Following the publication of this paper, it was drawn to the Editor's attention by a concerned reader that the cellular images shown in Fig. 7A on p. 995 were strikingly similar to data in a paper that had previously been published in the journal Molecular Cancer that was written by different authors at different research institutes. Furthermore, an independent analysis of the data in this paper performed by the Editorial Office revealed that flow cytometric data in Fig. 2A, the cell migration and invasion assay data in Fig. 3A and D and the control western blot data in Fig. 5A were also strikingly similar to data that had either previously been published in articles written by different authors at different research institutes, or which were submitted for publication at around the same time. Given that the abovementioned data had already been published before the receipt of this paper at International Journal of Molecular Medicine, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused. [International Journal of Molecular Medicine 42: 988‑997, 2018; DOI: 10.3892/ijmm.2018.3685].

The prevalence of Peripheral Artery Disease (PAD) is rising globally, yet early risk stratification remains challenging due to the limitations of traditional obesity metrics. TyG–ABSI, an index combining Triglyceride-Glucose (TyG) with A Body Shape Index (ABSI), is a novel marker reflecting both functional insulin resistance and structural visceral adiposity. However, its predictive value for PAD remains unexplored in large prospective cohorts. We included 390,274 adults from the UK Biobank. Baseline characteristics were analyzed across TyG–ABSI quartiles and PAD status. Associations between TyG-related indices and incident PAD were assessed using multivariable-adjusted Cox regression, Kaplan–Meier survival curves, and restricted cubic splines. Robustness was evaluated via Fine-Gray competing risk models, propensity score matching, subgroup analyses, and external validation in the NHANES database. Consensus k-means clustering, integrating biochemical and insulin resistance markers, identified metabolic phenotypes and stratified PAD risk. Feature selection (LASSO, Boruta, and Minimum Redundancy Maximum Relevance [mRMR]) guided the development of six machine learning models (logistic regression, GBM, XGBoost, AdaBoost, LightGBM, and neural network) for PAD prediction, with interpretability assessed via SHAP analysis. Higher TyG–ABSI and related indices were strongly associated with increased PAD incidence (cumulative incidence at 15 years: 4.16

Background: Peripheral arterial disease (PAD) is a common manifestation of atherosclerosis, affecting over 200 million people worldwide. The incidence of PAD is increasing due to the aging population. Common risk factors include smoking, diabetes, and hyperlipidemia, but its exact pathogenesis remains unclear. Nutritional intake is associated with the onset and progression of PAD, although relevant studies remain limited. Some studies suggest that certain nutritional elements may influence the development of PAD. This study aims to explore the relationship between nutrition and PAD using machine learning techniques. Unlike traditional statistical methods, machine learning can effectively capture complex, nonlinear relationships, providing a more comprehensive analysis of PAD risk factor. Methods: Data from National Health and Nutrition Examination Survey (NHANES 1999-2004) were analyzed, including demographic, clinical, and dietary information. Nutrient intake was assessed through 24-h dietary recalls using computer-assisted dietary interview system (CADI) and automated multiple pass method (AMPM) methods. PAD was defined as an ankle-brachial index (ABI) < 0.9. Six ML models-extreme gradient boosting (XGBoost), random Forest (RF), naive bayes classifier (NB), support vector machine (SVM), logistic regression (LR), and decision tree (DT)-were trained on a 70/30 train-test split, with missing data imputed and sample imbalance addressed via synthetic minority oversampling technique (SMOTE). Model performance was evaluated using the area under the receiver operating characteristic curve (AUROC), accuracy, sensitivity, specificity, precision, recall, and F1 score. Shapley additive explanations (SHAP) analysis was used to identify key features. In addition, to further enhance the interpretability of the model, we applied SHAP analysis to identify the features that have a significant impact on PAD prediction. This approach allowed us to determine the contribution of different variables to the model's output, providing deeper insights into how each feature influences the prediction of PAD outcomes. Results: Of 31,126 participants, 4,520 met the inclusion criteria (mean age 61.2 +/- 13.5 years; 48.8% male), and 441 (9.7%) had ABI < 0.9. XGBoost outperformed other models, achieving an AUROC of 0.913 (95% CI, 0.891-0.936) and F1 score of 0.932. With SMOTE, its AUROC improved to 0.926 (95% CI, 0.889-0.936) and F1 score to 0.937. SHAP analysis identified vitamin C, saturated fatty acids, selenium, phosphorus, and protein intake as key predictors of PAD. Conclusion: This is the first study to apply ML algorithms to examine nutrient intake and PAD in a general population. Vitamin C and phosphorus showed negative correlations with PAD, while saturated fatty acids, protein, and selenium exhibited positive associations.

