目的 基于Wnt信号通路探讨烙灸疗法对大鼠脊髓损伤后神经修复的影响.方法 将60只雄性SD大鼠随机分为假手术组、模型组、烙灸组、西药组、Wnt抑制剂组和Wnt抑制剂+烙灸组,每组10只.除假手术组外,其余组大鼠采用撞击T9椎板方法建立脊髓损伤模型.烙灸组于建模后24 h在损伤部位局部进行烙灸,1次/d,每次5 min;西药组于建模后0.5 h给予20 mg/kg醋酸泼尼松注射,之后按照4.9 mg/(kg·d)继续干预;Wnt抑制剂组于建模后0.5 h在损伤部位皮下注射40 μg/(kg·d)的Wnt通路抑制剂IWR-1;Wnt抑制剂+烙灸组于建模后0.5 h在损伤部位皮下注射40 μg/(kg·d)的IWR-1,然后进行烙灸.各组均连续干预28 d.采用BBB运动评定量表和斜板试验评估大鼠运动能力,HE染色观察脊髓组织病理学形态,免疫组化法和Western blot法检测脊髓组织中巢蛋白(Nestin)、黏附连接蛋白(β-catenin)和神经胶质纤维酸性蛋白(GFAP)、糖原合成激酶-3(GSK-3β)表达情况.结果 随术后时间延长,除Wnt抑制剂组的BBB评分和斜板角度无明显变化外,其余各造模组大鼠的BBB评分和斜板角度均呈递增趋势;其中烙灸组和西药组大鼠术后第7,14,21,28天的BBB评分均明显高于同期模型组、Wnt抑制剂组、Wnt抑制剂+烙灸组(P均＜0.05),术后第21,28天的斜板角度均明显高于同期模型组、Wnt抑制剂组、Wnt抑制剂+烙灸组(P均＜0.05).HE染色显示模型组、Wnt抑制剂组大鼠脊髓严重损伤,细胞坏死,神经元数量明显减少;烙灸组、西药组大鼠脊髓损伤明显轻于模型组、W n t抑制剂组、W nt抑制剂+烙灸组.Nestin、β-catenin、GFAP和GSK-3β在脊髓的白质和灰质中都有表达;与假手术组比较,模型组、Wnt抑制剂组和Wnt抑制剂+烙灸组脊髓组织中Nestin和GFAP蛋白表达量均明显升高(P均＜0.05),β-catenin和GSK-3β蛋白表达量均明显降低(P均＜0.05);与模型组比较,西药组和烙灸组脊髓组织中Nestin、β-catenin、GSK-3β蛋白表达量均明显升高而GFAP蛋白表达量均明显降低(P均＜0.05),Wnt抑制剂+烙灸组β-catenin蛋白表达量明显升高(P＜0.05).结论 烙灸疗法可通过激活Wnt信号通路促进脊髓损伤大鼠神经修复.

目的：探讨互联网+三维动画技术联合Workshop混合式教学方式在中医骨伤科学课程中的教学效果。方法：选取本校2018年级30名针推本科专业学生作为研究对象，设为观察组，采用互联网+三维动画技术联合Workshop混合式教学方式授课；另选取本校2016年级29名针推本科专业学生作为对照组，运用理论课堂授课方式进行授课，比较两组学生考试成绩及其对授课方式满意度。结果：观察组学生期末技能操作成绩高于对照组（P<0.05），期末笔试成绩略有提高，差异无统计学意义（P>0.05）；对照组学生对教学方式满意程度为82.76%，观察组为96.67%，观察组高于对照组，差异有统计学意义（P<0.05）。结论：互联网+三维动画技术联合Workshop混合教学方式在中医骨伤科学中的教学效果得到肯定，显著提高学生技能考试成绩，笔试成绩略有提高，提升了教学方式效果，并拓展了知识空间，提高了学生学习热情，值得推广。

