Objective:To investigate the effects of serum medicated withWenyang Bushen Formula (温阳补肾方) on osteoclast apoptosis rate and RANK protein.Methods:Differentiation in vitro was made from the osteoclast precursor cells RAW264.7 to osteoclast,which was interfered respectively with low,medium and high dose ofWenyang Bushen Formula and normal saline serum.The flow cytometry was used to detect the apop-tosis rate of each group,and the Western Blot was used to detect the level of RANK protein in each group.Results:Compared with the blank control group,serum medicated withWenyang Bushen Formula could induce apoptosis of osteoclasts(P = 0.000),and reduce the expression of osteoclast RANK protein(P = 0.000).Conclusion:Serum medicated withWenyang Bushen Formula can induce apoptosis of osteoclasts,high dose of which is the best.

Objective: To explore the effects of serum containing Wenyang Bushen Formula on expression of bone resorption in osteoclasts.Methods: The preosteoclasts of RAW264.7 cell were induced to mature osteoclast by RANKL and M-CSF.Then cultured the mature osteoclast and the Wenyang Bushen Formula,low,medium and high dose group,and normal saline(NS) contained serum were respectively added.Vigour of serum TRACP were measured by biochem istry for cell culture fluid.The expression of Cts K and MMP-9 mRNA was measured by real time QPCR.Results: Compared with control group,Wenyang Bushen Formula group can significantly inhibited the TRACP activities(P0.01).The Expression of Cts K and MMP-9 in osteoclast of Wenyang Bushen group was significantly lower than NS group(P0.01).Conclusion: Wenyang Bushen Formula can effectively inhibit the expression of bone resorption in osteoclasts,and the effects of Wenyang Bushen Formula medium dose group is the best.

ABSTRACT:OBJECTIVE To investigate effect of Xinhuang tablets and its Chinese medicine components on high uric acid Mice Caused by hypoxanthine , and discusse its mechanism .METHODS The mice models of high uric acid caused by injecting hypoxanthine and the normal mice were adopted .After giving different doses of intragastric administration with extracts from Xinhuang tablets and its Chinese medicine components on the mice by gavage ,the uric and xanthine oxidase level of each group were determined .RESULTS After 1h of intraperitoneal injection of hypoxanthine ,positive control group and Xinhuang tablets and its Chinese medicine components dosage ( 23.75、47.5、95 mg? kg -1 ) could against high uric mice induced by hypoxanthine and reduced the activity of xanthine oxi -dase in hepar ( P<0.01 ) .CONCLUSION Xinhuang tablets and its Chinese medicine components could decrese the blood uric acid levels by inhibiting the activity of xanthine oxidase .

新癀片由肿节风、三七、人工牛黄、猪胆汁膏、肖梵天花和吲哚美辛等组成,具有清热解毒、活血化瘀、消肿止痛功效,主治热毒瘀血所致的咽喉肿痛,牙痛,痹痛,无名肿毒等症.新癀片中药组分是在新癀片的基础上去吲哚美辛而制成.近年来,新癀片在临床上也用于治疗痛风性关节炎[1-2],但未见新癀片对痛风性关节炎疗效的药效学报道.我们采用氧嗪酸钾引发小鼠出现高尿酸,观察新癀片及新癀片中药组分对小鼠血尿酸的影响.

<正>在西方国家的人口中,非酒精性脂肪性肝病患病率是20～30%,有2%～3%的非酒精性脂肪性肝炎(NASH)可发展到肝硬化和肝癌[1]。六味地黄丸是滋补肾阴的代表方,具有调节免疫、抗衰老、抗肿瘤、降血糖、降血脂、调节钙磷代谢、保肝等多种药理作用,对泌尿生殖系统、内分泌系统及心血管系统有明显影响[2]。我们采用高脂饲料建立NASH大鼠模型,观察六味地黄丸对NASH大鼠肝功能的影响。

<正>六味地黄丸有降血糖、降血压、降血脂、增强免疫、延缓衰老及防癌抗癌作用,对泌尿生殖系统、内分泌系统、心血管系统有明显的影响。我们用高脂饲料建立高脂大鼠模型,观察六味地黄丸混悬液对实验性高脂大鼠的血脂及主动脉的影