目的 分析急性细菌感染发生压力性损伤患者的临床特点及微循环状态改变情况.方法 选取首都医科大学附属北京朝阳医院急诊科2022年1月至2022年6月收治的急性细菌感染患者70例,根据入院5d内是否发生皮肤压力性损伤分为压力性损伤组和非压力性损伤组.比较两组患者的基本临床资料,包括年龄、性别、入院营养状态评价、既往病史、急性感染病灶部位、机械通气及28d死亡情况;入院24、48、72h血常规(白细胞计数、血小板计数、血红蛋白)、D-二聚体、血乳酸、碱剩余、降钙素原、C反应蛋白等指标;序贯器官衰竭评分及急性生理与慢性健康Ⅱ评分;舌下微循环监测参数(包括De Backer评分、小血管密度、微血管血流指数、血流不均一指数、灌流血管比例和灌流血管密度).结果 共52例患者完成本次研究,其中21例(40.4％)发生压力性损伤,作为压力性损伤组,其余31例为非压力性损伤组.24例(46.2％)患者最终诊断为脓毒症,压力性损伤组17例(81.0％),非压力性损伤组7例(22.6％),两组差异有显著性(P＜0.05).随访28d,共10例患者死亡,其中压力性损伤组4例(19.0％),非压力性损伤组6例(19.4％),两组差异无显著性(P＞0.05).两组患者年龄、性别、入院营养状态评价、基础疾病、感染病灶部位、实验室指标等方面差异均无显著性(P＞0.05).压力性损伤组入院72h的序贯器官衰竭评分高于非压力性损伤组(P＜0.05),且压力性损伤组与非压力性损伤组入院48h和72h的序贯器官衰竭评分均高于同组入院24h(P＜0.05).压力性损伤组入院24h平均动脉压低于非压力性损伤组(P＜0.05),入院24、48、72h血乳酸水平均高于非压力性损伤组(P＜0.05),碱剩余水平低于非压力性损伤组(P＜0.05).压力性损伤组入院24、48、72h的微循环流量指数、灌注血管比例和灌注血管密度均低于非压力性损伤组,血流不均一指数高于非压力性损伤组,差异有显著性(P＜0.05).压力性损伤组舌下微循环各参数的48h变化率与72h变化率比较差异无显著性(P＞0.05).结论 急性细菌感染患者在发生压力性损伤时,微循环状态发生异常,舌下微循环监测在急性细菌感染患者压力性损伤的防治方面具有潜在应用价值.

人的生命之旅就像一条单行线,每个人都向着既定的终点踯躅前行.不过,总会有一些意外闯入,迫使我们加快前行的脚步,甚至直接导致人生道路戛然而止.猝死,就是最可怕的意外.

目的 通过研究医联体(社区)医师在急救技能掌握与培训方面存在的问题,以提高并改进住院医师规范化培训工作.方法 对北京朝阳医院医联体的77位医师进行为期两年的随访,通过问卷调查、针对性培训、技能考核以及自我评价等方式,分析社区医生在临床工作中发生技能掌握与培训脱节的深层次原因,从临床需求提出解决方法,并用于住培医师规范化培训质量的提升.结果 77位医师参加了此次研究,年龄(33.98±6.76)岁,男性34位(44.2％),女性43位(55.8％),自评危重症抢救能力(6.09±1.76)分,自评抢救时心理压力(6.52±1.84)分,自评团队配合(7.12±1.84)分,在诊治患者中面临的困难数量(2.57±1.45)个,工作年限与接诊患者的心理压力存在显著负相关关系(r=-0.400,P=0.000).住培组医生的年龄小于非住培组(岁:28.09±2.43 vs.38.90±5.03,P=0.03),工作年限显著低于后者(年:2.29±1.20 vs.13.17±5.42,P=0.00),使用情景模拟训练使参加研究的医生技术能力、个人信心及团队配合默契程度均有明显提高(P<0.05),心理压力显著下降(P<0.05),住培组医师接诊患者的心理压力小于非住培组的医师(7.46±0.25 vs.8.43±0.14,P=0.001),在接诊信心上大于后者(8.06±0.18 vs.7.21±0.20,P=0.004).结论 住培医师的培养应从临床工作需求出发,注重岗位胜任力核心内容的训练与提高.情景模拟训练有助于帮助住院医师完成从医学生到临床医生的角色转变.

