目的 了解广西35～74岁壮族人群睡眠情况及其影响因素,为改善广西壮族人群睡眠质量提供参考依据.方法 通过"广西少数民族自然人群慢性病前瞻性队列研究"基线调查数据,选择11457名35～ 74岁的广西壮族人群为研究对象,应用匹兹堡睡眠质量指数(Pittsburgh sleep quality index,PSQI)量表评价研究对象的睡眠情况,采用x2检验以及多因素log-binomial回归模型分析影响睡眠质量的因素.结果 共纳入研究对象11457例,平均年龄为(54.09±10.45)岁,PSQi总均分为(4.86±2.95)分,睡眠不良发生率为18.67％(2139/11457).多因素log-binomial回归分析结果显示,女性(PR=1.169,95％CI:1.059～ 1.291,P=0.002)、高龄(PR=1.214,95％CI:1.118～ 1.317,P＜0.001)、低教育水平(PR=1.110,95％CI:1.022～ 1.206,P=0.013)、单身(PR=1.142,95％CI:1.019～1.272,P=0.019)、有夜宵习惯(PR=1.459,95％CI:1.294～1.636,P＜0.001)以及没有午睡习惯(PR=1.616,95％CI:1.496～1.745,P＜0.001)是睡眠不良的影响因素.结论 广西35～74岁壮族中老年人群睡眠不良发生率较高.性别、年龄、教育水平、婚姻状况、夜宵习惯和午睡习惯为主要影响因素.需进一步加大力度开展睡眠健康教育活动,针对睡眠影响因素进行干预,积极改善中老年人群睡眠质量.

目的 了解广西壮族老年人高血压患病情况及其影响因素,为针对该人群开展高血压综合防治提供科学依据.方法 采用方便抽样的方法,在2017-2019年期间对广西壮族自治区内年龄为60～74岁的4 251名壮族人群开展问卷调查、体格检查和血生化指标检测,了解广西壮族老年人的高血压患病情况.采用多因素非条件logistic回归分析高血压患病的影响因素,采用优势分析法计算影响因素对高血压患病的贡献度.结果 广西壮族老年人的高血压患病率为51.96％.多因素非条件logistic分析结果显示,年龄增长(OR = 1.05,95％CI:1.03～1.84)、男性(OR = 1.39,95％CI:1.23～1.58)、腰围异常(OR = 1.61,95％CI:1.39～1.84)、体质指数异常(OR = 1.19,95％CI:1.04～1.36)、甘油三酯(triglyceride,TG)异常(OR = 1.51,95％CI:1.30～1.75)和总胆固醇(total cholesterol,TC)异常(OR = 1.15,95％CI:1.02～1.31)是高血压患病的危险因素(P＜0.05).优势分析结果显示,影响因素对高血压患病贡献大小的前三位分别为腰围异常(33.31％)、TG异常(24.18％)、年龄(18.12％).结论 在广西壮族老年群体中,超过一半的老年人患有高血压,高血压患病与年龄、性别、腰围、BMI、TG和TC有关.控制腰围和降低血脂是该群体高血压防治的重点干预措施.

Objective To explore the association between potentially functional polymorphisms of rs2536( T C) and rs1883965( G A) in mammalian target of rapamycin( mTOR) and the risk of hepatocellular carcinoma( HCC),and to provide a reference for prevention of HCC. Methods A hospital-based case-control study was carried out. Totally 1 048 HCC patients and 1 052 cancer-free patients from the First Affiliated Hospital of Guangxi Medical University and Guangxi Cancer Hospital during January 2007- April 2011 were investigated with a questionnaire survey. Polymorphisms were genotyped for rs2536 and rs1883965 in the cases and controls. The associations between polymorphisms of rs2536 and rs1883965 in mTOR and its interaction with environmental factors and the risk of HCC were analyzed with logistic regression model. Results No significant differences were found in the genotype distributions of rs2536 and rs1883965( P 0. 05) between the cases and controls. The frequency of the TT,TC,CC,and TC + CC genotypes of rs2536 were 81. 01%,17. 75%,1. 24%,and 18. 99% in the cases and 80. 80%,17. 87%,1. 33,and 19. 20% in the controls,respectively. The frequency of the GG,GA,AA,and GA + AA genotypes of rs1883965 were 84. 06%,15. 74%, 0. 19%,and 15. 94% in cases and 83. 84%,15. 21%,0. 95%,and 16. 16% in the controls,respectively. After stratified by age,sex,race,smoking status,alcohol drinking,and hepatitis B virus infection,no statistically significant associations were found. Conclusion No association between polymorphisms of rs2536( T C) and rs1883965( G A) in mTOR and the risk of HCC was found in Guangxi population in the study.

目的:分析社会经济因素和粮油因素对广西流行性乙型脑炎(乙脑)发病率的影响及其影响程度.方法:采用多元回归分析方法筛选相关因素.结果:将广西75个县作为整体,以 2006～2010年乙脑发病率作为应变量Y,以经济和粮油指标的16个因素为自变量进行多元逐步进入回归分析,先后入选的变量是第三产业收入(b3=0.001 1)、社会固定资产投资(b5=0.000 4)、农民人均纯收入(b6=0.016 4)、居民储蓄存款(b7=-0.001).结论:社会经济因素对乙脑发病率具有一定的影响,粮油产量对乙脑发病率没有影响.

Objective To investigate the effect of DNA methylation inhibitor 5-Aza-2’-deoxycytidine (5-Aza-CdR) on the level of promoter methylation in human hepatoma cell line SMMC-7721 cells. Methods Human hepatoma cell line SMMC-7721 cells were treated with different concentrations (0.5μmol/L, 5.0μmol/L, 50.0μmol/L) of 5-Aza-CdR for 72 hours, with the untreated human hepatoma cell line SMMC-7721 cells as control group. The application of pyrosequencing was used to detect the average level of promoter methylation. Results DAPK gene promoter was highly methylated in human hepatoma cell line SMMC-7721 cells, with the average methylation level as 76.71%. When the cells were treated with different concentrations(0.5μmol/L, 5.0μmol/L, 50.0μmol/L)of 5-Aza-CdR for 72 hours, the average methylation level of DAPK gene for each treatment group was 78.29%, 77.57%and 66.00%, respectively, and statistically significant difference was found among different treatment groups (F=39.71,P<0.01). The average methylation level of DAPK gene in the 50.0μmol/L treatment group was lowest, compared with other groups (P<0.01). Conclusions 5-Aza-CdR can reverse the status of DAPK gene promoter methylation and induce the mRNA expresseion of DAPK in SMMC-7721 cells.