The mitochondrial unfolded protein response (UPRmt) is a stress response pathway that regulates the expression of mitochondrial chaperones, proteases, and other proteins involved in protein folding and degradation, thereby ensuring proper mitochondrial function. In addition to this critical function, the UPRmt also plays a role in other cellular processes such as mitochondrial biogenesis, energy metabolism, and cellular signaling. Moreover, the UPRmt is strongly associated with various diseases. From 2004 to 2022, there has been a lot of interest in UPRmt. The present study aims to utilized bibliometric tools to assess the genesis, current areas of focus, and research trends pertaining to UPRmt, thereby highlighting avenues for future research. There were 442 papers discovered to be related to UPRmt, with the overall number of publications rising yearly. International Journal of Molecular Sciences was the most prominent journal in this field. 2421 authors from 1,402 institutions in 184 nations published studies on UPRmt. The United States was the most productive country (197 documents). The top three authors were Johan Auwerx, Cole M Haynes, and Dongryeol Ryu. The early focus of UPRmt is "protein." And then the UPRmt research shifted from Caenorhabditis elegans back to mammals, and its close link to aging and various diseases. The top emerging research hotspots are neurodegenerative diseases and metabolic diseases. These findings provide the trends and frontiers in the field of UPRmt, and valuable information for clinicians and scientists to identify new perspectives with potential collaborators and cooperative countries.

痛经是妇科常见的一种疾病,指妇女正值经期或行经前后,出现周期性小腹疼痛,或痛引腰骶,或出现胃肠道反应,甚至剧痛晕厥者,亦称"经行腹痛".本病已严重影响了当今大多数女性的生活、学习和工作,探寻行之有效的治疗方法迫在眉睫.目前西医主要采用解痉止痛药、避孕药、激素等药物治疗,以及神经切除术、子宫或病灶切除术等手术治疗,能取得一定的临床疗效,但会引起头痛、乳房疼痛、疲倦、激素依赖、肥胖、内分泌紊乱等副作用.中医治疗本病的历史悠久,具有疗效显著、副作用小、费用相对较低等优点.因此,笔者分析了近10年来的相关文献资料,并结合古医籍的相关论述,从病因、病机、治疗等方面进行提炼和总结,以期为本病的进一步研究提供参考.

心律失常是临床常见的心血管疾病或并发症.属于中医"心悸""怔忡"范畴.临床治疗往往十分棘手,中医药治疗心悸病疗效显著.张丽梅主任是中国中医科学院广安门医院著名心血管病专家,临床治疗心律失常经验独到.本文总结了张丽梅主任对该病的辨证论治经验.张丽梅教授认为心悸的病机为本虚标实,根本病机为气阴两虚.其中本虚为气血阴阳亏虚,标实为痰湿、痰瘀、痰火之实.中医治疗以益气养阴、温通血脉为原则.临床重视整体观念,以及安神定悸、疏肝理气、健脾化痰、行气活血、熄风复脉等法的运用,效果显著,值得推广.

目的 比较气色形态手诊与耳垂皱褶征对冠心病的诊断效果,明确气色形态手诊诊断冠心病的临床价值.方法 纳入326例受试者,分别采用气色形态手诊和耳垂皱褶征进行冠心病诊断,并与冠状动脉造影诊断结果进行比较.结果 与冠状动脉造影诊断结果比较,气色形态手诊的敏感度为80.2％,特异度为68.7％,误诊率为31.3％,漏诊率为19.8％,阳性预测值为63.3％,阴性预测值为83.8％,准确率为73.3％;耳垂皱褶征的敏感度为58.8％,特异度为53.8％,误诊率为46.2％,漏诊率为41.2％,阳性预测值为46.1％,阴性预测值为66.0％,准确率为55.8％.气色形态手诊的诊断效果优于耳垂皱褶征.结论 气色形态手诊诊断冠心病的效果较好,具有较大的临床应用价值.

