Arachnomelia syndrome (AS) is an autosomal recessive hereditary disorder in cattle, and affected calves are usually stillborn and characterized by complex anomalies. Therefore, identification of the carrier animals based on genetic tests is important for the control and elimination of this defect. The aim of this study was to build an effective workflow to routinely screen the AS mutations in bovine MOSC1 and SUOX genes and determine individuals carrying the AS mutations in four Chinese cattle populations.

Low-temperature conditions influence cattle productivity and survivability. Understanding the metabolic regulations of specific cattle breeds and identifying potential biomarkers related to cold challenges are important for cattle management and optimization of genetic improvement programs. In this study, 28 Inner-Mongolia Sanhe and 22 Holstein heifers were exposed to -25°C for 1 h to evaluate the differences in metabolic mechanisms of thermoregulation. In response to this acute cold challenge, altered rectal temperature was only observed in Holstein cattle. Further metabolome analyses showed a greater baseline of glycolytic activity and mobilization of AA in Sanhe cattle during normal conditions. Both breeds responded to the acute cold challenge by altering their metabolism of volatile fatty acids and AA for gluconeogenesis, which resulted in increased glucose levels. Furthermore, Sanhe cattle mobilized the citric acid cycle activity, and creatine and creatine phosphate metabolism to supply energy, whereas Holstein cattle used greater AA metabolism for this purpose. Altogether, we found that propionate and methanol are potential biomarkers of acute cold challenge response in cattle. Our findings provide novel insights into the biological mechanisms of acute cold response and climatic resilience, and will be used as the basis when developing breeding tools for genetically selecting for improved cold adaptation in cattle.

[目的]探究荷斯坦牛泌乳速度相关指标的群体规律及非遗传因素对其的影响.[方法]收集华东地区某规模化牧场8 757头泌乳牛的906 748条挤奶记录,定义了班次产奶量、前两分钟产奶量、挤奶时间、前两分钟产奶量占比、平均流速和最高流速6个泌乳速度指标,并对其进行了描述性统计,计算了各指标间的Pearson相关系数,采用固定模型分析了测定季节、胎次、泌乳阶段、初产月龄和挤奶班次对泌乳速度相关指标的影响.[结果]6个泌乳速度指标中,除前两分钟产奶量占比之外,班次产奶量、前两分钟产奶量、挤奶时间、平均流速和最高流速均基本呈对称分布,各个指标的变异较大,变异系数在30％左右;各指标两两之间存在极显著相关关系,相关系数在-0.84-0.89之间,泌乳速度与产奶量呈中等或强相关,与挤奶时间呈弱负相关,与前两分钟产奶量占比呈弱正相关.测定季节、胎次、泌乳阶段、初产月龄和挤奶班次对泌乳速度指标均有极显著影响(P＜0.01);不同季节中,冬季的班次产奶量和挤奶时间最大,夏季的平均流速最快,而秋季的最高流速最快;不同泌乳阶段中,在第Ⅱ泌乳阶段(45～99 d),奶牛的班次产奶量、挤奶时间、平均流速和最高流速均最大;不同班次中,在夜间班次(00:00-06:00),奶牛的班次产奶量、挤奶时间、平均流速和最高流速均为最大.[结论]奶牛泌乳速度指标存在较大变异,对季节、胎次等环境和生理因素较为敏感,可为奶牛产奶性能的选育提供信息.

本研究利用2006-2020年间杂交三河牛群体和纯三河牛群体探讨不同外血比例对三河牛生产性能的影响.结果表明,引入德系西门塔尔牛对三河牛的体重、体尺、肉用和产奶性能均有很显著的改良效果.含外血1/2和1/4的三河牛公牛相比纯三河牛公牛,24月龄体重平均增加了11.3kg和5.2kg,屠宰率分别提高3.6％、2.5％,净肉率分别提高了3.8％、1.2％.含外血1/2、1/4和1/8的三河母牛比纯三河牛305d产奶量平均分别提高了125kg、118kg和105kg.很显然,随着外血比例的降低,肉用和乳用性能提升幅度均有所下降.本研究结果为三河牛遗传改良提供了科学依据.

