This study explores the mechanism of miR-19b-3p in bladder cancer (BCa) cell proliferation and apoptosis to provide the latest theoretical basis for miR-19b-3p to become a novel biomarker and therapeutic target for BCa. miR-19b-3p, lncRNA SNHG20, and HS3ST3B1 expressions in BCa tissues or cells were detected via RT-qPCR or Western blot. Cell proliferation was evaluated via CCK-8 and colony formation assays. Cell apoptosis was assessed via flow cytometry, and apoptosis related factors Bax and Bcl-2 were detected via Western blot. Dual luciferase and RIP assays confirmed the binding of miR-19b-3p and lncRNA SNHG20. The binding between lncRNA SNHG20, TARDBP, and HS3ST3B1 was analyzed by RIP, RNA pull down, and co-immunoprecipitation. The RNA stability of lncRNA SNHG20 and HS3ST3B1 was tested after actinomycin D treatment. A nude mouse xenograft tumor model was established to validate the effect of miR-19b-3p on BCa in vivo. miR-19b-3p was weakly expressed in BCa, while lncRNA SNHG20 and HS3ST3B1 were highly expressed. Overexpression of miR-19b-3p repressed BCa cell proliferation but facilitated apoptosis. Mechanistically, miR-19b-3p decreased lncRNA SNHG20 expression by binding to lncRNA SNHG20 and reducing its stability, thus repressing the interaction between lncRNA SNHG20-TARDBP-HS3ST3B1. Further in vivo experiments also revealed that miR-19b-3p restrained the in vivo tumorigenicity of BCa cells and promoted apoptosis by suppressing the lncRNA SNHG20/HS3ST3B1 axis. In conclusion, overexpression of miR-19b-3p represses BCa cell proliferation and promotes apoptosis by suppressing the lncRNA SNHG20/HS3ST3B1 axis.

Objective:To investigate the effects of gemcitabine on the proliferation, cell cycle, tumor invasion and tumor stem cell characteristics of bladder cancer stem cell-like cells.Methods:Bladder cancer stem cell-like cell lines were selected by using the chemotherapy drug cisplatin combined with serum-free medium technology, CCK-8 method was used to detect the inhibitory effect of different concentrations of gemcitabine on bladder cancer stem cell-like cells, The cell cycle distribution of bladder cancer stem cell-like cells was detected by flow cytometry, qRT-PCR and Western blot were used to detect the expression of ctamer binds transcription factor 4(OCT-4) and human balanced nucleoside transporter 1(hENT-1) in the experimental and control groups.Results:After treatment with 1.6 μmol/L gemcitabine for 24 h, the cell inhibition rate was the highest [(75.2±4.5) %, P<0.001]. Compared with each group, the number of cells in S phase in group C decreased significantly, while the expression of hENT-1 protein was significantly increased ( P<0.001). The tumor invasion ability of bladder cancer stem cell-like cells treated with gemcitabine was decreased ( P<0.001). Conclusions:Gemcitabine has a killing and inhibitory effect on bladder cancer stem cell-like cells, which can weaken the stem cell characteristics and tumor invasion ability.

Objective:To investigate the expression of DLG1 in bladder cancer and its relationship with clinicopathological features and prognosis.Methods:The expression levels of DLG1 in the bladder cancer tissues and normal bladder tissues collected from 138 cases of bladder cancer patients from February 2009 to September 2014 were detected by real-time fluorescence reverse transcription and immunohistochemistry, and its correlation with clinicopathological features and prognosis was analyzed.Results:The mRNA and protein expression levels of DLG1 of bladder cancer group was higher than that in normal bladder group ( P<0.01). The positive rate of DLG1 protein expression in bladder cancer was higher than that in normal bladder group ( P<0.01). The expression level of DLG1 protein was not significantly correlated with age ( P>0.05), while was positively correlated with TMN stage, pathological grade, recurrence, depth of invasion, vascular infiltration, maximal diameter of tumor (≥2 cm) and lymph node metastasis ( P<0.01). The 3-year survival rate and survival time of the DLG1 negative group were significantly higher than those of the DLG1 positive group ( P<0.01). Conclusions:The expression of DLG1 is elevated in bladder cancer patients, and it can promote the disease development, so patients with low expression of DLG1 can obtain a better prognosis.

