目的:利用生物信息学方法分析PRKDC在肝癌中的表达及其与肿瘤微环境中免疫细胞浸润、预后的关系.方法:采用Oncomine、UALCAN、HCCDB、HPA、TCGA、Kaplan-Meier、cBioPortal、LinkedOmics、TIMER数据库和R软件包ggstat-splot、IOBR、psych(version 2.1.6)研究PRKDC基因在肝癌组织和正常肝组织中的表达差异,分析基因表达、基因突变与肝癌预后的相关性,并对GO、KEGG通路进行GESA富集分析;分析PRKDC表达与免疫检查点、免疫标志基因、肿瘤免疫细胞浸润、肿瘤微环境中免疫细胞和基质细胞浸润的相关性.结果:肝癌组织PRKDC呈显著高表达,高表达的PRKDC显著缩短肝癌患者OS和DSS,PRKDC基因组改变与肝癌患者OS和DFS无显著相关性;PRKDC表达与B细胞、嗜中性粒细胞、巨噬细胞、CD4+T细胞和树突状细胞呈显著正相关,与多数免疫检查点、免疫标志基因显著相关,与肝癌肿瘤微环境呈显著负相关.结论:PRKDC可作为肝癌诊断、预后和免疫细胞浸润水平的标志物,与免疫检查点、免疫标志基因、免疫细胞浸润性、肿瘤微环境免疫细胞和基质细胞浸润等相关.

目的:探讨开放性零缺血结合同步切缝的肾部分切除术治疗复杂性孤立肾肾癌的可行性、安全性及优势.方法:回顾性分析2017年10月至2021年10月5例于哈尔滨医科大学附属肿瘤医院收治的解剖性和功能性孤立肾肾癌患者的临床资料,均采用开放性零缺血肾部分切除术和术中同步切缝技术,手术由同一术者完成,分析手术时间、术中失血量、术中及术后并发症、术后肿瘤学结果及肾功能指标的变化.结果:5例患者均成功采用开放性零缺血结合同步切缝的肾部分切除术,无中转肾动脉阻断和行挽救性肾切除术.手术时间为135～180 min,术中失血量为300～700 mL,平均失血量为450 mL.5例中1例肾门部肾癌患者术后第1天开始出现尿漏,当日膀胱镜下于输尿管内逆行留置F6双"J"管后,术后第2天尿漏消失.术后随访3～48个月,平均随访时间30.4个月,局部未见肿瘤复发,1例出现肋骨转移.与术前相比,术后各项肾功能指标无明显变化(P＞0.05).结论:开放性零缺血结合同步切缝的肾部分切除术在治疗复杂性孤立肾肾癌安全可行,特别适合复杂和肿瘤体积较大的肾癌,在保护肾脏功能和肿瘤治疗方面有着独特的优势.

Objective:To investigate the effects of gemcitabine on the proliferation, cell cycle, tumor invasion and tumor stem cell characteristics of bladder cancer stem cell-like cells.Methods:Bladder cancer stem cell-like cell lines were selected by using the chemotherapy drug cisplatin combined with serum-free medium technology, CCK-8 method was used to detect the inhibitory effect of different concentrations of gemcitabine on bladder cancer stem cell-like cells, The cell cycle distribution of bladder cancer stem cell-like cells was detected by flow cytometry, qRT-PCR and Western blot were used to detect the expression of ctamer binds transcription factor 4(OCT-4) and human balanced nucleoside transporter 1(hENT-1) in the experimental and control groups.Results:After treatment with 1.6 μmol/L gemcitabine for 24 h, the cell inhibition rate was the highest [(75.2±4.5) %, P<0.001]. Compared with each group, the number of cells in S phase in group C decreased significantly, while the expression of hENT-1 protein was significantly increased ( P<0.001). The tumor invasion ability of bladder cancer stem cell-like cells treated with gemcitabine was decreased ( P<0.001). Conclusions:Gemcitabine has a killing and inhibitory effect on bladder cancer stem cell-like cells, which can weaken the stem cell characteristics and tumor invasion ability.

