A linear mixing model of the two-component mixed edible oil was proposed based on the assumption that the absorption coefficient of the two-component mixed edible oil can be obtained by the linear combination of absorption coefficients of two raw oils according to the mixing proportion. Based on the Lambert-Beer's Law, a series of absorption equations of the incident light was constructed to describe variations of light intensity after the same source light respectively passing through the two raw oils and their proportional mixture with the same thickness. As a result, a formula was derived to calculate the mixing proportion of the two-component mixed edible oil, which was a new method to quantitatively analyze the proportion of adulterated edible oil. According to the theory of errors, the calculation difference of mixing proportion to the actual value was analyzed by the total differential methods, and the result demonstrated that there was an optimal wavelength where the products of the absorption light intensity of two raw oils and the difference value between their absorbance were larger, and less calculation difference could be obtained. A VIS/NIR spectral detection system was assembled and used to validate the mixing proportions of corn oil adulterated in peanut oil, soybean oil adulterated in peanut oil and soybean oil adulterated in corn oil. The results showed that the principle of choosing the optimal testing wavelength was correct, and the relative errors between calculation values and actual values were less than 5% for adulterating proportions above 10% (v/v), as well as the correlation coefficients were 0.999 4, 0.999 7 and 0.999 3 with RMS errors of 0.006 9, 0.005 1 and 0.007 6. The results proved that the proposed linear mixing model was practicable. The comparative experiments, which were operated on the stacked series of two separate oil samples with same mixing proportions, also verified the correctness of the linear mixing model and the method for choosing the testing wavelength. In addition, these comparative experiments revealed that the parallelism of light source and verticality of oil vessels were important to the detection accuracy of mixing proportion. This paper introduces a new method of detecting the mixing proportion of two-component mixed edible oil which differs from the traditional method of spectroscopy combined with chemometrics. In this method, the mixing proportion of two-component mixed edible oil can be precisely computed only after three times data collection of absorption spectra of the two raw oils and their mixture.

Objective To theoretically study the non-invasive measurement method of blood hemoglobin in vivo based on the time-resolved approach combined with occlusion spectroscopy.Methods The dynamic dual wavelength time-resolved transmission was analyzed based on the artificial blood flow kinetics condition on a human finger model.The sensitivity of hemoglobin measurement was improved by Laplace transforming of time-domain data.The method was validated using hemoglobin with a mass concentration range of 6～16 g/dl.Results The simulation results showed that compared with the continuous wave method,the time-resolved method could provide higher detection sensitivity using Laplace transform parameter p =5 × 1010 s-1.Conclusions The sensitivity of hemoglobin measurement can be enhanced when early arriving photons are emphasized by Laplace transformingthe time-resolved transmission with a small positive parameter.

研制了一种808 nm/635 nm双通道半导体激光器驱动电源,主要由恒流源驱动和温控电路两部分组成.通过12位DA的输出电压对两个通道的驱动电流进行控制,808 nm和635 nm通道的电流驱动范围分别为0～3A和0～1A,控制精度分别为0.73 mA和0.24 mA.温控电路由温度传感器、差分放大电路、比例积分微分(PID)控制电路和半导体制冷器(TEC)驱动电路组成,采集的温度信号与设定的温度值进行差分放大后通过硬件PID控制驱动TEC进行制冷制热,实现温度控制以保证输出功率和波长的稳定.在室温23℃下进行应用测试(设定工作温度为25℃),10 min内,808 nm通道在2.2A驱动时,功率不稳定度为1.805％;635 nm通道在640 mA驱动时功率不稳定度为1.233％.两个通道的P/I特性曲线线性拟合结果的校正决定系数(Adj.R-Square)都大于0.998.

搭建红外波段双波长(1940 nm和980 nm)激光诱发痛觉刺激系统,探索基线温度控制、激光吸收导热涂层、致痛区域控制对痛觉刺激效果的影响.利用热电偶测温模块实时监测基线温度控制及涂抹20％氧合血红蛋白溶液时离体猪皮皮肤表面温度的变化;利用滤光片实现致痛区域控制.利用1940 nm激光模块实现了40℃基线温度控制,结合980 nm脉冲激光模块完成了联合痛觉刺激实验;皮肤表面涂抹20％氧合血红蛋白溶液时温降时间(110 ms)远低于空白对照(1.2 s)和风冷散热(341 ms),可提升痛觉信号质量;列举了三种不同尺寸滤光片下的温度场分布情况,证明了致痛区域的可控性.双波长红外激光诱发痛觉系统提出的三种技术对痛觉刺激效果有较好的促进作用,证明了这种痛觉刺激模式的可行性和可信性.

