目的 观察针刺结合"三步康复法"治疗颞下颌关节紊乱病的临床疗效.方法 收集福建中医药大学附属人民医院就诊的颞下颌关节紊乱病患者90例,采用随机数字表法分为针刺组、手法组、联合组各30例,分别予针刺、"三步康复法"和针刺配合"三步康复法"治疗.隔天1次,3次/周,3周为1个疗程,1个疗程后比较3组疗效、视觉模拟量表(VAS)评分和Fricton颞下颌关节紊乱指数评分指标中颞下颌关节功能障碍指数(DI)、肌肉压痛指数(PI)、颞下颌关节紊乱指数(CMI)的变化情况.结果 ①VAS评分:3组治疗后均较治疗前降低(P<0.01),针刺组和联合组降低程度显著优于手法组(P<0.01);②Fricton颞下颌关节紊乱指数评分指标:3组治疗后DI、PI、CMI均较治疗前降低(P<0.01),联合组和手法组DI降低程度显著高于针刺组(P<0.01),联合组和针刺组PI降低程度显著高于手法组(P<0.05),联合组CMI降低程度显著高于针刺组(P<0.01)和手法组(P<0.05).结论 三种治疗方法在改善颞下颌关节紊乱病的临床症状及功能方面都具有良好疗效,针刺结合"三步康复法"在改善颞下颌功能方面更具优势.

目的 观察在显微镜下应用ProTaper镍钛锉对上颌第一磨牙mb2根管治疗的临床效果.方法 选取医院口腔科收治的牙髓病与根尖周病患者40例为研究对象,临床检查后均发现存在上颌第一磨牙mb2根管,根据治疗方案的差异将患者均分为传统组与改良组,每组20例.传统组接受K锉预备处理,改良组则接受显微镜下Pro-Taper镍钛锉治疗,比较2组患者的根管填充处理情况、疼痛情况以及患者预备根管的具体时间.结果 改良组填充率为100.0％,高于传统组的65.0％ 例(P<0.05);改良组VAS评分为(2.3±0.4)分,低于传统组的(6.2±0.9)分(P<0.01);改良组根管预备时间为(5.4±0.7)min,短于传统组的(12.2±1.4)min(P<0.01).结论 在临床上颌第一磨牙mb2根管具体治疗中,患者接受ProTaper镍钛锉治疗的效果较好,值得临床推广应用.

Objective To explore the effect of interleukin-10 on inflammatory reaction of Kuffer cell following the Pg-LPS stimulation in hyperlipidemia rabbit.Methods 12 New Zealand rabbits were randomly divided into two groups and were fed with normal or high fat diet.The hyperlipidemia model was established 6 weeks later.We isolated and cultured the Kuffer cells (KC).Both normal and hyperlipidemia group were divided into 3 groups:control,Pg-LPS at 1 μg/mL,and Pg-LPS at 0.1 μg/mL IL-10+1 μg/mL.After the treatment for 24 h,NO levels were detected by Griess assay.NF-κB p65 and IκB-α fragment were inspected by Western blot.ROS levels were determined by fluorescence enzymelabelled meter with DCFH-DA as fluorescent probe.Results The levels of NF-κB p65,NO and ROS were higher in hyperlipidemia control group than in normal control group.After being stimulated by Pg-LPS,the expression of inflammatory substances was increased indicating a synergistic effect between hyperlipidemia and Pg-LPS.Under the IL-10 treatment the expression of NO,ROS,NF-κB p65 were decreased,while the expression of IκB-α was increased.IL-10 inhibited the inflammatory reaction induced by Pg-LPS,and the inhibition was more significant in the KC of hyperlipidemia rabbit.Conclusion The KC of hyperlipidemia rabbit is in a relatively activated condition,after being stimulated by Pg-LPS,hyperlipidemia and Pg-LPS present a synergistic effect.IL-10 can inhibit the inflammatory reaction of KC after the Pg-LPS stimulation,and the effect is better for the KC with hyperlipidemia.