目的:探讨髂嵴上纵切口在后腹腔镜泌尿外科手术中的应用优势及推广.方法:选择2015年5月-2017年10月在我院采用三通道后腹腔镜途径治疗的肾上腺肿瘤及肾肿瘤患者159例,其中采用传统切口组72例,改良切口组87例,比较两组患者在取标本时间、切口长度(切口长度之和)、切口疼痛评分、切口并发症、下床活动时间、切口疝的发生率和切口复发率等方面的差异.结果:与传统切口组相比,在改良切口组采用肾上腺肿瘤切除术、肾部分切除术和肾癌根治术的病例具有更低的疼痛评分(P＜0.05),其中在接受肾上腺肿瘤切除术和肾部分切除术的患者显示有更短的取标本时间、切口长度和更早的下床活动时间(P＜0.05).改良切口组的患者无切口麻木及膨出,并且由于切口较低,位于腰带的后方及下方,仅能看到不明显的两工作套管的切口瘢痕.结论:后腹腔镜手术采用髂嵴上纵切口既可以用于进观察镜也可用于取标本,具有减轻疼痛、降低切口长度、加快术后活动时间和切口隐蔽的优点,值得广泛开展.

目的 研究CIT基因在前列腺癌中的表达特点及其对PC-3细胞生物学行为的调控作用.方法 采用回顾性研究,收集哈尔滨医科大学附属肿瘤医院收治的106例前列腺癌患者的临床资料,将前列腺癌旁正常组织作为对照,检测患者CIT基因表达量.根据前列腺癌患者CIT基因表达量中位数,分为低表达组(53例)与高表达组(53例),比较两组患者临床病理特征的差异以探讨CIT基因表达量与患者临床病理特征的关系.对人前列腺癌PC-3细胞进行培养,根据siRNA技术干扰CIT基因的表达,分为正常对照组(细胞未进行任何转染)、阴性对照组(转染非特异性对照序列siRNA)和CIT-siRNA组(转染CIT-siRNA).通过CCK-8实验检测CIT基因对三组PC-3细胞增殖能力的影响,并采用划痕实验检测细胞迁移能力,通过Transwell小室实验检测细胞侵袭能力.结果 相比前列腺癌旁正常组织,CIT基因高表达于前列腺癌组织(P<0.01).低表达组与高表达组Gleason评分、N分期、M分期和前列腺特异抗原水平的比较,均存在显著差异(P<0.05);而两组在年龄、T分期和精囊侵袭上的比较,均无显著差异(P>0.05).在siRNAs细胞转染48 h后,阴性对照组和CIT-siRNA组PC-3细胞中CIT蛋白和mRNA表达量较正常对照组均明显下调(P<0.01).CCK-8实验结果 显示,siRNAs转染PC-3细胞2、3 d后,阴性对照组和CIT-siRNA组细胞增殖能力较正常对照组均明显下降(P<0.01).划痕实验和Transwell实验结果 发现,CIT特异性siRNAs转染细胞48 h后,阴性对照组和CIT-siRNA组PC-3细胞迁移距离较正常对照组均明显缩短,且侵袭细胞数量较正常对照组均明显减少(P<0.01).结论 CIT基因在前列腺癌组织中具有高表达的特点,通过沉默该基因表达,可有效干扰PC-3细胞生物学行为,提示CIT基因可能是前列腺癌的一种重要致病基因.

目的 探讨DNA依赖蛋白激酶亚基(DNA-PKcs)基因沉默对肝癌耐药细胞BEL7402/5-Fu细胞凋亡的影响.方法 实验分为空白对照组,siRNA-NC组,脂质体对照组和siRNA-DNA-PKcs实验组;Hoechst33342染色法观察细胞形态学变化;Western blot检测DNA-PKcs,B细胞淋巴瘤-2相关x蛋白(Bcl-2 associated X protein,Bax),凋亡诱导因子(apoptosis inducing factor,AIF),天冬氨酸蛋白水解酶3(cysteine aspartic acid specific protease-3,caspase-3)蛋白水平;免疫荧光检测细胞色素C(Cytochrome c,Cyt-c)释放情况.结果 Hoechst 33342染色检测结果显示siRNA-DNA-PKcs组细胞体积缩小,细胞核边集,呈亮蓝色;Western blot检测结果显示siRNA-DNA-PKcs实验组DNA-PKcs蛋白下调(P＜0.05),Bax、caspase-3和AIF蛋白上调(P＜0.05);免疫荧光结果显示siRNA-DNA-PKcs实验组细胞色素C呈弥散分布于细胞质中,细胞色素C、线粒体平均光密度减少,与对照组比较,差异有统计学意义(P＜0.01).结论 沉默DNA-PKcs表达后可能通过线粒体凋亡途径促进肝癌耐药细胞BEL7402/5-Fu凋亡.

