OBJECTIVE:Traditional biochemical testing is limited by fixed sites, specialized equipment, and long turnaround times, severely reducing patient compliance with regular monitoring. To address the demands of routine lipid management, this study developed a simple, rapid and low-cost method for Total Cholesterol (TC) detection that enables high-throughput parallel processing of multiple samples. METHOD:This method synchronously identifies the RGB values of multiple pixels in an image through a mobile phone camera. All experiment procedures were strictly followed according to the guidelines of the National Committee for Clinical Laboratory Standards. Multiple performance indicators of the device were evaluated, and the results were compared for consistency with those from a fully automated clinical biochemical analyzer. RESULTS:The TC concentration showed the strongest quantitative correlation with the green channel intensity (G-value). This method was preliminarily validated on a prototype smartphone. Both its detection precision and accuracy met clinical standards. The method demonstrated satisfactory linearity over 1.19-8.49 mmoL/L, and its analytical results for the limited samples in this study were highly consistent with those obtained from the automatic biochemical analyzer. CONCLUSIONS:Integrating low cost and high efficiency, this novel method has great application potential in indoor settings within primary healthcare institutions and resource-limited remote areas.

Barcode technology has gained significant attention in analytical and bioanalytical chemistry assays due to its high-throughput capability and rapid response. However, early studies primarily focused on the integration of barcode formatting without encoding and interpretation for multiplex assay reading. As a result, the full potential of barcode technology in enhancing assay efficiency and capability has not been fully realized. In this work, a Lego-based, Quick-Response (QR) code-formatted bioassay device has been designed and tested for the detection of disease biomarkers and other potential molecular targets of interest. Particularly the QR-coded device for multiplex immunoassays is consisted of 1 x 1 standard Lego bricks (either white or black), except for those in the "detection zones"; these are replaced by 3D-printed transparent Lego bricks with mini-vials at the center for performing assay reactions. This integrated smart device can be read with a standard barcode app, and the results are processed for semiquantitative readout. This Lego-assembled QR-coded assay device is capable of analyzing up to 24 samples simultaneously within 30 min, and its performance is demonstrated by simultaneously detecting a set of coronary heart disease markers, i.e., triglycerides, total cholesterol, and uric acid. The performance of this portable device meets biomedical testing standards, and its accuracy is validated by comparing real sample data with those obtained on an automatic immunoassay analyzer at hospital. We envision that the developed QR-coded Lego assay device promises a simple, accurate, and rapid platform methodology for point-of-care medical diagnosis and on-site chemical analysis.

Chronic kidney disease is a progressive condition, and serum creatinine (CR) levels are closely associated with the glomerular filtration rate, serving as a key indicator of renal function and disease progression. This study aimed to develop a smartphone-based colorimetric analysis system for the efficient and rapid quantification of serum CR and validate its performance to determine whether the method meets clinical testing standards. The CR standard solution was analyzed using a smartphone, and the R, G, and B values were plotted against the concentration. The precision, accuracy, detection limit, linear range, and clinically reportable range of the smartphone detection system were evaluated according to the National Committee for Clinical Laboratory Standards guidelines. Subsequently, 65 serum samples from healthy individuals and 26 serum samples from nephropathy patients were collected and tested using the smartphone system and an automated biochemical analyzer, respectively, to further validate the feasibility of the method. Among all the color channels, the G value showed the strongest correlation with CR concentration, and therefore was used to establish the standard curve. The validation of the assay system demonstrated that its precision and accuracy met clinical standards. The limit of blank, limit of detection, and limit of quantification were 29.95 μmol/L, 32.39 μmol/L, and 36.61 μmol/L, respectively. The linear range was 36.75 to 200.46 μmol/L, whereas the clinical reporting range spanned from 36.61 to 801.84 μmol/L. Furthermore, the results obtained from the 2 methods were statistically analyzed, revealing a strong correlation between the 2 sets of data. Smartphone-based serum CR testing meets clinical standards, and its portability and efficiency position it as a valuable tool for screening and monitoring chronic kidney disease.