1Urology Department, The Affiliated Cancer Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, People’s Republic of China; 2The 1st Nephrology Department, Heilongjiang Academy of Traditional Chinese Medicine, Harbin, Heilongjiang 150036, People’s Republic of China; 3Urology Department, The 1st Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang 150040, People’s Republic of China; 4Interventional Department, The Affiliated Cancer Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, People’s Republic of China

Background Long noncoding RNAs (lncRNAs) play critical and complex roles in regulating various biological processes of cancers. Our study aimed to investigate the involvement of lncRNA NCK1-AS1 in urinary bladder cancer (UBC). Methods qRT-PCR was used to detect the expression of lncRNA NCK1-AS1 and miR-143 in UBC tissues and cells. The dual-luciferase reporter system assays were used to confirm the interaction between NCK1-AS1 and miR-143, and flow cytometry assays were applied to examine the behavioral changes in HT-1376 and HT-1197 cell lines. Results It was observed that NCK1-AS1 was up-regulated, while miR-143 was down-regulated in tumor tissues than in adjacent healthy tissues of urinary bladder cancer (UBC) patients. A 5-year survival analysis showed that the survival rate of patients with high NCK1-AS1 level or low miR-143 level in tumor tissues appears relatively low. Correlation analysis revealed a significant inverse correlation between NCK1-AS1 and miR-143 in tumor tissues. Over-expression NCK1-AS1 reduced the expression level of miR-143, while elevating the level of miR-143 failed to affect NCK1-AS1 expression. NCK1-AS1 over-expression led to promoted proliferation and increased percentage of CD133+ (stemness) cells. Conclusion Therefore, NCK1-AS1 promotes cancer cell proliferation and increases cell stemness in UBC patients by down-regulating miR-143.

Clear-cell renal cell carcinoma (ccRCC) is an aggressive and malignant kidney cancer which has the worst prognosis. Although microRNAs (miRNAs) have recently been identified as a novel class of regulators in oncogenesis and metastasis, there are few studies on their participation in ccRCC. In the present study, we observed that miR-367 expression was increased in both human ccRCC tissues and cell lines. Cell proliferation was evaluated by MTT assay and 5-Ethynyl-2'-deoxyuridine (EdU) assay kit, which indicated that inhibition of miR-367 could suppress the ccRCC proliferation. Forced expression of miR-367 substantially induced cell migration and invasion evidenced by wound-healing and transwell assays, and this carcinogenesis could be abolished by miR-367 inhibitor treatment. Further analysis identified Metastasis-Associated Protein 3 (MTA3) as a direct target of miR-367. QRT-PCR and western blot results indicated the correlative expression of miR-367 and MTA3 in ccRCC tissue samples. Overexpression of MTA3 reversed miR-367-induced cell proliferation, migration and invasion. Our data uncovered a novel molecular interaction between miR-367 and MTA3, indicating a therapeutic strategy of miR-367 for ccRCC.

Objective: To report the successful experience of the laparoscopic radical nephrectomy after neoadjuvant targeted therapy implemented to patients with the renal carcinoma complicated with vena cava tumor thrombus, followed by an exploration of the efifcacy of neoadjuvant targeted therapy and the therapeutic schedule.Methods: A 52 year old woman who was examined of right renal lesion for one month through the physical examination was hospitalized in our hospital in September 2012. ThePET-CT showed an 11.9 cm×8.1 cm tumor in the middle and lower part of the right kidney. It was veriifed as the renal carcinoma complicated with the vena cava tumor thrombus (Mayo II).Percutaneous biopsy conifrmed it clear cell carcinoma of kidney. Sunitinib 4/2 with 50 mg a day was used for patients for eleven weeks. 2 weeks after suspending, laparoscopic radical nephrectomy was implemented to the patient.Imaging evaluation was performed again before the operation.The follow-up survey was carried out.Results:After neoadjuvant treatment, CT scan revealed 27.73% size reduction of renal tumor, with only 8.6 cm×5.4 cm. Intravenous tumor embolus was obviously reduced (Mayo I).According to RECIST criteria, the objective response was partial remission of disease(PR).Nephrectomy was successfully performed under general anesthesia.Pathology report revealed that renal clear cell carcinoma was with major necrosis in primary tumor. During one year of post-operative follow-up, there was no recurrence and metastasis.Conclusion:Neoadjuvant Sunitinib treatment could result in the shrinking of primary tumor and tumor thrombi of vena, reducing the risk of surgery and improving the success rate of surgery, thus eventually improving patients overall survival rate.

Objective To investigate the clinical value and adverse reactions of indwelling ureteral stentsinthepreventionofureteralinjurybeforecomplexpelvictumorsurgery.Methods 145casesofpatients with rectal cancer ,cervical cancer ,ovarian cancer and pelvic sarcomas were retrospectively analyzed ,and 53 pa-tients with complex pelvic tumor surgery ,preoperative were under cystoscope unilateral or bilateral ureteral stent tube,pulled out according to the intraoperative situation after surgery or lien ,92 patients as control group .Results Ureteral injury was found in 10 of the 145 patients,2 cases in ureteral catheter group and 8 cases in control group .3 cases of postoperative ureteral fistula occurred in the control group .Indwelling ureteral stents could cause adverse reactions such as hematuria ,osphyalgia and urinary irritation ,and the adverse reactions of catheter group was obviously higher than that of control group (P<0.05).Conclusion Cystoscopic ureteral stent placement has important clinical significance for prevention of ureteral injury despite certain adverse reactions ,which can be used in operation of complex pelvic tumor .