Objective To assess the efficacy and side effects of intravesical instillation of BCG after transurethral resection of the bladder tumor (TURBT) in non-muscle invasive bladder cancer (NMIBC) patients.Methods The clinical data of patients treated with BCG 120 mg per course induced perfusion or more after TURBT from December 2013 to October 2016 in 18 hospitals of northeast China region,were analyzed retrospectively.The first part,data of 106 patients with moderate,high-risk NMIBC were collected.A total of 83 patients were male,while the other 23 patients were female.The average age was 66.7 years old.The clinical staging were T1 in 86(81.1％) cases,Ta in 20(18.9％) cases and carcinoma in situ in 6 (5.7％) patients.Intravesical instillation of BCG was executed after transurethral resection of the bladder tumor.The incidence rate of recurrence and progression during more than 6 months' follow-up time were observed.Multivariate analyses were done by using logistic analysis and Cox proportional hazards regression model with Kaplan-Meier method.The second part,treatment compliance of 276 patients with bladder cancer,including moderate/high-risk NMIBC in 263 cases,moderate/high-risk NMIBC followed with renal pelvis/ureteral carcinoma in 8 cases were and moderate/high-risk NMIBC with renal pelvis/ureteral carcinoma in 5 cases who treated with BCG after the surgeries,were observed.Patients consisted of 211 males and 65 females with average age of 68.3 years.Results With a median follow-up of 12 months,9 (8.5％) patients experienced tumor recurrence and 2 (1.9％) patients were found progression in the first part.The one-year cancer free recurrence rate of the patients was 91.5％.Statistically significant prognostic factors for recurrence identified by multivariable analyses were prior recurrence of the tumors (OR =3.214,95％CI0.804-12.845,P =0.099).In the second port,an incidence rate of adverse effects was 64.1％ (177/276).The Ⅲ/Ⅳ degree complications were occurred in 11 patients and satisfactory outcomes achieved with active treatment.A total of 36 patients withdrawal with the major causes were recurrence and progression of bladder tumor in 12 cases (4.4 ％),9 cases (3.3 ％) with economic reasons and 11 cases (4.0％) with serious complications.Conclusions NMIBC patients treated with intravesical BCG therapy have approving cancer free recurrence rates and acceptable adverse effects.Prior recurrence may be prognostic factor of recurrence after intravesical BCG therapy.

为了了解遵义市犬钩端螺旋体病流行情况以及人群对钩端螺旋体病的预防意识,采用犬钩端螺旋体抗体ELISA试剂盒对遵义市320份犬血清样品进行钩端螺旋体血清流行病学调查,并通过网络问卷调查对遵义市646名大学生开展有关钩端螺旋体病的社会调查.血清学试验结果显示,犬钩端螺旋体的总阳性率为5.00％(16/320),犬钩端螺旋体病感染在来源、年龄与性别上差异无显著统计学意义(P>0.05);问卷调查结果中33.13％(214/646)的受访者认为钩端螺旋体病能通过动物传染给人,有45.67％(295/646)的受访者曾经被犬抓伤或者咬伤过,56.35％(364/646)的受访者认为被犬咬伤或抓伤后应立即采用肥皂水或清水彻底冲洗伤口.研究表明遵义市犬钩端螺旋体阳性率较高,并且人群对钩端螺旋体病防范意识不足,存在较大的流行风险.

目的 探讨重组质粒pαACP-HSP70对半乳糖诱导大鼠晶状体组织Smac mRNA水平和蛋白水平影响.方法 将大鼠晶状体组织培养6h后,选取透明27只晶状体组织随机分为空白对照组、半乳糖组、半乳糖+重组质粒pαACP-HSP70组(简称α-70组),每组9只;用阳离子脂质体转染法将重组质粒pαACP-HSP70质粒导入α-70组;荧光显微镜下观察重组质粒pαACP-HSP70在晶状体组织的表达情况;Real-time PCR检测各组HSP70、Smac的mRNA水平;Western blot检测各组HSP70、Smac蛋白表达.结果 α-70组转染重组质粒pαACP-HSP70后,与空白对照组、半乳糖组相比,晶状体有绿色荧光蛋白表达,HSP70 mRNA、蛋白水平上调,Smac的mRNA、蛋白水平下调,差异有统计学意义(P＜0.01).结论 重组质粒pαACP-HSP70成功转入晶状体组织并上调HSP70基因表达,抑制Smac基因表达.

