Picornavirus hepatitis A virus (HAV) is a common cause of hepatitis worldwide. It is spread primarily through contaminated food and water or person-to-person contact. HAV I has been identified as the most common type of human HAV infection. Here, we have developed a cell-free toehold switch sensor for HAV I detection. We screened 10 suitable toehold switch sequences using NUPACK software, and the VP1 gene was used as the target gene. The optimal toehold switch sequence was selected by in vivo expression. The best toehold switch concentration was further found to be 20 nM in a cell-free system. 5 nM trigger RNA activated the toehold switch to generate visible green fluorescence. The minimum detection concentration decreased to 1 pM once combined with NASBA. HAV I trigger RNA could be detected accurately with excellent specificity. In addition, the cell-free toehold switch sensor was verified in HAV I entities. The successful construction of the cell-free toehold switch sensor provided a convenient, rapid, and accurate method for HAV I on-site detection, especially in developing countries, without the involvement of expensive facilities and additional professional operators.

目的:提高对新型冠状病毒肺炎的认识和鉴别.方法:选取2020年2月至2020年3月确诊的170例新型冠状病毒肺炎患者作为研究对象,分析其主要临床特征,并与10例肺炎链球菌肺炎、10例腺病毒肺炎患者的临床特点进行比较.结果:170例患者中有发热160例(94.1％)、干咳109例(64.1％)、咳痰47例(27.7％)、乏力130例(76.5％)、胸闷气短109例(64.1％)、咽痛67例(39.4％)、纳差100例(58.8％)、肌肉酸痛56例(32.9％).出现并发症包括呼吸衰竭44例(25.9％)、休克8例(4.7％)、心力衰竭17例(10％)、死亡4例.普通型患者胸部CT主要表现为两肺多发斑片状影及间质改变,以肺外带明显.重型和危重型患者胸部CT主要表现为双肺多发磨玻璃影、浸润影,范围大,可出现肺实变.肺炎链球菌肺炎与新型冠状病毒肺炎比较,白细胞计数(WBC)、C反应蛋白(CRP)、降钙素原(PCT)明显升高,敏感抗生素治疗有效;腺病毒肺炎与新型冠状肺炎比较,并发症发生率更高,且用丙种球蛋白和全身激素治疗的比例更高.结论:新型冠状病毒肺炎传染性强,危害大,临床表现缺乏特异性,需与肺炎链球菌肺炎、腺病毒肺炎鉴别,避免误诊误治.

目的 探讨血清清蛋白与C反应蛋白对新型冠状病毒肺炎患者预后的预测价值.方法 分析武汉火神山医院2020年2月3日—2020年3月10日收治的139例新型冠状病毒肺炎的临床特征,并确定纳入病例的诊断标准及临床分型.分析不同分型患者血清清蛋白、CRP水平的差异.通过受试者工作特征(ROC)曲线评价血清清蛋白、CRP对患者病情预后的预测价值.结果 139例新型冠状病毒肺炎患者平均(57.3±12.7)岁,起病以发热、咳嗽、乏力为主.其中,普通型97例、重型35例、危重型7例.危重型[(26.3±1.65)mg/L]及重型患者[(32.0±2.52)mg/L]血清清蛋白水平显著低于普通型[(39.2±3.13)mg/L],差异有统计学意义(P<0.05);危重型与重型患者相比,血清清蛋白水平明显减低,差异有统计学意义(P<0.05).危重型[(102.42±15.36)mg/L]及重型患者[(50.52±6.57)mg/L]CRP水平显著高于普通型[(12.56±3.31)mg/L],差异有统计学意义(P<0.05);危重型患者CRP水平明显高于重型患者,差异有统计学意义(P<0.05).相关性分析提示血清清蛋白与C反应蛋白水平呈负相关(r=-0.618,P<0.05).血清清蛋白以35.4 mg/L为截点值,其判断重型、危重型的ROC曲线下面积及其95％CI:0.923(0.879,0.967),约登指数为0.797;CRP以18.8 mg/L为截点值,其判断重型、危重型的ROC曲线下面积及其95％CI:0.954(0.917,0.981),约登指数为0.841.结论 新型冠状病毒肺炎患者大多有血清清蛋白降低、C反应蛋白升高,重型和危重型患者显著.两者联合检测可评估新型冠状病毒肺炎患者的严重程度及预后.

