OBJECTIVE:Little data exists with respect to the relationship between the level of plasma D-dimer and prognosis of small cell lung cancer (SCLC).The aim of this study was to investigate whether the levels of plasma D-dimer could be served as a prognostic factor in patients with SCLC. METHODS:A total of 393 patients with SCLC were addressed in the present retrospective study. Plasma D-dimer levels were measured by immunoturbidimetric assay. The correlation between plasma D-dimer levels and other clinical features, progression free survival (PFS) and overall survival (OS) was analyzed statistically. RESULTS:The plasma D-dimer levels were significantly correlated with karnofsky performance status (KPS), tumor stage, number of metastatic sites, and treatment response. The PFS and OS of patients with elevated D-dimer levels before chemotherapy were significantly shorter than that of patients with normal D-dimer levels (PFS: 6.2 months versus 9.6 months, P<0.001; OS: 15.7 months versus 24.4 months, P<0.001). The patients with D-dimer levels converting from high to normal had better PFS and OS than those with D-dimer levels remaining high after two cycles of chemotherapy. According to multivariate analysis, elevated D-dimer level was confirmed to be an independent prognostic factor for worse survival. CONCLUSIONS:Elevated plasma D-dimer level could be served as an independent determinant of poor prognosis in patients with SCLC.

目的 探究贝伐珠单抗对结直肠癌细胞串珠素表达的影响.方法 将处于对数生长期的肿瘤细胞(HCT116和HT29细胞)分为两组:对照组和实验组(加入终浓度为400 μg/L的贝伐珠单抗48小时),采用RT-PCR检测结直肠癌细胞串珠素mRNA表达.将同一批处于对数生长期的肿瘤细胞分为6组,一组为对照组,其余5组实验组分别加入终浓度为25、50、100、200和400 μg/L的贝伐珠单抗,48小时后ELISA检测直肠癌细胞及培养液上清的串珠素蛋白水平,Western blot检测结直肠癌细胞串珠素的蛋白表达.结果 与对照组比较,实验组肿瘤细胞串珠素mRNA及蛋白水平均上调(均P＜0.05).在细胞培养液中,与对照组比较,实验组的串珠素蛋白水平未出现显著变化(P＞0.05).结论 贝伐珠单抗引起结直肠癌细胞串珠素mRNA和蛋白表达的上调,但未引起细胞培养液巾串珠素蛋白的显著变化.

[目的]探讨贝伐珠单抗对结直肠癌细胞血管内皮生长因子(VEGF)相关受体的影响.[方法] RT-PCR、Western blotting检测不同浓度贝伐珠单抗对结直肠癌肿瘤细胞VEGF相关受体表达水平的影响.[结果]与对照组相比,贝伐珠单抗药物组结直肠癌细胞的VEGF表达水平均明显下降(P＜0.05);VEGFR1和VEGFR2表达水均明显上调(P＜0.05).[结论]贝伐珠单抗可引起了结直肠癌细胞VEGFR1和VEGFR2表达上调.

目的 比较贝伐单抗联合培美曲塞加顺铂(Avastin+ PP)化疗方案与单用培美曲塞加顺铂(PP)化疗方案治疗晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)的临床疗效及不良反应.方法 收集2009年9月-2013年6月在我院应用贝伐单抗联合培美曲塞加顺铂治疗的晚期非小细胞肺癌患者32例为联合治疗组,取同时期、同TNM分期、同病理类型,采用培美曲塞加顺铂化疗方案的患者35例为对照组,所有患者均符合化疗要求.对照组:培美曲塞500 mg/m2,静脉滴注,d1;顺铂75 mg/m2静脉滴注,d1或d1 ～ d3;每21d为1个周期.联合治疗组:培美曲塞和顺铂用法及用量同对照组,贝伐单抗7.5 mg/kg静脉滴注,d1,每21d为1个周期.结果 联合治疗组和对照组疾病控制率分别为90.6％和60.0％(P＜0.05);客观有效率分别为56.9％和25.7％(P＜0.05);中位无进展生存期分别为7.3个月和4.2个月(P＜0.05);中位总生存期分别为20.7个月和9.0个月(P＜0.05),不良反应有骨髓抑制、恶心呕吐、肝肾功能损伤等,但两组差异无统计学意义.结论 贝伐单抗联合培美曲塞加顺铂化疗方案治疗晚期非小细胞肺癌在远期疗效和近期疗效方面均优于单用培美曲塞加顺铂化疗方案,且没有增加严重不良反应.

