Following the publication of the above article, a concerned reader drew the Editor's attention to the fact that, regarding the EdU assay experiments shown in Fig. 6 on p. 657, discrete areas of the cells appeared to overlap in different data panels, which were intended to show differently performed experiments in two distinct cell lines (the SW1990 and Panc‑1 cell lines), albeit the areas were inverted in the case of the 'LV‑RNAi' data panels. Moreover, within these areas of overlapping cells, certain of the cells were uniquely found to be coloured differently in the associated data panels. In addition, in Fig. 9 on p. 658, the bands shown for the caspase‑3 and caspase‑3 experiments with the SW1990 cell line looked remarkably similar, suggesting that the data in this figure may also have been assembled incorrectly. After having conducted an internal investigation of the data in this paper, the Editor of International Journal of Molecular Medicine has decided that this article should be retracted from the publication on the grounds of an overall lack of confidence in the presented data. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor sincerely apologizes to the readership for any incovenience caused, and we thank the reader for drawing this matter to our attention. [International Journal of Molecular Medicine 32: 653‑660, 2013; DOI: 10.3892/ijmm.2013.1437].

对苏州大学附属第一医院普通外科2012年1月至2022年12月期间诊断Petersen疝并行手术治疗的11例患者的临床资料进行回顾性分析。男9例，女2例，发病年龄48~75岁，2例为外院行开腹胃癌根治术，9例为本院行胃癌根治术，其中5例为开腹手术，4例为腹腔镜手术；8例为全胃切除术，2例为远端胃次全切除术，重建方式均为Roux-en-Y吻合，1例为近端胃次全切除术，重建方式为双通道吻合；发生Petersen疝时距离接受胃癌手术的时间为10 d~45.8个月，中位时间12.5个月，Petersen疝术后住院天数4~19 d，手术时间为55~209 min，中位时间为105 min，1例死亡，10例好转。本研究显示Petersen疝是胃癌术后少见并发症，不能自愈，需尽早诊断和手术治疗。

Objective:To explore the necessity of salvage surgery after endoscopic resection of early rectal cancer.Methods:The clinical data of 62 patients who underwent salvage surgery for early rectal cancer at the First Affiliated Hospital of Soochow University from Jan 2012 to Dec 2021 were retrospectively analyzed.Results:Six cases (10%) had residual tumors, 8 cases (13%) had lymph node metastasis and one case (2%) had cancer nodules; The operation time was (178±82) mins; 1 case (2%) developed anastomotic lerakage, which was cured; 2 cases (3%) developed intestinal obstruction, of which 1 case (2%) relieved on conservative management and 1 case (2%) underwent reoperation.Conclusion:It is necessary to perform salvage surgery for early rectal cancer patients with residual caner and risk factors of lymph node metastasis after endoscopic therapy.

[This retracts the article DOI: 10.2147/OTT.S66809.].

BackgroundThe intestinal flora is correlated with the occurrence of colorectal cancer. We evaluate a new predictive model for the non-invasive diagnosis of colorectal cancer based on intestinal flora to verify the clinical application prospects of the intestinal flora as a new biomarker in non-invasive screening of colorectal cancer.MethodsSubjects from two independent Asian cohorts (cohort I, consisting of 206 colorectal cancer and 112 healthy subjects; cohort II, consisting of 67 colorectal cancer and 54 healthy subjects) were included. A probe-based duplex quantitative PCR (qPCR) determination was established for the quantitative determination of candidate bacterial markers.ResultsWe screened through the gutMEGA database to identify potential non-invasive biomarkers for colorectal cancer, including Prevotella copri (Pc), Gemella morbillorum (Gm), Parvimonas micra (Pm), Cetobacterium somerae (Cs), and Pasteurella stomatis (Ps). A predictive model with good sensitivity and specificity was established as a new diagnostic tool for colorectal cancer. Under the best cutoff value that maximizes the sum of sensitivity and specificity, Gm and Pm had better specificity and sensitivity than other target bacteria. The combined detection model of five kinds of bacteria showed better diagnostic ability than Gm or Pm alone (AUC = 0.861, P < 0.001). These findings were further confirmed in the independent cohort II. Particularly, the combination of bacterial markers and fecal immunochemical test (FIT) improved the diagnostic ability of the five bacteria (sensitivity 67.96%, specificity 89.29%) for patients with colorectal cancer.ConclusionFecal-based colorectal cancer-related bacteria can be used as new non-invasive diagnostic biomarkers of colorectal cancer. Simultaneously, the molecular biomarkers in fecal samples are similar to FIT, have the applicability in combination with other detection methods, which is expected to improve the sensitivity of diagnosis for colorectal cancer, and have a promising prospect of clinical application.