无角是养牛生产中的有利性状,呈常染色体显性遗传,已报道有3种不同的无角基因,分别源自欧洲牛(2种)和蒙古牛(1种).三河牛是经多品种杂交育成的乳肉兼用牛品种,经过长期选育在品种内形成一个无角品系,但其无角基因的具体类型及来源目前尚不清楚.本研究收集7头三河牛种公牛(无角3头、有角4头)和20头蒙古牛(无角2头、有角18头)的样本,使用等位基因特异性PCR技术分别鉴定3种无角基因的基因型.结果显示:3头无角三河牛的无角基因均属于蒙古牛无角基因类型,其中2头为无角杂合子、1头为无角纯合子,2头无角蒙古牛均为蒙古牛无角基因杂合子,样本中未检出欧洲牛无角基因.经PCR产物测序验证,无角三河牛与无角蒙古牛的无角等位基因的核苷酸序列一致.据此判定,三河牛培育过程中虽然有欧洲牛品种参与,但三河牛无角基因来自本地蒙古牛.本研究为三河牛无角品系的分子选育奠定了技术基础.

Inner-Mongolia Sanhe cattle are well-adapted to low-temperature conditions, but the metabolic mechanisms underlying their climatic resilience are still unknown. Based on the 1H Nuclear Magnetic Resonance platform, 41 metabolites were identified and quantified in the serum of 10 heifers under thermal neutrality (5 °C), and subsequent exposure to hyper-cold temperature (−32 °C) for 3 h. Subsequently, 28 metabolites were pre-filtrated, and they provided better performance in multivariate analysis than that of using 41 metabolites. This indicated the need for pre-filtering of the metabolome data in a paired experimental design. In response to the cold exposure challenge, 19 metabolites associated with cold stress response were identified, mainly enriched in “aminoacyl-tRNA biosynthesis” and “valine, leucine, and isoleucine degradation”. A further integration of metabolome and gene expression highlighted the functional roles of the DLD (dihydrolipoamide dehydrogenase), WARS (tryptophanyl-tRNA synthetase), and RARS (arginyl-tRNA synthetase) genes in metabolic pathways of valine and leucine. Furthermore, the essential regulations of SLC30A6 (solute carrier family 30 (zinc transporter), member 6) in metabolic transportation for propionate, acetate, valine, and leucine under severe cold exposure were observed. Our findings presented a comprehensive characterization of the serum metabolome of Inner-Mongolia Sanhe cattle, and contributed to a better understanding of the crucial roles of regulations in metabolites and metabolic pathways during cold stress events in cattle.

The genetic mechanisms underlying the cattle resilience to severe cold temperatures are still unknown. In this study, we observed that four blood biochemical parameters were significantly altered, i.e., blood adrenocorticotropic hormone (ACTH), triiodothyronine (T3), thyroxine (T4), and potassium (K+) after expose to − 32 °C for 3 h. This was observed using 105 healthy Sanhe heifers with similar weight (398.17 ± 34.06 kg) and age (19.30 ± 4.91 months). A total of 20 single nucleotide polymorphisms (SNPs) were identified in 5′-flanking region of the hsp70 gene in Sanhe cattle, while only 10 SNPs were segregating when comparing genetic variations between Sanhe cattle and 285 Chinese Holstein samples. Statistically significant associations between the genomic markers SNP-42−, SNP-105+, SNP-181+, and SNP-205+ with blood T3 and between SNP-105+ and blood T4 were observed by applying the general linear model procedure and Bonferroni t test. Furthermore, we demonstrated that the T alleles of SNP-42− and SNP-205+ in the GC box and Kozak sequence of the hsp70 gene, respectively, significantly decreased the green fluorescent proteins activity in vitro GFP reporter assays. These findings suggest that these two SNPs are causative polymorphisms involved in the regulation of hsp70 promoter activity and might contribute to the observed association between the hsp70 gene and T3 and T4 levels in Sanhe cattle. Thus, hsp70 gene is a promising candidate gene to be validated in independent cattle populations and functional studies related to cold stress resilience in cattle.

三河牛具有适应当地气候、生态条件并且生产性能高的特点,2009年已进入国家良种补贴计划,对当地牛群的遗传改良发挥了重要作用.但由于受客观条件的限制,人工授精技术在呼伦贝尔边远地区尚未普及,为此特为不具备冷配条件的地区特培了用于本交的三河牛公牛,以期为边远牧区提供优良种畜,提高当地牛群遗传水平.经2014～2017年的推广应用,杂种一代犊牛增重速度比当地犊牛高20％以上,而且杂交后代具有与当地牛同样的耐粗饲、耐寒、适应性强的特性,同时母牛的配种受胎率高,大大增加了当地牧民的养殖效益.