The objective of this study was to investigate the effect of hyperlipidemia (HLP) on innate immune responses to lipopolysaccharide (LPS). Male New Zealand white rabbits were fed a normal diet (ND) or a high-fat diet (HFD) for 8 weeks. In vivo, the rabbits were injected intravenously with LPS for 24 h. In vitro, peripheral mononuclear cells were collected and stimulated (or unstimulated) with LPS for 24 h. Assay results were analyzed with one-way ANOVA or an equivalent non-parametric test. A P value of 0.05 was considered statistically significant. Despite having no influence in body weight, the HFD intake significantly increased serum lipids, C-reactive protein (CRP), nuclear factor (NF)-κB subunit p65, Toll-like receptor (TLR)-4, SR-A and FAS. Although we found increased circulating tumor necrosis factor (TNF)-α, interleukin (IL)-6, CRP, IL-1β, and IL-10 in the ND-fed rabbits, no significant difference was found in the LPS-stimulated production of TNF-α, IL-6, and CRP in the HFD-fed rabbits. The macrophages harvested from the HFD-fed rabbits developed a blunted inflammatory response, with lower mRNA expression of TNF-α, IL-6, CRP, TLR-4, SR-A, FAS, and Bcl-2 than that expressed by the ND group. In the HFD-fed animals, LPS incubation decreased NF-κB subunit p65 expression, whereas the cytoplasmic phosphorylation of the inhibitor of NF-κB protein was enhanced. These data indicate that HLP displayed a form of innate immune paralysis, including reduced pro- and anti-inflammatory cytokine release to external stimulus, which was related to the altered TLR-NF-κB signaling pathway and altered pro- and anti-apoptotic processes in macrophages.

Objective: To explore the effect of interleukin-10 on apoptosis of kuffer cell after the Pg-LPS stimulated in rabbit.Methods: The Kuffer cells were isolated and cultured,and randomly divided into three groups: control group: RPMI1640+Kuffer cell;Pg-LPS group: 1μg/ml Pg-LPS+ Kuffer cell;IL-10+Pg-LPS group: 0.1μg/ml IL-10+1μg/ml Pg-LPS+ Kuffer cell.Cells were collected and real time-PCR was used to detect the mRNA expression of Caspase-3,Fas and p53 after 24 hours.Results: 1μg/ml Pg-LPS induced apoptosis of Kuffer cell and up-regulated the expression of Caspase-3,Fas,p53(P0.05).IL-10 treatment inhibited the apoptosis of Kuffer cell,and reduced the expression of Caspase-3 and Fas(P0.05),but there was no significantly effect on p53 expression.Conclusion: Pg-LPS can induce the apoptosis of Kuffer cell.The mechanism seemed related to the death receptor pathway and mitochondrial pathway.And IL-10 could inhibit Kuffer cell apoptosis through the death receptor pathway.

In order to compare the effect of Porphyromonas gingivalis lipopolysacchride on the inflammatory factors(interlenkin-1β,interlenkin-6,and tumor necrosis factor-α) in monocytes/macrophages of different parts from the New Zealand rabbits,we isolated peripheral mononuclear cells,alveolar macrophages,peritoneal macrophages and kupffer cells.Then the cells from each part were stimulated with E.coli-LPS and Pg-LPS,subsequently the mRNA expressions of IL-1β,IL-6 and TNF-α were analyzed through RT-PCR.The monocytes/macrophages of different parts showed distinct response of inflammatory factors in all groups(P﹤0.05).We also found that compared with the control,E.coli-LPS and Pg-LPS induced higher mRNA expression of inflammatory factors(IL-1β,IL-6,TNF-α,P﹤0.05),but the intensity of E.coli-LPS group was more significant than Pg-LPS group(P﹤0.05).In conclusion,Pg-LPS-activated monocytes/macrophages show site-specific reaction.Periodontal pathogen Pg-LPS could increase the gene levels of inflammatory factors in monocytes/macrophages.

目的:建立兔不同部位单核/巨噬细胞(Mφ)的分离、培养和鉴定的方法,并在体外培养情况下研究其形态、生长特性及生理功能的差异。方法:6只健康新西兰兔以Fi-coll-Hypaque密度梯度离心法分离外周血单核细胞(Mo),以灌洗法分离肺泡巨噬细胞(AM)和腹腔巨噬细胞(PM),以酶消化法+Percoll密度梯度法分离肝脏枯否氏细胞(KC)。在光镜和透射电镜下观察形态学及微观结构的差异,采用墨汁吞噬试验及免疫细胞化学方法分别测定吞噬功能和表型的表达情况。结果:同一个体来源的4个部位贴壁细胞具有单核/巨噬细胞的形态学特征及免疫表型,有较强的吞噬能力,且表现出一定的差异性。墨汁吞噬功能:AM>PM、KC>Mo,MAC387表达:KC>AM>PM>Mo。结论:同一个体不同部位兔单核/巨噬细胞的功能及表型存在异质性。