Objective:To investigate the application of different imaging methods of 99Tc m-pyrophosphate (PYP) in the diagnosis and pathological classification of cardiac amyloidosis (CA). Methods:A total of 31 patients (22 males, 9 females, age 21-81(57.2±13.4) years) with suspected CA who underwent 99Tc m-PYP dual-phase scintigraphy (early-phase: 1 h, delay-phase: 2-3 h) and SPECT/CT (1 h) between December 2018 and December 2019 in Peking Union Medical College Hospital were retrospectively included. Taking clinical diagnosis as the standard, the results of visual score (≥2, positive) and semi-quantitative values (heart to contralateral lung (H/CL)≥1.5, positive) of 99Tc m-PYP uptake in dual-phase scintigraphy and SPECT/CT imaging were analyzed. One-way analysis of variance and Bonferroni test were used to analyze the data. Results:Among 31 patients with suspected CA, 15 were clinically diagnosed as CA (5 patients with transthyretin-related CA (ATTR-CA) and 10 patients with light chain CA (AL-CA)) and 16 were diagnosed as non-CA. All 5 patients with ATTR-CA had positive dual-phase scintigraphy and SPECT/CT imaging results. Three out of 10 patients with AL-CA had positive early-phase scintigraphy whereas negative delay-phase scintigraphy and SPECT/CT imaging results. Sixteen patients who were clinically diagnosed as non-CA had negative dual-phase scintigraphy and SPECT/CT imaging results. The sensitivity (5/5), specificity (10/10), positive predictive value (5/5), negative predictive value (10/10) and accuracy (15/15) of delay-phase scintigraphy and SPECT/CT imaging were the same. Among 31 patients, 16 patients carried transthyretin-related (TTR) gene mutation, and 4 of them who clinically diagnosed as variant ATTR (ATTRv) had positive image findings while 12 of them who not clinically diagnosed as CA had negative image findings. There were significant differences in H/CL between ATTR-CA group and AL-CA group in early-phase (2.11±0.24 vs 1.31±0.07) and delay-phase (2.02±0.19 vs 1.30±0.05; F values: 75.41 and 87.15, Bonferroni test, both P<0.01). Conclusions:99Tc m-PYP delay-phase scintigraphy and SPECT/CT have high diagnostic efficiencies in ATTR-CA, helping to determine the pathological classification of CA; while early-phase scintigraphy has false positive results. Moreover, 99Tc m-PYP imaging is helpful to detect CA in patients with TTR gene mutation.

Background 99m Tc-PYP scintigraphy provides differential diagnosis of ATTR cardiomyopathy (ATTR-CM) from light chain cardiac amyloidosis and other myocardial disorders without biopsy. This study was aimed to assess the diagnostic feasibility and the operator reproducibility of 99m Tc-PYP quantitative SPECT. Method Thirty-seven consecutive patients who underwent a 99m Tc-PYP thorax planar scan followed by SPECT and CT scans to diagnose suspected ATTR-CM were enrolled. For the quantitative SPECT, phantom studies were initially performed to determine the image conversion factor (ICF) and partial volume correction (PVC) factor to recover 99m Tc-PYP activity concentration in the myocardium for calculating the standardized uptake value (SUV) (unit: g/ml). SUV max was compared among groups of ATTR-CM, AL cardiac amyloidosis, and other pathogens (others) and among categories of Perugini visual scores (grades 0–3). The intra- and inter-operator reproducibility of quantitative SPECT was verified, and the corresponded repeatability coefficient (RPC) was calculated. Results The ICF was 79,327 Bq/ml to convert count rate in pixel to 99m Tc activity concentration. PVC factor as a function of the measured activity concentration ratio in the myocardium and blood-pool was [ y = 1.424 × (1 − exp(− 0.759 × x )) + 0.104]. SUV max of ATTR-CM (7.50 ± 2.68) was significantly higher than those of AL (1.96 ± 0.35) and others (2.00 ± 0.74) (all p < 0.05). SUV max of grade 3 (8.95 ± 1.89) and grade 2 (4.71 ± 0.23) were also significantly higher than those of grade 1 (1.92 ± 0.31) and grade 0 (1.59 ± 0.39) (all p < 0.05). Correlation coefficient ( R 2 ) of SUV max reached 0.966 to 0.978 with only small systematic difference (intra = − 0.14; inter = − 0.23) between two repeated measurements. Intra- and inter-operator RPCs were 0.688 and 0.877. Conclusions 99m Tc-PYP quantitative SPECT integrated with adjustable PVC factors is feasible to quantitatively and objectively assess the burden of cardiac amyloidosis for diagnosis of ATTR-CM.