目的 分析新型冠状病毒肺炎(COVID-19)患者的临床特征,研究血清白蛋白、血清C反应蛋白水平的变化与其临床严重程度的相关性,以及评估临床预后的价值.方法 根据国家卫生健康委员会发布的《新型冠状病毒肺炎诊疗方案(试行第七版)》,确定纳入病例的诊断标准和临床分型,分析武汉火神山医院2020年2月3日至3月10日收治的139例新型冠状病毒肺炎患者的临床特征,血清白蛋白、血清C反应蛋白水平在不同分型患者之间以及30 d内死亡与生存患者之间的差异和2项血清学指标的关联.探讨新型冠状病毒肺炎患者进展为重型甚至危重型的危险因素.结果 139例新型冠状病毒肺炎患者平均年龄(57.3±12.7)岁,起病以发热、咳嗽、乏力为主,33例有一种以上基础疾病.危重型7例(5.0%),死亡5例(3.8%).危重型及重型患者血清白蛋白水平显著低于普通型,差异有统计学意义(P＜0.05);危重型与重型患者相比,血清白蛋白水平明显减低,差异有统计学意义(P＜0.05).危重型及重型患者C反应蛋白水平显著高于普通型,差异有统计学意义(P＜0.05);危重型患者血清C反应蛋白水平明显高于重型患者,差异有统计学意义(P＜0.05).入院30 d内死亡患者血清白蛋白水平显著低于生存患者,血清C反应蛋白水平显著高于生存患者,差异有统计学意义(P＜0.05).相关性分析提示,血清白蛋白与血清C反应蛋白水平呈负相关(r=-0.737,P＜0.01).低血清白蛋白、高血清C反应蛋白为COVID-19患者进展为重型或危重型的危险因素.结论 COVID-19患者大多有血清白蛋白降低、血清C反应蛋白升高,重型和危重型患者显著,两者联合检测可评估COVID-19患者的严重程度及预后.

目的 分析老年新型冠状病毒肺炎(COVID-19)患者的临床特征,探讨其护理策略,并比较不同分型患者的差异.方法 回顾性分析2020年2月11日至2020年3月16日武汉火神山医院感染四科二病区收治的34例老年COVID-19患者的临床资料,其中普通型20例(普通组),重型/危重型14例(重/危重组).结果 34例患者中合并基础疾病者20例(58.82％);主要临床表现:乏力(34/34,100.0％)、咳嗽(28/34,82.3％)、发热(24/34,70.6％)、全身酸痛(12/34,35.3％)、气喘(8/34,23.5％)、胸闷(5/34,14.7％),其中重/危重组发热、全身酸痛、气喘和胸闷的比例高于普通组(P＜0.05);普通组呼吸频率低于重/危重组[(16±3)次·min-1比(34±4)次·min-1,t=6.240、P=0.003].实验室指标:C反应蛋白(CRP,22/34、64.7％)、D-二聚体(22/34,64.7％)、乳酸脱氢酶(LDH,16/34、47.0％)升高、淋巴细胞计数(L,14/34、41.2％)降低,其中重/危重组CRP、D-二聚体及LDH升高的比例高于普通组(均P＜0.05),L降低的比例高于普通组(P＜0.05);普通组静息状态下指血氧饱和度高于重/危重组[(96.4±2.2)％比(90.2±2.7)％,t=3.038、P=0.037].普通组治愈率高于重/危重组(100％和71.4％,x2 =6.5、P =0.01),且住院时间短于重/危重组[(12.4±5.5)d比(19.1±8.6)d,t=2.80、P=0.009)].护理措施主要针对患者生命体征、氧疗、药物治疗、营养及心理状态进行干预.结论 老年COVID-19患者多合并基础疾病,临床表现多见乏力、发热和咳嗽,实验指标多见L降低,CRP、D-二聚体和LDH升高.普通型患者治疗以抗病毒、氧疗及基础疾病的控制为主,注意患者饮食,心理干预重在倾听;重型/危重型患者加用高流量给氧、无创或有创辅助呼吸、激素、干细胞输注,重视营养治疗,心理干预重在鼓励与支持.

Background A worldwide outbreak of coronavirus disease 2019 (COVID-19) has drawn global attention. However, up to now, no standard and effective therapy are available. Case presentation A 62-year-old man with a history of hypertension and diabetes was diagnosed with COVID-19 pneumonia. He suffered from obvious shortness of breath and severe hyoxemia. Normal treatments like supportive therapy and antiviral drugs didn’t seem to improve his conditions. Then, he was given tocilizumab and human umbilical cord mesenchymal stem cells. After that, his respiratory symptoms and lung infectious lesions gradually subsided, and he was successfully discharged eventually. Conclusions For critically ill COVID-19 patients, immunological treatment like tocilizumab human umbilical cord mesenchymal stem cells should be considered.