Background: Bovine mastitis is the most common and costly disease of lactating cattle worldwide. Apart from milk somatic cell count (SCC) and somatic cell score (SCS), serum cytokines such as interleukin-17 (IL-17) and interleukin-4 (IL-4) may also be potential indicators for bovine mastitis. The present study was designed to investigate the effects of single nucleotide polymorphisms (SNPs) in bovine IL-17F and IL-17A genes on SCC, SCS and serum cytokines in Chinese Holstein and Inner-Mongolia Sanhe cattle, and to compare the mRNA expression variations of the cows with different genotypes.Results: A total of 464 lactating cows (337 Holstein and 127 Inner-Mongolia Sanhe cattle) were screened for SNPs identification and the data were analyzed using fixed effects of herd, parity, season and year of calving by general linear model procedure. The results revealed that SNP g.24392436C > T in IL-17F and SNP g.24345410A > G in IL-17A showed significant effects on SCC and IL-4 in Holstein (n = 337) and on IL-17 and IL-4 in Sanhe cattle (n = 127). The homozygous GG genotype of SNP g.24345410A > G had significantly higher mRNA expression compared with the heterozygous AG genotype.Conclusions: The results indicate that IL-17F and IL-17A could be powerful candidate genes of mastitis resistance and the significant SNPs might be useful genetic markers against mastitis in both dairy and dual purpose cattle.

This study was conducted to establish model for genetic analyses of adult body size and body weight in Sanhe cattle,and to estimate population genetic parameters,in order to provide foundational parameters for establishing breeding objective and forming selection index for Sanhe cattle.Body size and body weight data of 4 491 adult Sanhe cows were measured from 1997 to 2010,a total of 48 946 records were collected from Xiertala Cattle Farm of Inner Mongolia,where the nuclear herd for Sanhe cattle were located,and used for genetic analyses.The 15 body size and body weight traits included body height(BOH),body length (BI),chest girth (CG),cannon circumference (CC),body weight (BW),abdomen circumference (AC),back height (BAH),hip cross height(HCH),chest depth (CD),chest width (CW),hip width (HW),pin bone width (PBW),rump length (RL),head length (HL) and forehead width (FW).DMU package was used for genetic evaluation(AI-REML and EM algorithm) combined with multiple traits animal model,considering age of cow as fixed effect,farm-year-season and additive genetic effect as random effects.Heritabilities and genetic correlations were computed based on estimated variance components,and genetic trends of all traits were analyzed based on estimated breeding values.The results showed that the estimated heritabilities of BOH,BL,CG,CC,BW,AC,BAH,HCH,CD,CW,HW,PBW,RL,HL and FW in adult cows were 0.73±0.02,0.58± 0.02,0.43±0.03,0.51±0.02,0.41±0.03,0.51±0.03,0.60±0.03,0.69±0.03,0.55± 0.03,0.26±0.02,0.28±0.03,0.17±0.02,0.21±0.02,0.69±0.03 and 0.55±0.03,respec-tively.The genetic correlation coefficient among body size and weight traits for adult cows were from-0.19 to 0.92,and their phenotypic correlation coefficient were from-0.30 to 0.93.The genetic correlation coefficient among BOH,BAH and HCH were 0.79-0.88,and their phenotypic correlation coefficient were 0.76-0.93.Genetic correlation among HW,PBW and RL were low (0.11-0.34),and their phenotypic correlation were medium (0.50-0.83).The heritabilities of body size and body weight in adult Sanhe cows are medium to high inheritable traits in general.In which,low genetic correlation exists among traits related to reproduction performance,while higher genetic correlation exists among traits related to body structure.It is suggested that selection system for Sanhe cattle should be strengthened,so that genetic level of economically important traits could be improved steadily.

为了探究三河牛种公牛生长发育规律及拟合生长曲线,试验采用SAS 9.2中的Richards模型对669头三河牛种公牛初生至42月龄体尺体重数据进行拟合分析.结果表明:Richards模型对三河牛种公牛体尺及体重拟合效果较好,拟合度均大于0.98;体重拐点月龄为7.68月龄,拐点体重为224.64 kg.说明Richards模型能够较好地反映三河牛种公牛的生长情况.