Introduction: L-isomer of 4-borono-2-F-18-fluoro-phenylalanine (L-[F-18]FBPA) was generally applied in clinic for boron neutron capture therapy (BNCT). With radiotracer validation, D-isomer of [F-18]FBPA (D-[F-18]FBPA) was found a higher tumor to normal brain tissue ratio (TBR) than its L-isomer on positron emission tomography (PET) in rat brain glioma. The present study was conducted as a first-in-human study to explore the biodistribution and radiation dosimetry of D-[F-18]FBPA in healthy human volunteers, comparedwith L-[F-18]FBPA. Methods: D-[F-18]FBPA or L-[F-18]FBPA was injected intravenously. Fivewhole-body PET scans were performed for each subject in the next 2 h. Organ time-activity curveswere drawn by measuring SUVmean in volumes of interest. Absorbed dose coefficient of target organs and effective dose (ED) were estimated on OLINDA/EXM. Results: Two healthy volunteers (both males) and three healthy volunteers (2 males, 1 female) were intravenously injected with D-[F-18]FBPA (5.5-7.2MBq/kg) and L-[F-18]FBPA (3.9-6.8 MBq/kg) respectively. Only limited accumulation of D-[F-18]FBPA was observed in healthy human brain, pancreas, liver, spleen and skeleton. The ED was calculated to be 0.026 mSv/MBq. Urinary bladderwall received the highest dose of 0.28 mGy/MBq, followed by kidneys (0.06 mGy/MBq), and all the other organs received less than 0.03 mGy/MBq. For L-[F-18]FBPA, higher uptake in brain, pancreas, liver, spleen and skeleton could be visualized, compared with D-[F-18]FBPA. The ED of L-[F-18]FBPA was 0.020 +/- 0.001 mSv/MBq. Urinary bladder wall and kidneys still received the highest dose among organs but with lower values than those of D-[F-18]FBPA. Conclusions: D-[F-18]FBPA had lower activity in normal brain, liver, spleen, pancreas and skeleton, compared with its L-isomer. D-[F-18]FBPA is safe from a radiological standpoint. Advances in knowledge and implications for patient care: D-[F-18]FBPA was safe from a radiological standpoint, and had lower activity in normal brain, liver, spleen, pancreas and skeleton than its L-isomer. This study ensures the safety and validity of D-[F-18]FBPA for further clinical trials in patients with cancer. (C) 2021 Elsevier Inc. All rights reserved.

A reliable copper-mediated nucleophilic radiosynthesis of the PET imaging probe [18F]FBPA was developed using novel aryldiboron precursors. The carrier-free [18F]FBPA with radiochemical purity >99% was prepared routinely via the two-step synthesis with an automatic module and can be used for clinical PET imaging of tumours.

