OBJECTIVE To explore the correlation and biological significance of the expression levels of microribo-nucleic acid-362(miR-362)and long-chain non coding ribonucleic acid(RNA)small nucleolar RNA host gene 12(LncRNA SNHG12),and the positive rate of interleukin-17(IL-17)methylation with high-risk human papilloma-virus(hr-HPV)infection in patients with cervical cancer.METHODS A total of 168 patients with cervical cancer admitted to the Fifth Central Hospital in Tianjin from Jan 2019 to Aug 2023 were enrolled and divided into the group A;170 patients with cervical intraepithelial neoplasia in the hospital were included in the group B,and 180 female volunteers under health examinations during the same period were in the group C.The clinical data and the occurrence rate of hr-HPV infection were compared among the three groups;the expression levels of cervical tis-sue miR-362 and LncRNA SNHG12 and positivity rate of IL-17 methylation among the three groups and between the hr-HPV-positive and negative cervical tissues were also compared.RESULTS The expression level of miR-362 in cervical tissue of the group A was lower than that of the group B and the group C,and the group B was lower than the group C.The expression level of LncRNA SNHG12 in cervical tissue of the group A was higher than that of the group B and the group C,and the group B was higher than the group C.The positive rates of IL-17 methyl-ation in the three groups were 68.45％,52.35％,and 37.78％,respectively,and the differences among them were statistically significant(P＜0.05).The expression level of miR-362 in hr-HPV-positive CC patients was lower than that of HR-HPV-negative CC patients.The LncRNA expression level of SNHG12 in cervical tissues was higher than that of HR-HPV-negative CC patients.The positive rate of IL-17 methylation was 72.54％,which was higher than 46.15％in the hr-HPV-negative CC patients(P＜0.05).The expression level of miR-362 in cervical tissue of cervical cancer patients was negatively correlated with hr-HPV-positive infection(r=-0.565,P＜0.05),while the expression level of LncRNA SNHG12 and positivity rate of IL-17 methylation in cervical tis-sue were positively correlated with hr-HPV infection(r=0.498,0.512,P＜0.05).The proportion of highly dif-ferentiated tumors in IL-17 methylation positive patients was 60.87％,higher than 39.62％in the IL-17 methyla-tion negative patients(P＜0.05).CONCLUSION The expression levels of miR-362 and LncRNA SNHG12 in cer-vical tissue,as well as the positive rate of IL-17 methylation were closely related to the occurrence of cervical canc-er and hr-HPV infection,which has certain correlation with pathological characteristics of patients in clinic.
Objectives: The goal of this project was to evaluate the degree of contamination of the air and lab surfaces during the extraction of RNA from COVID-19 under laboratory ventilation systems, identify operational risk areas, and provide a reference for reducing medical contamination in COVID-19 detection.Methods: Our COVID-19 testing contains the real-world test and a splash test, detected the RNA residue of COVID-19 pseudovirus on the surface of air and object with PCR.Results: Our results show that RNA extraction contamination occurs more frequently under non-negative pressure than under negative pressure. The biological safety cabinet features a system to stop contamination that may successfully halt the transmission of aerosols as well as the contamination of the experimenter's glove, which is a major source of transmission. Furthermore, UV disinfection cannot completely sterilize an area and regular and effective cleaning is also necessary.Conclusions: In summary,our study lays the foundation for efficacy evaluation of laboratory construction of positive and negative pressure systems and also elucidates the focus of laboratory contamination prevention, providing a strong anti-contamination basis for the extraction of RNA testing for the prevention and control of COVID-19 and even other respiratory transmitted diseases.