Yes-associated protein (YAP), as a major downstream effector in the Hippo signaling pathway, is considered as an oncogene in cancer. The present study aimed to investigate the potential role of YAP in the development and progression of colorectal cancer (CRC). The mRNA and protein expression levels of YAP in human CRC tissue samples and adjacent normal tissue were analyzed using public databases, as well as clinical samples. The potential roles of YAP and the underlying mechanism regulating the proliferation and migration of CRC cells were examined using genetic manipulation in vitro. The correlation between the expression of the YAP gene and epithelial-to-mesenchymal transition (EMT) markers was investigated in order to determine the mechanism underlying the observed effects of YAP. YAP mRNA expression levels were significantly upregulated in CRC tissue compared with in normal tissue, as determined using datasets obtained from Oncomine. Similarly, in clinical samples, the protein expression levels of YAP were significantly upregulated in CRC tissue samples compared with in normal tissue samples. YAP knockdown inhibited the proliferation and migration of CRC cells in vitro, whereas its overexpression resulted in the opposite effect. The expression levels of the YAP gene were positively correlated with those of EMT markers (such as vimentin and N-cadherin) and EMT-inducing transcription factors (such as Snail1, Slug and zinc finger E-box binding homeobox 1 and 2) in CRC samples from Gene Expression Profiling Interactive Analysis. Furthermore, YAP silencing increased the protein expression of E-cadherin and decreased that of vimentin in CRC cells. By contrast, the overexpression of YAP had the opposite effect. YAP promoted the glucose transporter 3 (Glut3)/AMP-activated protein kinase (AMPK) signaling pathway in CRC cells. In conclusion, YAP promoted the proliferation and migration of CRC cells, as well as the expression of EMT markers, possibly by regulating the Glut3/AMPK signaling pathway.

目的 研究早期远端胃印戒细胞癌淋巴结转移的危险因素,进一步分析其外科手术指征.方法 回顾性分析2013年3月至2018年11月期间在苏州大学附属第一医院普外科接受外科根治手术且术后病理学检查证实为远端胃印戒细胞癌的91例早期胃癌患者的临床资料,收集患者的性别、年龄、肿瘤最大径、病灶数量、浸润深度、肿瘤大体外观、脉管癌栓、合并溃疡等数据,探索发生淋巴结转移的危险因素,进一步分析外科手术指征.结果 91例早期远端胃印戒细胞癌均接受了外科根治性手术,其中淋巴结转移10例.单因素分析结果显示,肿瘤最大径(x2=5.631,P=0.025)、浸润深度(x2=4.389.P=0.016)、病灶数量(x2=5.615,P=0.023)及脉管癌栓(x2=22.500.P=0.001)均与早期远端胃印戒细胞癌的淋巴结转移有关.多因素分析结果显示,肿瘤最大径(OR=3.675.P=0.012)、浸润深度(OR=3.886,P=0.015)及脉管癌栓(OR=8.711.P＜0.001)是早期远端胃印戒细胞癌发生淋巴结转移的影响因素,肿瘤最大径≥2 cm、浸润至黏膜下层及有脉管癌栓的患者有更高的淋巴结转移率.结论 肿瘤最大径≥2 cm、浸润至黏膜下层及存在脉管癌栓的早期远端胃印戒细胞癌患者有更高的淋巴结转移风险;满足肿瘤最大径≥2 cm和存在脉管癌栓中任何1项条件者均可能需接受外科根治性手术.