目的:基于Wnt、Notch信号通路分析烙灸对脊髓损伤后大鼠的神经修复作用.方法:将60只大鼠按照随机数表法分为假手术组、模型组、甲基强的松龙组、2 h烙灸组、12 h烙灸组,采用改良Allen's模型打击装置,进行急性脊髓损伤造模.甲基强的松龙组于损伤后0.5 h给药,首次30 mg/kg,之后5.4 mg/kg给药.2 h烙灸组、12 h烙灸组分别于损伤后2 h、12 h进行烙灸治疗.分别在造模成功后第1d、第7d、第14d、第21d进行脊髓组织取材,利用Westernblot进行蛋白表达量的检测.结果:在Wnt1、Wnt3 a蛋白检测中,2 h烙灸组表达量明显低于12 h烙灸组,差异具有统计学意义(P<0.05);2 h烙灸组的Hes5、Presenilin1蛋白高于12 h烙灸组,差异具有统计学意义(P<0.05).结论:烙灸在脊髓损伤后激活Wnt及Notch信号通路,而Wnt与Notch信号通路间相互作用,可能影响烙灸治疗后相关因子的蛋白表达量.

目的:观察张氏正骨技术(小夹板固定)与张氏正骨技术配合石膏托外固定治疗肱骨干骨折的临床疗效.方法:采取回顾性方法对162例肱骨干骨折患者的临床资料进行分析,观察组采用张氏正骨技术,对照组采用张氏正骨技术配合石膏托外固定,两组患者均进行肩关节、 肘关节功能评分比较两组的愈合情况及治疗3个月后的疗效评价.结果:观察组肩关节功能评分、 肘关节功能评分均优于对照组,差异有统计学意义(P<0.05).3个月后疗效评价比较,观察组优良率为85.29％,对照组优良率为81.67％,差异有统计学意义(P<0.05).结论:张氏正骨技术治疗肱骨干骨折疗效较好,肱骨功能恢复快且操作简单安全,并发症少,费用低廉,值得在基层推广.

目的 了解宁夏回族自治区社区卫生管理人员全科医学知识掌握情况及培训需求,为今后培训提供依据.方法 采用现场自填式问卷调查,对参加宁夏社区卫生管理人员培训班的学员进行调查.结果 不同年龄段和不同社区卫生管理工作年限的学员全科医学知识的分数差异有统计学意义(P＜0.05);年龄在31岁～组和41岁～组全科医学知识的分数最高.社区卫生管理工作年限在1～5年和6～10年的全科医学知识的分数最高.学员对社区卫生服务相关知识的掌握情况中,掌握最好的前5位分别为,慢性病管理(84.9％)、人际交流和沟通(74.5％)、公共卫生管理(71.7％)、传染病处理(71.7％)、全科医学概论(70.8％).认为有必要培训的知识从高到低进行排序,前五位分别为心理疾病防治(70.8％)、社区康复技能(68.9％)、老年医学(65.1％)、新的医药知识(63.2％)、人力资源管理(62.3％)和心理学基础(62.3％).结论 宁夏社区卫生管理人员对全科医学知识的掌握情况较好,心理疾病防治、社区康复技能和管理学知识有较高的培训需求.

Objective Effect of Hui medicine Lao moxibustion on expression of Presenilin1 and Hes1 in notch signaling pathway in spinal cord injury rats. Methods Allen's method was used to make the rats’ model of acute spinal cord injury. Experimental animals were divided into fake operational group (group A), model group (group B), treatment group by western medicine (group C), treatment group by Hui medicine Lao moxibustion two hours after manufacturing model (group D), treatment group by Hui medicine Lao moxibustion six hours after manufacturing model (group E). On the 1st, 7th, 14th and 28th day, the injured spinal cord tissue was taken. The expression of presenilin1 and hes1 in the spinal cord rats was detected by immunohistochemistry. Results Presenilin1, Hes1 immunohistochemical staining showed that the D and E groups were less than B and C, and the expression rate of group D was the most significant, and the difference was statistically significant (P＜0.01). Conclusion By inhibiting the expression of Notch signaling pathway, Hui medicine Lao moxibustion therapy promoted the differentiation of endogenous neural stem cells into neuron cells and oligodendrocytes in order to promote the repair of local nerves, and the effect of pre-Lao moxibustion intervention was better than that of later intervention.