Background: Clinically useful predictors for risk stratification of long-term survival may assist in selecting patients for endovascular abdominal aortic aneurysm (EVAR) procedures. This study aimed to analyze the prognostic significance of peroperative novel systemic inflammatory markers (SIMs), including the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), hemoglobin-to-red cell distribution width ratio (HRR), systemic immune-inflammatory index (SIII), and systemic inflammatory response index (SIRI), for long-term mortality in EVAR. Methods: A retrospective analysis was performed on 147 consecutive patients who underwent their first EVAR procedure at the Department of Vascular Surgery, Beijing Hospital. The patients were divided into the mortality group (n = 37) and the survival group (n = 110). The receiver operating characteristic curves were used to ascertain the threshold value demonstrating the most robust connection with mortality. The Kaplan–Meier survival analysis was performed between each SIM and mortality. The relationship between SIMs and survival was investigated using restricted cubic splines and multivariate Cox regression analysis. Results: The study included 147 patients, with an average follow-up duration of 34.28 ± 22.95 months. Deceased patients showed significantly higher NLR (p < 0.001) and reduced HRR (p < 0.001). The Kaplan–Meier estimates of mortality were considerably greater in the higher-NLR group (NLR >2.77) and lower-HRR group (HRR <10.64). The hazard ratio (HR) of 0.833 (95% confidence interval (95% CI): 0.71–0.97, p < 0.021) was determined to be statistically significant in predicting death in the multivariable analysis. Conclusions: Preoperative higher-NLR and lower-HRR have been associated with a lower long-term survival rate in abdominal aortic aneurysm (AAA) patients undergoing elective EVAR. Multivariate Cox regression showed that decreased preoperative HRR is an independent risk factor that increases mortality risk following EVAR. SIMs, such as the NLR and HRR, could be used in future clinical risk prediction methodologies for AAA patients undergoing EVAR. However, additional prospective cohort studies are needed to identify these findings.

ObjectiveThis study aimed to conduct a meta-analysis evaluating the optimal timing for endovascular repair of acute versus subacute uncomplicated Type B Aortic Dissection.MethodPubMed, EMBASE, web of science and Cochrane Library was interrogated to identify Electronic bibliographic studies updated to January 2023 to collect studies compared the clinical outcomes of endovascular repair for Acute Versus Subacute Uncomplicated Type B Aortic Dissection. Data were aggregated as pooled odds ratios (OR) using the fixed or random effects models according to the significance of heterogeneity, Pooled odds ratios (OR) were calculated by RevMan 5.3 and applied with fixed or random-effect models.ResultA comprehensive literature search found 322 citations published and finally among them 6 studies containing 3,769 patients (acute group 2,642, subacute group 1,127) were included in review. There is an increased risk of 30-day complications (OR = 1.51,95%CI,1.26–1.81) 30-day mortality (OR = 2.39,95%CI, 1.55–3.67) and 1-year mortality (OR = 1.71,95%CI,1.27–2.30) for an acute uTBAD group compared to subacute ones. Similarly, reintervention was more likely in the acute group than in the subacute group (OR = 1.42,95%CI,1.05–1.91). However, no significant differences were found in long-term mortality.ConclusionThis meta-analysis confirmed that there was no significant difference in the long-term prognosis between the acute and subacute phases in the timing of surgery. However, considering the high incidence of complications, high re-intervention rate and one-year mortality probably caused by high intima fragility in the acute phase, endovascular repair at subacute phase appears to favorably compare with acute strategy. But future studies with adequate patient numbers and longer-term follow-up are necessary to further verify the study conclusion.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021247609, identifier PROSPERO CRD42021247609.