目的 识别引起肾透明细胞癌(renal clear cell carcinoma,ccRCC)发病的关键基因和其生物学过程.方法 分别从GEO和TCGA数据库中下载相关数据,应用R软件中的limma和EdgeR包筛选出在癌症样本和正样本中差异表达的基因(differentially expressed genes,DEGs).然后,利用STRING数据库构建蛋白质互作(protein-protein interaction,PPI)网络,并基于网络进行模块挖掘和功能注释.结果 共筛选出4 667个DEGs,构建了包含519个DEGs(节点),2 394对互作(边)的PPI网络.挖掘到的得分最高的三个模块注释到了G蛋白偶联受体信号通路、信号转导、防御反应、炎症应答、细胞迁移调控、细胞增殖与分化和神经活性配体-受体相互作用等在ccRCC的发展中有重要作用的生物学过程中.识别出与ccRCC发生发展密切相关的关键基因CA9、CDKN2A、MET、HLA-C、VEGFA、LDHA和TERT等.结论 本研究筛选出的几个关键基因和功能通路可能在ccRCC的发展中起关键作用.

我国的医疗水平已经跻身于世界前列,能有效地治疗多种病症.但仍然有许多疾病还需要不断的完善治疗方法.肿瘤就是其中的一种.经过长期对肿瘤疾病的治疗,肿瘤这种疾病已经产生了耐药性,其耐药性有原发耐药性(PDR)和多药耐药性(MDR),对于原发耐药性时在通过诱导药物治疗时产生的耐药性,且只对诱导药物起作用.而多药耐药性是肿瘤病原体在对其中一种治疗药物产生耐药性后,对其他的治疗药物产生交叉耐药性,值得关注的是,其他的药物在结构与治疗方面都各不相同,但是均产生了耐药性,这也是肿瘤治疗困难的原因之一,因此本文就肿瘤耐药机制研究进行探讨,望其对肿瘤的治疗提供有效帮助.

Objective In this study,we seek to investigate whether Artemis is involved in multidrug resistance of the human hepatocellular carcinoma cell line,namely BEL-7402/5-FU cells,after knockdown of Artemis.Methods We transfected pSGU6/GFP/Neo/shArtemis interference plasmids to BEL-7402/5-FU cells (shArtemis group) and examined Artemis and P-gp mRNA by Real-time PCR.We carried out MTT assay to calculate the IC50values and RI of shArtemis group induced by antitumor agents 5-fluorouracil,MMC and sorafenib for 48 h,respectively.We performed Western-blot to observe the expression levels of p-Artemis and ATM protein.We detected γ-H2AX and Artemis proteins changes after treatment of inhibitors of ATM,KU55933,and MMC in BEL-7402/5-FU by Western-blot.Results We observed that shArtemis group had markedly decreased expression levels of Artemis and P-gp mRNA (P ＜0.05),but also significantly reduced the IC50values and RI of shArtemis group following treatment with 5-fluorouracil,MMC and sorafenib (P ＜ 0.05).Subsequently,the results of the Western-blot indicated that p-Artemis and ATM proteins expressions were decreased in shArtemis group (P ＜ 0.05).In this context,further treatment with KU55933 of BEL-7402/5-FU cells exposed to MMC resulted in a lower expression ATM (P ＜ 0.05) and an increased expression γ-H2AX (P ＜0.05),however,Artemis protein level was not astatistically significant one (P ＞ 0.05).Conclusion Our analysis suggested that the targeted inhibition of Artemis significantly enhanced chemo sensitivity of BEL-7402/5-FU cells,and this effect appears to be associated with the function of Artemis acting as a molecular switch involved in DNA damage repair by regulating ATM.