目的 探讨氢溴酸山莨菪碱(654-1)对室颤导致心脏骤停心肺复苏术后猪冠脉内皮损伤的影响.方法 建立室颤实验动物猪模型,分为三个组:假手术组(S组,n=6)、室颤组(VF组,n=8)和654-1干预组(VF-6组,n=8).在心肺复苏自主循环恢复(ROSC)后0.5 h经股静脉给予654-1(4 mg/kg),分别记录每组实验动物基础状态,ROSC即刻、1、2、4和6 h的舌下灌注血管密度(perfusion vessel density,PVD).ROSC后6 h处死动物,取冠脉前降支组织标本进行HE染色、酶联免疫吸附方法(ELISA)、Western Blot等方法检测,对冠脉血管内皮完整性、上皮细胞形态及冠脉组织syndecan-1、TNF-α水平、半胱氨酸蛋白酶3(Caspase 3)进行比较分析.结果 与S组比较,VF组冠脉HE染色显示冠脉内皮细胞受到破坏,细胞排列不连续、细胞脱落、细胞核浓缩并变形;与S组比较,VF组PVD下降(11.520±3.073 vs.7.642±2.766,P=0.001),冠脉组织syndecan-1(0.387±0.095 vs.1.221±0.238,P=0.009)、TNF-α(0.295±0.290 vs.3.972±1.203,P=0.002)、Caspase 3(139.4±51.2 vs.399.4±128.5,P=0.004)水平明显升高.与VF组比较,VF-6组舌下微循环PVD升高(7.642±2.766 vs.11.350±2.954,P=0.001),冠脉组织syndecan-1(1.221±0.238 vs.0.557±0.279,P=0.004)、TNF-α(3.972±1.203 vs.1.155±0.681,P=0.009)、Caspase 3(399.4±128.5 vs.241.0±61.7,P=0.026)表达水平明显降低.结论 心脏骤停心肺复苏术后,654-1通过改善微循环达到抑制炎症因子释放,保护冠脉内皮糖萼,减少冠脉上皮细胞凋亡,从而保护冠脉内皮功能.

脑损伤被认为是导致心脏骤停自主循环恢复患者死亡的重要原因,挽救、阻止神经细胞损伤,恢复神经功能对于改善复苏预后意义重大.作为心脏骤停患者诊疗中的重要一环,亚低温治疗已经体现出其在神经功能保护方面的优势,但最终使患者获益的不仅仅是体温的降低,还取决于亚低温治疗策略的制定与实施、器官功能的连续评估与监测、必要的辅助支持治疗等集束化诊疗策略的执行.

Objective:To establish a drug-related problems (DRPs) classification system for outpatients and home patients and provide healthcare staff with tools for standardized recording of DRPs.Methods:DRPs classification systems-related literature were collected by searching foreign databases. The selected DRPs classification systems were compared and analyzed, and the initial DRPs classification system was formed by integrating the characteristics of pharmaceutical care for outpatients and home patients in China. The expert consultation form of DRPs classification system was made, expert consultation was performed for 2 rounds using Delphi method, and the final DRPs classification system was formed through modification and adjustment according to expert opinions.Results:A total of 25 DRPs classification systems were obtained, of which 16 were selected and then integrated to form the initial version of the DRPs classification system. The primary structure of the initial DRPs classification system consisted of 5 parts: problem state, problem type, problem cause, problem intervention, and intervention result. Each part had different number of items, among which there were different number of secondary structure items in problem cause, problem intervention, and intervention result. In the first round of consultation, 32 experts were invited and 30 consultation forms were collected, with a recovery rate of 94%. In the second round of consultation, 30 experts were invited and 30 consultation forms were collected, with a recovery rate of 100%. After 2 rounds of consultation, the acceptance rates of experts for the question state, problem type, problem cause, question intervention, and intervention result in the primary structure were 100%, 90%, 83%, 83%, and 97%, respectively. For the evaluation of 5 categories of drug treatment (indications, effectiveness, safety, economy, and compliance) in DRPs classification system, except for that the expert authority coefficient of economics was 0.79, the expert authority coefficient of the other 4 categories of issues was all >0.8. In the first and second round of consultation, the coordination coefficient of expert opinions was 0.386 ( χ2=995.258, P<0.001) and 0.364 ( χ2=971.232, P<0.001), respectively. After modifying with expert opinions, a final version of DRPs classification system was formed, which included 38 primary structure items in 5 categories and 90 secondary structure items in 3 categories. Conclusion:A DRPs classification system for outpatients and home patients suitable for China′s national conditions has been initially established, which can be used as a standardized recording tool for DRP.