Objective:To evaluate the diagnostic value of 99Tc m-pyrophosphate (PYP) in transthyretin cardiac amyloidosis. Methods:From December 2018 to July 2019, 17 patients (9 males, 8 females, age: (53.4±13.0) years) with suspected cardiac amyloidosis underwent 99Tc m-PYP imaging in Peking Union Medical College Hospital were prospectively included. Visual score and semi-quantitative values (heart to contralateral ratio, H/CL) of 99Tc m-PYP uptake were used to diagnose transthyretin amyloidosis (ATTR). Biopsies and genetic measurements were also developed to evaluate the diagnostic value of the imaging. Results:Five of the 17 patients were diagnosed as ATTR with a visual score of 2-3, H/CL≥1.5, and confirmed with the biopsy or gene test. Four patients were diagnosed as ATTR with positive genetic results but no cardiac symptoms, and their visual scores were between 0 and 1 with H/CL<1.5. Considering the young age of the patients, amyloid deposition might have not yet caused visceral damage. Visual score of other 8 patients with negative 99Tc m-PYP imaging were also between 0 and 1 with H/CL<1.5, 2 of 8 were confirmed with light chain amyloidosis (AL) by biopsy, 3 were clinically diagnosed as AL and 3 were ATTR excluded. The accuracy of 99Tc m-PYP imaging for diagnosing ATTR was 11/11. Conclusion:99Tc m-PYP imaging is helpful for non-invasive diagnosis of transthyretin cardiac amyloidosis.

本工作探讨了新型示踪剂99 Tcm-HYNIC-3PEG4-E[c(RGDfK)]2(99 Tcm-3PRGD2)用于胰腺癌的诊断价值及临床转化可行性.用免疫组化实验测定PA NC-1胰腺癌细胞以及肿瘤组织中的整合素αvβ3的表达.将PANC-1胰腺癌细胞种植于BALB/c裸鼠肩部,建立合格的动物模型.以联肼尼克酰胺(HYNIC)作为双功能连接剂,采用无亚锡一步法制备99 Tcm-3PRGD2.在0.5～6.0 h,每隔0.5 h行一次荷瘤鼠99 Tcm-3PRGD2全身平面显像,观察全身分布情况,于肿瘤及健侧勾画感兴趣区(ROI,region of interest),计算肿瘤与本底(T/NT)的比值.对6例胰腺肿瘤患者行99Tcm-3PRGD2单光子发射的计算机断层显像(SPECT),评估对胰腺癌的检出率.结果表明:PANC-1胰腺癌细胞以及肿瘤组织中高表达整合素αvβ3.99 Tcm-3PRGD2标记率大于99％.荷瘤鼠显像显示肿瘤对示踪剂摄取好,0.5 h时即可见肿瘤显影,1.5 h时T/NT达最大值,6.0 h时肿瘤仍可清晰显示.99 Tcm-3PRGD2 SPECT显像可检出6例患者的胰腺肿瘤原发灶和肝转移灶,检出率为100％.99 Tcm-3PRGD2是一种安全有效的示踪剂,特异性结合整合素αvβ3,99 Tcm-3PRGD2 SPECT对胰腺癌的临床诊断具有潜在的应用前景.

目的 探讨放射性核素99mTc-标记红细胞(99mTc-RBC)显像在消化道出血急重症患者中的临床价值以及影响因素.方法 选取本院2012年7月至2016年2月54例临床诊断为消化道出血患者,进行消化道出血放射性核素显像检查,其中49例接受了消化道内窥镜检查,33例接受了CT相关检查,12例接受了手术治疗.采用体外法标记红细胞(99mTc-RBC),行腹盆部前后位图像采集,至有阳性结果 ,如无阳性结果则延迟至24小时.结果 所收录患者均为急性起病,或慢性病程急性发作,因合并急性冠脉综合征、出血性休克、急性肠梗阻以及其他需要抢救的重症患者共31例;54例患者行核素消化道出血显像,检查阳性为44例,阳性率为81.5%,敏感性为97.7%,特异性为81.8%.33例接受CT相关检查,15例阳性,阳性率为45.4%.49例接受内窥镜检查,阳性38例,阳性率为77.6%.44例阳性患者中,提示胃及十二指肠阳性11例,提示小肠阳性25例,提示结肠阳性4例,全消化道多处阳性4例.其中26例经相应部位内镜检查和(或)手术证实,7例没有行相应部位内镜检查,11例仅提示存在消化道出血却无法准确定位,定位有效性为70.3%.结论 核素消化道出血显像方便、快捷,覆盖面积大,能够指导急性消化道出血的精准检测及治疗.患者病情允许下可暂停止血药以提高检出率.