The urine protein-to-creatinine (P/C) ratio is a rapid and accurate method for measuring proteinuria in the diagnosis of kidney diseases. A smartphone device enables rapid determination of creatinine and proteins in urine. A smartphone-based method was developed and validated for the detection of the P/C ratio in this study. Clinical urine samples were also analyzed and compared to the Beckman Coulter AU5800 results for correlation assessment. For proteinuria, the limit of blank (LOB) and limit of detection (LOD) were 4.23 and 8.8 mg/dL, respectively, with a lower limit of quantification (LOQ) of 9.2 mg/dL and a linear range from 9.2 to 122 mg/dL. The intra-batch precision coefficient of variation (CV) was 2.5

In this work, we have developed a portable laser scanning imaging system derived from Blu-ray disc technology for high-throughput biochip and sensor array characterization. This portable imaging system was constructed by adapting a standard Blu-ray drive to perform the scanning or imaging of an entire disc or interested regions. In addition, we have compiled a complete operating software program to process the raw data, generate the assay image, and perform quantitative analysis. Furthermore, the developed BD-LSI system was successfully applied to the quantitative detection of Anti-M & uuml;llerian hormone (AMH), which is an important biomarker of reproductive potential, in standard physiological buffers and serum samples. The achieved detection limit is as low as 18 pg/ mL with a dynamic range of up to 25 ng/mL, which meets the needs of clinical diagnosis. As validated by comparing with the Clinical Laboratory Improvement Amendments (CLIA) data on the same set of samples, the consistent results confirm that this BD-LSI system holds promise for multiplex on-site quantitative analysis at community clinics and even at home settings for self-monitoring of individual health.

Herein, a smartphone-based portable reader with integrated optics for standard microtiter plates (96 wells) has been designed and demonstrated for high-throughput quantitation of validated biomarkers in serum. The customized optical attachment was simply constructed with a convex lens and a light source, by which the transmitted light through a 96-well microtiter plate was converged for imaging with a smartphone, so that accurate and wide-range reading of the plate can be achieved. More importantly, relying on the digitized colorimetric analysis of the obtained images, concentrations of various biomarkers can be determined directly using the customized mobile app. A set of validated biomarkers for inflammation and infection, C-reactive protein (CRP), serum amyloid A (SAA), and procalcitonin (PCT) have been quantitated with this new system; both the response ranges and limits of detection meet the requirement of clinical tests. The consistency with the results obtained using a commercial microplate reader proves its reliability and precision, augments its potential as a point-of-care diagnostic device for on-site testing or resource-limited settings.

线粒体是除成熟红细胞外普遍存在的细胞内细胞器,是真核细胞能量产生的中心,线粒体DNA(mitochondrial DNA,mt DNA)作为线粒体的小基因组在维持线粒体功能的稳定和细胞代谢中扮演着重要的角色.越来越多的实验证据表明线粒体在肿瘤发生、发展中具有重要作用,目前大多数学者认为线粒体是肺恶性肿瘤和诸多实体恶性肿瘤的重要参与者,且mt DNA有望成为潜在的肺恶性肿瘤生物标志物以用于筛查肺恶性肿瘤及预测肺恶性肿瘤分型、分期、治疗疗效及其预后.本文就mt DNA在肺恶性肿瘤发生发展及治疗过程中的作用做一综述,为肺恶性肿瘤的临床诊断及治疗提供帮助.

The seven-span G protein conjugate receptor 183(GPR183), also known as Epstein-Barr virus-induced gene 2(EBI2), is induced by the Epstein-Barr virus. GPR183 is activated by a set of oxidative sterol endogenous ligands, which cooperates with chemokine receptors to control the migration of immune cells. In recent years, studies have shown that GPR183 plays an important role in the immune system, not only participating in adaptive immune response, but also closely related to human diseases. Its expression disorders may lead to the occurrence of diseases including inflammatory diseases, autoimmune diseases and malignant tumors. This review summarizes the function of GPR183 and its immune regulation mechanism and role in immune-related diseases.