Objective: To retrospectively review the clinical characteristics and analyze the prognostic factors of Chinese patients with pulmonary neuroendocrine tumors. Materials and Methods: The clinical data of 176 patients with pulmonary neuroendocrine tumors in Chinese PLA General Hospital from Mar., 2000 to Oct., 2012 were retrospectively analyzed. The parameters were evaluated by univariate and multivariate analysis, including the gender, age, smoking history, family history, TNM staging, localization (central or peripheral), tumor size, nodal status, histological subtype and treatment (operation or non-operation). Results: There were 23 patients with typical carcinoids (TC) (13.1%), 41 with atypical carcinoids (AC) (23.3%), 10 with large cell neuroendocrine carcinoma (LCNEC) (5.7%) and 102 with small cell lung cancer (SCLC) (57.9%). The median follow-up time was 64.5 months for AC, 38 months for LCNEC and 27 months for SCLC. The typical carcinoid censored data was 18 (more than 50% of the patients), so the median follow-up time was not obtained, and actuarial 5-year survivals for TC, AC, LCNEC and SCLC were 75.1%, 51.7%, 26.7% and 38.8%, respectively. COX univariate analysis revealed that the age (P=0.001), histological subtype (P=0.005), nodal status (P=0.000), treatment (P=0.000) and TNM staging (P=0.000) were the prognostic factors of the patients with pulmonary neuroendocrine tumors, whereas its multivariate analysis showed that only the age(P=0.001), TNM staging (P=0.002) and treatment (P=0.000) were independent prognostic factors. Conclusions: Radical surgery remains the treatment of choice, and is the only curative option. The age, TNM staging and treatment are confirmed to be the independent prognostic factors in multivariable models for pulmonary neuroendocrine tumors.

OBJECTIVES:To retrospectively review the safety and clinical efficacy of bevacizumab concomitant with chemotherapy in Chinese patients with advanced non-squamous non-small cell lung cancer (NSNSCLC).METHODS:Clinical data for 79 patients with NSNSCLC who received bevacizumab concomitant with chemotherapy in Chinese PLA General Hospital from April 28th 2009 to May 5th 2013 were retrospectively reviewed to analyze the clinical efficacy including disease control rate (DCR), overall response rate (ORR), progression-free survival (PFS), overall survival (OS), the Eastern Cooperative Oncology Group (ECOG) score and the safety.RESULTS:The Eastern Cooperative Oncology Group (ECOG) score was 0-2. By the final cutoff date (June 9, 2013), 54 (68.4%) patients had disease progression and 37 (46.8%) died. The ORR was 32.9% and the DCR was 83.5%. The ORR of the first-, second-, and third- or later-line treatments were 51.4%, 25.0% and 12.5%, while the DCR were 94.3%, 80.0% and 70.8%, respectively. The median OS (mOS) and PFS (mPFS) were 13.5 and 5.83 months, respectively. The mOS of patients with the first-, second-, and third- or later-line treatments were 16.2, 10.9 and 8.30 months, while the mPFS were 7.27, 5.90 and 5.17 months, respectively. Chemotherapy-related adverse events included myelosuppression, vomiting, hepatic dysfunction and renal dysfunction, while the common serious bevacizumab-related adverse events were thromboembolic problems, gastrointestinal perforation and reversible posterior leukoencephalopathy syndrome, which could be well managed.CONCLUSIONS:Bevacizumab concomitant with chemotherapy is effective and the related toxicity can be well tolerated in Chinese patients with NSNSCLC.

Objective To study the safety and effect of rAd-p53 in patients with malignant pleuroperitoneal effusion. Methods Clinical data about 34 tumor patients treated with intra-cavity rAd-p53 infusion in our hospital from October 2007 to April 2012 were retrospectively analyzed. Pleural effusion was treated by infusion of 2×1012 VP diluted into 100 ml 0.9%NaCl while peritoneal effusion was treated by infusion of 4×1012 VP diluted into 500 ml 0.9% NaCl, once 5-7 days for 3 weeks. Their therapeutic effects were assessed according to the WHO criteria and RECIST, respectively. Adverse reactions were assessed according to the NCI CTCAE 4.0. Results Of the 34 patients, 5 (4 with pleural effusion and 1 with peritoneal effusion) were completely improved, and 11 (8 pleural effusion and 3 peritoneal effusion) were partially improved with an overall effective rate of 47.06%. The adverse reactions were mild with grade 1-2 self-limited fever occurred in 19 patients and grade 1 gastric bleeding occurred in 1 patient. Conclusion The effect of rAd-p53 infusion is rather good on pleuroperitoneal effusion and can thus be used as an accessory therapy for advanced tumor patients.