肝癌是最常见恶性肿瘤之一,我国癌发病率和死亡率远高于西方发达国家.对于尚未出现远处转移的肝癌,手术切除是首选的方法之一.然而,肝癌患者往往同时伴有慢性肝病和肝功能受损,手术前精准判断残余肝脏功能对于避免患者术后肝功能失代偿非常重要.为此,研究团队在99mTc-GSA显像预测术后肝脏功能方面开展了系列基础和临床研究.本研究从图像采集方法学的角度,探讨如何优化99mTc-GSA肝功能显像的采集信息和参数,以更方便、精准地评估肝脏储备功能.

BACKGROUND:Available tools in liver surgery planning rely on the future remnant liver (FRL) volume. Inappropriate decision might be made since the same FRL volume might represent different liver functions depending on the severity of underlying liver damage. This study developed an alternative system to estimate FRL function and to predict the risk of postoperative liver failure.METHODS:Current study recruited 71 prehepatectomy patients and 71 healthy volunteers. A technetium-99-labelled asialoglycoproteins was given to participants and SPECT was used to capture the intensity of the signal, represented by uptake index (UI). The agreement between preoperative UI values, liver function tests, and Child scores were evaluated. Linear regression was used to evaluate the agreement between predicted UI for FRL and postoperative UI values. Area under the receiver operating characteristic (AUC) curve was used to evaluate the discriminative performance of UI in differentiating patient with high risk of liver failure.RESULTS:Preoperative UIs are highly correlated with Child score (P < 0.0001), especially to identify patients with ascites and elevated bilirubin. The predicted UIs were in close agreement with the actual postoperative UI values (r = 0.95 P < 0.001). The AUC analysis indicated that UI values had a high accuracy in predicting the risk of liver failure (AUC = 0.95, P < 0.0001). The best cut-off point was 0.9 and the corresponding sensitivity was 100 % and specificity was 92 %.CONCLUSIONS:The new methodology reliably estimates FRL function and predicts the risk of liver failure. It provides a visual aid for liver surgeon in surgery planning and risk assessment.

OBJECTIVES:Tumor-induced osteomalacia (TIO) is generally caused by small benign mesenchymal tumors producing fibroblast growth factor-23 (FGF-23). The only curative therapy of the disease is resection of the causative tumors. However, these tumors are extremely difficult to detect using conventional imaging modalities. This research was undertaken to evaluate efficacy of (68)Ga DOTATATE PET/CT in this clinical setting.METHODS:Images of (68)Ga DOTATATE PET/CT and clinical charts from 54 patients with clinically suspected TIO were retrospectively reviewed. The image findings were compared with the results of histopathological examinations and clinical follow-ups.RESULTS:(68)Ga DOTATATE PET/CT scans were positive in 44 patients, among which, 33 had surgery to remove the lesions. Postsurgical pathological examination confirmed causative tumors in 32 patients whose symptoms diminished promptly, and the serum phosphate levels became normal, which confirmed the diagnoses of TIO. Eleven patients with positive (68)Ga DOTATATE PET/CT did not have surgery. These 11 patients continued to have symptoms and hypophosphatemia but were not included in the final analysis because of lack of evidence to confirm or exclude TIO. Ten patients had negative (68)Ga DOTATATE PET/CT scans. All of these10 patients responded to conservative therapy and had normal serum phosphate levels in the follow-up, which excluded TIO. Therefore, the (68)Ga DOTATATE PET/CT imaging had a sensitivity of 100% (32/32) and a specificity of 90.9% (10/11). The overall accuracy of (68)Ga DOTATATE PET/CT scan in the detection of tumors responsible for osteomalacia is 97.7% (42/43).CONCLUSIONS:(68)Ga DOTATATE PET/CT scan is an accurate imaging modality in the detection of tumors causing TIO.

