培养大学生的创新能力是高等教育的重要使命,特别是高等医学教育更加需要注重培养学生的创新精神与创新能力.为提高细胞生物学实验课程的教学效果,确立了以下的综合教学改革模式:改进教学方法,强调自主学习;优化实验设计,培养创新思维;改革评价体系,鼓励创新探索;参与大创项目,进行创新实践.教学实践表明,这些措施提高了学生的学习积极性和主动性,培养了学生的创新意识和创新能力,取得了良好的教学效果.

信息时代,知识传播、获取途径发生了深刻变化,这就对高等教育提出了更高要求.作为传授知识的重要主体,高校教师的社会角色也必然发生深刻变化.笔者认为,高校教师应该强化现代教育理念,掌握现代教育技术,培养终身学习意识,重视教学科研互长,积极参加社会实践,以应对新时代的挑战.

Thladiantha dubia Bunge has extensively application in the folk.In recent years,lots of researches about Thladiantha dubia Bunge were carried out for its planting,identification,quality control,chemical composition,activity,and so on.This article mainly summarized the identification,components,extraction process,quality control and pharmacological of Thladiantha dubia Bunge,which would provide scientific data for the development and utilization of the Thladiantha dubia Bunge.

目的 研究槲皮素对过氧化氢(H2O2)诱导脐静脉内皮细胞(HUVECs)凋亡的影响.方法 体外培养脐静脉内皮细胞,H2O2作用于HUVECs诱导其凋亡,采用MTT法检测细胞活力,流式细胞术检测细胞凋亡率,Western-blot检测GRP78、XBP1及caspase-3蛋白表达.结果 H2O2呈剂量依赖性降低内皮细胞活力,并引起细胞凋亡.其中500 μmol/L H2O2作用于细胞后,与正常组相比,细胞活力明显受到抑制,且GRP78、XBP1、caspase-3等蛋白含量明显增加(P＜0.01);槲皮素预处理后,随着槲皮素浓度增加,细胞活力随之增加;与模型组相比,10 μmol/L槲皮素能够显著抑制细胞凋亡,GRP78、XBP1、caspase-3等表达降低(P＜0.01).结论 槲皮素能抑制H2O2诱导的内皮细胞凋亡,该作用可能与下调GRP78、XBP1、caspase-3等表达有关.

为适应现代医学模式的要求,培养既能治病救人,又能预防疾病、保护和促进人类健康的合格医学生,中医药院校预防医学课堂教学需改变传统陈旧的教学模式,调动学生学习的热情和积极性.情景模拟教学法可以活跃课堂气氛,培养学生发现、分析、解决实际问题的能力及综合应用知识的能力.因此,本研究将情景模拟教学法的设计和组织实施过程做一描述,并将此次教学改革的经验和体会与同行分享、讨论.

目的 探讨不同溶血程度对生化指标的影响,并对溶血与生化指标变化值的相关性进行回归分析.方法 用全自动生化分析仪测定大鼠的不同溶血度血清样本中磷(P)、尿酸(UA)、甘油三酯(TG)、总胆固醇(TC)、总蛋白(TP)、碱性磷酸酶(ALP)、尿素氮(BUN)、白蛋白(ALB)、血糖(GLU)生化指标值.结果 轻度溶血对生化指标测定值影响不大;中度和重度溶血对P、UA、TC、TP、ALP、BUN、ALB的指标测定值有影响,中度和重度溶血血清与正常血清生化指标测定值之间的差异有统计学意义(P＜0.05);重度溶血对TG的指标测定值有影响,重度溶血血清与正常血清生化指标测定值之间的差异有统计学意义(P＜0.05);不同程度溶血对GLU的测定值影响不大,差异无统计学意义(P＞0.05).结论 不同程度的溶血对生化常规指标值产生不同程度的影响,不同生化指标的检测结果可采用相关回归方程进行校正.

