To investigate the latent infection status of porcine reproductive and respiratory syndrome virus(PRRSV) in Hebei Province,1 565 samples of lung or lymphonodi pulmonales from 228 slaughterhouses were tested for highly pathogenic porcine reproductive and respiratory syndrome virus(HP-PRRSV) and classical porcine reproductive and respiratory syndrome virus(C-PRRSV) by RT-PCR,respectively.In result,the positive rate of PRRSV in the slaughterhouses was 41.58%.The positive rate of HP-PRRSV was 12.08%,and the yearly positive rates were 28.45%,40.74%,12.01% and 7.31% from 2006 to 2009,respectively.The mean of C-PRRSV-positive rates were 2.36%,and the yearly positive rate was 3.45%,3.70%,3.90% and 1.46% from 2006 to 2009,respectively.Co-infection of PRRSV with other pathogens was also detected,which was mainly double or triple infection,and the co-infection rates of PRRSV,HP-PRRSV,C-PRRSV with other pathogens were 59.73%,57.67%and 43.24%,respectively.HP-PRRSV and C-PRRSV were not detected from the same sample.In summary,the latent infection of PRRSV,especially HP-PRRSV,was wide spread in pigs in Hebei Province,which deserves more attention.

To investigate the infection status of highly pathogenic porcine reproductive and respiratory syndrome virus(HP-PRRSV) in pigs from slaughterhouses in Hebei Province,1565 samples of lung or pulmonary lymphonodes were collected from 228 slaughterhouses and examined by RT-PCR.The results showed that the average HP-PRRSV positive rates were 28.45%,40.74%,12.01% and 7.31% from 2006 through 2009,respectively.Co-infection of PRRSV with other pathogens was also detected,which were mainly multiple infection,and the co-infection rates of HP-PRRSV with PRV,PCV2 and CSFV was 61.66%.In summary,the latent infection of HP-PRRSV was widely spread in pigs,which deserves more attention.

为了解河北省规模猪场PRRSV的变异情况,采用RT-PCR方法对2004-2009年采集的16个典型发病场的病料样本进行PRRSV Nsp2基因扩增、克隆和测序,利用DNAstar软件分析所测序列.结果表明,从样本中扩增的16个Nsp2基因中,有8份均在第481位和533～561缺失30个氨基酸,有8份没有缺失.表明,危害河北省规模猪场的PRRSV已经发生了较大的变异,其中2004-2005年的属低致病性PRRSV毒株,2006-2007年的属高致病性PRRSV毒株,而2008-2009年这两种毒株在规模猪场中均存在.

The ball controller will change his “Decision Model in the course of making a decision according to different areas in the field and different situations in a game. The experts' process in choosing “Decision Model while making a decision has obviously different “field characteristics and “competition situation characteristics due to his different places and oppressed situations. In 35m area round his penalty box the experts' “Decision Model bears both “field characteristics and “situation-characteristics. But in the middle field and in the 35m.area round opponent penalty box the experts' “ Decision Model only has clear “field characteristics and no obvious “competition situation characteristics is seen. The results demonstrate a similar “Decision Model between elite professional players , youth players and the experts. Compared with the experts, the juniors' “Decision Model is different.

对中国13个优秀女子足球队(包括国家队)及8个外国队的比赛进行了研究,临场技、战术统计指标33项.研究结果显示,我国优秀女子足球队运动员在比赛中全场40.9%～54.4%的时间在跑动.跑、走、停3者比例为1∶1.7∶0.28,运动员整场比赛活动距离平均为5 128 m.国内、外女子足球队在采用基本阵型上比较接近.阵地进攻是各队采用的主要打法,中路进攻突破运用次数最多,成功率最高的是边路进攻,进攻手段中传切配合效果最佳.快速对抗情况下传球技术运用质量较高.无论是快速、慢速、原地情况下接球,非对抗接球的次数都明显高于对抗中接球次数.快速情况下抢球的次数明显高于慢速、原地情况下抢球次数.中前场是运用传球、接球、抢球技术最多的场区.

对当前中国优秀女子足球运动员心理素质部分特征的现状进行了探讨与研究,其结果为高水平女子足球运动员的状态焦虑低于一般运动员,狭窄有效注意和广阔内在注意水平比其注意范围广度要好,在有效识别外界具体信息上有一定优势.凝聚力是影响队伍群体心理气氛最重要的因素.11项群体心理气氛维度指标可作为衡量一支球队心理环境乃至分析比赛成绩的1个客观标准.中国优秀女子足球运动员个性特征具有争强好胜、冒险敢为、少有顾忌、轻松兴奋、随遇而安的特点,拥有典型的项目特点,不同位置、不同训练年限组间存在差异,有个性特征.

控球队员随情况和位置的变化其战术思维决策活动的过程也在变化.即所在"场区"及其"对抗状态"不同,战术思维决策活动的主导因素是不同的.同一因素在此情况下成为主要因素,而在彼情况下又成为次要因素.专家组的战术思维决策活动过程在后场具有明显的"场区"特点和"对抗状态"特点,在中场和前场则表现出明显的"场区"特点而没有明显的"对抗状态"特点,训练水平高的职业队员及训练水平较高青年队员与专家组的战术思维决策活动变化有较高的相似趋势.相比之下少年队员与专家的战术思维决策活动过程有明显的差异.