细菌整合子具有独特的结构,能够捕获和表达外源基因,通过转座子和质粒可以在细菌间水平传播.多重耐药细菌的出现是临床抗感染治疗面临的巨大挑战,而耐药细菌多携带整合子.因此,整合子的早期检测对尽早判断细菌耐药非常重要.目前,检测细菌整合子的方法主要是基于整合子的整合酶及其基因盒.文章对基于聚合酶链反应(PCR)的各种检测方法和环介导等温扩增(LAMP)、宏基因组测序(mNGS)、基因芯片等细菌整合子检测方法,以及各种检测方法的原理和特点进行介绍,并比较每种方法的优缺点,帮助临床实验室选择更适合的检测方法,及时了解细菌携带整合子的情况,尽早确定细菌耐药特点,为临床诊治提供参考.

[目的 ]探索金属离子对整合子捕获耐药基因盒的影响及其相关机制.[方法]在大肠埃希菌中构建一个1类整合子捕获耐药基因盒的体内模型.通过实时荧光定量PCR(real-time fluorescence quantitative PCR,RT-qPCR)测定不同浓度银(0.3、0.9、1.5 μg/mL 的 Ag+)和铜离子(5、50、100、150、210 μg/mL的Cu2+)干预后实验组和无金属离子干预对照组整合子整合频率,并用表型筛选法验证.利用质谱法测量细菌吸收的金属离子浓度,并进一步通过转录组测序方法分析银离子抑制细菌整合子捕获耐药基因盒的分子机制.[结果]qPCR和表型筛选法的结果表明,0.9 μg/mL银离子组整合频率为9.42×10-5(6.49×10-5,1.44×10-4),1.5 μg/mL银离子组整合频率为7.29×10-5(4.45×10-5,9.03×10-5),与对照组 2.59×10-4(2.24×10-4,3.33×10-4)相比整合频率明显降低,差异有统计学意义(P＜0.001).而不同浓度铜离子组与对照组没有明显差异.转录组测序方法对银离子作用前后大肠杆菌基因表达水平进行对比分析,通过GO功能和KEGG通路富集发现,差异表达基因与甲基半乳糖苷转运(methylgalactoside transport)、麦芽糖转运(maltose transport)、磷酸烯醇式丙酮酸-甘油磷酸转移酶活性(phosphoenolpyruvate-glycerone phosphotransferase activity)和甘油激酶活性(glycerone kinase activity)等功能有关以及涉及氨基酸糖和核苷酸糖代谢(amino sugar and nucleotide sugar metabolism)、鞭毛组装(flagellar assembly)、阳离子抗菌肽(CAMP)耐药性[cationicantimicrobial peptide(CAMP)resistance]、果糖和甘露糖代谢和磷酸糖转移酶系统[phosphotransferase system(PTS)]等代谢通路.通过蛋白互作网络筛选出前12个枢纽基因(ptsG、mnalE、lamB、lacZ、malK、basR、ais、ugd、nagE、metN、malQ 和 malF).[结论]一定浓度银离子可以抑制整合子整合耐药基因盒.铜离子对整合频率影响不明显,因此不是所有具有杀菌性的金属离子都能对整合频率产生影响.银离子抑制细菌整合子捕获耐药基因盒可能是通过影响麦芽糖转运和碳分解代谢来调控.然而,碳分解代谢和麦芽糖转运过程很复杂,需要进一步研究.目前尚无细菌整合子转录组学相关研究,这为解决细菌耐药性问题提供了一个新的途径.