目的 观察Glasgow-Blatchford评分系统在经皮冠状动脉介入治疗(PCI)术后双联抗血小板治疗致消化道出血预防护理中的应用效果.方法 选取2019年1月—2019年9月我院心内科收治的急性冠脉综合征行PCI术病人250例,采用随机数字表法分为观察组与对照组,每组125例.对照组围术期实施常规护理与健康指导,观察组在对照组护理基础上给予Glasgow-Blatchford评分系统指导双联抗血小板治疗消化道出血预防护理.观察两组围术期消化道出血的发生率、出血严重程度、出血停止时间、再出血率、住院时间;调查两组消化道出血病人术后服用抗血小板治疗药物的依从率.结果 观察组PCI术后出现消化道出血5例,对照组PCI术后出现消化道出血10例,两组消化道出血发生率比较,差异无统计学意义(P>0.05).观察组PCI术后消化道出血程度明显轻于对照组,差异有统计学意义(P<0.05);观察组消化道出血病人出血停止时间、住院时间均较对照组明显缩短;观察组消化道出血病人随访3个月、6个月时服用抗血小板治疗药物的依从率高于对照组.结论 Glasgow-Blatchford评分系统用于指导PCI术后双联抗血小板治疗消化道出血预防护理,可明显降低消化道出血严重程度,缩短止血时间、住院时间,提高出血病人术后长期抗血小板治疗的依从性.

感染性休克是急诊科常见的急危重症,是指严重感染导致的低血压持续存在,经充分的液体复苏难以纠正的急性循环衰竭,可迅速导致严重组织器官功能损伤,主要死亡原因为多器官功能衰竭,病死率高.我国茄科植物山莨菪中提取得到的抗胆碱药山莨菪碱具有扩张血管、改善微循环、改善心血管功能、保护细胞和降低血乳酸浓度等作用,能降低感染性休克的病死率,是感染性休克治疗的一个重要辅助手段,值得在临床推广应用.本研究通过检索中国知网、万方数据库和维普数据库等数据库,系统收集山莨菪碱治疗感染性休克的相关文献并进行综述.研究结果显示,山莨菪碱用于感染性休克治疗的疗效较好,其抗休克的主要机制包括能够解除血管痉挛、改善微循环、抑制血小板聚集、阻断M胆碱受体、兴奋机体的呼吸中枢、调节迷走神经和抑制腺体分泌等.希望本综述能够对山莨菪碱治疗感染性休克的临床应用提供参考.

目的:探讨前置审核系统对医院门急诊处方质量的影响.方法:收集2017年6-12月首都医科大学宣武医院采用前置审核系统进行门急诊处方点评的资料,与2016年6-12月未采用该系统进行门急诊处方点评的资料进行对比分析.结果:门急诊处方总体合格率在从2016年的96.19％提高至2017年的99.59％;2017年使用前置审核系统后,系统中修改前处方合格率为93.94％,修改后处方合格率为99.93％;医师对该系统的满意度为98.00％.结论:处方前置审核系统顺利开展,提升了门急诊的合理用药水平,有助于药师工作转型.

Objective To investigate the expression of interleukin-4 (IL-4),IL-17,IL-6 and immunoglobulin antibodies in early stage of allergic rhinitis (AR) response and their association with allergic symptoms.Methods At early stage (day 16) and late stage (day 30) of ovalbumin (OVA)-induced allergic rhinitis (AR) mouse model,nasal symptoms were measured;nasal mucosa was collected to detect the gene expression of interferon-γ(IFN-γ),IL-4,IL-17 and IL-6;serum was collected to detect the levels of OVA-specific IgE,IgG1,IgG2a and protein expression of IFN-γ,IL-4,IL-17 and IL-6.Results The number of nasal rubbings and sneezes was increased significantly in late stage compared to early stage and control,and had no significant difference between early stage and control.The level of serum OVA-specific IgE was significantly higher in early stage and late stage than that in control,but has no significant difference between early stage and late stage.Serum OVA-specific IgGland IgG2a,gene expression of IL-17 and IL-6 in nasal mucosa,and serum IL-17 and IL-6 protein had similar expression patterns.These variables were significantly higher in early stage and late stage than those in control,and significantly higher in late stage than those in early stage.Gene expression of IL-4 in nasal mucosa and serum IL-4 protein levels in late stage was significantly higher than those in control.Conclusion IL-17,IL-6,and allergen specific IgG1 and IgG2a may be involved in the early stage of host against allergen response,and their levels may be associated with allergic symptoms.