为研究从酸马奶中提取的植物乳杆菌DSM20174细菌素的理化特性,本试验提纯植物乳杆菌DSM20174细菌素,经过硫酸铵沉淀、透析后得到细菌素粗提液,再利用SephadexG-100凝胶柱层析进行纯化后,细菌素的比活力明显增加,达到154.70 AU/mL,得率为43.5％.采用牛津杯法测定纯化的植物乳杆菌DSM20174细菌素在3种不同方式处理下对牛源野生致病性大肠杆菌O78抑菌效果的影响.结果显示:①植物乳杆菌DSM20174细菌素在5组温度处理下,随着温度的升高抑菌作用降低,各组抑菌作用差异显著(P＜0.05).即使121℃处理30 min后,抑菌直径仍达14.90mm.②植物乳杆菌DSM20174细菌素在pH 2.0～12.0由低到高的11组酸碱处理下,pH越大抑菌直径越小,且除了pH 9.0和pH 10.0之间差异不显著(P＞0.05)外,其余各组抑菌作用均差异显著(P＜0.05).③植物乳杆菌DSM20174细菌素经胰蛋白酶、胃蛋白酶、木瓜蛋白酶3种酶处理后抑菌直径变化明显,处理前后抑菌作用均差异显著(P＜0.05).结果表明植物乳杆菌DSM20174细菌素是较好的抑菌活性物质,但不是热稳定性物质;具有较广泛的pH适用范围,且pH越小时其抑菌活性越强,在最适生长pH时抑菌活性有所增强;不是蛋白酶稳定性物质.

研究冷应激条件下荷斯坦奶牛和三河牛的维持行为和抗氧化性能的变化规律,为奶牛选育和冬季饲养管理提供参考.选取第2胎的荷斯坦奶牛和三河牛各12头,随机分为4个处理组:分别对舍内(7℃~12℃)和舍外(-30℃~-16℃)荷斯坦奶牛和三河牛进行为期6 d的行为观察和记录,在第7天早晨各组奶牛空腹进行尾部静脉采血测定抗氧化指标.结果表明,舍外冷应激环境条件下,荷斯坦奶牛和三河牛的卧息时间、反刍时间、排尿次数和排便次数显著性增加(P<0.05),站立/游走时间和呼吸频率显著性降低(P<0.05);同时血清T-SOD和CuZn-SOD的活力显著降低(P<0.05),MDA含量显著增多(P<0.05);另外,奶牛品种之间的维持行为和抗氧化性能无显著差异(P>0.05),但总体趋势是荷斯坦奶牛各项测定指标变化略大于三河牛.结果显示,冷应激能够显著改变荷斯坦奶牛与三河牛的躺卧息、反刍和站立/游走等维持行为和抗氧化性能,而三河牛比荷斯坦奶牛更能适应高寒天气.

目前多个国家使用体细胞数作为检测奶牛乳房炎的指标,但是每月的SCC测定日记录不能准确及时反映乳房炎变化程度,而SCC育种值需要大量数据进行估计,不能简易快速地检测隐性乳房炎牛只.因此,本研究通过对内蒙古海拉尔市谢尔塔拉镇2 649头三河牛的20 583条测定日记录进行统计分析,利用SAS 9.1.3软件对三河牛相邻两月SCC差值(分为高SCC差和低SCC差)与日产奶量、乳脂率、乳蛋白率和乳糖率进行相关分析以及高、低SCC差的T检验.结果表明,高SCC差值的奶牛其日产奶量极显著低于低SCC差奶牛(P＜0.01),而且随着胎次的增加,该趋势更加明显.因此,各牛场应通过观察相邻两个月的SCC测定日记录,利用SCC差值法检测高SCC奶牛,以及时发现隐性乳房炎易感牛,从而减少乳房炎引起的经济损失.