目的 研究DNA依赖蛋白激酶催化亚基(DNA-PKcs)基因沉默后对肝癌耐药细胞Bel7402/5-Fu细胞凋亡的影响.方法 实验分为空白对照组、脂质体对照组、NC对照组、siDNA-PKcs实验组.采用噻唑蓝(MTT)法检测耐药细胞Bel7402/5-Fu的耐药性;采用阳离子脂质体法将siDNA-PKcs寡核苷酸片段转染Bel7402/5-Fu细胞;Real-time PCR和Western blot分别检测细胞DNA-PKcs mRNA及蛋白的表达情况;倒置荧光显微镜下观察细胞形态变化(Hoechst33342染色法);流式细胞术检测细胞凋亡情况(AnnexinV/PI双染法);Western blot检测B细胞淋巴瘤-2相关x蛋白(Bax)、B细胞淋巴瘤/白血病-x基因长片段(Bcl-xl)蛋白的表达情况.结果 MTT检测Bel7402/5-Fu细胞的半抑制浓度(IC50)明显增高,耐药细胞Bel7402/5-Fu的耐药指旨数为13.13;DNA-PKcs的mRNA与蛋白表达水平明显减少,设计的siDNA-PKcs特异序列能有效沉默细胞DNA-PKcs的表达;Hoechst-33342染色检测结果显示siDNA-PKcs实验组细胞体积缩小、细胞核边集、呈亮蓝色;流式细胞术检测结果显示,siDNA-PKcs实验组细胞凋亡率比其余组增加(P＜0.01);Western blot检测结果显示siDNA-PKcs实验组Bax蛋白表达水平比其余组高,Bcl-xl蛋白表达水平比其余组低(P＜0.01).结论 siDNA-PKcs能诱导肝癌耐药细胞Bel7402/5-Fu细胞凋亡,并能上调促凋亡蛋白Bax的蛋白表达水平,抑制抗凋亡蛋白Bcl-xl的蛋白表达水平.

医学院校实验室队伍的建设、改革与发展,面临着诸多问题,而实验技术队伍的综合素质培养与提高是亟需解决的问题之一.由于历史原因,高校实验室队人员年龄偏高,学历偏低,创新能力不足,接受新知识新技术能力偏低等,造成实验室队伍建设的落后现状制约高校教学,科研和服务社会水平的发展.针对上述问题,对实验技术人员现状及实验技术人员综合素质的培养进行探讨.

Objective To investigate the inhibitory effect of total saponins from the fruit of Stauntonia chinensis DC on human lung cancer cell (A549),human hepatocellular cell (BEL-7402) and human gastric cancer cell (SGC-7901) in vitro.Methods The A549 cells,BEL-7402 cells and SGC-7901 cells were randomized into seven groups: negative control group,total saponins (40,80,160,320,640 mg·L-1) groups and mitomycin (10 mg·L-1) group.The proliferation inhibition rate in each group was calculated by MTT after incubated with drugs for 12,24,48 h.Results The total saponins from the fruit of Stauntonia chinensis DC could significantly inhibit the proliferation of A549 cells,BEL-7402 cells and SGC-7901 cells were in dose-and time-dependent.A549 cells were incubated for 12,24,48 h by total saponins,IC50 were 492.38,293.43,141.84 mg·L-1 respectively.BEL-7402 cells were incubated for 12,24,48 h by total saponins,IC50 were 427.74,243.91,101.79 mg·L-1 respectively.SGC-7901 cells were incubated for 12,24,48 h by total saponins,IC50 were 463.35,256.77,113.22 mg·L-1 respectively.Conclusions The total saponins from the fruit of Stauntonia chinensis DC could obviously inhibit the proliferation of A549 cells,BEL-7402 cells,and SGC-7901 cells.Among them,BEL-7402 cells were the most sensitive to the total saponins.

Objective To investigate the reverse effect of total saponins from the fruit of Stauntonvine chinensis DC on the multidrug resistance (MDR) cell BEL-7402/5 FU, and explore the related mechanism.Methods The cells toxicant effect of 5-fluorouracil (5-FU), adriamycin (ADM), mitomycin (MMC) and total saponins from the fruit of Stauntonvine chinensis DC to BEL-7402 cells and BEL-7402/5 FU cells, and the reversal effect of total saponins from the fruit of Stauntonvine chinensis DC were evaluated by MTT assays.The expression of P-glycoprotein (P-gp) was detected by western blotting (WB).Results The resistance index of BEL-7402/5 FU cells to 5-FU, ADM, MMC were 21.71, 2.73 and 2.11 respectively.Total saponins from the fruit of Stauntonvine chinensis DC could significantly inhibit the proliferation of BEL-7402 and BEL-7402/5 FU cells.The available reversal concentration of total saponins from the fruit of Stauntonvine chinensis DC were 5 mg/mL and 10 mg/mL, and the reversal index were 1.36 and 1.93 respectively.After treated with total saponins from the fruit of Stauntonvine chinensis DC and 5-FU, the expression of P-gp was decreased.Conclusion The total saponins from the fruit of Stauntonvine chinensis DC could partly reverse the MDR of BEL-7402/5 FU cells, the reversal mechanisms may be due to down regulating the expression of P-gp.