目的:探讨中西医结合治疗胫骨平台骨折的临床疗效。方法:切开复位内固定结合中医辨证用药。结果:随访8个月32例患者骨折全部愈合,临床愈合最快为13周,最迟为21周,平均为17周。半年后的膝关节功能评分优良率达到90.63%。结论:中西医结合治疗胫骨平台骨折疗效满意。

目的 优化细胞周期测定实验样品制备条件.方法 T47D细胞株经乙醇固定后,加入RNase A消化细胞内RNA,加入碘化丙啶(PI)标记DNA,用流式细胞仪检测细胞内DNA含量,ModFit软件分析细胞周期.采用此方法研究样品制备过程中固定时间(30 min,4 h,24 h,48 h,7天和14天)和RNase A对细胞周期检测结果的影响.结果 固定不同时间,G0/G1期、S期和G2/M期细胞比例变化范围分别为(57.79±0.90)%～(60.58±1.90)%,(20.16±1.55)%～(24.07±1.04)%和(17.32±0.62)%～(19.45±0.86)%,各组间差异无统计学意义(P＞0.05);变异系数的变化范围为(5.77±0.24)%～(7.72±0.28)%,各组间差异无统计学意义(P＞0.05);无RNase A组与有RNase A组相比,G0/G1期的细胞比例差异无统计学意义(t=1.59,P＞0.05),S期、G2/M期的细胞比例和变异系数的差异有统计学意义(P值均小于0.01,t值分别为-9.46,12.78和-7.99),S期升高,G2/M期降低,变异系数增大.结论 ①细胞周期测定实验样品处理过程中,细胞固定30 min后即可上机测试,且细胞固定后的保存时间可长达两周;②RNase A对细胞周期测定实验结果影响较大.

问题教学法是以学生为主体,通过创设问题来调动学生的学习兴趣和创新意识,以学生的学习活动为中心,让学生积极思考、主动实践与创新,实现从教到学的转变。在护理专业营养与食疗学的教学过程中采用问题教学法后,学生的学习积极性得到提高,综合能力得到加强。

ObjectiveTo explore the inhibiting effects of Zedoary oil on the proliferation and cathepsin K expression in A549 cell line in culture.MethodThe proliferation of lung adenocarcinoma cell line A549 were determined by MTT assay. The cells cyclic retardation were examined by using flowcytometry. Cathepsin K expression of level were detected by using western blot.ResultMTT showed that Zedoary oil had an inhibitory effect on the proliferation of A549 cells in 60~200 mg •L‐1. Flow cytometry indicated that Zedoary oil increased the percentage of G0/G1 phase cells and reduced the percentage of S phase cells. The results of western blot showed that the expression of cathepsin K was up‐regulated significangly in A549 cells in Zedoary oil groups compared with that in controls.ConclusionZedoary oil can significantly inhibit the growth of A549 cells, blocking the cell cycle. The effects may due to the up‐regulation of cathepsin K. This work was supported by founds:2008DFA31970 and the 111 project B07007 of China. Dr. Niu Jianzhao is the responsibility author.

Objective To establish the reference value of lipid metabolism indicators in serum of quail.Method To breed twenty male quails and twenty female quails with the weigh between 150 g and 200 g,which were five weeks old,in compliance with conventional,and then at different time to detect lipid metabolism indicators: detecting the triglyceride(TG) using the endpoint/GPO-PAP method,detecting the total cholesterol(TC) using the endpoint method,detecting the high density lipoprotein(HDL) and the low density lipoprotein(LDL) using the direct assay method.Result Compared with the female quail,the male quail had different values of TG,TC,HDL and LDL in serum,and the difference was statistically significant;the value of TG,TC,HDL and LDL in male quail serum was stable at different detection intervals,the reference range was determined initially and it is as follows: TG 1.15~1.55 mmol/L,TC 4.18~5.30 mmol/L,HDL 2.38~3.90 mmol/L and LDL 0.63~2.07 mmol/L.Conclusion ①The study established the reference range of lipid metabolism indicators in male quail serum.②The result indicated that the female quail in laying period as animals for lipid metabolism research is not appropriate.