目的 探讨131I-BDI-1在荷人膀胱癌裸鼠模型中的体内生物分布和对肿瘤的靶向定位性能以及其生物安全性,并由此估算131I-BDI-1在人体内主要脏器的内照射吸收剂量和人体有效剂量.方法 通过氯胺-T法用131I溶液直接标记抗人膀胱癌单克隆抗体BDI-1,制备131I-BDI-1,荷人膀胱癌EJ细胞裸鼠尾静脉注射222kBq 131I-BDI-1后,于不同时刻处死动物,取感兴趣器官和组织,称重并测量放射性计数,计算131I-BDI-1在荷瘤裸鼠中的标准摄取值和靶/非靶组织比值.利用标准的MIRD数据表计算人体的％ID/g,通过MIRDOSE3.1软件估算131 I-BDI-1对人体各个器官的辐射吸收剂量及有效剂量.结果 生物分布数据示131I-BDI-1血液清除缓慢,肿瘤部位放射性明显持续滞留,除血液外,具有较高的靶/非靶组织比值.131I-BDI-1人体内照射的有效剂量为1.46×10-2 mGy/MBq.结论 131I-BDI-1对肿瘤有良好的靶向性,有望成为一种新型的针对人膀胱癌肿瘤的诊治药物,其辐射吸收剂量较低,作为肿瘤治疗药物是安全的.

Objective To evaluate the effectiveness and safety of higenamine (HG),a pharmaco logical stress agent,for the detection of myocardial ischemia using SPECT.Methods This study was an open,multi-center,randomized and positively controlled trial with crossover references.It consisted of 120patients clinically confirmed or suspected of myocardial ischemia.Each patient underwent a resting MPI and two separate stress MPI in a randomized crossover manner with intravenous administration of HG or adenosine (Ad) on different days.The severity and extent of myocardial ischemia were diagnosed on stress MPI.The degree of vascular stenosis in terms of percentage narrowing was measured by CAG (＞50％ was defined as coronary disease),thus defined as gold standard.The diagnostic efficacy of HG and Ad was compared.Vital signs,routine blood and urine tests,blood biochemical items and side effects were documented for evaluation of procedure safety.Two-sample t test,x2 or Fisher's exact test,and Kappa test were used.Results A total of 109 patients completed the trim and CAG.The diagnostic sensitivity,specificity,accuracy,positive predictive value,negative predictive value of HG MPI were 56.1％ (32/57),78.8％ (41/52),67.0％(73/109),74.4％ (32/43) and 62.1％ (41/66),respectively,which were not significantly different from those ofAd MPI (52.6％ (30/57),82.7％ (43/52),67.0％ (73/109),76.9％ (30/39) and 61.4％(43/70) ;x2 =0-0.2476,all P＞0.05).The sensitivity of HG vs Ad MPI in the diagnosis of single-,double-and triple-vessel ischemia was 29.6％ (8/27) vs 22.2％ (6/27),64.7％ (11/17) vs 64.7％ (11/17)and 100％ (13/13) vs 100％ (13/13),respectively.The concordance between HG and Ad for the detection of LAD,LCX and RCA ischemia was 95.41％ (104/109),97.25％ (106/109) and 97.25％ (106/109) (Kappa=0.8905,0.8420 and 0.8874).HG did not induce significant systolic blood pressure change during or after administration.Both HG and Ad could induce temporary decrease of diastolic blood pressure.Either HG or Ad induced significantly increased HR during administration and 5 min after administration.The clinical laboratory profile (hematology,serum chemistry,and urinalysis) was either normal or with no significant change.A total of 176 side effects (e.g,dyspnea,short breath,palpitation,dizziness,headache) were found related to HG (69.2％,83/120) and Ad (77.5％,93/120) administration (x2=2.1307,P＞0.05),which were mostly mild and transient.Conclusion HG is a safe and effective pharmacological stress test agent as compared to adenosine for the detection of CAD with SPECT perfusion imaging.