目的:探讨G蛋白偶联受体183(G protein-coupled receptor 183,GPR183)在喉鳞状细胞癌(以下简称喉鳞癌)中的表达及其对喉鳞癌细胞增殖的影响.方法:利用免疫组织化学方法检测42例喉鳞癌组织和5例癌旁组织中GPR183的表达,并分析GPR183表达与患者临床病理参数的关系.采用定量聚合酶链式反应(quantitative reverse transcription quantitative polymerase chain reaction,qRT-PCR)检测正常人胚肾细胞HEK-293T和喉鳞癌FD-LSC-1和AMC-HN-8中GPR183表达水平.将GPR183小干扰RNA(small interfering RNA,siRNA)转入喉鳞癌细胞株FD-LSC-1、AMC-HN-8后,采用qRT-PCR检测转染效果,CCK-8实验、细胞克隆形成实验检测细胞增殖、流式细胞术检测细胞周期.并进行体内实验,以评估GPR183在体内对喉鳞癌生长的影响.结果:GPR183表达水平在喉鳞癌中显著上调,喉鳞癌患者组织中GPR183表达水平与临床T分期(P<0.001)、淋巴结转移(P=0.002)相关;下调喉鳞癌细胞中GPR183表达水平,显著抑制喉鳞癌细胞在体外的增殖能力,并诱导细胞周期阻滞于G1期;体内实验结果表明敲降GPR183延缓了喉鳞癌生长.结论:GPR183在喉鳞癌中呈高表达,下调GPR183能够抑制喉鳞状细胞癌的增殖.

目的 探讨不同程度烧伤患者血浆游离线粒体DNA(mtDNA)拷贝数变化及在烧伤病情中的作用.方法 根据患者烧伤程度、面积(TBSA)选取重度烧伤患者30例(重度烧伤组)及中度烧伤患者30例(中度烧伤组),纳入同期30名健康体检者(正常对照组).采集所有参试者入院第1、8～11、18～21天的血浆标本.所有血浆标本经试剂盒提取DNA,通过实时荧光定量PCR法检测血浆游离mtDNA拷贝数.分析mtDNA拷贝数与急性生理学和慢性健康状况Ⅱ(APACHE Ⅱ)、多器官功能障碍综合征评分(MODS)、TBSA、Ⅲ.烧伤面积、烧伤指数及生化指标间相关性,并将上述指标进行Logistic回归分析.结果 重度烧伤组入院第1天血浆游离mtDNA拷贝数高于中度烧伤组和正常对照组,重度烧伤组血浆游离mtDNA拷贝数在第8～11天到达峰值之后降低;mtDNA拷贝数与烧伤总面积、Ⅲ°烧伤面积、烧伤指数、APACHE Ⅱ评分、MODS呈正相关(P＜0.05),与生化指标天冬氨酸氨基转移酶、谷丙氨酸氨基转移酶、总胆红素、降钙素原、白细胞呈正相关(P＜0.01),与总蛋白、白蛋白及前白蛋白呈负相关(P＜0.01);经Logistic回归分析显示,患者APACHE Ⅱ评分和住院当天mtDNA拷贝数为烧伤严重程度的独立危险因素(P＜0.05);mtDNA拷贝数在诊断烧伤程度的ROC曲线下面积为0.904,敏感度为0.833,特异性为0.867.结论 烧伤患者血浆游离mtDNA拷贝数与烧伤程度和多项生化指标具有相关性,为临床判断烧伤程度提供依据.

Objective:To analyze the effects of silver ion on the integration frequency of the class 1 integron in Escherichia coli ( E. coli) BL21(DE3) host. Methods:Two recombinant plasmids, pUCINT and pACINAD, were successively transformed into E. coli BL21 (DE3) to construct HS2 strains. Three experimental groups were set up using 0.3 μg/ml, 0.6 μg/ml and 0.8 μg/ml silver ion LB liquid medium, while control group used common LB liquid medium. Silver ion was supplied by silver nitrate and HS2 strains were cultured at 37℃ for 24 h. The copy number of cointegrates and the total copy number of integrons in each group were detected by real-time polymerase chain reaction (qPCR), and the ratio of them was the integration frequency. Changes in the integration frequency were analyzed by three independent phenotypic screening method and the protein expression in HS2 strains was analyzed by mass spectrometry. Results:The integration frequency in HS2 strains in the control group and three experimental groups (0.3 μg/ml, 0.6 μg/ml and 0.8 μg/ml silver ion) was 1.79×10 -5 (1.44×10 -5, 3.13×10 -5), 2.07×10 -5 (1.49×10 -5, 2.67×10 -5), 2.25×10 -6 (1.47×10 -6, 4.54×10 -6) and 1.69×10 -6 (0.22×10 -6, 3.08×10 -6), respectively. The integration frequency in the 0.6 μg/ml and 0.8 μg/ml silver ion groups was significantly lower than that in the control group ( P<0.01), but there was no significant difference between the 0.3 μg/ml silver ion group and the control group. Results of three independent phenotypic screening method were consistent with those obtained by qPCR. Mass spectrometry analysis showed that there were differences in protein expression in HS2 strains between the control group and the experimental groups. Conclusions:Silver ion at a certain concentration had an inhibitory effect on the frequency of drug resistance gene cassette captured by bacterial integron.