To explore the research status, hotspots, and trends in research on nicotinic acetylcholine receptor (nAChR) channel. The Web of Science core collection database from 2000 to 2020 was used as the data source. The visual analysis software VOSviewer1.6.16 and Citespace5.7 R3 were used to visualize the studies of the nAChR channel. The national/institutional distribution, journal distribution, authors, and related research were discussed. A total of 5,794 articles were obtained. The USA and the Utah System of Higher Education were the most productive country and institution for nAChR channel research. Journal of Biological Chemistry was the most productive journal (212) and the most productive researcher was McIntosh, J. Michael. The first highly co-cited article was "Refined structure of the nicotinic acetylcholine receptor at 4A resolution." The most researched area was neurosciences neurology. The hot spots of nAChR channel research were "subunit and structure of nAChR," "activation/agonist of nAChR channel," and "Changes in nAChRs With Alzheimer's Disease." The top three research frontiers of nAChR channel research were "neuropathic pain," "neuroinflammation," and "α7 nACHR." The study provides a perspective to visualize and analyze hotspots and emerging trends in the nAChR channel.

The transient receptor potential (TRP) channels, nonselective ion channels, mediate the fluxes of various types of cations across the cell membrane such as Na+, K+, Mg2+, and Ca2 + . TRPA (Ankyrin), TRPC (Canonical), TRPM (Melastatin), TRPV (Vanilloid), TRPP (Polycystin), and TRPML (Mucolipin) are TRP major families members. These channels play essential roles in diverse physiologic processes, and participate in virtually every sensory modality. TRPs can be activated by chemicals, temperature, stretch/pressure, osmolarity, pH, and so on, and play a major role in the five primary senses, such as vision, taste, hearing, smell, and touch. In recent years, TRP channels are widely studied in the field of nervous, intestinal, renal, urogenital, respiratory, and cardiovascular systems in diverse therapeutic areas including pain and itch, headache, pulmonary function, oncology, neurology, visceral organs, and genetic diseases [1]. Bibliometric analysis has been widely used to calculate the productivity of countries, institutions, authors, and the frequency of keywords to explore research hotspots/frontiers in specific fields [2–4]. In the present study, we performed a bibliometric analysis to systematically evaluate the TRP channels studies from 2012 to 2021 by CiteSpace and VOSviewer to provide researchers with some direction regarding TRP channels research [5,6]. Data source and search

手诊法为独具特色的中医望诊诊断方法之一.目前手诊冠心病诊断率尚缺乏科研数据报道,本文依托国家重点研发计划课题,概述了以刘剑锋气色形态手诊法为代表的多家手诊方法对冠心病诊断的经验,归纳了望手部颜色、形态、掌纹、指纹、指甲等不同手诊法诊断心系疾病的重要特征,总结了手诊诊断冠心病及心系病症的优势,提出笔者应用手诊诊断之经验及对手诊科学研究之展望,冀为进一步气色形态手诊法诊断冠心病的临床研究理清思路.

目的 运用Citespace软件对肾素-血管紧张素系统与心房颤动(房颤)关系的相关研究规律以可视化知识图谱的形式进行展示,以揭示该领域的研究方向、研究热点和研究前沿.方法 以Web of Science核心合集数据库为数据来源,利用可视化分析软件Citespace5.4 R3对肾素血管紧张素系统与房颤关系相关研究进行可视化分析,探讨相关研究的文献分布、国家/机构分布、作者分布以及对共被引文献和关键词进行分析.结果 共纳入503篇SCI文章,年度发文量和引文量总体呈现上升趋势;美国、中国、意大利、德国和日本为该领域研究的主要国家,加拿大蒙特利尔大学是该领域发文量最多的机构;发文量最高的作者是台湾大学的LIN JL及TSAI CT.主要的研究热点为房颤合并心力衰竭、心肌梗死研究,血管紧张素转化酶抑制剂或血管紧张素受体阻滞剂治疗房颤等研究.氧化应激在房颤发生中的作用,肾素血管紧张素抑制剂对心力衰竭、慢性肾病合并房颤的治疗作用,房颤的上游治疗药物的研究是肾素血管紧张素系统与房颤关系相关研究的前沿领域.结论 本研究运用Citespace软件对肾素血管紧张素系统与房颤关系的相关研究规律以可视化知识图谱的形式进行展示,揭示该领域的研究方向、研究热点和研究前沿,为研究人员进一步的研究提供指引与参考.

