目的 探讨miR-449b对老年原发性肝细胞癌(HCC)患者预后的影响.方法 选取2013年1月-2015年1月于徐州医科大学附属医院肿瘤科接受肝癌根治性切除术治疗的老年HCC患者176例.应用实时荧光聚合酶链反应检测miR-449b在患者癌组织中的表达水平,随访患者的生存状况至2020年1月,评估miR-449b对HCC预后的预测值,分析影响老年HCC患者预后的因素.结果 176例患者年龄66～82岁,平均(70.3±2.7)岁,其中男性141例、女性35例.截至2020年1月,随访的168例患者中,死亡97例.MiR-449b预测老年HCC患者死亡的临界值为0.36.当miR-449b为0.36时,miR-449b预测老年HCC患者死亡的敏感度为72.23％,特异度为70.38％,曲线下面积为0.712(P=0.001).Kaplan-Meier和 Log-rank检验显示,蔡尔德-皮尤改良(CTP)评分B级(HR=1.17,P＜0.001)、肿瘤直径＞5cm(HR=1.13,P＜0.001)、淋巴结转移(HR=1.09,P＜0.001)和 miR-449b低表达(HR=2.54,P＜0.001)会增加老年HCC患者的死亡风险.Cox比例风险回归模型显示,CTP评分B级(HR=1.14,P＜0.001)、肿瘤直径＞5 cm(HR=1.11,P＜0.001)、淋巴结转移(HR=1.07,P＜0.001)和 miR-449b 低表达(HR=2.48,P＜0.001)是老年HCC患者死亡风险增加的独立危险因素.结论 MiR-449b表达与老年HCC患者预后相关,miR-449b表达下调可能增加老年HCC患者的死亡风险.

目的 探讨肺部肿瘤治疗患者心理护理方法和效果.方法 选取我院2011年4月～2015年10月收治的肺部肿瘤患者80例作为本次研究的对象,随机将患者分为心理护理组(40例)和一般护理组(40例),给予心理护理组患者加强心理护理干预,一般护理组患者则按照常规护理模式开展护理,对两组患者的护理满意度情况进行对比分析.结果 心理护理组患者的护理满意度明显高于一般护理组,差异有统计学意义(P＜0.05).结论 肺部肿瘤治疗患者加强心理护理干预具有良好的效果.

Objective To detect the methylation of CpG island in the promoter region of BRCA 1 gene and the level of BRCA1 protein in gastric cancer tissues , and to investigate the relationship between the methylation of CpG island in the promoter region of BRCA1 gene and BRCA1 protein level and its significance .Methods The methylation of CpG is-land in the promoter region of BRCA 1 gene was determined by methylation -specific polymerase chain reaction ( MSP) . The levels of BRCA1 were examined by immunohistochemistry S -P method.Results ① The total methylation rate of CpG island in the promoter region of BRCA1 gene was 48.6%(18/37) in gastric cancer tissues, which was significantly higher than 5.4%(2/37) in adjacent non -cancerous tissues (P<0.05).The methylation of the CpG island was not found in 6 normal gastric tissues .② The methylation rate of CpG island in the promoter region of BRCA 1 gene was 37.8%(14/37) in gastric cancer tissues, which was significantly lower than 70.3%(26/37) in adjacent non -cancer-ous tissues (P<0.05).The expression of BRCA1 protein was detected in 6 normal gastric tissues.③According to the Spearman's correlation analysis , the methylation of CpG island in the promoter region of BRCA 1 gene and BRCA1 protein expression was negatively correlated .Conclusions A high proportion of CpG island in the promoter region of BRCA 1 gene is methylated in gastric cancer tissues .The methylation of CpG island in the promoter region of BRCA 1 gene is neg-atively correlated with BRCA 1 protein level .The methylation of the CpG island may be an important reason for the loss of BRCA1 protein expression .