OBJECTIVE:To explore the expression and significance of tumor specific growth factor (TSGF), carcinoembryonic antigen (CEA) and alpha fetoprotein (AFP) in cancer tissue and serum of patients with colon cancer. MATERIALS AND METHODS:Radical surgery for colon cancer was performed on 43 patients with laparoscope under conditions of general anesthesia. The Elisa method was used to detect the levels of serum TSGF, CEA and AFP before and after radical operation, and cancer tissue underwent TSGF, CEA and AFP immunohistochemistry staining after laparoscopic surgery. The decreased conditions of serum TSGF, CEA and AFP in patients with colon cancer at different levels of differentiation and clinical stagings were analyzed, and the relationships of expression rates between histological types, colon cancer morphology, lymph node metastasis and TSGF, CEA as well as AFP in cancer tissue were assessed. RESULTS:Compared with before radical surgery, the levels of serum TSGF, CEA and AFP decreased notably in patients after operations (p<0.01). The decreased degree of TSGF and CEA was the largest in patients with poorly differentiated cancer tissue (p<0.01), while that of AFP was noted in patients with moderately differentiated cancer tissue (p<0.01). The decreased degree of TSGF and AFP was the largest in patients at phase Dukes A (p<0.01), while that of CEA in patients at phase Dukes C (p<0.01). There were no significant differences among the positive expression rates of TSGF, CEA and AFP with different histological types and colon cancer morphologies (p>0.05). The positive expression rates of TSGF and CEA in patients with lymph node metastasis were significantly higher than those without lymph node metastasis (p<0.01). CONCLUSIONS:TSGF, CEA and AFP can be used to evaluate the effect of radical operation for colon cancer, and the changed levels of different markers are associated with tumor differentiation, clinical stating and presence or absence of lymph node metastasis.

Objective To investigate the clinical characteristics of pseudomyxoma peritonei,analyze survival and identify prognostic factors.Method A total of 39 patients treated at our hospital between 2002 and 2011 were identified.A retrospective study was conducted to demonstrate the prognostic factors with univariate and multivariate analyses.Result All patients received operation and 7 patients received intraoperative hyperthermic intraperitoneal chemotherapy(HIPEC) after operation.The median follow up was 40 months.There were 9 deaths in this period,1 of them received HIPEC.The 5-and 10-year survival rates were 89.0% and 35.0%,respectively.The medians of overall survival time was 37 months.Univariate analyses revealed pathologic type and preoperative level of tumor markers did exert an impact on overall survival time(P=0.048,P=0.027).The multivariate analysis identified pathologic type(P=0.033)as an independent prognostic influence on survival.Conclusion The pathologic subtype remains the dominant factor for survival.Normal baseline level with tumor markers seems to have a better survival result.

Background and objective Once the malignant pleural or peritoneal effusion is developed it is difficult to control. This report presents a new method for controlling the malignant effusions. Methods Forty-eight patients, 29 males and 19 females with an average age of 61.2 years old, who were satisfied with the study inclusion criteria, were recruited in this study. Twenty-seven and 21 patients had a malignant pleural and peritoneal effusion, respectively. After draining most of fluids, these patients received intra-cavity infusion of rAd-p53 once per week for 4 weeks, at dose of 2×1012 viral particles (VP) diluted into 200 mL of saline solution for pleural effusions, and 4×1012 VP diluted into 500 mL of saline solution for peritoneal effusions. Results Participants were followed up for a median time of 13.6 month. A total of 11 cases, 7 with pleural effusions and 4 with peritoneal effusions achieved a complete response (CR), and 20 cases (12 pleural effusions and 8 peritoneal effusions) had a partial response (PR). The overall response rate is 64.6%. Patients’ quality of life, assessed by using Karnofsky performance scale (KPS) scores, was improved by an average of 26.4. The one-year of overall survival rate was 54.2% with a median survival time of 12.5 months. There were no serious side effects observed except for self-limited fever found in 79.8% of the cases. Conclusions Intra-cavity infusion of rAd-p53 is an effective and safe treatment for the patients with malignant pleural or peritoneal effusions, especially for those patients who can’t tolerate the standard treatments.

PURPOSE: Gemcitabine, a third-generation anticancer agent, has been shown to be active in several solid tumors. High-grade hemorrhage (grade ≥ 3) has been reported with this drug, although the overall risk remains unclear. We conducted a meta-analysis of randomized controlled trials evaluating the incidence and risk of high-grade hemorrhage associated with gemcitabine. METHODS: Pubmed was searched for articles published from January 1, 1990 to December 31, 2012. Eligible studies included prospective randomized controlled phase II and III trials evaluating gemcitabine-based vs non-gemcitabine-based therapy in patients with solid tumors. Data on high-grade hemorrhage were extracted. Overall incidence rates, relative risk (RR), and 95% confidence intervals (CI) were calculated employing fixed- or random-effects models depending on the heterogeneity of included trials. RESULTS: A total of 6433 patients from 20 trials were included. Among patients treated with gemcitabine-based chemotherapy, the overall incidence of high-grade hemorrhage was 1.7% (95%CI: 0.9-3.1%), and the RR of high-grade hemorrhage was 2.727 (95%CI: 1.581-4.702, p<0.001). Exploratory subgroup analysis revealed the highest RR of hemorrhage in non-small-cell lung cancer (NSCLC) patients (RR: 3.234; 95%CI, 1.678-6.233; p<0.001), phase II trials (RR 7.053, 95%CI: 1.591-31.27; p = 0.01), trials reported during 2006-2012 (RR: 3.750; 95%CI: 1.735-8.108, p<0.001) and gemcitabine used as single agent (RR 7.48; 95%CI: 0.78-71.92, p = 0.081). CONCLUSION: Gemcitabine is associated with a significant increase risk of high-grade hemorrhage in patients with solid tumors when compared with non-gemcitabine-based therapy.