目的 探讨腹腔镜下腹股沟疝修补术对手术效果及T淋巴细胞百分比的影响.方法 选取2017年1月-2019年1月腹股沟疝患者280例作为研究对象,随机分为对照组(n=140)和观察组(n=140).对照组采用开放无张力疝修补术,观察组采用腹腔镜下无张力疝修补术,术后7 d对患者治疗效果进行评估,比较两组手术指标、疼痛视觉模拟评分(VAS)、T淋巴细胞百分比及术后并发症.结果 观察组与对照组手术时间比较,差异无统计学意义(P>0.05);观察组出血量、术后自主活动时间及VAS评分少于对照组(P<0.05);观察组治疗费用多于对照组(P<0.05);观察组手术后7 d CD3+、CD4+和CD4+/CD8+百分比高于对照组(P<0.05);观察组手术后7 d CD8+百分比低于对照组(P<0.05);观察组手术后切口疼痛、尿潴留、阴囊水肿及切口感染发生率与对照组比较,差异均无统计学意义(P>0.05).结论 将腹腔镜手术用于腹股沟疝患者中,手术创伤较小,能改善患者T淋巴细胞百分比,未增加术后并发症发生率,值得推广应用.

目的 比较奥沙利铂联合替吉奥或卡培他滨治疗结直肠癌的效果.方法 选取2017年1月-2017年12月在医院胃肠外科手术治疗后的结直肠癌患者86例,随机分为观察组和对照组各43例,观察组采用替吉奥联合奥沙利铂方案(SOX方案)治疗,对照组采用卡培他滨联合奥沙利铂方案(XELOX方案)治疗,比较2组患者的临床疗效、不良反应、1年生存率及中位生存期.结果 2组客观有效率和疾病控制率比较差异均无统计学意义(P＞0.05).2组消化道反应、骨髓抑制、肝肾功能异常、口腔黏膜炎、色素沉着、手足综合征等不良反应发生率及严重程度比较差异均无统计学意义(P＞0.05).2组1年生存率及中位生存期比较差异均无统计学意义(P＞0.05).结论 奥沙利铂联合替吉奥或卡培他滨治疗结直肠癌的效果相当,SOX方案为临床治疗结直肠癌提供了新的选择.

目的 观察腹腔镜免输精管分离腹股沟疝修补术的临床疗效.方法 成年男性腹股沟疝患者125例,随机分为观察组(62例)和对照组(63例),观察组采用腹腔镜免输精管分离腹股沟疝修补术,对照组采用腹腔镜经腹腔腹膜前腹股沟疝修补术,回顾性比较两组手术相关资料.结果125例手术均顺利完成.观察组单侧及双侧手术时间均短于对照组[(0.97±0.20)h v s.(1.80±0.21)h和(1.21±0.17)h vs.(2.01±0.30)h](P<0.05).观察组术后阴囊血清肿发生率低于对照组(P<0.05).两组术中出血量、术后住院时间、局部腹股沟血肿、尿潴留、慢性疼痛等均无统计学差异(P>0.05).结论 与腹腔镜经腹腔腹膜前腹股沟疝修补术相比 ,腹腔镜免输精管分离腹股沟疝修补术可更好地保护生殖成分 ,不增加并发症及复发率.

