目的 探讨地诺孕素联合散结镇痛胶囊对子宫内膜异位症的临床疗效及各血清学指标的影响.方法 选取2017年10月至2019年10月空军军医大学第一附属医院妇产科收治的94例子宫内膜异位症患者为研究对象,将受试者根据随机数字表法分为两组,对照组和研究组每组各47例,对照组患者口服散结镇痛胶囊治疗,研究组患者在对照组的基础上加用地诺孕素治疗,比较两组的治疗总有效率、治疗前后各性激素指标与血清因子水平变化及用药安全性.结果 研究组患者治疗总有效45例(占95.74％),对照组治疗总有效37例(占78.72％),两组比较差异有统计学意义(χ2=6.144,P<0.05).治疗前后两组患者黄体生成素(LH)、卵泡刺激素(FSH)水平比较差异均无统计学意义(P>0.05),治疗后两组患者雌二醇(E2)、孕酮(P)水平较治疗前均明显降低(研究组:t值分别为32.475、25.386,P<0.05;对照组:t值分别为20.473、15.483,P<0.05),且研究组E2、P水平显著低于对照组(t值分别为13.273、9.658,P<0.05).治疗前两组患者血清CA125、基质金属蛋白酶(MMP)-2、MMP-9、血管内皮生长因子(VEGF)、半乳糖凝集素-3(Gal-3)水平差异均无统计学意义(P>0.05);治疗后两组患者血清CA125、MMP-2、MMP-9、Gal-3及VEGF水平均显著低于治疗前(t=13.267～52.493,P<0.05),且研究组患者血清CA125、MMP-2、MMP-9、Gal-3及VEGF水平均显著低于对照组(t值分别为12.473、14.358、21.356、9.332、20.342,P<0.05).两组患者药物引起的不良反应发生率比较差异无统计学意义(P>0.05).结论 地诺孕素联合散结镇痛胶囊可有效降低各性激素与血清因子水平,疗效安全显著,对促进子宫内膜异位症患者病情康复具有积极意义.

Aim To investigate the role of captopril in insulin resistance of endothelial cells induced by high glucose.Methods 1 .Improvement effect of captopril on insulin resistance in HUVECs was observed.The HUVECs were seeded in a 6-well plate and were ran-domly divided into 5 groups,namely,control group, IR group,IR together with different Cap concentrations (low,medium and high concentration),respectively. 2.Improvement effect of Cap on insulin resistance was mediated by PPARγin HUVECs.HUVECs were ran-domly divided into 6 groups,namely,control group, control +PPARγinhibitor (PI)(1 .0 μmol · L -1 ) group,IR group,IR +PI(1 .0 μmol·L -1 )group,IR +Cap(1 ×1 0 -5 mol·L -1 ) group,and IR +Cap +PI (1 .0 μmol·L -1 )group.All indicators were detected. Results After HUVECs were incubated with media containing 33 mmol·L -1 of glucose for 48 h,the NO levels were significantly decreased while ET-1 levels were significantly elevated,showing a significant differ-ence between IR group and control group (P <0.01 ). The expression levels of PPARγmRNA and its protein were somewhat up-regulated,but there was no signifi-cant difference between IR group and control group (P>0.05).When the HUVECs in IR group were treated with DMEM containing glucose (33 mmol·L -1 )for 48 h and insulin for 30 min,the expression levels of PPARγmRNA and its protein in Cap groups were simi-lar to those in the IR group,and there was no signifi-cant difference between the two groups (P >0.05 );however, the expression levels of phosphorylated PPARγprotein in Cap groups were increased compared with IR group (P <0.05).The levels of NO were sig-nificantly increased whereas the levels of ET-1 were decreased in Cap groups,which had significant differ-ences compared with IR group (P <0.05).Nonethe-less,pre-treating with GW9662,a PPARγinhibitor, the improvement effects of Cap were markedly abol-ished.Conclusions Captopril could improve high glucose-induced insulin resistance of endothelial cells mediated by PPARγ,and the underlying mechanisms are related to the activation of PPARγ,rather than its expression.

