Peri-implantitis,as a common complication after implant restoration,seriously affects the prognosis of the patients and causes a lot of inconvenience to the normal life of the patients,and its pathogene-sis is not entirely clear.In recent years,laser therapy,photodynamic therapy,local sustained-release drug-load-ed materials and many other emerging technologies have been gradually applied in clinical practice,and have played a positive promoting role in the treatment of peri-implantitis.However,the research on the application of local sustained-release materials in peri-implantitis is still in the initial stage,and there is a lack of systemat-ic induction and summary of relevant research literatures.This article reviews the research status quo,treat-ment status quo and application of emerging local sustained-release drug-loaded materials for peri-implantitis by consulting domestic and foreign literatures,so as to provide reference for clinical research and application.
Objective To investigate the effect of the glutathione peroxidase 4(GPx4)on mouse so-matic cell reprogramming.Methods To compare the expressions of GPx4 in OG2 mouse embryonic fibroblast(OG2-MEF)cells(MEFs group)and mouse embryonic stem cells(mESC,mESCs group),the expression lev-el of intracellular GPx4 was determined by transcriptome sequencing technique and Western blot.To verify the effect of GPx4 on the efficiency of the somatic cells reprogramming,the complete open reading frame se-quence of GPx4 gene and its selenocysteine insertion sequence(SECIS)were connected to the retroviral vector pMXs for constructing the overexpressed plasmid pMXs-GPx4.Gpx4-targeting short hairpin RNA(shRNA)was synthesized and connected to pSUPER vector,GPx4 shRNA1 and GPx4 shRNA2 were constructed to knockdown GPx4 expression.The above plasmids were co-transfected with pMXs-Sox2,pMXs-Klf4 and pMXs-Oct4 into MEF cells for reprogramming induction to obtain the pMXs no-load control group(pMXs NC),pMXs GPx4 group,pSUPER no-load control group(pSUPER NC),GPx4 shRNA1 group and GPx4 shRNA2 group.The expressions of GPx4 gene and multifunctional marker genes Rex1,Sox2,Dappa3,Sall4,Oct4 and Nanog were detected by real-time fluorescence quantitative PCR.The induced pluripotent stem cells(iPSC)were detected by immunofluorescence staining;the number of iPSC clones generation was detected by alkaline phosphatase staining of pluripotent stem cells;the GPx4 protein expression was detected by Western blot.Results The mRNA and protein expression of GPx4 in the mESCs group was higher than that in the MEFs group;compared with the pMXs NC group,the expression level of GPx4 mRNA in the pMXs GPx4 group was significantly increased;compared with the pSUPER NC group,the GPx4 mRNA and protein levels in the GPx4 shRNA1 group and GPx4 shRNA2 group were decreased(P＜0.05);the iPSC clone number in the pMXs GPx4 group was higher than that in the pMXs NC group,but the difference was not statistically significant(P＞0.05).The number of iPSC clones in the GPx4 shRNA1 group and GPx4 shRNA2 group was significantly lower than that in the pSUPER NC group,and the difference was statistically significant(P＜0.05).After completing the reprogramming,compared with the original MEF cells,the expression levels of various pluripotent marker genes Rex1,Sox2,Dappa3,Sall4,Oct4 and Nanog in the generated iPSC of each group were increased.Conclusion GPx4 knockdown could inhibit the efficiency of somatic cell reprogram-ming,its generated induced pluripotent stem cells have the normal pluripotent gene expression ability.