目的研究金属核素多功能模块自动化及手工合成68Ga-1, 4, 7, 10-四氮杂十二烷-N, N, N, N-四乙酸-D-苯丙氨酸1-酪氨酸3-苏氨酸-8-奥曲肽(68Ga-DOTATATE)的可行性, 并探讨其生物学分布及肿瘤显像特点。方法采用强阳离子交换(SCX)柱金属核素多功能模块自动化及手工方法合成68Ga-DOTATATE, 并对产品注射液进行质量控制。取ICR小鼠30只, 分为5组, 分别于注射68Ga-DOTATATE后10、30、60、120和240 min处死, 取各脏器、称质量并测定放射性计数, 计算放射性摄取值(%ID/g), 观察生物学分布。对荷AR42J大鼠胰腺外分泌腺肿瘤裸鼠注射68Ga-DOTATATE后行microPET显像, 观察肿瘤对68Ga-DOTATATE的摄取情况。结果金属核素多功能模块自动化与手工合成2种方法均能顺利合成68Ga-DOTATATE。产品均为无色澄明溶液, pH值为6.5±0.1, 放化纯均大于99%, 室温放置4 h仍大于99%。自动化合成需时约30 min, 放化产率为(51.8±3.2)%(时间衰减校正);手工合成需时约20 min, 标记率大于99%。14 d细菌培养及细菌内毒素检查结果均符合规定。ICR小鼠体内生物学分布结果显示, 肾脏为药物排泄器官, 肝、脾、胰腺和肾上腺摄取较高, 肌肉软组织摄取低;药物的血液清除快, 10 min为(4.41±0.81) %ID/g, 1 h时仅为(0.78±0.32) %ID/g。荷AR42J瘤裸鼠microPET显像示, 肿瘤组织对68Ga-DOTATATE明显高摄取, T/NT分别为2.01±0.29(10 min)、6.74±2.90(30 min)和4.46±2.05(60 min)。结论 68Ga-DOTATATE合成工艺简单、质控合格、动物实验结果良好, 是一种有潜在应用前景的新型生长抑素类特异性正电子显像剂。

Objective To evaluate the effectiveness and safety of higenamine （HG）, a pharmacological stress agent, for the detection of myocardial ischemia using SPECT. Methods This study was an open, multi-center, randomized and positively controlled trial with crossover references. It consisted of 120 patients clinically confirmed or suspected of myocardial ischemia. Each patient underwent a resting MPI and two separate stress MPI in a randomized crossover manner with intravenous administration of HG or adenosine （Ad） on different days. The severity and extent of myocardial ischemia were diagnosed on stress MPI. The degree of vascular stenosis in terms of percentage narrowing was measured by CAG （ 〉50% was defined as coronary disease）, thus defined as gold standard. The diagnostic efficacy of HG and Ad was compared. Vital signs, routine blood and urine tests, blood biochemical items and side effects were documented for evaluation of procedure safety. Two-sample t test, X2 or Fisher＇s exact test, and Kappa test were used. Results A total of 109 patients completed the trial and CAG. The diagnostic sensitivity, specificity, accuracy, positive predictive value, negative predictive value of HG MPI were 56.1% （32/57）, 78.8% （41/52）, 67.0% （73/109）, 74.4% （32/43） and 62.1% （41/66）, respectively, which were not significantly different from those of Ad MPI （52.6% （30/57）, 82.7% （43/52）, 67.0% （73/109）, 76.9% （30/39） and 61.4% （43/70） ;X2 =0-0.2476, all P〉0.05）. The sensitivity of HG vs Ad MPI in the diagnosis of single-, doub- le- and triple-vessel ischemia was 29.6% （8/27） vs 22.2% （6/27）, 64.7% （11/17） vs 64.7% （11/17） and 100% （13/13） vs 100% （ 13/13）, respectively. The concordance between HG and Ad for the detec- tion of LAD, LCX and RCA ischemia was 95.41% （ 104/109）, 97.25% （106/109） and 97.25% （ 106/ 109） （Kappa = 0.8905, 0.8420 and 0.8874）. HG did not induce significant systolic blood pressure change during or after administration. Both HG and Ad could induce temporary decrease of diastolic blood pressure. Either HG or Ad induced significantly increased HR during administration and 5 min after administration. The clinical laboratory profile （hematology, serum chemistry, and urinalysis） was either normal or with no significant change. A total of 176 side effects （e.g, dyspnea, short breath, palpitation, dizziness, headache） were found related to HG （69.2%, 83/120） and Ad （77.5%, 93/120） administration （X2 = 2. 1307, P〉0.05）, which were mostly mild and transient. Conclusion HG is a safe and effective pharma- cological stress test agent as compared to adenosine for the detection of CAD with SPECT perfusion imaging. Key words: Coronary disease ; Higenamine hydrochloride ; Adenosine ; Tomography, emission-computed, single-photon ; MIBI