目的:探讨回医烙灸疗法对脊髓损伤大鼠Wnt信号通路中Wnt-1、β-catenin蛋白表达的影响.方法:采用Allen'制备急性脊髓损伤模型,将造模成功的75只大鼠随机分为假手术组、模型组、西药组、2h烙灸组、6h烙灸组,每组各15只.于第1天、第7天、第14天、第28天取损伤大鼠脊髓组织,免疫组化法检测大鼠脊髓组织中Wnt-1、β-catenin蛋白表达.结果:第1天、第7天、第14天、第28天,模型组、西药组、2h烙灸组、6h烙灸组Wnt-1及β-catenin蛋白表达平均光密度均大于假手术组,差异有统计学意义(P＜0.01).第7天、第14天西药组、2h烙灸组、6h烙灸组均大于模型组,差异有统计学意义(P＜0.01),西药组、2h烙灸组、6h烙灸组间比较,差异无统计学意义(P＞0.05);损伤第28天,2h烙灸组、6h烙灸组大于模型组、西药组,且2h烙灸组表达率最显著,差异有统计学意义(P＜0.01).结论:回医烙灸疗法通过上调脊髓损伤大鼠局部Wnt-1、β-catenin的表达,从而上调Wnt信号通路的表达,使其局部神经干细胞增殖分化,以促进其局部神经的修复,且前期烙灸干预的效果好于后期干预.

目的 探讨高中生睡眠质量与血压的关系,为预防青少年高血压提供依据.方法 对整群抽取的宁夏银川市某高中2 116名学生,测量血压、身高、体重,并进行匹兹堡睡眠质量指数量表(PSQI)问卷调查.结果 银川市某高中学生睡眠质量差的检出率为38.04％.不同主观睡眠质量、催眠药物使用、睡眠质量的高中生血压水平差异均有统计学意义(P值均＜0.05).睡眠时间在＞7 h和≤7h之间血压水平差异无统计学意义(P＞0.05).在调整年龄、性别、体育锻炼、生源地、体质量指数、吸烟、饮酒后的多元线性回归中,睡眠质量与收缩压(β=2.68,P＜0.01)、舒张压(β=0.75,P=0.02)的关联均存在统计学意义.结论 睡眠质量和高中生血压有关.提高睡眠质量可能有利于预防青少年高血压.

Objective:To observe the clinical effect of the treatment of clavicle fractures with the technique of Hui medicine.Methods:The pain,swelling,counterpoint,line,length and amount of callus at different time points of 100 cases of patients in Ningxia Hui Medical Orthopaedics Hospital were socred and then after 3 months of treatment the curative effect was evaluated.Results:Pain,swelling,counterpoint,line and length were significantly lower than those before treatment,and the difference was statistically significant (P ＜ 0.05).After treatment and 1,2,4,6 and 8 weeks after treatment,compared with gradual improvement,the difference was statistically significant (P ＜ 0.05).The amount of callus,in addition to no significant improvement before and after treatment(P ＞ 0.05),the difference was statistically significant (P ＜ 0.05).After 3 months of treatment,the efficacy was 96％.Conclusion:Hui medicine ZHANG's bone-setting technology can effectively treat clavicle fractures and the curative effect was distinct,worthy of promotion.

Objective:To observe the clinical effects of Zhang's bone-setting technique of Hui medicine on tibia and fibula fracture.Methods:Totally 80 patients with closed tibia and fibula fracture treated in the Wuzhong Bone-Setting Hospital of Hui Medicine between January 2013 and September 2015 were given treatment of Zhang's bone-setting technique.The scores of pain,swelling,positional alignment,linear,length and osteotylus quantity were determined,and the efficacy 6 months after treatment was evaluated.Results:After 6 months of treatment for the 80 patients,67 cases had excellent effects,10 cases with good effects,2 cases with effects,1 case with poor effect;the excellent and good effects were 96.25％.The scores of pain,swelling,positional alignment,linear and length declined markedly compared with those before treatment;the difference had statistical significance (P ＜0.05).The osteotylus quantity had no obvious improvement compared with that before treatment,without statistical difference (P ＞ 0.05).The osteotylus quantity 1,2,4 and 8 weeks after treatment had obvious improvement compared with those before treatment,with statistical difference (P ＜ 0.05).Conclusion:Zhang's bone-setting technique of Hui medicine is effective for the treatment of tibia and fibula fracture.