目的:建立基于信息化辅助的药师实时处方审核模式,并评价该模式的实施效果,为药学服务转型提供支撑.方法:以临床用药决策支持系统为审核规则智库建立信息化审核系统,在医师开具处方/医嘱时进行实时预审.医师对系统审出的问题处方/医嘱进行主动修改,也可以发给专职审核药师进行人工审核.对新模式实施效果进行评价,如处方合格率、医师对系统审核提示的主动修改率和药师审核意见的接受率;医保拒报销费用;医师满意度和患者满意度等指标.结果:该系统上线运行1年内,共审核处方245.49万张,审核医嘱413.19万条.医师对问题处方主动修改率平均为86.48％.经药师实时审核后,处方合格率由95.60％提升到99.67％.医嘱合格率由84.27％提升到97.53％.医师修改前后平均每张处方费用减少(146.25±13.49)元;降脂药拒付金额2017年9月份与2016同期分别为9216.24元和20 540.20元,同比下降55.13％.医师满意度为98.00％;患者对药学服务满意度达到97.30％,比去年同期提高5.70％.结论:基于信息化辅助的药师实时处方审核药学服务模式,可有效提高处方合格率,保证患者用药安全,节省医疗费用.

Objective To investigate the relation between nucleic acid testing of Helicobacter pylori(Hp) and severity of gastric and duodenal benign diseases.Methods From January to June 2018,212 cases of gastric mucosal pathological specimens with positive Helicobacter pylori nucleic acid were collected in Beijing Anzhen Hospital,Capital Medical University.Genotyping and severity of Hp infection were analyzed.Results There were 185 cases(87.3％) with Hp type Ⅰ and 27 cases(12.7％) with Hp type Ⅱ.Rates of chronic non-atrophic gastritis,chronic non-atrophic gastritis with erosion or bile reflux,chronic atrophic gastritis,gastric ulcer,normal duodenum,duodenitis and duodenal ulcer were 20.0％,23.8％,37.3％,18.9％,51.9％,23.8％,24.3％ in the Hp type Ⅰ group and 29.7％,44.4％,22.2％,3.7％,74.1％,22.2％ 3.7％ in the Hp type Ⅱ group,respectively.The types of gastric and duodenal diseases showed significant differences between the Hp type Ⅰ group and Hp type Ⅱ group(P ＜ 0.05).Hp type Ⅰ patients were divided into mild group(17 cases),moderate group(63 cases) and severe group(105 cases).Rates of chronic atrophic gastritis and gastric ulcer in the severe group were higher than those in the mild group and moderate group;rates of chronic non-atrophic gastritis and chronic non-atrophic gastritis with erosion or bile reflux in the severe group were lower than those in the mild group and moderate group(P ＜ 0.05).Conclusions Hp nucleic acid testing has certain clinical significance in judging the severity of gastric and duodenal diseases.Patients with Hp type Ⅰ infection,especially those with severe infection,should undertake Hp eradication therapy.

《父母爱情》电视剧中有这样一个情节,郭涛扮演的海军团长江德福的妹夫在晚年时便秘,因用力排便导致脑出血而死亡. 很多观众反映编剧写得太夸张了,常见的便秘怎么可能导致脑出血呢?其实现实生活中这种情况是会发生的.对于心脑血管患者来说,便秘排便时“用力过猛”,可能会致血管破裂而引发脑出血. 便秘表现为排便次数减少、粪便干硬、排便困难.常见的引起便秘的原因有很多,包括饮食不规律、不良的生活习惯、缺乏运动等.一些药物也会导致便秘,包括神经系统药物、消化系统药物、心血管系统药物等.那便秘要如何治疗呢?