目的:探讨肾癌患者异常增高的凝血指标、静脉血栓栓塞症(VTE)发生率与临床分期的关系,分析肾癌患者血液高凝状态的临床特征.方法:对2010年至2012年335例住院的肾癌患者Fib、D-D、BPC水平进行检测并筛查发生VTE的患者.结果:335例肾癌患者中,异常Fib、D-D、BPC的发生率分别为20.6％、9.9％、6.6％,且随着肾癌分期的增加,凝血指标异常的发生率逐渐升高,各分期之间比较有统计学差异(P＜0.05);VTE的发生率为2.2％,Ⅳ期肾癌患者最高为1.2％,且各分期之间比较有统计学意义(P＜0.05).年龄≥60岁、肿瘤最大径＞10 cm、临床分期晚、伴有远处转移的肾癌患者血液高凝的发生率分别显著高于年龄＜60岁、肿瘤最大径＜10 cm、临床分期早、不伴有远处转移的肾癌患者(P＜0.05).结论:年龄≥60岁、肿瘤最大径＞10 cm、临床分期晚、伴有远处转移可能是肾癌患者发生血液高凝状态和VTE的危险因素,应引起临床重视.

Objective To evaluate the influence and significance of Pingyangmycin chemotherapy on the c-myc and Ras-P21 protein expression in penile cancer .Methods A total of 100 penile squamous cell carci-noma cases was retrospectively studied and divided into two groups .Data were obtained from 1995 to 2005 .In the chemotherapy group ,50 cases of patients were selected to perform preoperative chemotherapy before surgery .The patients were treated by Pingyangmycin .After 7 times of medication ,partial excision of penis plus improved ingui-nal lymph node dissection was performed .In the control group ,50 cases of patients were selected for partial exci-sion of penis plus improved inguinal lymph node dissection directly without any pre -operative chemotherapy .All pathology specimens were detected of c -myc and Ras-P21 protein expression by immunohistochemical staining assay.Theχ2 test was used for the statistical analysis .Results In chemotherapy group,the positive expression rates of c-myc and Ras-P21 were 30%,27%,respectively.However,in control group,the positive expression rate of c-myc,Ras-P21 were 52%,48%,respectively.By theχ2 test,the expressive differences of c -myc,Ras-P21 positive expression rate between chemotherapy group and control group were all significant (P<0.05). Conclusion The protein expressions of c -myc and Ras-P21 is significantly decreased in the tissue of Pingy-angmycin chemotherapy of penile cancer .

Dendritic cells (DCs) play an important role in anti-renal cell carcinoma (RCC) immunity. The aim of the study was to investigate effect of mimic RCC microenvironment on phenotype and function of DCs. We isolated conditioned media (CM) from supernatants of culturing RCC cells and adjacent non-RCC cells in patients. CD14+ monocytes were obtained from healthy donors. The monocytes derived DCs were treated by RCC CM and non-RCC CM. Maturation markers CD80, CD83, CD86, and HLA-DR on DCs were analyzed using flow cytometry, while the levels of IL-10, TGF-β, and IL12p70 in supernatants were examined by ELISA. The DCs migration treated with RCC CM and non-RCC CM was investigated using transwell assay. The DCs treated and allogenic T cells were co-cultured for detecting T-cell proliferation and change of phenotype on the T cells. Our results indicated that RCC CM inhibited the up-regulation of CD80,CD83, CD86, and HLA-DR in response to LPS in treated DCs and increased IL-10 and TGF-β secretion but reduced IL12p70 production. Moreover, the migration ability of DCs treated with RCC CM was also inhibited, compared to DCs treated with adjacent non-RCC CM. In addition, T-cell proliferation was suppressed in co-culture assay with DCs treated with RCC CM; proportion CD25+Foxp3+ regulatory T cells were induced to increase. This study suggests that RCC CM can inhibit maturation of DCs and impair its function; moreover, DCs treated with RCC CM induce regulatory T cells increase, thus could contribute RCC escape from antitumor immunity.

Ribavirin is an anti-viral drug; however, recent data suggest that it may also be effective in cancer therapy. This study investigated the effect of ribavirin alone or in combination with IFN-α on biological processes: proliferation, apoptosis, and migration of murine (Renca) and human renal carcinoma (RCC) cells (786–0) in vitro.