目的:对比用腹腔镜结肠癌根治术与开腹结肠癌根治术治疗结肠癌的效果.方法:将2017年3月至2018年2月期间苏州大学附属第一医院收治的80例结肠癌患者随机分为OBG组和REG组.对OBG组患者进行腹腔镜结肠癌根治术,对REG组患者进行开腹结肠癌根治术.然后,对比两组患者的各项临床指标.结果:与REG组患者相比,OBG组患者术中的出血量较少,其术毕至肠鸣音恢复的时间、术毕至肛门排气的时间及住院的时间均较短,其术后并发症的发生率较低,其感情职能、生理功能、躯体功能和社会功能的评分均较低,P<0.05.结论:与进行开腹结肠癌根治术相比,对结肠癌患者进行腹腔镜结肠癌根治术的效果更好,可减少其术中的出血量,促进其术后的康复,降低其术后并发症的发生率,提高其生活质量.

目的 比较腹腔镜手术与开腹结肠癌根治术对结肠癌患者感染率及肠道菌群的影响.方法 回顾性分析2017年7月-2018年6月医院收治的90例结肠癌患者的病例资料,按照治疗方法分为腹腔镜手术组48例和开腹手术组42例.比较两组术后感染率;患者术前、术后5d粪便菌群数量;术前、术后1d、3d、5d血清白细胞介素-6 (IL-6)、肿瘤坏死因子-α(TNF-α)、C-反应蛋白(CRP)水平.结果 腹腔镜手术组术后感染率为4.16％,低于开腹手术组的16.66％(P＜0.05).两组患者术后1d、术后3d、术后5d血清IL-6、TNF-α、CRP水平较术前升高(P＜0..5),腹腔镜手术组患者血清IL-6、TNF-α、CRP水平低于开腹手术组患者(P＜0.05).术后5d腹腔镜手术组肠道大肠埃希菌、肠球菌低于开腹手术组,乳酸杆菌、双歧杆菌属、双歧杆菌/大肠埃希菌高于开腹手术组(P＜0.05).结论 相对开腹结肠癌根治手术,腹腔镜结肠癌根治手术患者术后炎症反应较轻,对肠道菌群影响较小,术后感染发生率也较低.

Background: Effective gastric carcinoma (GC) chemotherapy is subject to many in vitro and in vivo barriers, such as tumor microenvironment and multidrug resistance. Materials and methods: Herein, we developed a hyaluronic acid (HA)-modified silica nanoparticle (HA-SiLN/QD) co-delivering quercetin and doxorubicin (DOX) to enhance the efficacy of GC therapy (HA-SiLN/QD). The HA modification was done to recognize overexpressed CD44 receptors on GC cells and mediate selective tumor targeting. In parallel, quercetin delivery decreased the expression of Wnt16 and P-glycoprotein, thus remodeling the tumor microenvironment and reversed multidrug resistance to facilitate DOX activity. Results: Experimental results demonstrated that HA-SiLN/QD was nanoscaled particles with preferable stability and sustained release property. In vitro cell experiments on SGC7901/ADR cells showed selective uptake and increased DOX retention as compared to the DOX monodelivery system (HA-SiLN/D). Conclusion: In vivo anticancer assays on the SGC7901/ADR tumor-bearing mice model also revealed significantly enhanced efficacy of HA-SiLN/QD than mono-delivery systems (HASiLN/Q and HA-SiLN/D).