目的:随着国家"互联网+"战略的实施,医疗信息化向云计算转型已成为未来发展趋势,大力发展中医住院医师规范化培训是新医改的重要举措.方法:结合住院医师规范化培训工作的思考,以中国中医科学院广安门医院实践为例,研究构建中医住院医师规范化培训移动管理与服务的新模式.结果:引进基于微信平台的移动管理系统,操作简便,充分发挥移动互联网的特性,使用户和管理者可利用碎片时间参与医学教育,解决了传统管理模式中的难题.结论:新方法仍有待完善与提高,但系统平台管理显示出了明显的效率和意义,保证了住院医师培训的全面性和效果,间接地保证了医疗服务质量.

卵巢早衰是妇科内分泌疾病中的疑难病症,发病率逐年上升,且患者发病年龄逐年下降,严重危害女性身心健康.近年来中医药因其疗效好、安全性高以及个体化的优势受到广泛关注,关于卵巢早衰的中医治疗方面的研究也有了诸多新进展.本文通过对近年来相关文献的分析总结,将中医学对卵巢早衰的认识及相关研究进展综述如下,以便于进一步深入中医药对本病的研究.

目的 系统评价泰脂安治疗高脂血症的疗效和安全性.方法 计算机检索Cochrane图书馆、PubMed、EMbase、中国知网(CNKI)、中国生物医学文献数据库(CBM)、维普中文期刊服务平台(VIP)、万方数据库等数据库,纳入相关随机对照试验(RCTs),并进行评价,采用RevMan5.3软件分析数据.检索时限从建库至2018年4月20日.结果 最终纳入6篇RCTs,共631例受试者.相比对照组,泰脂安试验组的血脂总疗效高于对照组[RR=1.13,95％CI(1.05,1.22),P=0.001].治疗后总胆固醇(TC)水平与对照组效果接近[WMD=-0.33,95％CI(-0.84,0.19),P=0.21],对于合并阴虚证的病人,治疗后TC水平低于对照组[WMD=0.03,95％CI(0.22,0.28),P=0.82].治疗后三酰甘油(TG)水平与对照组效果接近[WMD=-0.03,95％CI(-0.23,0.16),P=0.72].治疗后高密度脂蛋白胆固醇(HDL-C)水平与对照组效果接近[WMD=0.15,95％CI(-0.06,0.36),P=0.15],对于合并阴虚证的病人,泰脂安治疗后HDL-C水平高于对照组[WMD=0.12,95％CI(0.04,0.20),P=0.004].治疗后低密度脂蛋白胆固醇(LDL-C)水平与对照组效果接近[WMD=-0.12,95％CI(-0.44,0.20),P=0.47],对于合并阴虚证的病人,泰脂安治疗后LDL-C水平低于对照组[WMD=-0.57,95％CI(-0.79,-0.34),P<0.00001].各组不良反应发生率差异均无统计学意义[RR=0.15,95％CI(0.01,3.60),P=0.24].结论 现有证据表明泰脂安治疗高脂血症疗效较好,对TC、TG、HDL-C、LDL-C的改善有效,在高脂血症兼阴虚证的病人中,对TC、HDL-C、LDL-C改善较明显,不良反应发生率未见与其他药物有明显差异.

随着我国医疗体系的快速发展,对于医保支付方式的改革也在不断深化.通过采用点数法能够有效的强调相对价值,促进医保总额的控制更加的准确.本文通过针对医保点数付费法进行相关研究,明确医保点数付费法的意义和作用,并且进一步促进医保点数付费法的水平不断提升.