目的 探讨新型冠状病毒肺炎(COVID?19)核酸检查转为阴性超过30 d患者的临床特征.方法 分析2020年2月10日至2020年4月4日火神山医院感染四科二病区收治的由外院确诊转入的170例患者中21例核酸检查反复阳性、持续阳性超过30 d患者的临床资料.结果 ①21例中有17例为中老年患者(45～84岁),15例有慢性基础性疾病,21例发病早期均有不同程度的发热、咳嗽症状,部分轻度活动后气促,均诊断为普通型;②2例血白细胞计数减少,3例淋巴细胞降低,4例C?反应蛋白高,21例肺CT示肺浸润范围1.50％～20.00％,平均范围为3.92％;③予氧疗,根据病情予控制血压、调整血糖等基础治疗,给予足疗程阿比多尔、胸腺肽、中药等治疗,抗病毒7d不转阴者再调整抗病毒药物,予口服磷酸氯喹、法维拉韦、输恢复期血浆治疗;④从第一次核酸检测阳性到核酸转阴30～40 d的4例,41～60 d的13例,>60 d 4例.结论 COVID?19核酸检测反复阳性、持续阳性时间超过30 d患者多见于中老年,多合并慢性基础病,一般抗病毒治疗及免疫治疗核酸转阴效果不理想,需多种抗病毒手段干预.此类患者核酸持续阳性时间长,给防控带来巨大的困难.

Objective:To determine the effects of zoledronate plus interlukin 2 (IL-2) on the proliferation of peripheral blood mononuclear cells (PBMCs) of patients with gastric cancer in vitro.Methods:PBMCs were isolated from 30 healthy doners and 30 patients with gastric cancer respectively,and then stimulated with zoledronate plus IL-2 in vitro.Flow cytometry (FCM) was used to evaluate the γδT cell subpopulation of PBMCs and detect the expression of CD69 in γδT cells.Results:The percentage and absolute number of γδT cells from PBMCs were both lower in patients with gastric cancer than those of the control group (P＜0.05).The combination of zoledronate and interlukin 2 could significantly increase the percentage and absolute number ofγδT cells from PBMCs in patients with gastric cancer and healthy control group (P＜0.05).Conclusion:Zoledronate and IL-2 could obviously stimulate the proliferation of γδT cells from PBMCs to further kill the cancer cells.

ABSTRACT:Objective To observe the clinical effect of mesalazine combined with probiotics on ulcerative colitis.Methods A total of 62 patients with mild to moderate ulcerative colitis were randomly treated with enteric-coated mesalazine tablets alone(control group,n=31)or in combi-nation with Saccharomyces boulardii (combined treatment group,n = 31 ).Clinical efficacy was compared between the two groups after treatment for 8 weeks.Results Compared with control group,combined treatment with mesalazine and probiotics induced a significant increase in the en-doscopic score difference before and after treatment(1.47±0.42 vs 0.86±0.33,P <0.05).In ad-dition,the total effective rate in combined treatment group(90.3%,28 cases)was significantly higher than that in control group(64.5%,20 cases)(P <0.05).Conclusion Mesalazine combined with probiotics is safer and more effective than mesalazine alone for treating mild to moderate ul-cerative colitis.

553例中国人民解放军第九四医院及中国人民解放军第八一医院的胆总管结石患者,随机分为两组,296例行十二指肠乳头切开取石术后再行胆管引流术(EST+ENBD或EST+ERBD),257例单纯行十二指肠乳头切开术(EST),对近期出现胆管炎发生率进行对照性研究.胆管炎发生率EST+ENBD或EST+ERBD组为0.00％,EST组为2.34％,两组比较差异有显著性(P＜0.05).十二指肠乳头切开取石术后常规行胆管引流术用于治疗胆总管结石,减少或避免了胆管炎发生.

ABSTRACT:Objective To study the effects of small endoscopic sphincterotomy(sEST)plus en-doscopic papillary balloon dilation(EPBD)on recent complications of choledocholithiasis(duode-nal papilla hemorrhage and acute pancreatitis).Methods A total of 355 choledocholithiasis pa-tients from No.94 and No.81 Hospitals of PLA were randomly divided into endoscopic sphincter-otomy(EST)group(n=257)and sEST+EPBD group(n=98).The incidence of recent complica-tions was compared between the two groups.Results The incidence of duodenal papilla hemor-rhage in sEST+EPBD group was significantly lower than that in EST group(1.02% vs 6.61%, P <0.05).However,no significant difference in the incidence of acute pancreatitis was found be-tween the two groups(6.12% vs 6.23%,P >0.05).Conclusion The combination of sEST and EPBD for choledocholithiasis can decrease the incidence of duodenal papilla hemorrhage,partly preserve the function of duodenal papilla sphincter,and induce an incidence of acute pancreatitis that is equivalent to EST.