研究开发了基于智能手机的便携式96微孔板检测技术与仪器,并利用所开发仪器实现了人血清白蛋白(HSA)的高通量快速定量检测.该文首次通过设计大孔径聚焦透镜和均匀面光源,利用智能手机实现了对通用96微孔板的全范围准确成像,结合开发的手机应用程序可实现对检测样本的自动分析.所开发分析仪对HSA标准样品的检测结果与酶标仪检测结果具有高度的线性关系,相关系数为0.989 3,证明其具有良好的准确性与可靠性.开发的分析仪的检测范围为21.45 ～60.06 g/L,对病人血清样品中HSA的检测结果与医院使用的生化分析仪检测结果一致,证明分析仪可用于临床中HSA的检测.该仪器具有成本低和体积小的特点,可推广至经济不发达地区、基层医疗机构和普通用户家中,对疾病的现场快速诊断具有重要意义.

目的 建立一套临床化学检测结果 的自动审核规则,运用计算机系统实现检测结果 的自动审核确认.方法 按照美国临床实验室标准化委员会颁布的AUTO10-A临床实验室检验结果 的自动审核指南,结合患者的基本信息、各检测项目的医学决定水平、检测项目在本实验室的方法 学性能、检验项目之间的关系以及实验室的质量控制水平,设定临床化学审核规则,运用Data Manager 2中间件系统对临床化学检测结果 进行审核.结果 我们为53项生化检测项目建立了540条自动审核规则,对16593份常规化学样本进行285415项测试,结果 显示,自动审核通过的样本为14289份,其中门诊样本自动审核通过率为97％,住院样本自动审核通过率为80％,总的自动审核通过率为86％.报告周转时间,从183 min降至131 min,缩短28.4％.结论 制定合理有效的临床化学审核规则,利用DM2软件的自动审核功能,能够充分发挥全自动流水线的使用效率,缩短TAT,提高临床检验分析后的质量控制水平.

目的 探讨血浆游离线粒体DNA(mitochondrial DNA,mtDNA)拷贝数在急性胰腺炎(acute pancreatitis,AP)患者诊断、预后及病情评估中的应用价值.方法 分别收集40例健康体检者,20例轻度急性胰腺炎(mild acute pancreatitis,MAP)和20例中重度急性胰腺炎(moderate-severe acute pancreatitis,M-SAP)患者标本以及6例急性胰腺炎患者的住院第1、3、7天标本,应用实时荧光定量聚合酶链反应(polymerase chain reaction,PCR)测定所有标本血浆游离mtDNA拷贝数,比较实验组和对照组一般临床指标及血浆游离mtDNA拷贝数,并分析血浆游离mtDNA拷贝数与临床常用指标以及临床评分的相关性,动态监测AP患者住院后血浆游离mtDNA拷贝数变化,受试者工作特征(ROC)曲线评估血浆游离mtDNA对AP的诊断价值.结果 健康对照组、MAP组和M-SAP组血浆游离mtDNA拷贝数分别为5.0×104(3.1×104)、1.2×105(1.6×105)和7.8×105(5.0×105)copies/mL,MAP组明显高于健康对照组,M-SAP组明显高于MAP组,三组间差异均有统计学意义(P＜0.05).AP患者血浆游离mtDNA拷贝数与白细胞(WBC)、C-反应蛋白(CRP)、降钙素原(PCT)、甘油三酯(TG)、病情严重程度(Ranson)评分及急性生理学与慢性健康状况评估系统Ⅱ(APACHE Ⅱ)评分呈正相关(r=0.453、0.539、0.562、0.513、0.653、0.869,P ＜0.05).AP患者在住院治疗后病情好转,血浆游离mtDNA拷贝数先上升后下降.ROC曲线显示,以8.5×104 copies/mL为截断值,血浆游离mtDNA诊断AP的敏感度为90％,特异度为97.5％,ROC曲线下面积为0.989(95％ CI0.97～1.00).结论 AP患者血浆游离mtDNA水平升高,血浆游离mtDNA拷贝数有助于诊断AP,也可作为预测AP病情严重程度及预后判断的一种标志物.