目的 探讨静脉应用维库溴铵在预防膀胱侧壁肿瘤行经尿道肿瘤电切术(TURBt)中发生闭孔神经反射的可行性.方法 统计哈尔滨医科大学附属肿瘤医院自2009年1月-2012年9月行经尿道膀胱肿瘤电切术的109例患者,其中采用静脉麻醉配合喉罩通气,同时应用肌松药物维库溴铵患者65例(静脉麻醉组),采用连续硬脊膜外麻醉者44例(连续硬膜外麻醉组),观察两组患者的一般情况、闭孔神经反射情况、膀胱穿孔、出血量情况、是否中转开放手术、以及术后留置导尿管时间情况.结果 静脉麻醉组与连续硬膜外麻醉组的一般情况差异无统计学意义.静脉麻醉组中,有5例于术中发生了极其轻微的下肢抖动,其余60例患者手术全程未发生闭孔神经反射;连续硬脊膜外麻醉组中,发生闭孔神经反射29例.与硬膜外组比较,静脉麻醉组中闭孔神经反射的发生率明显降低(P＜0.01).结论 相对于连续硬膜外麻醉而言,全身麻醉同时静脉应用维库溴铵配合喉罩通气是一种简单、安全、有效的预防膀胱侧壁肿瘤电切术中发生闭孔神经反射的方法.

Objective:To observe the curative effect of the lactase preparations for medical on rotavirus enteritis with secondary lactose intolerance.Methods:The children who were suffering from rotavirus enteritis with secondary lactose intolerance were randomly divided into experimental and control groups,the experimental group were treated with lactase,then efficacy and course of treatment were observed.Results:The total effective rates of the two groups were significantly different(P0.005),the experimental group was significantly higher than the control group;The courses of treatment of the two groups were significantly different(P0.01),the experimental group was significantly shorter than the control group.Conclusions:Application of the lactase preparations for medical to treat rotavirus enteritis with secondary lactose intolerance can improve the efficacy,and shorten the course of treatment.

Objective To investigate the relationship between the serum interleukin-18(IL-18) and type 2 diabetes mellitus with macroangiopathy.Methods The levels of serum IL-18 were determined by enzyme linked immunosorbent assay(ELISA) in 30 healthy controls and 60 cases with type 2 diabetic patients(including 30 cases with macroangiopathy and 30 cases without complications).Other clinical parameters were also measured.Results The levels of IL-18,triglyceride(TG),blood pressure(BP) were markedly elevated in type 2 diabetic macroangiopathy group than in type 2 diabetes mellitus without complications and normal control group.IL-18 was correlated positively with BP,body mass index(BMI),TG,homeostasis model of assessment for insulin resistence index(HOMA-IR),course,fasting plasma glucose(FPG).Conclusion The levels of IL-18 are markedly elevated in type 2 diabetes mellitus especially in type 2 diabetes mellitus with macroangiopathy.It suggests that inflammation plays animportant role in the development of type 2 diabetes mellitus and macroangiopathy.IL-18 may be one risk factor and predictor for type 2 diabetic macroangiopathy.

Objective: To study the Low density lipoprotein receptor-related protein in tumor in acute leukemia expression situation. Methods: case of illness group will gather36 examples clinical first diagnoses forthe acute leukemia patient's marrow, the lymphocyte separation fluid separates the nuclear cell, after the conventional method withdraws cell total RNA counter to copy is individually cDNA, the LRP1B specificity directs thing PCR to expand increases the cDNA first chain, after and will expand the production increase purification to measure the foreword. Gathers 10 examples normal people marrow specimen to take normal comparison. Results: the LRP1B gene has the expression in the acute leukemia, in 36 examples samples 16 examples have PCR to expand the production increase, the product afterthe purification measured the foreword the discovery has not had apparent subchange and so on flaw and repetition. 8 examples have in 10 examples normal comparisons expand the production increase. Case of illness group and comparison compare the LRP1B gene expression rate and the relative expression level obviously reduces (p value 0.05). Conclusion: LRP1B to take a candidate tumor suppression gene, plays the vital role in the many kinds of tumors, but in leukemia expression situation still loss of information. This experiment confirmed this gene has the expression in the systema sanguineum, but has not discovered this situation occurrence and so on gene common apparent subflaw and repetition in the leukemia. But confirmed this gene expression level normal comparison obviously decreases in the leukemia, prompts this gene and acute leukemia is taken bad the correlation. For thoroughly studied this gene the function which displayed in the leukemia to lay the foundation. Thus has provided the partial laboratories basis for the tumor gene diagnosis and the treatment.