BACKGROUND:Endothelial cells are crucial in maintaining the homeostasis of the blood-brain barrier. Girders of actin filament (Girdin) and phosphor (p)-Girdin are essential for the engulfment of human brain microvascular endothelial cells (HBMECs) into platelets (PLTs), but the potential mechanism remains unclear and requires further study.METHODS:Following PLT and cytochalasin D treatment, Hoechst 33,342 detected apoptosis. The transfection efficiency of the short hairpin RNA targeting Girdin (sh-Girdin) or overexpressing Girdin (OE-Girdin) was determined using western blotting. Sh-Girdin, OE-Girdin, mutated Girdin (m-Girdin), and microfilament binding region deleted Girdin (Del-Girdin) were transfected into HBMECs under PLT conditions. Subsequently, the engulfment of HBMECs by PLTs was detected by flow cytometry and transmission electron microscopy. Girdin and phosphorylated (p)-Girdin levels were quantified by western blot. The positive expression of Girdin was measured by immunohistochemistry (IHC). The localization of PLT, Girdin, and p-Girdin and the engulfment of HBMECs in PLTs were analyzed by confocal microscopy.RESULT:Cytochalasin D overturned the inhibitory effect of PLT on cell apoptosis. OE-Girdin enhanced the fluorescent intensity of PLT-labelling and the engulfment of HBMECs by PLTs, while sh-Girdin, m-Girdin, and Del-Girdin ran reversely. OE-Girdin elevated the Girdin and p-Girdin levels, while sh-Girdin and Del-Girdin were the opposite, but m-Girdin did not affect the p-Girdin and Girdin levels.CONCLUSION:Girdin and p-Girdin were co-located with PLTs in HBMECs. The over-expression of Girdin was identified as being associated with the increasing engulfment of PTLs. Girdin may be an effective target to alleviate endothelial cell apoptosis.

Objective: Hyperlipidemia is a risk factor for cardiac damage that can lead to many cardiovascular diseases. A recent study reported the cardioprotective effects of luteolin in vitro and in vivo. In this study, we aimed to investigate the possible protective effects of luteolin against hyperlipidemia-induced cardiac damage in Sprague-Dawley (SD) rats.Methods: Six-week-old male SD rats were randomly divided into five groups: a normal diet (ND) group; a high-fat diet (HFD) group; and three high-fat diet mixed with luteolin (HFD + LUT) groups, where in a luteolin dosage 50, 100, or 200 mg/kg/day was administered. All groups were fed their respective diets for 12 weeks.Results: Left ventricular ejection fraction and fractional shortening (parameters of cardiac func-tion) were lower in the HFD + LUT (100 mg/kg/day) group than in the HFD group. Metabolic parameters were lower in the HFD + LUT (100 mg/kg/day) group than in the HFD group. Collagen I, collagen III, and TGF-& beta; expression levels were lower in the cardiac tissues of the HFD + LUT (100 mg/kg/day) group, compared to those of the HFD group. Expression of the profi-brotic genes MMP2 and MMP9 was suppressed in the cardiac tissues of the HFD + LUT (100 mg/ kg/day) group, compared to those of the HFD group. Furthermore, CD36 and lectin-like oxidized low-density lipoprotein receptor-1 protein levels were lower in the cardiac tissues of the HFD + LUT (100 mg/kg/day) group, compared to those of the HFD group. Conclusion: These findings would provide new insights into the role of luteolin in hyperlipidemia-induced cardiac damage and contribute to the development of novel therapeutic interventions to treat cardiovascular disease progression.

