A novel nanocomposite hydrogel-based Surface-enhanced Raman scattering (SERS) platform was successfully fabricated using Ag NPs/S-CNF/PVA hydrogel as the core matrix, demonstrating exceptional swelling-deswelling behavior. By evenly distributing a large number of Ag NPs on the surface of S-CNF, a one-dimensional tightly arranged "hot spot" was successfully constructed, which significantly improved the SERS effect and improved the sensitivity and uniformity of the SERS platform. The experimental results show that Rhodamine 6G (R6G) of 10-7 M can be detected, RSD = 1.90 %. At the same time, PVA hydrogel, as an excellent packaging material, can effectively isolate Ag NPs/S-CNF/PVA composite from the external environment, which helps to maintain the SERS property of the film, but also makes the film more durable and reliable in practical applications. By using different signal molecule modification, different substances were determined. It is worth mentioning that the substrate showed excellent SERS activity on glucose and pH, which has the potential to be applied in non-invasive tear glucose and pH monitoring system. Given its excellent flexibility and repeatability, the substrate is expected to be integrated into a variety of wearable biosensors, thus advancing the development of biological process research. Overall, by combining a flexible SERS 3D substrate, this approach shows great potential and application prospects in the study of biological processes.

A straightforward analysis strategy based on Surface-Enhanced Raman Spectroscopy (SERS) technology was developed for determining amino acid levels in sweat. Polyvinyl butyral (PVB) is an excellent polymer that is used to coat the surface of sulfonated cellulose silver nanocomposites (S-CNF-Ag NPs) films to improve the mechanical properties and sensing properties of the two-dimensional substrate. The S-CNF-Ag-PVB film showed a significant increase in tensile stress and strain, reaching a stress level of 88.34 MPa, toughness of 5620 kJ/m3, and a 3-fold increase in strain. It also exhibits excellent SERS activity when detecting methylene blue (MB) signal molecules, with a minimum detection of 10−9 M. As an ultrasensitive SERS sensor, S-CNF-Ag-PVB demonstrates excellent sensing properties for tryptophan (Trp), tyrosine (Tyr), arginine (Arg), glutamic acid (Glu), and phenylalanine (Phe) as sweat metabolites. The developed two-dimensional substrate has been effectively utilized for the precise detection of amino acids in sweat.

In this study, a surface-enhanced Raman scattering (SERS) substrate based on oxidized sodium alginate (OSA)-modified silver nanoparticles (Ag NPs) was developed for the highly sensitive detection of urine biomarkers. Compared to conventional citrate-reduced Ag NPs, the OSA-Ag NPs eliminate the need for additional reducing agents, offering cost-effectiveness and alignment with green chemistry principles. OSA acts as both a stabilizer and a binding site for Ag NPs, enabling uniform nanoparticle distribution and enhanced substrate robustness. This modification significantly improves the substrate's high-salinity stability, sensitivity, and reproducibility. Furthermore, the OSA-Ag NPs substrate exhibited remarkable storage stability, maintaining 92.49 % SERS intensity after 50 days under ambient conditions, which outperforms traditional Ag colloid substrates. The OSA-Ag NPs exhibited detection limits as low as 1.9 × 10-4 M for urea, 2.4 × 10-7 M for creatinine, and 2.7 × 10-7 M for bilirubin, demonstrating high sensitivity and a wide linear range. In artificial urine, the OSA-Ag NPs with high accuracy and repeatability, confirming their practical utility. These results highlight the OSA-Ag NPs substrate as a promising tool for urine biomarker detection, particularly in high-salinity environments. Its ability to maintain performance in complex biological matrices suggests potential for rapid and precise diagnostics, advancing the field of biosensing.

Silver nanowire (Ag NW)/gold nanosphere (Au NS) binary plasma films were prepared using plasma coupling between Ag NWs and Au NSs. The plasma films formed by combining these two noble metals showed better sensitivity for SERS detection with a minimum detection concentration of 10−8 M for R6G compared to pure Ag NWs or Au NSs. After rational optimisation of the substrate preparation process, the substrate showed good homogeneity, reproducibility and stability. We perform an indirect detection of homocysteine (Hcy) through a specific reaction of Hcy with o-phthalaldehyde (OPA). The lowest detectable concentration of Hcy was 5 × 10−9 M. The recoveries of Hcy were 94.53 103.43