目的 探究结直肠癌术后切口感染患者分离产β-内酰胺酶(ESBLs)大肠埃希菌的耐药性及分子分型.方法 收集 2018 年 5 月-2021 年 5 月于中国医科大学附属盛京医院进行手术治疗的结直肠癌患者术后切口感染分泌物分离出的大肠埃希菌 105 株,筛选产 ESBLs大肠埃希菌菌株 48 株,采用纸片扩散(K-B)法进行药敏试验,采用聚合酶链式反应(PCR)扩增鉴定产 ESBLs大肠埃希菌β-内酰胺酶基因型,采用多位点序列分型(MLST)分析产ESBLs大肠埃希菌ST分型.结果 48 株产ESBLs大肠埃希菌对碳青霉烯类抗菌药物完全敏感,对阿米卡星、妥布霉素耐药率分别为 18.75%和 25.00%,但对其他抗菌药物耐药率均较高,表现为多重耐药;产 ESBLs大肠埃希菌对常用抗菌药物(阿米卡星、妥布霉素、亚胺培南和美罗培南除外)的耐药率高于非产 ESBLs 大肠埃希菌(P<0.05);48 株产 ESBLs大肠埃希菌均检出β-内酰胺酶基因,其中 31 株(64.58%)检出携带TEM-1 基因,19 株(39.58%)检出携带CTX-M-14 基因;48 株产 ESBLs大肠埃希菌共有 10 种 ST分型,以 ST131(31.25%)为主.结论 结直肠癌术后切口感染患者分离产 ESBLs大肠埃希菌表现为多重耐药,对多种抗菌药物存在不同程度耐药,产 ESBLs大肠埃希菌以TEM-1、CTX-M-14 为主要基因型,主要 ST分型为 ST131 型.
目的 探究树突状细胞相关C型凝集素受体 2(Dectin-2)基因多态性与乳腺癌根治术后继发侵袭性真菌感染(IFI)的关联.方法 选取 2019 年 1 月-2021 年 6 月于中国医科大学附属盛京医院接受根治术的乳腺癌患者124 例,根据患者术后是否发生 IFI分为 IFI组 39 例和非 IFI组 85 例.调查患者病历档案收集临床资料,分析患者术后发生 IFI的危险因素.采用酶联免疫吸附法检测两组患者血清Dectin-2、白细胞介素-1β(IL-1β)和干扰素-γ(IFN-γ)水平,聚合酶链式反应-限制性片段长度多态性(PCR-EFLP)技术检测 Dectin-2 基因 rs11045418 位点单核苷酸多态性.结果 IFI组合并糖尿病、住院时间≥10 d及进行辅助化疗占比高于非 IFI组(P＜0.05),但两组年龄、体质量指数(BMI)及高血压分布占比的比较,无统计学差异;Logistic回归模型分析显示,合并糖尿病、住院时间是乳腺癌患者根治术后继发 IFI的危险因素(P＜0.05);IFI组患者 Dectin-2、IL-1β和 IFN-γ水平高于非 IFI组(P＜0.05);rs11045418 位点 CC 基因型是乳腺癌患者根治术后继发 IFI 的保护因素(P＜0.05),但两组rs11045418 位点基因型和等位基因分布频数的比较,无统计学差异.结论 Dectin-2 基因 rs11045418 位点 CC基因型可能降低乳腺癌患者根治术后继发 IFI风险,血清Dectin-2 水平在 IFI患者中也异常升高.
目的 探究风险控制模式护理对无菌层流床入住的白血病患儿化疗感染预防的应用效果.方法 选择2019年6月-2022年6月于中国医科大学附属盛京医院接受化疗治疗的急性白血病患儿126例作为研究对象,随机分为研究组和对照组,各63例,对照组采用常规护理,研究组在对照组的基础上采用风险控制模式护理.比较两组患者治疗期间的感染情况、住院时间、抗菌药物使用时间及患儿家属满意度.结果 研究组患儿的感染率为22.22％(14/63)低于对照组(P=0.001);研究组住院时间、抗菌药物使用时间均短于对照组(P＜0.05);研究组满意度优于对照组(P=0.020).结论 风险控制模式护理应用于急性白血病患儿化疗治疗入住无菌层流床,可有效降低患儿的感染率,提高患儿家属的满意度.