Background: Although hundreds of studies have been conducted, our understanding of the pathogenesis, indications for surgical intervention, and disease markers of Takayasu arteritis (TAK) are still limited. Collection of biological specimens, clinical data and imaging data will facilitate translational research and clinical studies. In this study, we aim to introduce the design and protocol for the Beijing Hospital Takayasu Arteritis (BeTA) Biobank. Methods: Based in the Department of Vascular Surgery of Beijing Hospital and Beijing Hospital Clinical Biological Sample Management Center, the BeTA Biobank is composed of clinical data and sample data from patients with TAK requiring surgical treatment. All clinical data of participants are collected, including demographic characteristics, laboratory tests, imaging results, operation information, perioperative complications, follow-up data, etc. Both blood samples including plasma, serum and cells, and vascular tissues or perivascular adipose tissue are collected and stored. These samples will promote the establishment of a multiomic database for TAK and help to identify disease markers and to explore potential targets for specific future drugs for TAK.

Previous studies have comprehensively evaluated predictive models related to peripherally inserted central catheter-related venous thrombosis (PICC-RVT) , but have not further elaborated on its application and effect in the tumor patient population. This article reviews the PICC-RVT evaluation tools that are clinically mature and applicable to cancer patients, analyzes the presentation forms, statistical methods, and limitations of different models, in order to provide suggestions and references for model selection by clinical healthcare professionals and the construction of future PICC-RVT prediction models for cancer patients.

目的 探讨大隐静脉主干射频消融术(RFA)+小腿段曲张静脉泡沫硬化剂注射治疗老年原发性大隐静脉曲张患者的疗效.方法 选取2017年10月至2020年3月北京中医药大学附属护国寺中医医院收治的260例老年原发性大隐静脉曲张患者为研究对象,采用随机数字表法分为观察组和对照组,每组130例.对照组患者采用高位结扎剥脱术治疗,观察组患者采用大隐静脉主干RFA+小腿段曲张静脉泡沫硬化剂注射治疗.比较两组术后3个月临床疗效,手术时间、住院时间、术中出血量、术前及术后12 h心率、术前及术后12 h平均动脉压(MAP)、术后7 d视觉模拟评分法(VAS)评分,术前及术后3个月血液流变学指标(血浆黏度、纤维蛋白原、低切全血黏度)、炎性指标〔基质金属蛋白酶13(MMP-13)、肿瘤坏死因子α(TNF-α)、IL-6、IL-17〕,并发症发生率,随访12个月记录患者复发情况.结果 观察组治疗总有效率为95.38％(124/130),高于对照组的84.62％(110/130)(χ2=8.376,P=0.004).观察组手术时间、住院时间短于对照组,术中出血量少于对照组,术后7 d VAS评分低于对照组(P<0.05).术后3个月两组血浆黏度、纤维蛋白原、低切全血黏度、MMP-13、TNF-α、IL-6、IL-17分别低于本组术前,且观察组血浆黏度、纤维蛋白原、低切全血黏度、MMP-13、TNF-α、IL-6、IL-17低于对照组(P<0.05).观察组并发症总发生率为8.46％(11/130),低于对照组的22.31％(29/130)(χ2=9.573,P=0.002).随访12个月,观察组复发率为2.31％(3/130),与对照组的4.62％(6/130)比较,差异无统计学意义(χ2=0.460,P=0.497).结论 大隐静脉主干RFA+小腿段曲张静脉泡沫硬化剂注射可提高老年原发性大隐静脉曲张患者治疗效果,明显缩短手术时间、住院时间,减少术中出血量,减轻疼痛程度,并能改善血液流变学指标及炎性指标,降低并发症发生率.