Objective To explore the effect of quality control circle (QCC) on lowering incidence of phlebitis induced by short-term peripheral intravenous infusion of fat emulsion and amino acids (17) and glucose (11％) injection(Calvin) in patients after endoscopic gastric and colonic polypectomy.Methods A prospective study was conducted on patients who had endoscopic gastric and colonic polypectomy from March 2015 to September 2016 in Beijing Anzhen Hospital,Capital Medical University and needed intravenous infusion of Calvin nutrient solution within 3 d after operation;510 patients were enrolled from March 2015 to March 2016 as pre-intervention group;275 patients were enrolled from April to September 2016 as post-intervention group.QCC activity was applied with the theme set as "lowering incidence of phlebitis induced by short-term peripheral intravenous infusion of Calvin nutrient solution";3 root causes of phlebitis-using same venous chamnel between nutrition and medication infusion,not replacing intravenous trocar in 24 h,and too fast intravenous infusion—were derived by the "eightytwenty rule".Incidences of phlebitis before and after application of QCC,target achievement rate and progress rate were analyzed.Results The incidence of phlebitis in post-intervention group was significantly lower than that in pre-intervention group [4.0％ (11/275) vs 13.3 ％ (68/510)] (x2 =8.914,P =0.003).The target achievement rate of QCC activity was 90.3％ and the progress rate was 69.9％.Conclusion Application of QCC helps standardize progress of short-term peripheral intravenous infusion of nutrition;it can lower incidence of phlebitis induced by intravenous infusion and improve professional knowledge and quality management of nurses.

Objective To investigate the effect of quality control circle (QCC) on intestinal cleanliness qualified rate of polyethylene glycol electrolyte powder (Shutaiqing) for colonoscopy in hospitalized patients.Methods Totally 776 hospitalized patients undergoing enteroscopy were enrolled from March 2016 to February 2017 in Beijing Anzhen Hospital,Capital Medical University;480 patients who were investigated from March to September 2016 were pre-activity group;296 patients who were investigated from October 2016 to February 2017 were post-activity group.QCC was established in order to improve the intestinal cleanliness qualified rate of oral taking Shutaiqing for enteroscopy;3 main influence factors of intestinal cleanliness were resolved-patients intolerance of large volume of water intake,simple nursing education and incorrect assess of 3 d pre-enteroscopy diet;countermeasures and plans were developed.The intestinal cleanliness qualified rate before and after QCC activity,target achievement rate and progress rate were analyzed.The impact of QCC on comprehensive abilities of circle members was evaluated.Results The intestinal cleanliness qualified rate in post-activity group was significantly lower than that in pre-activity group[95.6％ (283/296) vs 87.1％ (418/480)] (P ＜ 0.001).The target achievement rate was 93.4％ and the progress rate was 65.9％.Abilities of communication,coordination,brainstorming,team spirit and questions discovery of circle members were improved after QCC activity.Conclusion Application of QCC helps regulate standardized progress of enteroscopy,improve intestinal cleanliness qualified rate in hospitalized patients,and promote professional knowledge,teamwork spirit and quality management ability of nurses.

OBJECTIVE To investigate the expression of SerpinB2 and SerpinB4 genes in cells from the nasal brushings in allergic rhinitis(AR) patients, and their relationships with eosinophil numbers in nasal brushings, serum total IgE level and severity of AR. METHODS Twenty nine control subjects and 59 AR patients[29 specific IgE positive AR(sIgE-P-AR) patients, 30 specific IgE negative self reported AR(sIgE-N-SR-AR) patients] were recruited. The samples of nasal brushings and peripheral blood were collected to detect SerpinB2 and SerpinB4 gene expression and eosinophil numbers in nasal brushings, and total IgE and allergen-specific IgE level in peripheral blood. RESULTS Expression of SerpinB2 and SerpinB4 genes in cells from nasal brushings were significantly higher in sIgE-P-AR [5.17(2.33-18.96), 0.6(0.355-1.08), respectively] and sIgE-N-SR-AR [3.27(1.59-13.4), 0.75(0.42-1.64), respectively] than that in control subjects[1.21(0.1-3.285), 0.29(0.165-0.505)] (sIgE-P-AR:P=0.013; sIgE-N-SR-AR:P=0.002). Expression level of SerpinB2 and SerpinB4 had no relationships with eosinophil numbers in nasal brushings and serum total IgE level. Expression of SerpinB2 in moderate/severe AR[4.74(2.68-47.5)] was significantly higher than that in mild AR[(1.333-5.603)](P=0.025); while expression of SerpinB4 in mild AR[3.95(2.6-7.59)] was significantly higher than that in moderate/ severe AR[2.83(0.715-5)](P=0.042). CONCLUSION SerpinB2 and SerpinB4 genes might be involved in the pathogenesis of AR, and their diagnostic values in AR deserve to be evaluated with larger samples.