As a reactive oxygen species (ROS), excessive production of H2O2 contributes to the development of several diseases such as, inflammation, cancer, and respiratory diseases. Supplementation with endogenous or exoge-nous antioxidants can scavenge ROS and reduce the oxidation of cellular molecules, thus alleviating the gen-eration of diseases. Therefore, the determination of H2O2 content and its antioxidant activity is of great importance in disease diagnosis and treatment. In this paper, a ratiometric SERS sensor with a flexible cellulose membrane was designed for quantitative detection of H2O2 and assessment of antioxidant activity. First, gold seeds were reduced on bacterial cellulose membrane (BCM) and Au NPs were smoothly deposited on the bac-terial cellulose membrane (BCM) using halides to reduce the reduction potential in the growth solution to form a flexible BCM@Au NPs SERS substrate. Afterwards, the oxidation of H2O2 was used to convert 3-mercaptophenyl-boronic acid (3-MPBA) to the corresponding phenol form 3-hydroxyphenylethanol (3-HTP). The change of substance resulted in a good linear relationship between the intensity ratio corresponding to the two Raman shifts of 881 cm-1 and 995 cm-1 and the H2O2 concentration with a detection limit of 0.0186 mu M. This opens up a new method for the detection of H2O2 with high sensitivity and accuracy. In addition, this SERS platform was successfully used for the determination of antioxidant activity. It is promising to be applied to disease diagnosis and efficacy evaluation.

Composite magnetic nanoparticles Fe3O4@PB@Au 3 O 4 @PB@Au nano-enzymes were synthesised to combine colorimetry and surface-enhanced Raman spectroscopy (SERS) to construct sensors for sensing H2O2 2 O 2 and glucose detection. Glucose oxidase (GOx) was assembled to Fe3O4@PB@Au 3 O 4 @PB@Au to form Fe3O4@PB@Au@GOx 3 O 4 @PB@Au@GOx integrated nano- enzymes, which catalyse the formation of gluconic acid and H2O2 2 O 2 from glucose. In the presence of H2O2, 2 O 2 , Fe3O4@PB@Au, 3 O 4 @PB@Au, as a peroxidase model enzyme, catalyses the oxidation of 3,3 ',5,5 '-tetramethylbenzidine ' ,5,5 '-tetramethylbenzidine (TMB) to blue charge transfer complex (oxTMB) for color development and as a substrate to facilitate the detection of oxTMB by SERS. In addition, Fe3O4@PB@Au 3 O 4 @PB@Au has good homogeneity, reproducibility, stability and reusability, which ensures the long-term applicability of the sensing system. The limits of detection (LOD) for H2O2 2 O 2 glucose were 13.4 mu M and 0.313 mM in the colorimetric assay; the LODs for H2O2 2 O 2 and glucose in the SERS assay were as low as 38 nM and 2.94 mu M. The nano-enzymes could be successfully applied to serum without pretreatment, and this cross-referencing strategy improved the detection limit of the colorimetric assay and the accuracy of the SERS assay.

针对高校生物实验室目前的安全教育现状,分析了利用微信公众平台开展实验室安全教育的可行性,构建了一种基于微信公众平台的高校生物实验室安全教育模式.该模式打破了时间、空间的限制,激发了学生安全学习的热情,强化了安全意识,提高了安全技能,进一步保障了实验操作中人身、财产安全.从提高微信关注度、优化教育内容、培养微信管理骨干等方面给予实施建议,旨在为高校安全教育管理工作提供切实有效的实践参考.

The first class serves as the main avenue for cultivating chemistry teachers'quality,while the second class also plays an indispensable role.Using the Chemistry Branch of the Future Outstanding Teachers'Association at Fujian Normal University as a case study,this paper evaluates the feasibility of the association as the second classroom to reinforce teachers'quality training of chemistry normal students.This evaluation encompasses three facets:community systems,community activities and community advantages.In conclusion,we suggest that the college and community level expand incentive policies and reinforce the development of related micro-specializations,offering insights for the training of chemistry normal students in other normal universities.

Surface Enhanced Raman Spectroscopy (SERS) is always challenging for the detection of complex samples due to the pre-processing issues. Therefore, there is a need to develop a high-performance SERS platform that can avoid pre-processing problems and simultaneously enrich target molecules. In this work, we encapsulated cationic cellulose nanofibers (CCNF) stabilized Ag NCs in polyacrylamide hydrogels via photo-crosslinking to construct a high-performance SERS platform for rapid separation, concentration, and enrichment without pretreatment. Ag NCs/CCNF/PAAM hydrogels have good swelling and shrinking properties, which are favorable for enriching the substances to be measured and improving the sensitivity of the SERS platform. The limit of detection (LOD) was as low as 9.33 x 10- 11 M for the detection of the dye molecule R6G. The hydrogel SERS platform has excellent selectivity. Its pores can be adjusted to allow the entry of small hydrophilic molecules and the exclusion of large molecules and hydrophobic substances in complex samples. Therefore, we performed the SERS assay for hydrophilic anticancer drugs in the plasma environment, where the hydrogel allowed doxorubicin hydrochloride (DOX) and 6-thioguanine (6-TG) to enter into the interior of the hydrogel, while hydrophobic substances such as plasma proteins and lipids in plasma were excluded from the hydrogel. As a validation, we performed spiking experiments on plasma from five lung cancer patients, and the assay was sensitive and reliable, with recoveries ranging from 89.0% to 111.0%. Based on the above results, Ag NCs/CCNF/PAAM hydrogels are promising for the real-time detection of drugs in clinical blood.