构建长期稳定的利益联结机制,是实现农业产业化的必然趋势,也是促进农民增收的重要途径.本文以湖南省家庭农场为研究对象,从制度效益、治理水平和成长能力等三个维度构建利益联结机制有效性的评价指标体系,运用熵权TOPSIS模型和障碍因子诊断模型,分析比较不同模式下家庭农场与农业企业利益联结机制的有效性,探讨其主要制约因素.结果表明,家庭农场与农业企业利益联结机制组织模式主要分为股份式、合同式和买断式三种,其有效性综合得分由大到小依次为股份式、合同式和买断式.从准则层权重来看,治理水平与制度效益权重较大,成长能力权重一般;从评价指标体系的三个维度来看,虽然股份式得分高于合同式和买断式,但三种模式得分普遍较低,仍有很大的提升空间.营业收入增长比例、违约成本、风险资金、带动农户数量、二次收入占比和担保金额是影响合同式和股份式的机制有效性的主要因素.鉴于此,建议有关部门在加强家庭农场与农业企业利益联结机制时应重点推动股份式利益联结模式的发展,提高家庭农场内生成长能力,创新风险分担和利益分配机制,增强合作主体的盈利能力.

Maintaining the health of the endothelium is of critical importance to prevention against cell aging. The current study was performed to clarify the role of sirtuin1 (SIRT1) in platelet phagocytosis in cell aging and identified its downstream molecular mechanism. Platelet phagocytosis by human endometrial microvascular endothelial cells (HEMECs) was characterized by transmission electron and fluorescence microscopy. Functional experiments were conducted to examine platelet phagocytosis and cell aging using the overexpression or knockdown plasmids of SIRT1 and G alpha-interacting, vesicle-associated protein (GIRDIN) as well as Akt inhibitor and activator. It was found that SIRT1 facilitated platelet phagocytosis by HEMECs, contributing to inhibition of cell aging. Akt activation facilitated platelet phagocytosis and repressed cell aging. GIRDIN overexpression accelerated platelet phagocytosis by HEMECs, leading to a delay in cell aging. GIRDIN phosphorylation at Ser1417 was induced by Akt activation, while activation of Akt was induced by SIRT1-mediated deacetylation, consequently augmenting platelet phagocytosis and delaying cell aging. Taken together, SIRT1 delayed aging of HEMECs by deacetylating Akt, phosphorylating GIRDIN, and inducing platelet phagocytosis. The study highlights a possible target for the prevention of HEMEC aging.

Objective:To evaluate the effect of remote endarterectomy in the treatment of complex lower extremity ischemia.Methods:Twenty-one limb ischemic patients underwent remote endarterectomy in Beijing Hospital from Sep 2016 to Feb 2020. Clinical data including general condition, the lesion of lower artery before operation and follow up outcomes were collected. Then the patency rate and limb salvage rate were calculated.Results:The technique success rate was 71.4% (15/21). The 3, 6, 12 month patency rate were 93.3%, 85.6% and 74.1%, respectively. The 1-year limb salvage rate was 93.3% (14/15). In the 6 patients converted to artificial vessel bypass, the 3,6,12 months patency rates were 76.7%, 66.7% and 46.8%, respectively. The 1-year limb salvage rate was 66.7%.Conclusions:Remote endarterectomy of the lower extremity artery is an alternative option in the treatment of complex ischemic lesions of the lower extremity artery, other than artificial vessel bypass.

Abstract Maintaining the health of endothelium is of critical importance to the prevention against cell aging. The current study was performed to clarify the role of SIRT1 on platelet phagocytosis in cell aging and identified its downstream molecular mechanism. Platelet phagocytosis by human endometrial microvascular endothelial cells (HEMECs) was characterized by transmission electron and fluorescence microscopy. Functional experiments were conducted to examine the platelet phagocytosis and cell aging using the overexpression or knockdown plasmids of SIRT1 and GIRDIN as well as Akt inhibitor and activator. It was found that SIRT1 facilitated platelet phagocytosis by HEMECs, contributing to inhibition of cell aging. Akt activation facilitated platelet phagocytosis and repressed cell aging. GIRDIN overexpression accelerated platelet phagocytosis by HEMECs, leading to delaying of cell aging. GIRDIN phosphorylation at Ser1417 was induced by Akt activation, while activation of Akt was induced by SIRT1-mediated deacetylation, consequently augmenting platelet phagocytosis and delaying cell aging. Taken together, SIRT1 delayed aging of HEMECs by deacetylating Akt, phosphorylating GIRDIN, and inducing platelet phagocytosis. The study highlights a possible target for the prevention of HEMEC aging.