Objective:To observe the regulatory mechanism of Lao moxibustion in Hui medicine on the gene expression of the rats with spinal cord injury,and explore the relations between regulatory genes and protection,repair,growth and regeneration of nervous tissues.Methods:Allen's method was applied to make the rat models with acute spinal cord injury.Experimental animals were divided into sham operational group (group A),injury group (group B),Lao moxibustion group (group C).The gene expression of the rats with spinal cord injury had been analyzed by using the expression profile gene chip,screened up-regulated first and down-regulated as well as down-regulated first and up-regulated genes,and the expression level of 8 genes were verified by RT-PCR.Results:Twenty-four genes were screened,13 genes were first up-regulated and then down-regulated,11 genes were first down-regulated and then up-regulated.Its function mainly involved cell metabolism and apoptosis,ion channels,a variety of proteins,cell cycle and growth regulation,etc.Conclusion:Lao moxibustion of Hui Medicine has certain regulation effect on the gene expression of spinal cord injury rats.The regulated genes are closely related to the protection,repairmen,grow and regeneration of the nervous tissues.

回医学本科专业是我校特色新专业,具有浓郁的民族地域特色.通过对回医学专业及回医学方向本科生调研发现,回医学本科专业在课程设置、回医学系列教材建设、回医学诊疗技术等方面存在问题,故对相关问题进行分析探讨,并提出了解决对策,以期待提高回医学人才培养质量.

Objective:To study the influence of Hui medicine Lao moxibustion on the gene expression profiles in the middle term of spinal cord injury in rats.Methods:Allen's method was used to make the rats' model of acute spinal cord injury.Experimental animals were divided into fake operational group (group A),injury group(group B),Hui medicine Lao moxibustion group (group C).The gene expression profiles of the rats which 14 days after spinal cord injury have been analyzed by using the expression profile gene chip and the expression level of eight genes were verified by RT-PCR.Results:The gene expression of Hui medicine Lao moxibustion group was significantly different.The up-regulated genes mainly included many kinds of proteins,transcription factors,chemokines and signaling pathways.Down-regulated genes included ion channels,a variety of proteins,transcription factors,nucleotides,a variety of enzymes,etc.Conclusion:Hui medicine Lao moxibustion can significantly modulate the gene expression of the rats in the middle term of spinal cord injury.Some genes are closely related to neural tissue protection,repair,growth and regeneration so as to reduce the secondary injury of spinal cord.

目的 观察回医张氏正骨技术治疗肱骨外科颈骨折的临床效果.方法 对宁夏吴忠回医正骨医院收治的90例患者不同时间点疼痛、肿胀、对位、对线、长度、骨痂量进行评分,然后对治疗后4个月疗效评价.结果 疼痛、肿胀、对位、对线、长度与治疗前相比明显降低,差异有统计学意义(P＜0.05);治疗后当天、治疗后1、2、4、6、8周相比逐步改善,差异有统计学意义(P＜0.05).骨痂量除治疗前与治疗后当天相比无明显改善,差异无统计学意义(P＞0.05),其余相比骨痂量明显改善,差异有统计学意义(P＜0.05).治疗后4个月疗效评价显示治疗优良率为95.55％.结论 回医张氏正骨技术能有效治疗肱骨外科颈骨折,疗效显著,值得推广.

Objective:To study the influence of Hui medicine Lao moxibustion to the recovery of neural function of rats of spinal cord injury.Methods:Allen's method was used to make the rats' model of acute spinal cord injury.Experimental animals were divided into fake operational group (group A),only model group (group B),hormone group (group C),treatment group by Hui medicine Lao moxibustion two hours after manufacturing model(group D),treatment group by Hui medicine Lao moxibustion six hours after manufacturing model (group E).Fifty of them in 7,14,28,42,56 days using modified Ravlin inclined plate experiment and cortical somatosensory evoked potential CSEP detection.Another 25 damaged spinal cord tissues were used to make the electron microscope ultrastructure in the spinal cord and TUNEL staining cells apoptosis rates.Results:Ravlin,CSEP,electron microscope and TUNEL staining cell apoptosis rates showed that the effect of D and E was significantly better than that of B and C group (P ＜ 0.05) and the effect of D group was most significant.Conclusion:Hui medicine kao moxibustion can promote the effect on the recovery of neural function of rats' spinal cord injury.The effect of treating by Hui medicine Lao moxibustion in early stage is better than that in later stage.