目的 探讨天门冬氨酸氨基转移酶(AST)参考方法的不同测定模式对测量结果精密度的影响,为提高AST测定结果的稳定性提供依据.方法 依据读点时间周期及延迟期混匀时间设计A～I共9种不同测定模式,检测时间段均选取延迟反应时间90 s后连续读点180 s,依据国际临床化学与检验医学联合会(IFCC)推荐的AST参考测量程序建立AST改良参考方法,对样本进行测定,每种模式每天重复测定2次,连续测定20 d,分别计算不同测定模式下的AST测量结果总不精密度.结果 A～I共9种不同测定模式的测量总不精密度分别为0.80％、0.69％、1.31％、1.21％、0.79％％、1.55％、1.30％、0.74％、1.21％.结论 测定模式的差异对AST测定结果有明显影响,读点时间周期为15s、延迟期混匀时间为45 s的测定模式B的AST测量精密度最好.

目的 探讨天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)的参考方法测定值与室间质评(EQA)公议靶值二者的相关性,为EQA公议靶值在评价临床实验室性能中的正确性提供依据.方法 采用AST和ALT参考方法分别对2017年山西省临床检验中心的10个样本及2019年卫生部临床检验中心(NCCL)的5个样本的AST和ALT分别进行测定,将所得值与各自EQA公议靶值进行相关分析、偏倚评估,并根据美国临床实验室改进修正案'88(CLIA'88)规定的1/2允许总误差(TEa)为标准,判断结果是否通过.结果 AST和ALT的EQA公议靶值与参考方法测定值均具有良好的相关性,2017年和2019年室间质评样本的AST、ALT项目的回归方程分别为Y AST2017=1.0106X+1.1078,R2=0.9988;Y ALT2017=1.0602X-0.6117,R2=0.9988;Y AST2019=1.0219X-0.7828,R2=0.9999;Y ALT2019=1.0224X-0.6823,R2=0.9991.所有批号的相对偏倚均在CLIA'88规定的1/2 TEa内.结论 EQA公议靶值现阶段在一定程度上可用于我国EQA计划.

目的 比较Sysmex UF-1000i和朗迈UriSed尿沉渣分析仪检测尿液有形成分结果的一致性,并与标准人工镜检进行比较,评价两种仪器检测尿液有形成分的性能差异.方法 随机选取山西医科大学第六医院住院患者的尿液标本192例,分别用朗迈UriSed和Sysmex UF-1000i尿液分析仪进行检测,同时用显微镜对192例标本进行观察计数红细胞、白细胞、管型,以人工镜检结果为标准,对两种仪器检测结果进行比较.结果 Sysmex UF-1000i、朗迈UriSed两种尿沉渣分析仪检测尿液中红细胞与人工镜检符合率为88.54％、85.42％,灵敏度为82.50％、85.00％,特异度为90.13％、85.53％;白细胞与人工镜检符合率为86.46％、90.10％,灵敏度为85.87％、96.74％,特异度为96.74％、87.23％;管型与人工镜检符合率为86.46％、83.85％,灵敏度为64.29％、78.57％,特异度为90.24％、84.76％.Sysmex UF-1000i和朗迈UriSed尿沉渣分析仪检测红细胞、白细胞、上皮细胞、管型、细菌、结晶的相关系数分别为0.961、0.905、0.923、0.466、0.517、0.680(P＜0.001).结论 Sysmex UF-1000i与朗迈UriSed全自动尿沉渣分析仪检测有形成分准确性较高,Sysmex UF-1000i特异度较高,朗迈UriS ed灵敏度较高,两种仪器有很好的检测相关性,均可用于临床,配合使用效果更好.