Aim To construct eukaryotic expressing plasmids of short hairpin RNA(shRNA) targeting the PPARγ gene and to evaluate their inhibitory effect on PPARγ expression in human umbilical veinendothelial cells(HUVECs).Methods Three pairs of complementary shRNA oligonucleotides targeting the PPARγ gene were designed,synthesized,annealed and inserted into the pGPU6/GFP/Neo plasmid.The recombinant plasmids were identified by restriction enzyme analysis and sequence analysis.The inhibitory effect of recombinant plasmids on PPARγ expression in HUVECs was detected by Western blot.Results After restriction enzyme analysis and sequence analysis,three eukaryotic expression plasmids of shRNA targeting the PPARγ gene were successfully constructed.Western blot analysis showed that pGPU6/GFP/NeoshRNAPPARγ3 reduced PPARγ expression by 63.2% in HUVECs induced with high glucose.Conclusion Three eukaryotic expression plasmids of shRNA targeting the PPARγ gene are successfully constructed.These recombinant plasmids can efficiently inhibit PPARγ expression in HUVECs.

A CNC tube cutting system's structure and its working principle in the glass tube production line are introduced.CNC system is designd from the control cycle and trombone cutter rotation speed of the relationship between the start and achieve the purpose of ensuring the length of glass tubes.Solved a larger problem caused by that its rate cutting process can not adapt to changes in trombone which leads to the larger length error of the glass tubes in the glass tubes' production process.

OBJECTIVE To design a new route to prepare sulforaphane.METHODS The target compound was synthesized from DMSO by three steps.RESULTS and CONCLUSION The structure of target compound was confirmed by IR,1HNMR,MS and the total yield was 51.1%.This procedure has advantages of simple starting material and convenient operations.

Objective: To design a new route to prepare 1-chloro-4-methylsulfinyl- butane. Methods: The target compound was synthesized successfully from DMSO by two steps. Results and Conclusion: The structure of target compound was confirmed by IR, 1HNMR and the total yield was 65. 6%. This procedure has advantages of simple starting material and convenient operations.

N-Carbobenzoxy-L-homoserinelactone(Ⅲ) was prepared via N-alkylation reaction of L-methionine(Ⅰ) and iodoacetamide(added a little iodine) with benzyl chloroformate as amino protecting agent.When Na_2CO_3-NaHCO_3 was used as the alkali,the mixture reacted at 30℃ for 22 h to give N-carbobenzoxy-L-methionine(Ⅱ).When n(Ⅱ)∶n(iodoacetamide)=1∶1 and adding a little iodine,the mixture was reacted at 50℃ for 72 h and then refluxed at 105℃ for 4 h to give Ⅲ with a total yield of 73.9% and purity of 98.1%.The structure was confirmed by IR and()~1H NMR.

L-Canaline,an O-substituted hydroxylamino anticancer agent,was prepared from N-Cbz-L-homoseine lactone by carboxylic protection,bromination,condensation and then hydrolysis with an overall yield of about 62%.

D-Pro·R-TA salt was obtained through the reaction of L-Pro with R-TA in n-Butyric acid with butyl aldehyde as catalyst.The influences of temperature,time,carboxylic acid dosage and catalyst dosage on the yield and optical purity were discussed.The optimal process conditions are as follows: reaction temperature 90℃,reaction time 6 h,n-Butyric acid as solvent(60 mL) and L-Pro∶R-TA∶butyl aldehyde = 1∶1∶0.1(mol).Treatment of the obtained salt D-Pro·R-TA with 35%ammonia in methanol gare D-Pro is obtained with the yield of 91.74% and the optical purity of 98.7% based on the starting L-Pro.

L-canavanine, the analog of L-arginine ,is a natural non-protein amino acid. It is incorporated in place of L-arginine into newly synthesized protein in a wide variety of organisms, resulting in the formation of non-functional proteins. So it is an effective anticancer agents, particularly for pancreatic cancer. The mechanisms of anticancer and the methods for preparation L-canavanine are specifically described.