目的 评价内镜套扎术联合β-受体阻滞剂治疗食管静脉曲张破裂出血的安全性和有效性.方法 分析63例食管静脉曲张破裂出血以内镜套扎术联合β-受体阻滞剂治疗及随访情况,总结治疗成功率、并发症及再出血发生率等.结果 内镜套扎术治疗63例食管静脉曲张破裂出血止血成功率100％(63/63),早期再出血率1.6％(1/63),早期并发症发生率7.9％ (5/63).联合口服β-受体阻滞剂继续治疗,63例随访6～18个月,平均12个月,再出血发生率为6.3％(4/63),6个月生存率为98.4％(62/63).结论 采用内镜套扎术联合口服β-受体阻滞剂治疗食管静脉曲张破裂出血简便安全,止血效果肯定,值得推广应用.

Objective To examine the levels of plasma tissue factor pathway inhibitor (TFPI) in patients with acute pancreatitis (AP) to assess the clinical value of diagnosis for severe acute pancreatitis ( SAP).Methods Sixty-eight patients were divided into mild acute pancreatitis (MAP)group (n=36) and SAP group (n=32), and twenty volunteers were chosen into normal group ( n=20 ) .Clinical data of these patients were collected, including APACHEⅡscore and Ranson score.Plasma levels of TFPI were measured by ELISA.Results The plasma levels of TFPI in SAP group, MAP group and control group were (4274.25 ±639.83),(3026.81 ±465.76) and (2468.73 ±262.39)pg/ml, respectively(P<0.05).There were significant positive correlations between TFPI and WBC, AST, ALT, TBIL, Cr, PT, APTT, PCT, APACHEⅡscore and Ranson score (P<0.05).The area under the curve (AUC) of TFPI for SAP was 0.902(95%CI=0.845 -0.959, P<0.05 ) .The cutoff value was 4028.83 pg/ml for plasma TFPI with a sensitivity of 87% and a specificity of 78%.Conclusion Plasma levels of TFPI in patients with SAP are significantly increased, which maybe help diagnose SAP.

患者，女，56岁。因解柏油样便、呕血伴头晕乏力1 d入院。患者1d前无诱因出现上腹不适，解柏油样便2次，每次量约200 g，入院前4 h出现恶心，继而呕吐鲜血1次，量约500 ml，伴头晕乏力。既往有高血压病史。入院时查体：T 36．8℃，P 96次／min，BP 112／78 mm Hg。神志清楚，贫血貌，心肺检查未见明显异常。腹平软，无明显压痛，肝脾肋下未及，腹水征阴性，肠鸣音活跃。实验室检查：血WBC 6．8×109／L，Hb 82 g／L。肝肾功能未见明显异常。心电图：窦性心律，T波轻度改变。腹部彩色超声示：（1）胆囊壁毛糙，肝脏回声增粗、增强；（2）右肾小结石，左肾囊肿。入院后即给予补液扩容、制酸止血等处理，患者病情稳定，血压平稳。入院2d后再次出现呕血2次，总量约600 ml。脉搏增快，血压下降。经积极补充血容量抗休克等处理，患者生命体征平稳后行急诊胃镜检查示：食管下段、胃底体部黏膜见红色血液覆盖，用冰冻去甲肾上腺素盐水反复冲洗，于距贲门约5 cm处见一局灶性黏膜缺损及搏动性射血点。诊断胃恒径动脉破裂出血。在内镜下给予COOK公司六连环套扎器行套扎治疗：先将套扎帽小心对准并完全接触病灶出血点，然后负压吸引至视野变成红色，维持2～3s后释放套扎环，一次套扎成功。吸干胃腔潴留积血后反复观察，未见活动性出血。胃窦及十二指肠球部亦未见溃疡等出血病灶。术后常规制酸止血治疗1周后患者大便转黄出院。