Objective To explore on the therapeutical effect of Hui Medicine Lao Moxibustion on adjuvant arthritis in rats.Methods Adjuvant arthritis rat model was employed. 60 rats were divided into normal group, model group,methylprednisolone group,Hui Medical Lao Moxibustion group,each group of 15 rats. Body weight,foot metatarsus swelling degrees,and arthritis index were measured in 10 rats of each group while foot metatarsus and knee joint synovial tissue HE staining were performed in another 5 rats of each group for observing the morphology changes of of adjuvant arthritis rats.Results ①The rats weight of model group, methylprednisolone group and Hui Medicine Lao Moxibustion group were significantly lower than that of the normal group at the 10th and 30th day(P<0.05). At the 10th day,the body weight of the rats in the methylprednisolone group and the Lao Moxibustion group were significantly lower than that in the model group. At the 30th day,the body weight of themodel group,methylprednisolone group and Lao Moxibustion group increased gradually(P<0.05).②The swelling degrees of the model group,methylprednisolone group and moxibustion group were significantly higher than that of the normal group at the 10th and 30th day(P<0.05). At the 30th day,the swelling degree of the model group,the methylprednisolone group and the Lao Moxibustion group decreased gradually(P<0.05). ③The adjuvant arthritis index of the methylprednisolone group and the Lao Moxibustion group were significantly lower than that of the model group at the 17th and the 30th day while the adjuvant arthritis index of the Lao Moxibustion group was significantly lower than that of the methylprednisolone group(P<0.05). ④HE staining of the foot metatarsus and the knee joint synovial tissue showed that the damage of bone,cartilage and synovial tissue in model group was more serious at the 17th day after the model establishment. The degree of damage of methylprednisolone group and Lao Moxibustion group was significantly lighter than that of model group.Conclusion Hui Medical Lao Moxibustion has a therapeutical effect on adjuvant arthritis in rats.

Ningxia province health department in May and June 2012 for the community health service team training, team from ningxia city of 5 students, now students choose one yinchuan and carries on the training effect evaluation of each work.

目的 探讨回医烙灸疗法对大鼠脊髓损伤后神经修复功能的影响.方法 采用Allen's法制备急性脊髓损伤模型.实验动物分为假手术组(A组)、单纯损伤组(B组)、西药组(C组)、造模2h烙灸组(D组)、造模6h烙灸组(E组),其中50只于1、2、4、6、8周采用脊髓损伤运动功能BBB法及感觉功能Reuter法评分,另外25只取损伤脊髓组织,作HE及尼氏体染色,观察脊髓损伤后组织形态的变化.结果 BBB及Reuter评分表明D、E组效果优于A、B、C组,且D组疗效最显著.HE及尼氏体染色图像显示,D、E组脊髓形态好于B、C组.结论 回医烙灸疗法对脊髓损伤大鼠神经功能的恢复有促进作用,且前期回医烙灸干预的效果好于后期干预.

目的：观察枸杞多糖治疗创伤性脊髓损伤中后期的临床疗效和安全性。方法：将38例创伤性脊髓损伤中后期患者根据随机的原则分为2组，对照组18例使用常规激素治疗，观察组20例在常规激素治疗的基础上加用枸杞多糖，2组均以14天为1疗程，1疗程后比较两组的临床疗效。结果：总有效率观察组为95%，对照组为88.8%，两组总有效率比较，差异有显著性意义（P<0.05），观察组的疗效优于对照组。结论：枸杞多糖是一种治疗创伤性脊髓损伤安全有效的药物，临床上建议推广运用。

对情景模拟与PBL(problem based learning, PBL)教学模式相结合在骨科教学中的应用进行了初步探索。对教学的实施方法、教学效果和教学体会及其注意事项,以及目前条件下存在的问题和亟待改进方面进行了探讨。为情景模拟与PBL相结合的教学模式在骨科教学中的应用提供经验。