Objective To observe the effect of TUG1 gene silencing on glioma cell growth and apoptosis.Methods A slow virus vector pLenti6.3-EGFP of carrying shRNA-TUG1 was constructed and then transfected into human glioma U251 cells.The expression levels of TUG1 LncRNA gene in U251 cells were detected by using the real-time fluorescence quantitative PCR.The inhibitory effect of the interference vector on LncRNA TUG1 gene was observed.Flow cytometry and CCK-8 assay were used to observe the effect of interfering TUG1 gene on U251 cell proliferation and apoptosis.Results The results of real-time fluorescent quantitative PCR detection showed that as for the relative amount of LncRNA TUG1,there were significant differences among the U251 blank control group (0.40 ± 0.05),the negative control group (1.01±0.12),siRNA-1 (0.09±0.01),siRNA-2 (0.32 ±0.01),siRNA-3 (0.18 ±0.04),and siRNA-4 (0.18 ± 0.02) (P ＜ 0.05).The siRNA-1 with the best interference effect was selected for subsequent interference experiments.The apoptotic rate of the TUG1 interference group (11.58 ± 0.11％)was higher than that of the blank control group (8.55 ±0.23％) and the negative control group (9.14 ± 0.17％) (all P ＜0.05);the cell proliferation capacity of the TUG1 interference group (0.64 ±0.23) was lower than that of the blank control group (1.07 ± 0.50) and the negative control group (0.94 ± 0.42) (all P ＜ 0.05).Conclusion TUG1 gene silencing may inhibit the proliferation of U251 glioma cells and promote cell apoptosis.

Objective To observe and analyse the application of isotonic solution nasal irrigation in repair after nasal endoscopic surgery .Methods A total of 375 patients with nasal endoscopic surgery were randomly divided into control group (191 cases) and observation group (184 cases) by random numbers.The patients of control group received routine nursing , while the patients of observation group underwent routine nursing and nasal irrigation .We compared postoperative recovery of nasal mucosa and nasal cavity adhesion of two groups .Results Nasal mucosa recovery of patients in observation group after operation was better than that of control group.Lund-Kennedy endoscopic mucosal pattern grading in control group was (4.62 ±1.45) points, comparing with observation group (3.28 ±0.94) points; sinus CT grading in control group was (5.51 ± 2.36) points, comparing with (4.18 ±1.97) points in observation group (P<0.05).Conclusions Isotonic solution nasal irrigation after nasal endoscopic sinus opening surgery is benefit for the recovery of surgery area , can promote wound healing , and improve the success rate of surgery .

为探讨银黄止痛膏用于肛门病术后镇痛的有效性及安全性,将357例肛门病术后患者随机分为治疗组(179例)和对照组(178例),治疗组换药时应用银黄止痛膏外敷,对照组换药时应用马应龙麝香痔疮膏外敷.对比观察两组镇痛效果、安全性和不良反应.结果显示,在镇痛起效时间、镇痛持续时间、换药后5 min和30 min VAS评分、换药后30 min VAS评分减少率方面,治疗组均优于对照组,P ＜0.05.两组患者用药后血常规、尿常规、肝肾功能均无异常变化,用药过程中均无皮肤过敏、湿疹等不良反应.结果表明,肛门病术后应用银黄止痛膏镇痛效果满意,优于马应龙麝香痔疮膏,而且安全性高,制作简单、成本低廉、应用方便,值得临床推广应用.

OBJECTIVE:To determine GJB2 allelic mutant and estimate probability of hereditary hearing loss in newborn with GJB2 heterozygous mutation in Beijing.METHOD:We performed genetic testing for sequencing of GJB2 gene for searching GJB2 allelic mutant in 915 newborn who received newborn deafness gene screening (GJB2 c. 235delC, GJB2 c. 299_300delAT, GJB2 c. 176191del16, GJB2 c. 35delG) in Beijing Tongren hospital, and the mutation were classified to pathogenic mutation,undefined variant and polymorphism.RESULT:Four hundred (43.72%, 400/915) newborn were detected to carry at least one mutation allele in GJB2. 3 (0.33%, 3/915) newborn had pathogenic mutations (c. 94C>T, c. 380G>T, c. 344T>G); 62 (6.76%, 62/915) newborn carried 14 undefined variant, 36 newborn had c. 109G>A (58.06%, 36/62),13 newborn had c. 368C>A (20.97%,13/62), six (c. 268C>G, c. 282C>T, c. 294G>C, 456C>T, c. 501G>A, c. 587T>C) are novel; 335 (36.61%, 335/915) newborn were polymorphism.CONCLUSION:The probability of hereditary hearing loss is 7.09% in newborn with GJB2 heterozygous mutation in Beijing. It is noteworthy that c. 109G>A, c. 368C>A occupy a high proportion.