目的观察铝镁加混悬液联合枳术宽中胶囊治疗胆汁反流性残胃炎的疗效。方法将120例胆汁反流性残胃炎患者用随机数字表法随机分为治疗组和对照组,每组60例。治疗组给予铝镁加混悬液每次1.5 g,3次/d口服;枳术宽中胶囊,每次1.29 g,3次/d口服。对照组给予兰索拉唑胶囊30 mg,每晚1次口服,多潘立酮片10 mg,3次/d口服。两组在治疗6周后比较临床疗效。结果两组患者治疗后临床症状评定比较,治疗组总有效率90.0%,对照组总有效率65.0%,差异有统计学意义(P<0.01)。电子胃镜下总有效率治疗组为78.3%,对照组为46.7%,两组比较差异有统计学意义(P<0.01)。结论铝镁加混悬液联合枳术宽中胶囊治疗胆汁反流性残胃炎疗效确切。

Objective To investigate the protective effects of p38MAPK inhibitor SB203580 on acute necrotizing pancreatitis associated lung injuries in rats.Methods Fifty-four SD male rates were randomly divided into 3 groups,including control group,ANP group,SB203580 group with 18 rats in each group.ANP was induced by intraperitoneal injection of L-arginine solution. Rats in control group were intraperitoneally injected with same amount of saline.Before ANP induction,the rats in SB203580 group received 10 μmol/L SB203580 dissolved by dimethyl sulfoxide at a dose of 5mg/kg weight via intraperitoneal injection.The rats were sacrificed at 3,6,and 12 h after operation,the serum levels of amylase,TNF-α,IL-6 was determined.Pathological changes of pancreas and lung were observed.The wet/dry (W/D) weight ratio of lung and MPO were measured.CINC mRNA of lungs was determined by RT PCR. Expression of phosphated-p38MAPK (p-p38MAPK) protein was evaluated by Western blotting.Results The serum levels of amylase,TNF-α,IL-6and wet/dry (W/D) weight ratio of lung,MPO activity,CINC mRNA and p-p38MAPK protein expression of lungs were (1035 ±73)U/L,(0.94 ±0.16)μg/L,(4.77 ±0.86) μg/L,3.92 ±0.29,(0.39 ±0.02)U/g,0.28 ±0.04,0.09 ±0.04 in control group at 6 h after operation,and the corresponding values were (5848 ±656) U/L,(3.84 ±0.32)μg/L,(103.54 ± 15.32)μg/L,4.97 ±0.47,(1.03 ±0.08) U/g,0.62 ±0.06,0.52 ±0.14 in ANP group,while they were (4259 ±286) U/L,( 1.64 ±0.21 ) μg/L,(76.56 ± 11.46) μg/L,4.32 ±0.34,(0.78 ±0.05)U/g,0.37 ±0.04,0.27 ±0.08 in SB203580 group.The values in ANP group were significantly higher than those in control group,and the values in SB203580 group were significantly lower than those in ANP group,but they were still significantly higher than those in control group ( P ＜ 0.01 ).Conclusions SB203580 may attenuate injury of lung and pancreas in ANP by blocking p38MAPK signal transduction pathway,and decreasing the production of inflammatory cytokines.

Objective To explore the potential role of monocyte chemotactic protein-1 ( MCP-1 ) gene in the pathogenesis of acute lung injury (ALI) in early acute necrotizing pancreatitis (ANP). Methods Forty SD rats were randomly divided into control group, ANP 3, 6, 12 h group with 10 rats in each group according to a number table. ANP was induced by intraperitoneal injection of 15% L-arginine solution at a dose of 2.0 mg/g body weight. Pathological changes of pancreases and lungs were observed. Lung wet/dry weight ratio was measured. Intrapulmonary expression of MCP-1 mRNA was evaluated by RT-PCR. Results After intraperitoneal injection of 15% L-arginine solution, the rat's pancreas presented with bleeding, necrosis comparable with pathological changes of ANP. Pulmonary tissue edema was obvious. At ANP 3, 6, 12 h group, the pathological scores of the lung were 3.75±0.58,5.50 ±0.63,5.86 ±0.54, the wet/dry weight ratios were 4.85 ±0.38,4.97 ± 0.47,5.03 ± 0. 46, the MCP-1 mRNA expressions were 0.36 ± 0.08, 0. 56 ± 0. 15, 0. 72 ± 0.21,which were significantly higher than those in the control group (0.12 ±0.05,4.32 ±0.33,0.21 ±0.05, P＜0.05 or ＜0.01 ). The MCP-1 mRNA expression in lungs was significantly correlated with the degree of lung damage and wet/dry weight ratio of lungs (r=0.75,r=0.89,P＜0.05).Conclusions MCP-1 mRNA expression was up-regulated in the early phase of ANP in the lungs, and it may play an important role in ALI during ANP.