Background: It is rare to see knee lumen infection with calf effusion abscess. Traditional open surgery is traumatic, slow and difficult to recover. And there are few reports of using arthroscopy to diagnose and treat knee lumen infection with calf effusion abscess. This case series aims to introduce the clinical outcomes of arthroscopic treatment of knee lumen infection with calf effusion abscess. Methods： fifteen patients with knee joint cavity infections and calf abscesses from from January 2015 to December 2019, were included(11 males and 4 females; age range from 33 to 61, the average age is 46.6years). All patients were treated with arthroscopic multi-channel combined approach. The erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were recorded before and at the last follow-up. The knee function was evaluated according to the visual analogue scoring method (visual analogue scale, VAS) pain score and Lysholm score. Results： fifteen patients were followed up from 12 months to 2 years, and the average follow-up time was 18.4 months. The operation was smooth in all patients, the incisions were healed at grade I, no postoperative recurrence, no nerve damage, and no complications such as knee stiffness. At the last follow-up, ESR decreased from preoperative (44.6±10.1) to (11.4±3.1) (P <0.001), and CRP decreased from preoperative (30.2±8.0) to (3.9±1.0) ( P <0.001). Lysholm score was reduced by Before (46.9±7.7) improved to (93.0±8.1) ( P <0.001), the VAS score decreased from (7.0±0.9) before operation to (2.1±1.2) ( P <0.001), and the differences were statistically significant. Conclusion： Arthroscopic multi-channel combined approach is a safe, effective and minimally invasive treatment method for knee joint cavity infection with calf abscess.
目的 探讨机器人辅助腹腔镜下前列腺癌根治性切除术(RARP)中保留耻骨前列腺韧带(PPLs)是否能使尿控功能得到更好的恢复.方法 回顾性分析该院2016年8月至2021年8月行RARP的107例前列腺癌(PCa)患者的临床资料,包括年龄、基础病史等基线数据,病理分期分级、淋巴转移及周围累及、术后并发症等围术期数据,术后随访的尿控情况及国际尿失禁咨询问卷尿失禁简表(ICIQ-UI SF)评分,其中标准术式组56例(A组),保留PPLs组51例(B组).对2组以上资料进行统计分析.结果 手术均获成功,无中转开放及患者死亡.2组患者基线资料及术中情况相关数据差异无统计学意义(P＞0.05).术后3个月,A组漏尿频率评分明显高于B组[(2.05士1.30)分vs.(1.53±1.14)分,P=0.046],ICIQ-UI SF总分也明显高于B组[(7.80±3.73)分vs.(6.29±3.70)分,P=0.038].术后1年,A组漏尿量评分[0(0,1)分vs.0(0,0)分,P=0.078]、对生活的影响程度[0(0,2)分vs.0(0,0)分,P=0.014]明显高于B组,ICIQ-UI SF总分也明显高于B组[0(0,5)分vs.0(0,0)分,P=0.01].2组出血量与引流管留置时间比较差异无统计学意义(P＞0.05),但B组的手术时间[175(140,229)min vs.160(140,180)min,P=0.074]与住院时间[(16.66士5.08)dvs.(14.51±5.47)d,P=0.037)]较A组更短.结论 保留PPLs的手术方式安全可行,有助于PCa患者更好地恢复尿控功能,并提高远期的生活质量,值得推广.
目的 探讨甘油三酯-葡萄糖指数(TyG)与急性冠状动脉综合征(ACS)患者冠状动脉病变严重程度的关系,分析其对病变严重程度及心血管结局的预测价值.方法 纳入442例行冠状动脉介入治疗的ACS患者,随访20个月,依据TyG分为低TyG组(<9.0)和高TyG组(≥9.0).分析不同TyG组患者基线资料间的关系.logistic回归模型评估TyG与冠状动脉病变SYNTAX评分间的相关性.ROC曲线、Cox风险比例模型分别评价TyG与冠状动脉病变SYNTAX评分和主要心脑血管不良事件(MACEs)的相关性.结果 多因素logistic回归模型表明高TyG组SYNTAX评分较低TyG组增加[OR=3.09,95％CI(1.88,5.07),P<0.05].ROC曲线证实TyG对ACS患者SYNTAX评分及MACEs具有诊断价值[AUC=0.62,95％CI(0.54,0.67),P<0.05;AUC=0.66,95％CI(0.60,0.71),P<0.05].Cox风险比例模型表明TyG是MACEs的独立预测因子[HR=2.03,95％CI(1.29,3.17),P<0.05].结论 TyG越高冠状动脉病变越严重、MACEs发生率越高.