Objective:To evaluate the efficacy and safety of heparin-bonded graft revascularization in patients with critical limb ischemia (CLI) after failed endovascular intervention.Methods:The clinical data of patients who underwent lower extremity bypass after failed endovascular intervention for CLIs from Oct. 2017 to Apr. 2019 were retrospectively analyzed. Individualized surgical therapy including stent removal, endarterectomy, graft or graft combined with autogenous vein bypass and other surgical methods to gain arterial revascularization of the lower extremity was designed based on the patients' symptoms and clinical features. Clinical characteristics, individual surgical therapeutic information, perioperative complications, symptom relief, ulcer wound healing, patency rate and limb salvage rate were analyzed.Results:A total of 27 patients were enrolled in this study. Resting pain of 16 cases relieved effectively. Among 11 cases of ulcer and tissue necrosis, 9 cases healed completely and 2 cases of ulcer reduced obviously half a year after operation. Postoperative complications occurred in 6 cases, with no perioperative death occurred. All patients were followed up for (13.0±8.9) months (range: 2-35 months). Kaplan-Meier estimated patency and limb salvage rate. Overall primary patency at 6-, 12- and 24-month was 83.3%, 73.7% and 49.1%, respectively; secondary patency was 91.8%, 82.1% and 70.8%, respectively; limb salvage was 91.8%, 86.9% and 76.6%, respectively. In the subgroup of femoropopliteal bypass, the 1- and 2-year primary patency rate was 86.7% and 49.5%, respectively; secondary patency was 93.3% and 81.7%, respectively; limb salvage was 93.3% and 81.7%, respectively. In the subgroup of femorocrural bypass, the 1- and,2-year primary patency rate was 45.0% and 45.0%, respectively; secondary patency was 58.3% and 58.3%, respectively; limb salvage was 58.3% and 58.3%, respectively.Conclusion:In the era of endovascular intervention, traditional arterial bypass can still provide a safe and effective alternative treatment for complex lesions of lower extremity arteries with failed endovascular intervention. It can efectively relive symptoms and improve limb salvage rate.

Objective To evaluate the efficacy of excimer laser atherectomy (ELA) combined with drug-coated balloon (DCB) in the treatment of lower limb arteriosclerosis obliterans.Methods From Sep 2016 to Dec 2018 42 patients (42 limbs) underwent ELA combined with DCB therapy.Results There were 27 males and 15 females.The mean age was (65.7 ±9.7) years.The average lesion length was (14.3 ±7.5) cm.The technical success rate was 100％ and the remission rate of clinical symptoms was 92.9％.Ankle-brachial index was significantly improved from (0.45 ± 0.15) pre-operation to (0.85 ± 0.13) postoperation (P =0.001).The mean follow-up was 10.9 months.At 3,6,and 12 months follow-up,the primary patency rate was 95.2％,92.8％ and 84.9％.The 1-year limb salvage rate was 95.2％.Conclusion ELA combined with DCB has a good 1-year results as our data reveal.

目的:探讨影响大动脉炎(TA)患者行开放手术治疗发生围手术期并发症的相关危险因素.方法:回顾性分析2003年1月-2018年12月136例行开放血管重建治疗的TA患者资料,采用单因素及多因素Logistic统计学方法分析影响开放手术围手术期并发症的相关危险因素.结果:136例患者共行开放手术141例次,涉及病变257处.围手术期并发症共发生36例次(25.5％),其中5例患者(3.7％)死亡.单因素分析结果显示,脑梗死病史、术前CRP升高、颈动脉受累、围手术期输血、血管受累数量、动脉阻断时间及术中出血量与围手术期并发症的发生有关(均P＜0.05);多因素Logistic分析显示,脑梗死病史(OR=3.141,95％ CI=1.062～9.288,P=0.039)、血管受累数量(OR=1.280,95％CI=1.016～1.612,P=0.036)和术中动脉阻断时间(OR=1.045,95％CI=1.007～1.084,P=0.019)是围手术期并发症的独立危险因素.结论:术前脑梗死病史,血管受累数量多和术中动脉阻断时间长会增加TA患者开放手术围手术期并发症的风险.