Fatty acid synthase (FASN), the main enzyme involved in de novo lipogenesis, is overexpressed in several types of tumor tissues. In addition, it is associated with tumor cell proliferation, metastasis, epithelial-mesenchymal transition (EMT) and a poor prognosis. However, the precise functions and internal mechanisms of FASN with regard to the proliferation, metastasis and EMT in gastric cancer (GC) cells remain elusive. The present study investigated FASN protein expression in 18 randomly selected pairs of GC tumors and matched normal tissues by western blot analysis. FASN-specific small interfering RNA (siRNA) was then transfected into SGC-7901 cells to examine the effect of FASN on proliferation and migration in vitro. Western blotting was used to detect the protein expression of FASN, EMT-related markers and key signaling molecules of the mechanistic target of rapamycin/zinc finger protein GLI1 (mTOR/Gli1) pathway. Reverse transcription-quantitative polymerase chain reaction was conducted to detect the mRNA expression of FASN and EMT-related markers. The FASN level was higher in the GC tissues compared with that in the surrounding normal tissues. Knockdown of FASN suppressed GC cell proliferation and metastasis in vitro. The silencing of FASN expression using siRNA reversed EMT at the protein and mRNA levels and decreased the expression of Gli1 via regulation of AMP-activated protein kinase/mTOR and protein kinase B/mTOR signaling in GC cells. Inhibition of FASN suppresses GC proliferation and metastasis through targeting of the mTOR/Gli1 signaling pathway, indicating that it may serve as a potential target for the treatment of GC.

目的 探讨腹腔镜手术与传统开腹手术治疗老年结肠癌患者对术后生理应激、功能恢复以及炎症因子的影响.方法 82例结肠癌老年患者按照随机分配原则分成对照组与观察组,分别应用传统开腹手术与腹腔镜下根治手术,对比两组患者的手术效果以及生理应激、功能恢复、炎症因子水平差异.结果 观察组患者手术时间、术中出血量以及手术切口长度均明显低于对照组(P<0.05),肿瘤坏死因子(TNF)-α、白介素(IL)-6、C反应蛋白(CRP)水平均低于对照组(P<0.05),凝血功能障碍及认知功能障碍的发生率少于对照组(P<0.05),且观察组患者并发症发生率更低.结论 老年结肠癌患者应用腹腔镜手术治疗效果显著优于传统开腹手术,减少了手术风险,有利于术后恢复;同时,腹腔镜手术能够降低炎症因子水平,降低术后并发症发生率,提高生活质量.

目的 探讨自发性气腹常见病因、临床表现、影像学表现、诊断及治疗策略.方法 结合国内外文献,对2013年11月—2017年5月就诊的6例自发性气腹患者的临床资料进行综合性分析.结果 3例行手术治疗,术中均未发现明显消化道穿孔部位,其中1例考虑与自发性细菌性腹膜炎有关,术后好转,1例与肠气囊肿症有关,术后好转,1例与肠源性感染致肠道积气明显,气体经菲薄肠壁渗入腹腔有关,于术后2周死亡;3例经非手术治疗好转,1例考虑与胆道感染后致胆道积气明显有关,1例与肝脓肿破裂有关,1例与高压气枪损伤肛门后致皮下广泛积气,气体经筋膜间隙蔓延至腹腔有关.结论遇到存在气腹征象的急腹症时需警惕自发性气腹可能,需通过详细询问病史、仔细查体、认真影像学判读等手段综合分析后做出正确的临床判断,切莫一见气腹即行剖腹探查.

Objective To observe clinical efficacy and explore clinical value of a modified procedure of double-stapling technique for mid-low rectal cancer.Methods Clinical data of patients undergoing laparoscopic anterior resection at the Department of General Surgery,the First Affiliated Hospital of Soochow University from February 2011 to February 2015 was analyzed retrospectively.According to the different ways in doing double-stapling technique,we divided patients into modified group (51 cases) and conventional group (74 cases).Parameters were compared between the two groups as general considerations,oncologic outcomes.Data were analyzed by SPSS 17.0 software packet,using t and x2 inspection.Results The difference of the general data of two groups was not statistically significant (P ＞ 0.05).Operation time in the modified group was longer than that of the conventional group [(169 ± 23) vs.(150 ±42)min,t =-3.150,P ＜0.05],but it had shorter drainage tube indwelling days [(7.9 ±2.9)d vs.(10.8±11.6)d,t=1.999,P＜0.05] and length of hospital stay after surgery [(10.0±3.6)d vs.(13.3 ± 13.7) d,t =1.025,P ＜ 0.05].The incidence of anastomotic leakage (2.0％ vs.18.9％,x2 =4.402,P ＜ 0.05) and tenesmus(3.9％ vs.17.6％,x2 =4.110,P ＜ 0.05) in the modified group was less than that of the conventional group.The difference in those areas was not statistically significant (P ＞ 0.05),such as intraopretive blood loss,per-anal exhaust time,consumption of liquid diet time,anastomotic bleeding,intestinal obstruction,reoperation for neostomy and infections.Conclusions Compared with traditional laparoscopic anterior resection,"End-Corner" anastomosis has the benefits of less postoperative anastomotic leakage and fewer" low anterior resection syndrome".