Objective In order to further improve the noninvasive measurement precision of human blood glucose and achieve clinical requirements,experiments were conducted to measure human blood glucose by a novel measurement method our group proposed.Methods The blood flow of the tested parts was stopped by pressure,and dynamic distribution of transmission spectra was measured by time-correlated single-photon counting (TCSPC) technology under the dual wavelength light incident.Oral glucose tolerance test (OGTT) was conducted on three human subjects,and the early arriving photons were selected by Laplace transform.Meanwhile,the human blood glucose concentration was measured using the Roche glucose meter.Results The best curve fitting was got when the Laplace parameter was 1Gs-1 (determining parameter R2=0.0922).Conclusions The experimental results showed that better measurement accuracy can be obtained by selecting appropriate Laplace parameter,and noninvasive measurement of human blood glucose under flow control of time gate was feasible.

Repair of peripheral nerve injury is an important topic in the field of neural tissue rehabilitation research.At present there are many new technologies that can realize the treatment of peripheral nerve injury.As the research on the effect of laser radiation on biological mechanism develops,a new method for tissue regeneration is applied to the field of nerve injury repair.This paper is mainly centered on the recovery mechanism of low-level laser radiation,the function recovery after peripheral nerve injury and nerve tissue scarring inhibition.The new progress of laser treatment and nerve injury repair are reviewed.

研究激光针灸在不同的作用方式和参数设置下,对Ⅱ型胶原蛋白诱导的关节炎(CIA)模型大鼠血清白细胞介素1β(IL-1β)、白细胞介素15(IL-15)、白细胞介素17(IL-17)、肿瘤坏死因子α(TNF-α)、血管内皮细胞生长因子(VEGF)和皮质醇(COR)的影响.将大鼠随机分为模型组,药物组,针刺组,深部激光针灸1 mW组、5 mW组、10 mW组,表面激光针灸50 mW组、100mW组、150mW组,并设正常对照组,针灸穴位选择足三里和肾俞.治疗10 d后取材,采用双抗体夹心酶联免疫吸附试验法(ELISA)测定血清因子水平.模型组的血清IL-1β、IL-15、IL-17、TNF-α和VEGF均较正常组显著升高,有统计学意义(均为P＜0.001),COR较正常组显著降低,有统计学意义(P＜0.001).治疗后,各治疗组的IL-1β、IL-15、IL-17、TNF-α和VEGF均较模型组有不同程度的降低,除个别组外,与模型组的差异均有统计学意义(P＜0.05、P＜0.01、P＜0.001);各治疗组的COR均较模型组显著升高,有统计学意义(P＜0.001).各治疗组间相比,血清IL-1β水平在针刺组中最高,在表面激光针灸50 mW组中最低,二者差异有统计学意义(P＜0.05);血清IL-15水平在针刺组中最高,在药物组中最低,差异无统计学意义(P＞0.05);血清IL-17水平在深部激光针灸1 mW组中最高,在表面激光针灸100 mW组中最低,差异有统计学意义(P＜0.001);血清TNF-α水平在深部激光针灸1 mW组中最高,在药物组中最低,差异有统计学意义(P＜0.001);血清VEGF水平在深部激光针灸50 mW组中最高,在深部激光针灸5 mW组中最低,差异有统计学意义(P＜0.05);血清COR水平在针刺组中最低,在表面激光针灸150 mW组中最高,差异有统计学意义(P＜0.001).激光针灸能够有效降低CIA大鼠血清IL-1β、IL-15、IL-17、TNF-α、VEGF等促炎因子的表达水平,提高血清COR等抗炎因子的表达水平,其作用效果与激光针灸的作用方式和参数设置有关.

A novel near-infrared (NIR) diffuse correlation spectroscopy (DCS) has recently been developed for noninvasive monitoring of tumor blood flow during photodynamic therapy (PDT).DCS offers several attractive new features for tumor blood flow measurement such as noninvasiveness,portability,low cost,high temporal resolution and relatively large penetration depth.DCS technology has been utilized for continuous measurement of tumor blood flow before,during and after PDT in both animals and humans.The ultimate goal is to predict treatment outcomes from the measured tumor hemodynamic responses to PDT.