Endothelial cells are critical in angiogenesis and maintain the homeostasis of the blood‑brain barrier (BBB). Platelets (PLTs) are essential in vascular biology, including angiogenesis. The present study aimed to investigate the effect of PLTs on the aging of endothelial cells. Human brain microvascular endothelial cells (HBMECs) and human astrocytes were co‑cultured to mimic the BBB. Transmission electron microscopy was used to observe the engulfment of PLTs. Confocal microscopy was used to observe the co‑localization of PLTs, girders of actin filament (girdin) and phosphorylated (p‑)girdin. Senescence‑associated β‑galactosidase (β‑gal) staining, 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide and flow cytometry were performed to examine the cell senescence, viability and apoptosis, respectively. Transwell assays were performed to examine cell invasion and migration. Western blot analysis was performed to detect the expression of girdin, AKT and p‑AKT. PLTs delayed senescence, and promoted the viability and resistance to apoptosis of the HBMECs. Cell invasion and migration were enhanced by PLTs. In addition, girdin and p‑girdin were essential to the engulfment of HBMECs to PLTs. Mechanically, the inhibition of AKT signals reversed the effect of PLTs on HBMECs by increasing the activity of β‑gal, decreasing the cell viability, and inhibiting the invasion and migration of the HBMECs. The engulfment of PLTs assisted in delaying the aging of endothelial cells via girdin and p‑girdin, in which the AKT signal was involved. The present study indicated a potential strategy for delaying endothelial cell aging in the treatment of central nervous system diseases.

Long noncoding RNAs (lncRNAs) are commonly associated with various biological functions, in which the function of lncRNA maternally expressed gene 3 (MEG3) has been identified in various cancers. Strikingly, an association between MEG3 with microRNAs (miRNAs), mRNAs, and proteins has been reported. This study investigates the role of MEG3 in vascular endothelial cell (VEC) senescence. Expression of Girdin and miR-128 was monitored in the blood vessel samples of young and old mice/healthy volunteers, along with the measurement of human umbilical vein endothelial cells (HUVECs). The relationship between MEG3/Girdin and miR-128 was determined and verified. Loss- and gain-of-function approaches were applied to analyze the regulatory effects of MEG3 on platelet phagocytosis and lipoprotein oxidation of HUVEC membrane. In addition, the effect of MEG3 on HUVEC senescence was evaluated by detection of the reactive oxygen species, telomerase activity, and telomere length. To further analyze the MEG3-mediated regulatory mechanism, miR-128 upregulation and inhibition were introduced into the HUVECs. Downregulated Girdin and upregulated miR-128 were found in the blood vessels of old individuals and old mice, as well as in senescent HUVECs. MEG3 downregulation was found to be capable of inhibiting Girdin but enhancing miR-128 expression. It was also indicated to inhibit platelet phagocytosis and reduce telomerase activity and telomere length, while enhancing lipoprotein oxidation and reactive oxygen species production, which ultimately contributed in preventing and protecting HUEVCs from senescence. These findings provide evidence supporting that MEG3 leads to miR-128 downregulation and Girdin upregulation, which promotes platelet phagocytosis, thus protecting VECs from senescence.

Objective To explore the clinical value of the contrast-enhanced ultrasound（CEUS）in the diagnosis of renal artery stenosis（RAS）.Methods A total of 20 patients（12 males and 8 females）including 40 renal arteries,who were suspected to have RAS were enrolled in Department of Renal,Beijing Hospital.All patients were