Improvement for carcass traits related to beef quality is the key concern in beef production. Recent reports found that epigenetics mediates the interaction of individuals with environment and nutrition. The present study was designed to analyze the genetic effect of single nucleotide polymorphisms (SNPs) in seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in a total of fourteen cattle populations. Sixteen SNPs were identified and genotyped in 383 individuals sampled from the 14 cattle breeds, which included 147 samples from the Snow Dragon beef population. Data analysis showed significant association of 8 SNPs within 4 genes related to carcass and/or meat quality traits in the beef populations. SNP1 (13154420A>G) in exon 17 of DNMT1 was significantly associated with rib-eye width and lean meat color score (p<0.05). A novel SNP (SNP4, 76198537A>G) of DNMT3a was significantly associated with six beef quality traits. Those individuals with the wild-type genotype AA of DNMT3a showed an increase in carcass weight, chilled carcass weight, flank thicknesses, chuck short rib thickness, chuck short rib score and in chuck flap weight in contrast to the GG genotype. Five out of six SNPs in DNMT3b gene were significantly associated with three beef quality traits. SNP15 (45219258C>T) in CAPN1 was significantly associated with chuck short rib thickness and lean meat color score (p<0.05). The significant effect of SNP15 on lean meat color score individually and in combination with each of other 14 SNPs qualify this SNP to be used as potential marker for improving the trait. In addition, the frequencies of most wild-type alleles were higher than those of the mutant alleles in the native and foreign cattle breeds. Seven SNPs were identified in the epigenetic-related genes. The SNP15 in CAPN1 could be used as a powerful genetic marker in selection programs for beef quality improvement in the Snow Dragon Beef population.

对内蒙古三河牛核心群泌乳牛和干奶牛乳房外观洁净度进行全面评分和系统分析,研究影响三河牛乳房外观洁净度的重要部位,分析各部位洁净度评分与体细胞数(Somaticcell count,SCC)和体细胞评分(Somatic cell score,SCS)的相关关系,为三河牛乳房外观洁净度评分体系建立及隐性乳房炎防治提供数据参考.通过采集内蒙古海拉尔区三河牛基础母牛群201头泌乳牛及干奶牛的789份图像信息,在建立三河牛乳房外观洁净度评分体系基础上,对三河牛左后肢、左躯体、左乳区、右后肢、右躯体、右乳区、后乳区和尾部等8个部位进行5个等级的评分,同时进行整体洁净度评分,并运用SAS 9.1.3进行了系统的统计分析.三河牛8个部位洁净度评分之间存在极显著相关(P＜0.01),其中左后肢、左乳区、右乳区、后乳区和尾部等5个部位极显著影响整体洁净度评分(P＜0.01).通过主成分分析将8个部位洁净度评分变量降维至3个主成分.逻辑回归分析和方差分析得出,三河牛体况评分对SCC有极显著影响(P＜0.01),右乳区评分对SCC有显著影响(P＜0.05),胎次效应对SCS影响极显著(P＜0.01).本研究建立了包含8个部位的三河牛乳房外观整体洁净度评分体系(5分制),其主要部位是右后肢、右乳区和尾部,尤其是右乳区洁净程度.右乳区评分对隐性乳房炎指标SCC的显著影响提示其有望作为预防三河牛隐形乳房炎发生的直观选择指标.

二酰甘油酰基转移酶(DGAT1)基因K232A位点是一个与奶牛产奶性能有关的重要位点.本研究通过对三河牛K232A位点检测发现,该位点在三河牛中处于Hardy-Weinberg平衡状态,说明没有经过选择.KK基因型个体具有最低的体细胞评分和最高的乳脂率;KA基因型个体具有最高的产奶量和乳脂量;AA基因型个体具有最高的乳蛋白量.K等位基因有利于提高乳脂率和乳脂量,同时降低体细胞评分.在三河牛群体中,K等位基因的基因频率为0.23,利用K位点来提高乳脂率和乳脂量以及降低体细胞评分,存在很大的选择空间.

