒组,显效40例(39.2%),有效48例(47.1%),总有效率86.3%;胺碘酮组患者显效30例(29.9%),有效44例(43.1%),总有效率72.5%。2组差异有统计学意义(P<0.05)。无恶化病例,未出现严重不良反应。随访6个月未出现甲状腺功能异常及严重心律失常的发生。结论胺碘酮和稳心颗粒联合治疗AMI后室性心律失常效果满意,安全可靠,值得临床推广使用。

2002年实施卫X项目后,石家庄市利用现有的医疗卫生服务网络,加大行政干预,积极发挥综合医院和社区医疗的作用,加强以结核病防治机构为中心的结核病归口管理工作,促进了肺结核病人的发现和管理,现报告如下.

1 临床资料患者男,35岁.阴囊红斑、痒反复发作半年.患者半年前发现阴囊出疹,红色斑片状,有痒感.查真菌阳性,采用特比萘芬乳膏治疗,症状好转.