在"双一流"建设背景下,地方高校学科平台建设得到了长足发展.学科平台是加快学科发展、 提高科研实力、 提升人才培养能力的必要支撑,而学科平台管理人员是影响学科平台支撑能力的关键因素之一.通过对地方高校学科平台的阐述,剖析该类管理人员队伍在学科平台中的作用、 现状,以及加强该类管理人员队伍建设的若干思路,探讨研究地方高校学科平台管理人员队伍的创新建设机制,以期为其他地方高校的平台建设提供参考和借鉴.

Practical teaching is an essential teaching link for applied disciplines,and collaborative education of industry-university-research effectively integrates resources such as education and scientific research to collaborate and cultivate talents.The applied chemistry major of Fujian Normal University is supported by industry-university-research projects,with reform measures such as built campus practical teaching platforms and established practical teaching bases.With the support of practical teaching after the reform,students'comprehensive application,practical ability,innovative thinking have been significantly improved.Students directly connected with the needs of enterprises should be cultivated to achieve a win-win situation for both schools and enterprises.

This study aimed to investigate the effect of aquaporin-4 (AQP4) on tau protein aggregation in neurodegeneration and persistent neuroinflammation after cerebral microinfarcts. A model of diffuse ischemic brain injury was established, and adenovirus was injected stereotactically through the lateral ventricle of mice. The water content of the brain tissue was measured. The co-expression of glial fibrillary acidic protein (GFAP) and AQP4 and the aggregation of p-tau and neuronal marker were detected through immunofluorescence double staining. The expression of phosphorylated microtubule-associated protein tau (p-tau, Ser202/Thr205, Thr205, Ser396, Ser404), interleukin(IL)-6, IL-1β, tumor necrosis factor (TNF)-a, growth associated protein43 (GAP43), GFAP, and ionized calcium-binding adapter molecule 1 (Iba1) was detected through Western blot. It was found that the brain water content in the model group was increased and decreased after the AQP4 interference. Compared with the sham group, the expression of GFAP, p-tau, IL-1β, TNF-a, Iba1, and p-tau was increased in the model group (p < 0.05). Compared with the model group, the expression of p-tau, IL-6, IL-1β, TNF-a, GFAP, and Iba1 was decreased after AQP4 interference (p < 0.05). It is indicated that AQP4 positively regulates neurodegeneration and persistent neuroinflammation caused by tau protein aggregation after cerebral microinfarcts.

近年来,高校实验课学生助理的实施制度逐渐完善.实验课对于生物学学生的发展是不可或缺的,在这一助理模式下,适当的运行机制有助于学生、教师和学院得到三赢.福建师范大学生命科学学院多年来实施实验课学生助理制度,在教学质量和学生培养方面均取得了一定成果.结合本学院开展实验课助理制度的经验,探讨生物本科生在这一岗位上的实践与改进建议.

Background. Chlorogenic acid (CGA) is a polyphenolic compound with antioxidant and anti-inflammatory properties. CGA has been shown to improve neuroinflammation. This study is aimed at elucidating the exact mechanism by which CGA reduces neuroinflammation. Methods. Oxygen and glucose deprivation (OGD) was utilized to treat BV2 microglia and HT-22 hippocampal neurons to engineer an in vitro model of hypoxic ischemia reperfusion. The levels of inflammatory factors (IL-1β, IL-6, TNF-α, IL-4, and IL-10) and oxidative stress factors (MDA, SOD, and GSH-PX) in microglia were determined by ELISA kits. The neuron proliferation was assessed by CCK-8 assay, and LDH kit was used to determine LDH release in neurons. The fluorescent dye DCF-DA was employed to measure ROS levels in neurons. Correlation of MIR497HG, miR-29b-3p, and SIRT1/NF-κB in neurons and microglia was determined by qRT-PCR. Expressions of inflammatory proteins (COX2, iNOS), oxidative stress pathways (Nrf2, HO-1), and apoptosis-related proteins (Bcl-2, Bax, caspase3, caspase8, and caspase9) in microglia or neurons were determined by western blot. The interactions between MIR497HG and miR-29b-3p, as well as between miR-29b-3p and SIRT1, were determined by dual luciferase assay and RIP assay. Results. CGA attenuated OGD-mediated inflammation and oxidative stress in microglia and inhibited microglia-mediated neuronal apoptosis. CGA increased the levels of MIR497HG and SIRT1 and suppressed the levels of miR-29b-3p in BV2 and HT-22 cells. MIR497HG knockdown, miR-29b-3p upregulation, and SIRT1 inhibition inhibited CGA-mediated anti-inflammatory and neuronal protective functions. There is a targeting correlation between MIR497HG, miR-29b-3p, and Sirt1. MIR497HG sponges miR-29b-3p to regulate SIRT1 expression in an indirect manner. Conclusion. CGA upregulates MIR497HG to curb miR-29b-3p expression, hence initiating the SIRT1/NF-κB signaling pathway and repressing OGD-elicited inflammation, oxidative stress, and neuron apoptosis.