Objective To study the influence of laser acupuncture on the pathological change of synovial tissue in the Type Ⅱ collagen-induced arthritis (CIA) model rat.Methods The rats were randomly divided into normal group,model group,drug group,50mW laser acupuncture group,100mW laser acupuncture group and 150 mW laser acupuncture group,with the laser spot diameter of 0.3cm.Rats were exposed to the laser for 10 minutes each time,and Zusanli and Shenshu were chosen as the test acupoints.Material was drawn10 days after the intervention,and pathological section of ankle joint was made and stained with HE.The inflammatory cell infiltration,fibrous tissue hyperplasia,synovial cell hyperplasia and macrophage hyperplasia of the synovial tissues were observed.Results The rating score and total score in terms of the synovial cell hyperplasia(P＜0.001),inflammatory cell infiltration (P＜0.01),macrophage hyperplasia (P＜0.01) and fibrous tissue hyperplasia (P＜0.001) of the model group were significantly reduced,compared with the normal group.After treatment,the rating score and total score of treatment group showed different degrees of reduction in the above-mentioned aspects,of which,the score for the fibrous tissue hyperplasia of the 50 mW laser acupuncture group (P＜0.01),the score for the synovial cell hyperplasia (P＜0.05) and fibrous tissue hyperplasia (P＜0.01) of the 100mW laser acupuncture group as well as the total score (P＜0.01) of the 150mW laser acupuncture group were significantly better than that of the model group.In addition,the score for the synovial cell hyperplasia of the100mW laser acupuncture group was significantly better than that of the drug group (P＜0.05).Conclusion The laser acupuncture has significant inhibition effect on the pathological changes such as synovial tissue hyperplasia,fibrosis hyperplasia of CIA rat,and there is certain relationship between the effects and the parameter setting of laser.

在光腔衰荡光谱技术中衰荡信号虽不受入射光强变化的影响,但入射光强大小并非任意选取.通过理论计算结合实验分析,探讨脉冲光腔衰荡光谱技术中激光单脉冲能量的选取,结果显示激光脉冲能量选取受反射镜的反射率、探测器灵敏度、增益及信噪比等因素限制.当入射光强度选取过低,在探测器本身灵敏度和信噪比的限制下,信号被淹没于噪声中,无法检测信号;入射光强度过高,输出的光电流随光强的增大而趋向于饱和,探测器的响应呈现非线性,甚至出现漂白现象或损坏探测器.实验中,激光光源单脉冲能量范围在33 nJ到1μJ较为合适.

Objective To observe the effects of photodynamic therapy (PDT) on proliferation and apoptosis of human breast carcinoma cell line MCF-7 in vitro.Methods MCF-7 cells were treated with PSD-007 for 2 h under the influence of low-level laser (635 nm) therapy at different doses.Then the optical density (OD) values and survival rates of MCF-7 cells were measured by MTT assay at different time (0.5,1,3,6,12,24 h) after PDT.The nucleus morphological changes of the MCF-7 cells stained by DAPI were observed under the reversal fluorescence microscope.The apoptosis was analyzed using flow cytometry through Annexin/PI double-labeled staining.Results PSD-007 mediated PDT inhibited the proliferation of MCF-7 cells,which depended on laser power and time after PDT.The morphological study proved that the multinucleate giant cells existed in the apoptotic cells in PSD-007 PDT.Flow cytometry of Annexin/PI double-labeled staining verified the results as well.Conclusion PDT inhibit the proliferation of MCF-7 cells in vitro by inducing apoptosis,and the results show an effect of time and dose dependence.

In order to provide a reference for clinical applications, effects of Low Intensity Laser Irradiation(LILI) with combination of 635/808nm on cutaneous wound healings are evaluated. Non-surgical treatment of 60 patients with malignant tumors, the test group and control group of 30 cases is randomly selected. The incision site of patients has been irradiated with laser, which generated two lasers. One was red laser with wavelength at 635 ± 5nm and power density at 1~6.5 mW/cm^2; the other was infrared laser with wavelength at 808 ± 5nm and power density at 11~32 mW/cm^2. Laser treatment was started 24 hours after the end of surgery, each Days once every 15 minutes exposure to treatment endpoint. He-Ne laser treatment began 24 hours after the end of surgery, day 1, each exposure 15 minutes, until the end of treatment (hospital or wound healing stitches). The result shows that LILI with the combination of 635/808 nm could improve cutaneous wound healings of patients significantly rather than using He-Ne laser.