基于辽宁中医药大学医学遗传学教学现状,在教学内容、教学方法、教学手段、考核方式等方面进行深入探索,建立了分层次、突重点、重操作、多考核的教学模式,提出精选合适的教学内容、提供有效的教学辅助工具和加强教师专业知识学习3项改进措施。

通过辨识兴趣培养与知识学习的辩证关系、合理协调基础知识和热点前沿的知识体系、因材施教原则确定合适的教学内容等方面对《生命科学导论》课程教学内容的创新认识,提出了灵活调整授课内容实现分层次教学、讲座式授课模式实现模块化学习、多种形式实验教学提高学生动手能力、考试方法改革实现综合素质培养目标等课程教学创新模式,并提出了多媒体和网络资源等课程实践的保障途径。

Objective To investigate the correlation between the effect of curcumin(Cur) on bleomycin-induced pulmonary fibrosis and cathepsin K expression in C57BL/6 mice.Methods The mouse model of pulmonary fibrosis was established by intratracheal injection of bleomycin.The mice were randomly divided into control group(intratracheally injecting 0.1 mL normal saline),model group(intratracheally injecting 0.025 U bleomycin) and Cur group(intratracheally injecting 0.025 U bleomycin).After 2 days control group and model group were given intragastrically 0.5% sodium carboxymethyl cellulose(CMC) saline,and Cur group,200 mg/kg·d Cur(assistant dissolving with 0.5% CMC).The samples were collected 7,14 and 28 days after modeling respectively.The lung pathological changes were observed by using HE and Mallory staining,expression of cathepsin K was detected by using immunohistochemistry assay,and difference among all groups was analyzed by using image analysis system.Results The results of HE and Mallory staining showed inflammation and fibrosis focus in varying degrees in Cur group,model group and control group,which were alleviated in Cur group compared with model group.The expression of cathepsin K increased in Cur group and model group compared with control group,and significantly increased in Cur group compared with model group(P<0.01 on the 14th day,and P<0.05 on the 7th day and 28th day.).Conclusion Cur may play its anti-fibrosis role through increasing cathepsin K expression and degrading collagen.

Objective To explore the characteristics and differences of different cell cycle analysis software for flow cytometric analysis of cell cycle.Methods Cells were stained with propidium iodide and measured by Epics XL flow cytometry.The results of cell cycle were analyzed and compared by MultiCycle and Modfit LT software.Results The percentages of cell cycle,DNA ploidy,cell proliferation and cell apoptosis were analyzed by MultiCycle and Modfit LT software,and the results were basically coincident.There were statistically significant difference in G2/G1 value of A549 cells and the proportion of Jurkat T cell aggregates between the two software(P<0.01).Conclusion For fitting results of the same sample analyzed by MultiCycle and Modfit LT software can obtain the high consistency.The G2/G1 fixed operation may be more applicable to complex composition of the sample.Although the methods of distinguishing adhesion cells and aggregate are different by different acquisition software,and lead to the aggregate ratio significant difference,but that don′t affect the results of cell cycle judgment.

为培养出既能治病救人,又具有"预防为主"思想的医疗卫生服务者,对中医院校预防医学教学进行改革,采用以案例教学为主,结合启发式教学和情景模拟教学法的教学模式,改变考核方式,调动学生的学习积极性,缩短理论知识与实际应用的距离,取得较好的教学效果。