Objective To investigate the effects of lentinan on the cellular immune function of patients with ad-vanced gastric cancer during perichemotherapy .Methods 62 patients diagnosed with advanced gastric cancer by patho-logical and cytological tests were randomized into groups A and B:group A ( 35 cases ) were treated with lentinan com-bined with DCF regimen ( docetaxel , cisplatin and 5-fluorouracil ) and group B ( 27 cases ) received DCF regimen a-lone.After chemotherapy, the activities of T lymphocyte subgroups and natural kill (NK) cells were measured.Results In group A, the levels of CD3, CD4, CD4 /CD8 and NK cells were obviously elevated after treatment .The amounts of CD8 was remarkably reduced when compared with the pre -treatment level (P<0.05).An opposite trend was observed in the control group (P<0.05).The levels of CD3, CD4, CD4 /CD8 and NK cells were significantly higher in group A than in group B (P<0.05).Conclusion Lentinan can remarkably enhance the cellular immune function of patients with advanced gastric cancer during perichemotherapy .

Objective To investigate the relationship between the methylation of breast cancer susceptibility gene 1(BRCA1)promoter CpG island and the expression level of BRCA1 mRNA in gastric cancer and the signifi-cance. Methods Methylation-specific PCR was used to detect the methylation of BRCA1 gene promoter CpG island in the tumor tissues and paired adjacent cancerous tissues from 37 patients gastric cancer and the 6 normal gastric tissues. reverse transcription PCR was performed to detect the expression of BRCA1 mRNA in the tumor tissues and paired ad-jacent cancerous tissues from 37 patients gastric cancer and the 6 normal gastric tissues. Results The methylation rate of the BRCA1 gene promoter CpG island in the gastric cancer tissues[48. 6%(18/37)]was higher than those in the paired adjacent tissues[5. 4%(2/37)]and the normal gastric tissues[0. 0%(0/6)](χ2 =17. 541,5. 021;P<0. 05). The expression positive rate of the BRCA1 mRNA in the gastric cancer tissues[48. 6%(18/37)]was higher than those in the paired adjacent tissues[94. 6%(35/37)]and the normal gastric tissues[100. 0%(6/6)](χ2 =19. 215,5. 520;P <0. 05 ). The methylation of BRCA1 gene promoter CpG island has a negative correlation with BRCA1 mRNA(r= -0. 515,P<0. 05). Conclusion Methylation of BRCA1 gene promoter CpG island may be one of the main reasons for the loss of BRCA1 mRNA expression.

目的 探讨大蒜素对K562细胞株及裸鼠移植瘤的作用.方法 利用MTT法检测不同浓度大蒜素对K562细胞的增殖抑制影响;Annexin V-FITC标记法检测K562细胞凋亡;建立K562细胞Balb/c裸鼠皮下移植瘤模型,荷瘤小鼠随机分成4组,分别用1.5、3、6 mg/ml大蒜素和生理盐水对瘤体进行局部注射,每次0.1 ml,隔日1次,连续7次,观察瘤体变化.结果 不同浓度大蒜素对K562细胞均有抑制作用,呈明显的剂量依赖性,抑制率分别为21.5%、55.1%、81.6%(P＜0.05);0.005 mg/ml大蒜素诱导K562细胞凋亡作用最显著,0.025 mg/ml大蒜素使K562细胞呈中毒反应,凋亡作用不明显;大蒜素组瘤体生长较对照组明显受到抑制,抑瘤率分别为18.5%、79.2%、91.6%.结论 大蒜素具有显著抑制K562细胞增殖及促凋亡的作用,能有效抑制K562细胞Balb/c裸鼠移植瘤的生长.

Objective To construct a recombinant a recombinant human inhibitor of growth 4 adenovirus vector. Methods Using site-specific mutagenesis technique,mING4 gene was changed into hING4 gene after fourth cycle PCR. hING4 cDNA was introduced into the shuttle plasmid pAdTrack-CMV,the recombinant shuttle plasmid(pAdTrack-CMV-ING4)and the backbone plasmid(pAdEasy-1) were linealized with PmeI digestion and then under co-transformation in bacteria E.coil BJ5183.Then,the newly recombinant plasmid pAdEasy-1-pAdTrack-CMV-ING4 was linearized with Pac I and was transferred into QBI-293A cells to form Ad-ING4. The control virus Ad-null with GFP was constructed in the same manner.The recombinant adenoviruses were amplified in QBI-293A cells,and viruses were purified from these cells to obtain viral stock. Results Sequencing and RT-PCR results proved that recombinant hING4 gene was constructed successfully. Conclusion The recombinant adenovirus hING4 vector was constructed successfully.