<正>血氧饱和度的检测对人体健康和患病组织的血液循环状态的判定极其重要。采用多波长近红外光对人体血氧饱和度进行非接触检测。首先从电路上设计了恒流源和信号滤波放大电路:恒流源电路给激光管提供稳定的电流和相应的开关时间;光电信号采集滤波放大电路有效滤除背景噪声,提高信噪比。其次,在数据信号处理上,采用基于小波变

Objective To study the feasibility of analyzing photosensitizers absorbing process by cells based on image processing technology.Methods Fluorescence images of Laryngeal cells were taken by inverted fluorescence microscope after adding photosensitizers for a certain time.Parameter L that reflects the fluorescence intensity of cells in different times was obtained and the calculation results were compared.Results The fluorescence intensity of cells increased over time.Sobel operater and Otsu algorithm can both reflect the fluorescence intensity of images.Conclusion Image processing technology can effectively analyzes the process of photosensitizers absorbed by cells.

Glaucoma screening should follow the simple,fast and effective principles.A rapid high-precision screening analyzer is developed to meet the needs of large-scale screening.Intensity uniformity about each stimulus point of the analyzer is good.By using fiber conduction technology,intensity difference between the stimulus points of screen is less than 5%.Moreover,the stimulation point intensity has high precision.By using single chip pulse width modulation,the intensity accuracy reaches 0.001 Nit.Combined with four-point theory for rapid screening,the early diagnosis of glaucoma has been completed.

For big noises tire image,a method of character truncation was presented to get distinguishing outer contour features.Artificial neural network and features of relations was were used to recognize tire specifications.First,the sample features of distinguishing specifications were gotten:two-thirds characters were intercepted to get run-length characteristics of its external contour.Then,one recognition result was gotten by BP neural network training and recognition.Finally,character features of relations processed fore results were applied to do the second recognition.Experimental results show that the characteristics of acquisition and the second recognition algorithms can effectively identify the tire specifications.Moreover,BP neural network has better fault-tolerance and recognition rate for deformation characters caused by noises,and feature gotten and secondary identification algorithm can effectively identify tire specifications.

A method of tire specification extraction was presented. First, in image pre-processing, iterative least square method was used to fit accurate positioning of the circle center, and coordinate transformation and bilinear interpolation stretching were used to preprocess tire image. Then, window transform was used to reduce image dimensions from two-dimensional to one-dimensional vector, and thus one-dimensional vector was proposed to construct the sawtooth with extreme points. Finally, by using the parameters, the characteristics of the waveform were described to extract character region of tire specifications. Experimental results show that this method can effectively extract the tire specification.

For Intelligent management of car tire production line,a method of fast recognition of car tire size code which combining a drop-in handware system is presented. Tire image is preprocessed including polar transformation,bilinear interpolation stretching and Da-Jing Method to preprocess tire image,segments specifications by connective region. Outline features of size code is extracted based on statistical methods to recognize tire size code. In the drop-in handware system,TMS320C6000 DSP is applied to realize this algorithm,program can be executed quickly by using methods such as soft assembly line,parameter storage and two-buffer memory allocation technology. Experimental result shows that the system method meets the needs of fast recognition of car tire size code.

The Infrared Focal Plane Arrays (IRFPA) imaging system is the main technique tendency of infrared imaging,but the inherent nonuniformity of IRFPA crucially limits its imaging quality. Because the operands and data volume are so vast that it is difficult to realize real-time processing in the Non-uniformity Correction (NUC) on the infrared focal plane. In the drop-in handware system,TMS320C6000 DSP was applied to resolve this problem,which combined Linearity and Second-degree Polynomial segment algorithms. The hardware design and implementation flow of the system was described and the experiment results were given. The results show the real-time non-uniformity correction system meets the real-time and high-precision requirements completely.

To relieve the inefficient of traditional method on auto car tire character recognition based on hand-copying, in this paper, we present an intellective method of fast recognition. Several algorithms were involved in this method, including polar transformation, improved canny operator and distance fast cluster.The procedure was consisted of several steps. In first step, tire image was rotated by polar transformation and mosaic to form a rectangle area by Bilinear Interpolation algorithm. Compared to other methods this part can reduce system time expense greatly. Next, Histogram Equalization algorithm was used to improve the gray distribution and image contrast, which always low on the foreground and background of image for the curing process. Then, the real edge was extracted by improved canny operator, which has advantages of smoothing image, binarizing image and suppressing the big noise caused by rough surface. Subsequently, based on foregoing results, tire image was divided into several small regions, and the judgment of each region whether belongs to real character region was processed by utilizing the continued similarity and the ratio of black pixel number to white pixel number. Furthermore distance fast cluster was utilized to filter noise and split image, and class merging was also used to complete character split. Finally, character feature vectors were extracted and character pattern recognition was completed using them.This method was tested on a series of experiments, the result shows small time expenses and high recognition ratio, which demonstrated that this method can satisfy the special requirement of fast and effective recognition of autocar tire character.