目的 评价联合检测妊娠相关蛋白A(PAPP-A)、同型半胱氨酸(HCY)、超敏C反应蛋白(hsCRP)和补体C1q(C1q)水平在子痫前期孕妇患者中的早期诊断价值.方法 采用前瞻性的研究方法,选取2013年4月至2015年2月在山西医科大学第一医院门诊行常规产前检查并在我院分娩的孕妇共117例,根据随访结果将其分为健康对照组、重度子痫前期组和慢性高血压合并子痫前期组.采用酶联免疫吸附试验定量法检测血清中PAPP-A水平、酶循环法检测HCY水平、免疫比浊法检测hsCRP和C1q,对血清指标和部分妊娠结局的相关性进行分析.结果 ①117例孕妇中未发生妊娠期高血压疾病42例(健康对照组),重度子痫前期56例(病例A组),慢性高血压合并子痫前期19例(病例B组).②使用SPSS 22.0软件对数据进行分析,数据不符合正态分布,采用M(QR)进行统计描述,健康对照组孕妇血清中PAPP-A值、HCY值、hsCRP值和C1q值分别是1504(4027)ng/ml、6.80(3.25)μmol/L、3(2.70)mg/L、194(26)mg/L;重度子痫前期组分别是1764(4167)ng/ml、11.70(6.60)μmol/L、5.90(4.24)mg/L、191(40.50)mg/L;慢性高血压合并子痫前期组分别是1715(5047)ng/ml、9.60(8.85)μmol/L、5.98(6.43)mg/L、207(52)mg/L;健康对照组产妇血清中PAPP-A值、HCY值hsCRP值和C1q分别是11.16(22.32)ng/ml、9.60(4.45)μmol/L、1.56(2.47)mg/L和206(38.50)mg/L;病例A组产后分别是7.56(26.91)ng/ml、10.60(6)μmol/L、1.99(2.78)mg/L和206(41)mg/L;病例B组产后分别是9.36(20.52)ng/ml、9.70(5.95)μmol/L、2.75(3.01)mg/L和221(57)mg/L.③经配对计量资料比较的Wilcoxon秩和检验,健康组PAPP-A、HCY、hsCRP和C1q产前产后比较,差异有统计学意义(P<0.05);病例A组PAPP-A、HCY、hsCRP和C1q产前产后比较,差异有统计学意义(P<0.05);病例B组PAPP-A产前产后比较,差异有统计学意义(P<0.05),HCY、hsCRP和C1q产前产后比较,差异均无统计学意义(P>0.05).④经多个独立样本两两比较的Mann-Whitney U检验,3组孕妇血清PAPP-A分别两两比较,差异均无统计学意义(P>0.05);3组孕妇血清HCY分别两两比较,健康组与病例A组、正常组与病例B组,差异均有统计学意义(P<0.05),病例A组与病例B组,差异均无统计学意义(P>0.05);3组孕妇血清hsCRP分别两两比较,健康组与病例A组、健康组与病例B组,差异均有统计学意义(P<0.05),病例A组与病例B组,差异均无统计学意义(P>0.05);3组孕妇血清C1q分别两两比较,差异均无统计学意义(P>0.05).3组产妇血清PAPP-A、HCY、hsCRP和C1q分别两两比较,差异均无统计学意义(P>0.05).结论PAPP-A、HCY、hsCRP和C1q对子痫前期的发病有一定的预测价值.