介绍了开展大健康通识教育的目的和意义.结合国家首批一流课程"生活药学"的建设和实践,提出在大健康通识教育课程中,要紧抓大健康时事热点,修订教学方案;同时积极利用线上和线下相结合的教学模式,从校内走向全国,传播大健康知识.此外,对大健康通识教育课程的建设提出展望.

食品化学实验作为食品科学与工程专业最重要的专业基础课之一,其实验教学对学生科研创新能力、独立思考能力的培养至关重要,传统的教学模式不利于学生科研能力和科学精神的培养,为了适应新时代人才培养的需求,需要对食品化学实验课程进行改革.食品化学实验应对教学实践进行深入研究,从思政融入课堂、拥抱新的教学载体、深度开放合作、建立合理的评定成绩规则和完善保障机制等方面进行改革,通过改革打破传统的实践教学模式,促进食品领域人才培养的思想创新、观点创新、方法技术创新和模式创新.

Herein, a versatile fluorescent bioanalysis platform for sensitive and specific screening of target miRNA (miR-129-2-3p) was innovatively designed by applying target-induced rolling circle amplification (RCA) for efficient signal amplification. Specifically, miR-129-2-3p was used as a ligation template to facilitate its ligation with padlock probes, followed by an RCA reaction in the presence of phi29 DNA polymerase. The dsDNA fragments and products were stained by SYBR Green I and then detected by fluorescence spectrophotometry. As a result, miR-129-2-3p concentrations as low as 50 nM could be detected. Furthermore, the expression of miR-129-2-3p in breast cancer patients was about twice that in healthy people. Therefore, the results indicated that the RCA-based biosensor system could be a valuable platform for miRNA detection in clinical diagnosis and biomedical study.

发酵工程实验是生物工程类专业的基本课程之一.本次课程改革是通过MOOC线上课程学习,借助虚拟仿真实验模拟操作、 实际发酵罐上进行实践操作、 利用中试车间完整实践一个产品发酵全过程.实现了MOOC线上学习、 虚拟仿真练习、 实验室罐体实验、 中试车间产品验证的四位一体高度融合学习过程.课程改革使学生的满意度接近100％,对发酵知识的掌握,对实际操作的规范实践,对专业思想的坚定,都有极大的上升.

破囊壶菌Aurantiochytrium sp.细胞生长、油脂积累及二十二碳六烯酸(docosahexaenoic acid,DHA)合成与培养温度密切相关.该研究在摇瓶中考察了不同温度对破囊壶菌发酵的影响,结果表明,20～35℃的培养温度较利于破囊壶菌细胞的生长,而25～28℃的培养温度利于油脂的积累;虽然15℃培养时不利于菌体生物量积累,但能显著提高DHA占总脂肪酸的含量.基于以上实验结果,进一步在15 L发酵罐中采用了三阶段控温策略:发酵中前期(0～108 h)使用28和25℃的温度培养,以提高菌体生物量及油脂含量;发酵后期(108～120 h)以15℃低温培养促进DHA积累.采用此策略,发酵获得了116.80 g/L的细胞干重,总脂肪酸量达53.63 g/L,DHA产量为27.17 g/L,其中,油脂含量为45.92％,DHA占总油脂比例达50.66％.该研究为高密度发酵破囊壶菌生产DHA的大规模工业化提供了一种可行的方法.

链霉菌是微生物的主要类群,具有特殊的生理生化特征及代谢途径,可以产生大量结构新颖的活性物质,是天然药用活性先导化合物的重要来源.大量的全基因组测序分析表明链霉菌含有丰富的次级代谢产物合成基因簇,但天然来源的菌株由于培养条件限制,其大部分生物合成基因簇保持沉默或表达微弱,产生的次级代谢产物数量与其生物合成基因簇相比明显偏少,一些次级代谢产物丰富的天才菌株未能得到充分利用.文章重点阐述了采用改变培养基和培养条件、添加化学诱导剂(化学表观遗传修饰剂)及共培养等多效性方法激活链霉菌次级代谢产物合成基因在链霉菌次级代谢产物的二次开发上的新进展,为次级代谢产物丰富的链霉菌资源开发和充分挖掘提供了新途径.