目的 系统评价步长脑心通胶囊治疗高脂血症的疗效和安全性.方法 计算机检索Cochrane图书馆、PubMed、EMbase、Medline、CNKI、CBM、VIP、万方数据库等数据库,纳入相关随机对照试验(RCT),并进行评价,采用RevMan5.3软件分析数据.结果纳入35篇RCT,共3855例受试者.相比常规治疗或单独使用西药治疗(对照组),步长脑心通胶囊治疗(试验组)的血脂总疗效高于对照组[RR=1.21,95％CI(1.15,1.28),P<0.00001],治疗后总胆固醇(TC)水平低于对照组[WMD=-0.77,95％CI(-0.91,-0.64),P<0.00001],治疗后三酰甘油(TG)水平低于对照组[WMD=-0.58,95％CI(-0.68,-0.48),P<0.00001],治疗后高密度脂蛋白(HDL-C)高于对照组[WMD=0.20,95％CI(0.13,0.27),P<0.00001],治疗后低密度脂蛋白胆固醇(LDL-C)水平低于对照组[WMD=-0.58,95％CI(-0.73,-0.43),P<0.00001],各组不良反应发生率差异无统计学意义[RR=0.85,95％CI(0.58,1.24),P=0.40].结论 步长脑心通胶囊治疗高脂血症疗效明显,对 TC、TG、HDL-C、LDL-C的改善均较有效,不良反应发生率未见与其他药物有明显差异.

目的:探讨桑椹花青素-3-葡糖苷诱导乳腺癌细胞凋亡的作用及分子机制.方法:采用有机溶剂浸提和高效液相色谱法制备获得桑椹花青素-3-葡糖苷的纯品,使用不同浓度(200、400、600、800、1000μM)的桑椹花青素-3-葡糖苷作用于乳腺癌MDA-MB-231细胞24～72 h,并设置空白对照组和阳性对照组(30μM顺铂),CCK-8法检测细胞增殖情况;流式细胞术分析细胞凋亡水平;Western blot检测Cleaved caspase-3、easpase-3、Bcl-2和Bax蛋白表达水平;DNA梯度电泳检测DNA断裂水平.结果:与空白对照组相比,桑椹花青素-3-葡糖苷浓度高于200 μM时,MDA-MB-231细胞增殖受到明显抑制,MDA-MB-231细胞凋亡水平明显升高,且细胞增殖和凋亡水平呈剂量依赖效应;桑椹花青素-3-葡糖苷浓度高于600μM时,Cleaved caspase-3/caspase-3蛋白表达比率明显升高;桑椹花青素-3-葡糖苷浓度高于400 μM时,Bcl-2/Bax蛋白表达比率明显下降;随着桑椹花青素-3-葡糖苷浓度的升高,DNA断裂水平逐渐升高.结论:桑椹花青素-3-葡糖苷能够诱导乳腺癌细胞凋亡,其机制可能与调节Bcl-2/Bax/Cleaved caspase-3信号通路有关.