Objective The aim of this study is to synthesize the chitosan-sulfobetaine (CS-DMAAPS) copolymer as siRNA delivery vector,and investigate the transfection efficiency on HepG-2 cells.Methods The sulfonic acid betaine (DMAAPS) with C=C was grafted onto chitosan (CS) by Michael addition method,and the structure of the copolymer was characterized with 1H-NMR.The cytocompatibility of CS-DMAAPS was examined through CCK-8 method.The transfection efficiency of CS-DMAAPS was observed with fluorescence microscopy.And the silencing efficiency of Bcl-2 gene expression was detected after siRNA gene transfection.Results NMR data showed that DMAAPS had been successfully connected to CS.The transfection efficiencies of the copolymers with different CS-DMAAPS/siRNA mass ratio (m0/mt:32,16,8,4 and 2) were not same.They were 33.78%,45.82%,50.98%,69.89% and 81.22%,respectively.Real-time PCR results showed that the silencing efficiency of Bcl-2 gene expression in HepG-2 cells was 26.8% after transfection by using nanoparticles (m0/mt:4).Conclusion CS-DMAAPS could be a feasible new gene delivery material with good biocompatibility,which could delivery siRNA into HepG-2 to inhibit the expression of Bcl-2 gene.

目的:随着人们生活节奏的加快和生活环境的变化,各种肿瘤的发病率越来越高,给人们的健康和生活造成严重的影响,危及人类的生命安全.其中肝癌是最为常见的恶性肿瘤之一.本文简要介绍肿瘤血管生成与肝癌的研究进展,为临床提供参考.

目的 探讨DNA依赖蛋白激酶亚基(DNA-PKcs)基因沉默后对肝癌耐药细胞Bel-7402/5-FuDNA损伤修复功能的影响.方法 采用阳离子脂质体法将shDNA-PKcs干扰质粒转染Bel-7402/5-Fu细胞,根据荧光细胞数计算转染率,实时荧光定量聚合酶链反应(qRT-PCR)及Western blot检测DNA-PKcs的沉默效率;Western blot检测TopoⅡ、γ-H2AX蛋白表达;5-乙炔基-2'脱氧尿嘧啶核苷法检测细胞DNA合成.结果 质粒的转染效率为67％;qRT-PCR及Western blot检测结果显示,DNA-PKcs mRNA及蛋白水平的沉默效率分别为82.6％和71.8％;Western blot结果显示,实验组TopoⅡ蛋白表达水平比对照组低,差异有统计学意义(P＜0.05),γ-H2AX蛋白表达水平比对照组高,差异有统计学意义(P＜0.01).5-乙炔基-2'脱氧尿嘧啶核苷法结果显示,实验组DNA合成率比对照组低,差异有统计学意义(P＜0.05).结论 shDNA-PKcs干扰质粒能下调Bel-7402/5-Fu细胞中DNA-PKcs、TopoⅡ蛋白的表达,抑制Bel-7402/5-Fu细胞DNA合成.

目的探讨转染sh Artemis干扰质粒对人肝癌细胞BEL-7402/5FU DNA损伤的影响。方法将人肝癌细胞分为正常对照组、脂质体对照组、空质粒对照组和转染sh Artemis干扰质粒实验组（sh Artemis实验组）;建立DNA损伤模型,分别用0.625、1.25、2.5、5.0和10.0μg/m L的丝裂霉素C作用BEL-7402/5FU细胞24和48 h,MTT法检测细胞活性,Western blot观察磷酸化组蛋白H 2 AX（γ-H 2 AX）的表达;转染Artemis干扰质粒,检测Artemis、γ-H 2 AX表达;彗星实验检测DNA的损伤。结果 0.625、1.25、2.5、5.0和10.0μg/m L的丝裂霉素C作用48h后肝癌细胞活力明显下降（P＜0.05）;丝裂霉素C刺激细胞48 h后,γ-H 2 AX的表达量随着药物浓度的增加而增加;转染sh Artemis干扰质粒后,γ-H 2 AX的表达量较正常对照组增加（P＜0.05）;sh Artemis实验组较正常对照组拖尾DNA量增加（P＜0.05）。结论丝裂霉素C能够诱导肝癌细胞损伤;转染sh Artemis干扰质粒促进丝裂霉素C诱导的人肝癌细胞BEL-7402/5FU DNA损伤。