目的 评价99mTc-HYNIC-TOC生长抑素受体显像诊断和鉴别诊断胰岛素瘤的临床价值.方法 回顾性分析近11年来65例胰岛素瘤患者的99mTc-HYNIC-TOC显像结果,并与胰腺灌注CT、B超或内镜超声结果比较.结果 65例患者均进行99mTc-HYNIC-TOC显像,阳性28例,检出率43.1％.部分患者进行胰腺灌注CT、B超或内镜超声检查,检出率分别为92.5％、25.6％和76.9％.结论 单独应用99mTc-HYNIC-TOC显像对胰岛素瘤的定性诊断和鉴别诊断临床价值有限,需联合应用多项分子影像诊断技术.

1464 Objectives Generator-produced 68Ga has attracted increasing interest for radiolabelling peptides used in PET applications. So it is necessary to develop an approach for the synthesis of 68Ga-labelled peptides automatically. Here we describe such an approach with PC-controlled process using a metal isotope multifunction module which was made in China. Methods Reaction parameters such as reaction temperature and timewere optimized for labeling DOTATATE. Reaction yields, radiochemical purity, breakthrough of 68Ge and final 68Ge content were determined. The automatic process was as below. A 68Ga generator was eluted with 0.05mol/l HCl through a peristaltic pump, of which the flow rate is 2ml/min. After 40 seconds the eluate was passed through a Strata X-C cartridge. The cartridge was washed with 2ml deionized water, blowed-dry with N2 flow and eluted with 0.6ml 98% acetone 0.02mol/l HCl. The eluate entered the reaction vessel in which 40μg DOTATATE was added. After 5ml deionized water was added, the reaction mixture was heated at 130°C for 2min and 100°C for 10min, then cooled by adding 4ml deionized water. The solution was transferred to a pre-conditioned Sep-Pack Light C18 cartridge, which was washed with 15ml deionized water. The C18 cartridge was eluted with 1ml 75% ethanol over a sterile filter into the final vial. Subsequently, the cartridge and sterile filter were washed with 7ml saline to yield a final ethanol content Results 68Ga-DOTATATE was successfully synthesized with the module in about 60%(time decay-corrected) radiochemical yield in about 20 min. The radiochemical purity was more than 98%. The SPE purification could avoid any potential 68Ge breakthrough, final activities of 68Ge were not detected. Moreover, it was demonstrated that NOTA-derivatized peptides can be labeled using the identical reaction conditions with high yields. Conclusions The system described allows automated and rapid preparation of 68Ga labeled peptides in good yield and radiochemical purity. It’s reliable for the routine preparations in clinical studies