Objective: To investigate the effects of curcumin on micronuclei formation frequency and chromosome aberration induced by cyclophosphamide. Methods: Mice were divided into six groups, the control group was treated with saline, model group was treated by intraperitoneal injection of cyclophosphamide (50mg/kg) twice every 24h before sacrifice, curcumin group was gavaged with curcumin suspension at 100mg/kg d and three treated groups were injected by cyclophosphamide combined with curcumin 50 mg/kg cyclophosphamide was given twice by intraperitoneal injection to each mouse every 24h before sacrifice. The dosage of curcumin was 200mg?kg-1?d-1, 100mg?kg-1?d-1, and 50mg?kg-1?d-1 body weight, respectively. The treatment period for all groups was 28 days. Half of all mice were given 0.04% colchicine/10g body weight 2-3h before sacrifice. The micronucleus assay in mice marrow PCE and chromosome aberration assay in mice marrow cells have been used to analyze micronuclei formation frequency and chromosome aberration rate in different groups. Results: The results showed that curcumin not only reduce the micronuclei formation frequency but also down regulate the chromosome aberration rate significantly. Conclusion: These results obtained from our experiments suggested that curcumin had protective effects against micronuclei formation and chromosome aberration.

Objective:To investigate the apoptosis inducing and antiproliferation effects of curcumin combined with bleomycin on the human type II alveolar epithelial cell line A549 in vitro.Methods:MTT was used to measure inhibitory effects of curcumin combined with bleomycin on growth of A549 cells,flow cytometry were used to analyze the cell cycle,TUNEL system and fluorescence microscope were used to observe the morphology of apoptotic cells.Results:Curcumin and bleomycin inhibited the growth of A549 in a concentration and time dependent manner,Compared with either curcumin or bleomycin alone,combining curcumin at 60μmol/L,with bleomycin at 2,4,6,8μg/mL respectively increased the growth inhibition rate of A549 cells(P0.05) significantly;curcumin induce G0/G1 inhibition or arrest in a time dependent manner,curcumin combined with bleomycin also induced apoptosis,nuclear and cytoplasmic shrinkage,chromatin condensation and apoptotic bodies can been seen.Conclusion:Curcumin combined with bleomycin inhibited the growth of A549 significantly and also induced A549 apoptosis,but only curcumin induce G0/G1 inhibition or arrest.Through changing the cell cycle phase and the apoptosis of alveolar epithelial cell,curcumin may inhibit lung fibrosis during the development after the treatment of bleomycin.

By reviewing the background of the low-grade students from medical colleges' healthy knowledge,the desire of college students to open health education courses,and the effects of opening Health Education course in Liaoning University of Traditional Chinese Medicine,this article discussed the necessity of opening College Student Health Education in medical college students.And it will provide references to other medical colleges to open analogous course.

Objective To explore the inhibiting effect of Zedoary turmeric oil on the proliferation of A549 cell line.Methods Lung adenocarcinoma cell line A549 was cultured in vitro.The inhibition rate of Zedoary turmeric oil on the proliferation of lung adenocarcinoma cell line A549 for 24,48,72 h were determined by MTT colorimetric assay.The cell cycle of lung adenocarcinoma cell line A549 stimulated by Zedoary turmeric oil for 24 h was analyzed by flow cytometry.The apoptosis and necrosis of lung adenocarcinoma cell line A549 stimulated by Zedoary turmeric oil for 24 h was tested by Annexin Ⅴ-FITC/PI assay.Results MTT assay indicated that the inhibition rate of Zedoary turmeric oil on the proliferation of lung adenocarcinoma cell line A549 increased significantly with the growing of time and concentration.Further analysis by flow cytometry indicated that Zedoary turmeric oil stimulating the A549 cells for 24 h led to G0/G1 phase arrest and blocked S phase entry.Meanwhile cells in early apoptosis,late apoptosis and necrosis were increased,and the percentage of necrotic cells was more than apoptotic cells with the increase of the concentration of Zedoary turmeric oil.Conclusion Zedoary turmeric oil can markedly inhibit the proliferation of lung adenocarcinoma cell line A549 in time-dependent and dose-dependent manners,and its role was achieved through preventing cell-cycle progression and inducing apoptosis and necrosis.