Objective To investigate the changes of extraceUular regulated kinase 1/2 (ERK1/2) phosphorylation and assess the effects of blocking the ERK1/2 phosphorylation on rats with acute necrotizing pancreatitis (ANP). Methods The ANP model was induced by retrograde injection of 5% sodium tanrocholate into the biliary and pancreatic duct. 5 rats were treated as normal control. Other 75 Sprague-Dawley (SD) rots were randomly divided into sham operations(SO) group (n =25), ANP group (n =25) and PD98059 group (n =25). The rats were sacrificed at 15 min, 30 min, 1 h, 3 h and 6 h after ANP induction, the blood and pancreatic sample were taken. Pathological changes of pancreas were observed with light microscope and scored. The serum level of TNF-α and IL-1β was determined by ELISA. MPO activities in pancreas were measured by enzyme chemistry assay. Western blotting was performed to determine the phosphorylations of ERK1/2 in the pancreas homogenates. Results There was no significant pathologic changes in rats of SO group;but significant injuries occurred in ANP group, the pathologic score at 3 h was 9.9 ± 0.4;the extent of injuries attenuated in PD98059 group, the pathologic score at 3 h was 4.0 ± 0.4 (P < 0.05). The serum levels of TNF-α at 3 h in SO, ANP and PD98059 groups were (65.8 ± 20.5) pg/ml, (286.5 ± 50.3) pg/ml, (180.4±32.9)pg/ml, respectively;the serum levels of IL-1β at 3 h in SO, ANP and PD98059groups were (85.8 ± 25.5) pg/ml, (293.8 ± 46.3) pg/ml, (200. 5 ± 33.6) pg/ml, respectively;MPOactivities in pancreas were (0. 19 ± 0.02)U/g, (0.61±0.05)U/g, (0.52±0.03) U/g, and the values in ANP and PD98059 groups were significantly higher than those in SO group, while the values in PD98059 group were significantly lower than those in ANP group (P < 0.01). The expression of ERK1/2 phosphorylation in normal pancreas was 1100 ± 141, the expressions of ERK1/2 phosphorylation in ANP group at 15 min, 30 min were 5300 ± 486, 5621 ± 384, respectively;the expressions began to decrease 1 h later and returned the similar level as SO group at 6 h;the expressions of ERK1/2 phosphorylation in PD98059 group at 15 min, 30 rain were 4200 ± 370, 3600 ± 290, respectively;which were signifieanfly lower than those in ANP group (all P value < 0. 01). Conclusions The ERK1/2 signal transduetion pathway plays an important role in the pathogenesis of ANP. Inhibition of ERK1/2 phosphorylation by PD98059 may decrease the production of IL-1β, TNF-α and pancreatic MPO, attenuate the extent of pancreatic pathologic injuries.

Objective To investigate the effects of rhubarb and mirabilite and compound danshen injection on gastrointestinal dysmotility of severe acute pancreatitis (SAP). Methods Forty-one SAP patients were randomly divided into two groups: Integration group (n=21) were treated with rhubarb and mirabilite and compound danshen injection. Control group (n=20) were treated with conventional therapy. Recovery of gastrointestinal motility of two groups was observed, and the complication rate and hospital stay was recorded.Results The recovery of gastrointestinal motility, first defecation and hospital stay in integration group was (3.1±0.8)d, (3.1±0.8)d, (21.5±2.8 )d, which were significantly shorter than those in control group [(5.2±1.4) d, (4.7±1.3 ) d, (32.1±3.60) d, P ＜ 0. 05 or P ＜ 0.01. Five patients in integration group developed complication, which was significantly lower than 8 patients in control group ( P ＜ 0.01 )]. One patient died and one patient received operation in integration group, and one patient died in control group,without significant difference between the two groups (P ＞ 0.05 ). Conclusions The combination of rhubarb and mirabilite and compound danshen injection in severe acute pancreatitis can promote the recovery of gastrointestinal motility.

急性胰腺炎(acute pancreatitis,AP)是发生于胰腺的炎症性疾病,建立合适的动物模型有助于其研究.目前较多采用逆行胆胰管注射法制备急性坏死性胰腺炎(ANP)模型和腹腔注射雨蛙素制备急性水肿性胰腺炎(AEP)模型.本文采用不同浓度的左旋盐酸精氨酸腹腔注射建立两种AP模型,现报告如下.