Objective To observe the characteristic changes of dynamic electrocardiogram in patients who were di.agnosed as premature ventricular contractions with syndromes of stirring wind due to yin deficiency.Methods From November 2011 to August 2016,90 patients who were diagnosed as premature ventricular contractions with syndromes of stirring wind due to yin deficiency in Guang'anmen Hospital,China Academy of Chinese Medical Sciences ("our hospital" for short) were selected as the premature ventricular contraction group,and 30 healthy subjects in our hospital were selected as the control group.All subjects underwent 24 hours dynamic electrocardiogram.The average heart rate,slowest heart rate,heart rate variability (HRV) of the two groups and the number of premature ventricular contraction in morning and night of premature ventricular contraction group,the correlation between TCM syndrome classification and HRV index in the premature ventricular contraction group were observed and analyzed.Results The levels of the average heart rate and the slowest heart rate in premature ventricular contraction group were all higher than those in control group (P ＜ 0.05).The levels of standard deviation of NN intervals (SDNN),standard deviation of average 5 min NN intervals (SDANN),percentage of differences exceeding 50 ms between adjacent normal number of intervals (PNN50),square root of the mean squared differences of successive RR interval (RMSSD) and HRV trigonometric index in premature ventricular contraction group were lower than those in control group (P ＜ 0.05).The premature ventricular contraction frequency of premature ventricular contraction group in the night was more than that in the morning (P ＜ 0.05).The syndrome classification of traditional Chinese medicine in premature ventricular contraction group was positively correlated with age,which was negatively correlated with SDNN,SDANN,PNN50,RMSSD,HRV triangular index.Conclusion The premature ventricular contraction frequency of patients in premature ventricular contraction group in the night is more than that in the morning,and the HRV is lower than control group.It is speculated that the occurrence of premature ventricular con traction with syndrome of stirring wind due to yin deficiency is positively related to autonomic nerve dysfunction,mainly vagus function decline.

OBJECTIVE To evaluate the efficacy and safety of Naoxintong capsule combined with statins in treating hyperlipidemia. METHODS We searched the Cochrane library, PubMed, Embase, Medline, CNKI, CBM, VIP and Wanfang database for RCTs, and analized by Revman 5.3 software. RESULTS Fourteen RCTs, a total of 1 244 subjects were included. Compared with the statins alone, the total effect of lipid of Naoxintong capsule combined with statins was higher than that in control group [RR=1.20, 95％CI (1.10, 1.31), P＜0.000 1], the TC level after treatment of Naoxintong capsule combined with statins was lower than that in control group [WMD=-0.79, 95％CI (-1.06, -0.53), P＜0.000 01], the heterogeneity of subgroup of Naoxintong capsule combined with Atorvastatin Calcium was low (P=0.09, The TG level after treatment of combined group was lower than control group [WMD=-0.68, 95％CI (-0.92, -0.45), P＜0.000 01], the HDL-C level after treatment of combined group was higher than control group [WMD=0.28, 95％CI (0.18,0.37), P=0＜0.000 01], the LDL-C level after treatment of combined group was lower than that in control group [WMD=-0.53, 95％CI (-0.87, -0.18),P=0.003]. The incidence of adverse reactions in each group showed no significant difference [RR=2.25, 95％CI (0.71, 7.16),P=0.17]. CONCLUSION Naoxintong capsule combined with statins may more effectively improve hyperlipidemia, including TC, TG, HDL-C and LDL-C, the efficiency of combined with statins for TC is exactly and there is no significant difference of adverse reactions compared with other drugs, this article recommend it used in the treatment of hyperlipidemia in clinical and to add more high quality researches.

目的 系统评价松龄血脉康胶囊治疗高脂血症的疗效和安全性.方法 通过计算机检索中国知网、中国生物医学文献数据库、万方、维普等中文数据库,并检索Cochrane、PubMed、MedLine、EmBase等外文数据库,纳入相关随机对照试验(RCT)进行评价,采用RevMan5.3软件分析数据.结果 纳入17篇RCTs,共1 488例病人.相较于西药组,松龄血脉康胶囊联合阿托伐他汀钙治疗的血脂总疗效高于对照组[相对危险度(RR)=1.26,95％置信区间(95％ CI) 1.07～1.49,P=0.006)];相较于西药组,松龄血脉康胶囊血脂总疗效改善率更高[RR=1.36,95％ CI (1.12,1.65),P=0.002],能有效升高高密度脂蛋白胆固醇(HDL-C)水平[加权均方差(WMD) =0.13,95％ CI (0.06,0.21),P=0.000 3],松龄血脉康胶囊联合西药能有效降低总胆固醇(TC)水平[WMD=-0.75,95％ CI(-1.16,-0.34),P=0.000 3]、三酰甘油(TG)水平[WMD=-0.50,95％ CI(-0.83,-0.16),P=0.004]、低密度脂蛋白胆固醇(LDL-C)水平[WMD=-0.47,95％ CI(-0.86,-0.08),P=0.02];相较于西药组,应用松龄血脉康胶囊联合氟伐他汀钠胶囊、阿托伐他汀钙治疗后HDL-C水平高于对照组[WMD=0.41,95％ CI (0.01,0.81),P=0.04][WMD=0.40,95％ CI (0.34,0.46),P=0.000 001];相较于中成药组,松龄血脉康胶囊治疗后TC水平低于复方丹参片组[WMD=-0.92,95％ CI(-1.26,-0.58),P=0.000 01],治疗后HDL-C水平高于复方丹参片组[WMD=0.42,95％ CI (0.22,0.62),P=0.000 1].结论 松龄血脉康胶囊对TC、TG、LDL-C改善更有效,其余指标与其他中成药及西药近似,安全性指标与其他药物相比未见明显差异.