The Sanhe cattle is a domestic dual purpose breed formed and approved in China in 1983, which was named after its origin of Sanhe region in Hulunbuir pasture land. Sanhe cattle is famous for its durability, suitability for grazing, cold resistance and strong adaptability. This study was conducted to estimate the marginal profits of traits in breeding goal and provide a base for designing breeding plan in Sanhe cattle. [Method] The performance, economic and nutrition parameters of Sanhe cattle were obtained according to theoretical nutrition demand of dairy and beef cattle, as well as the production and breeding data in 2011 from Xiertala Cattle Breeding Farm in Inner Mongolia, China, which are the central production region of this breed. Also, the breeding objective traits were proposed based on the production and breeding system of Sanhe cattle. A bio-economic profit model was fitted using Profit Equation method, and the marginal profits were calculated for the milk performance, beef performance and functional traits, then the sensitivity analysis was run.[Result]The results indicated that ten breeding objective traits for Sanhe cattle included three milk performance traits (milk yield, fat percentage, and protein percentage), three beef performance traits (birth weight, fattening daily gain, and dressing percentage), and four functional traits (somatic cell count, age at first calving, calving interval, and productive life). The total profit of Sanhe cattle was RMB 4 808.26 with a fixed scale of the population. The profit generated by milk production, culling cows and fattening bulls account for 67.08%,7.8%and 20.58%of the total profit, respectively. For the cost, the variable cost accounts for 96.89%of the total cost. Feeding cost is the main factor affecting the variable cost. The feeding cost of the heifers, milking cows and fattening bulls account for 45.69%, 20.46%, and 13.15% of the total cost, respectively. When raw milk price considering milk compositions and quality (fat percentage, protein percentage, and somatic cell count), the marginal profits of objective traits in Sanhe cattle were RMB 2.26 (milk yield, MY, kg), RMB 40.48 (fat percentage, FP,%), RMB 61.39 (protein percentage, PP,%), RMB-231.52 (somatic cell count, SCC), RMB 6.19 (birth weight, BW, kg), RMB 1.39 (fattening daily gain, FDG, g/day), RMB 76.70 (dressing percentage, DP,%), RMB 0.25 (productive lifetime, PL, day), RMB-1.62 (age at first calving, AFC, day), and RMB-2.71 (calving interval, CI, day),respectively. The relative economic weights for milk performance, beef performance and function traits is 62﹕14﹕22, closing to 3﹕1﹕1. With the increase of 10%of the milk price, the marginal profits of MY and CI increased by RMB 0.29, RMB 0.17, respectively;with the increase of 10%of the beef price, the marginal profits of BW, FDG, DP and CI increased by RMB 0.81, RMB 0.15, and RMB 7.86, respectively and PL decreased by RMB 0.03;with the increase of 10%of the feed price, the marginal profits of MY, FP, PP, BW, AFC, FDG decreased by RMB 0.05, RMB 0.58,RMB 0.34, RMB 0.21, RMB 0.16, and RMB 0.01, respectively and CI and PL increased by RMB 0.88 and RMB 0.06, respectively. [Conclusion]Change of market price would largely affect the marginal profits of milk and beef production in Sanhe cattle. The bio-economic profit model derived from this study will provide a reference for dual purpose cattle breeding in China and a basis for development of breeding goals for Sanhe cattle in the future.

牛蜘蛛腿综合征(Arachnomelia syndrome,AS)是一种隐性遗传疾病,虽然在瑞士褐牛和西门塔尔牛中症 状相同,然而却是由两个不同的基因突变引起的,分别是SUOX 基因c.363-364insG 突变和MOCS1 基因 c.1224_1225delCA 突变.文章利用51 头西门塔尔牛及80 头与配母牛和106 头杂交后代,以及55 头新疆褐牛 公牛为研究群体,通过荧光标记引物PCR 扩增结合毛细管电泳技术,建立了一种荧光自动化检测方法,能够快 速、准确、一次性实现对引起牛AS 疾病的两个突变位点的同时检测,为今后我国牛群中AS 致病位点的筛查 工作奠定了基础.

【Objective】The aim of this study is to detect Y367C site in ATP-binding cassette super-family G member 2(ABCG2) gene in Sanhe cattle and analyze its association with milk production traits.【Method】The detection of the site was analyzed with Sequenom Mass ARRAY Genotype technology.The association between the site and 6 milk production traits was analyzed with software SAS(version 9.1).【Result】The results showed that Y367C conformed to Hardy-Weinberg Law(P0.05).Individuals with YY genotype of Y367C site had the lowest somatic cell score;while those with CC genotype had the highest milk yield,fat yield,and protein yield.Allele Y could improve somatic cell score and protein percentage.Allele C could improve milk yield,fat percentage,fat yield and protein yield,but there was no significant difference among three genotypes(P0.05).【Conclusion】The site could provide reference for molecular breeding in Sanhe cattle.