Objective To investigate the effect of T lymphocyte function by silencing decoy receptor 3 (DcR3) in human Pancreatic cancer cell line Panc-1.Methods Small interfering RNA (siRNA)eukaryotic expression vector targeting DcR3 gene was transfected into Panc-1 cells by Lipofectamine 2000.The expression of DcR3 was detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA) after transfection 48 h.After mixed lymphocyte tumor cell cultured for 72 h,lymphocyte subsets were examined by flow cytometry analysis and the level of interferon (IFN)-γ,interleukin (IL)-4 was determined by ELISA.Results In the DcR3-siRNA transfection group,the expression of DcR3 was decreased in Panc-1 cells remarkably.After Panc-1 cells silencing DcR3 in mixed lymphocyte tumor cell cultured,the number of CD3 +,CD4 + T lymphocyte was increased (P ＜ 0.05) and the number of CD8 + T lymphocyte was unchanged statistically (P ＞ 0.05).Additionally,the number of Th1 cells was increased [(7.87 ± 1.07) ％ vs.(5.57 ± 0.70) ％,P ＜ 0.05],while the number of Th2 cells was decreased [(0.91 ± 0.15) ％ vs.(2.83 ± 0.21) ％,P ＜ 0.05] as compared with negative control group (NC-siRNA).Conclusion The silencing DcR3 may reverse immune regulating of T lymphocyte and Correct the imbalance of Th1/Th2.

目的：对比研究单极电凝联合 Ligasure 与超声刀两者在腹腔镜远端胃癌根治术（LADG）中临床疗效的差异，为单极电凝联合Ligasure临床适用性进一步奠定基础。方法：回顾性分析2012年1月至2014年1月我院112例LADG的临床资料，其中观察组53例以单极电凝联合Ligasure 为主要操作器械，对照组59例以传统超声刀为主要操作器械，比较两组手术时间、术中出血量、淋巴结清扫数目、术后引流液量、住院时间、胃肠道功能恢复时间、术后并发症方面的差异。结果：观察组手术时间明显短于对照组[（131±47．2）min vs．（151±51．8）min，P ＝0．018]；观察组术中出血量明显少于对照组[（108．2±43．9）mL vs．（188．4±51．7）mL，P ＝0．026]；观察组术后引流液量明显少于对照组[（216±68）mL vs．（272±76）mL，P ＝0．041]；两组在淋巴结清扫数目、住院时间、胃肠道功能恢复时间、术后大出血以及肺部感染方面比较差异无统计学意义。结论：以单极电凝联合Ligasure行LADG是安全、可行的，且具有缩短手术时间，减少术中出血的优势。

目的 分析影响胰体尾切除术后并发症发生的危险因素.方法 回顾性分析了78例行胰体尾切除术患者的临床资料,分析术后并发症的发生情况及其影响因素.结果 术后24例(30.8％)患者出现术后并发症,其中死亡1例.术前空腹血糖水平、术中出血量和输血以及结扎主胰管与术后并发症的发生密切相关(P＜0.05).术前空腹血糖水平和结扎主胰管是胰体尾切除术后并发症发生的独立危险因素(P＜0.05).结论 为了降低胰体尾切除术后并发症的发生,应重视针对高危因素采取相应的预防措施.