目的 系统评价麝香保心丸治疗高脂血症的疗效及安全性.方法 计算机检索Cochrane图书馆、PubMed、Embase、CNKI、CBM、VIP、万方数据库,纳入相关RCT,并进行评价,采用RevMan 5.3软件分析数据.结果 纳入8篇RCTs共715例.相比对照组,麝香保心丸的心绞痛疗效较高[RR=1.20,95％CI(1.08,1.33),P=0.0008].治疗后总胆固醇水平低于对照组[WMD=-0.56,95％CI(-0.88,-0.23),P＜0.00001].治疗后甘油三酯水平低于对照组[WMD=-0.26,95％CI(-0.37,-0.15),P＜0.00001].治疗后高密度脂蛋白水平与对照组相比差异无统计学意义[WMD =0.04,95％CI(-0.06,0.15],P=0.42].治疗后低密度脂蛋白水平低于对照组[WMD=-0.28,95％CI(-0.51,-0.04),P=0.02].各项研究均未报道不良反应案例.结论 本文认为麝香保心丸治疗心绞痛疗效明显.对TC、TG、LDL-C改善整体有效,与常规治疗相比效果显著,但联合他汀类药物与单独使用他汀类药物相比差异未见统计学意义,对HDL-C的疗效仍需更多文献证明.推荐临床应用于冠心病合并高脂血症的治疗,并建议补充更多高质量的相关研究.

目的:观察消瘤汤内服配合中药灌肠治疗对子宫肌瘤的临床疗效.方法:采用消瘤汤内服配合中药灌肠进行治疗.结果:消失20例,显效7例,有效6例,无效7例.总有效率为82.5％.结论:采用消瘤汤内服配合中药灌肠治疗子宫肌瘤具有较好的临床疗效,值得临床推广应用.

Objective To evaluate the efficacy and safety of combined treatment of Wenxin Keli and bisoprolol for arrhythmia induced by coronary heart disease (CHD).Methods Papers about the RCTs of combined treatment of Wenxin Keli and bisoprolol for arrhythmias induced by CHD were searched from PubMed,Cochrane Central Register of Controlled TriMs,EMBASE,VIP,WanFang database and CNKI.Bias risk of included studies was judged.Meta analysis was done with RevMan 5.3 to analyze the literature data.Results 7 RCTs selected from 339 related articles involving 683 patients.The results of meta-analysis were as follows:for the improvement of arrhythmia [RR 1.33,95％ CI (1.23,1.45),P ＜ 0.01] and palpitation [RR 1.26,95％ CI (1.17,1.36),P ＜0.01],the group of combined treatment showed better results than the control group;for the rate of adverse reactions [RR 0.41,95％ CI (0.22,0.77),P ＜ 0.01],the group of combined treatment showed a lower rate than that of the control group.Conclusion The results showed to a certain extent that combined treatment of Wenxin Keli and bisoprolol is more effective and can reduce the adverse reactions in treating CHD-induced arrhythmias.