To improve the quantitative detection efficiency of transgenic rice,and promote the implementation of transgenic food quantitative identification system,the components of transgenic rice ‘ Bt shanyou 63 ’ were analyzed with droplet digital PCR quantitative detection method.Based on the exogenous inserting sequence of transgenic rice ‘Bt shanyou 63’ and the rice sucrose phosphate synthase (SPS) gene,high-efficiency primer probes were selected,designed and synthesized to amplify the endogenous gene and the strain specific sequence of ‘Bt shanyou 63’ with droplet digital PCR.The result of primer probes optimizing experiment demonstrated that SPS60-F/R/P and ‘Bt shanyou 63’-F/R/P was suitable for quantitatively detecting the components of ‘Bt shanyou 63’ with dual channel ddPCR method.The accuracy verification experiment showed that the measured value was similar to the standard value,and the biases were 0.03,0.15,0.15 and 0.03,respectively.SD of measured value was 0.08-0.48,RSD was 4.86％-15.10％,and they were less than the requirement of European Union,indicating that the accuracy was good.The established detection system of droplet ddPCR method for transgenic rice line ‘Bt 63’ quantification is simple,high efficient and accurate,and it can be used for quantitative analysis of transgenic rice line ‘ Bt 63’ in agricultural products and foods.

An amperometric glucose oxidase (GOx) biosensor based on gold nanoparticles (AuNPs) -reduced graphene oxide (rGO) was fabricated for the detection of glucose in beverages.AuNPs-rGO composite was synthesized by one-pot method with chitosan as reducing and stabilizing agent.It was used as the matrix for GOx immobilization via physical adsorption technique to fabricate the GOx-based biosensor.The biosensor was applied in the amperometric determination of glucose at-0.45 V(vs.Ag/AgCl) in a phosphate buffer (0.1 mol/L,pH 6.0).There was a linear response to glucose in the concentration range of 0.01-0.88 mmol/L,with a sensitivity of 22.54 μA · mmo1-1.L · cm-2 and a detection limit of 1.01 μmol/L.The apparent Michaelis-Menten constant was rather small(0.497 mmol/L).The constructed biosensor was successfully applied in the detection of glucose in beverages.

[目的]对分离自不同产地和品种的蜂蜜中的芽孢杆菌属进行鉴定分型，探索利用蜂蜜中芽孢杆菌特征指纹图谱对蜂蜜产地和品种进行溯源的可行性。[方法] 从44种不同产地和品种蜂蜜中分离出44株芽孢杆菌，3株铜绿假单胞菌。采用基质辅助激光解析-电离飞行时间质谱（MALDITOFMS）条件对分离的芽孢杆菌进行鉴定分型和聚类分析。[结果] 44株芽孢杆菌中鉴定出蜡样芽孢杆菌（B.cereus）31株，短小芽孢杆菌（B.pumilus）5株，枯草芽孢杆菌（B.subtilis）3株，地衣芽孢杆菌（B.licheniformis）2株，炭疽芽孢杆菌（B.anthracis）1株，土壤短芽孢杆菌（Brevibacillus agri.）2株。通过多次重复试验，表明从同一份样品或同一品牌蜂蜜样品中能稳定分离到芽孢杆菌，获得的蛋白指纹图谱具有极好的稳定性。[结论]MALDITOFMS可根据获得的芽孢杆菌蛋白指纹图谱将芽孢杆菌划分为不同类型，可作为一种新的根据蜂蜜中芽孢杆菌的特征指纹图谱进行溯源的方法。

针对红脂大小蠹危害程度不同的3个地区的球孢白僵菌种群,利用ISSR(inter-simple sequence repeat)分子标记分析了各个种群的遗传多样性.从19条引物中筛选出10条多态性高、稳定性好的ISSR引物用于扩增分析.68株球孢白僵菌的Nei's基因多样性(h)为0.2973,Shannon指数(Is)为0.4488.旬邑、宜君、古交3地白僵菌种群间的基因分化系数(GST)为0.0525,基因流(Nm)为9.0255;而来源于土壤、红脂大小蠹、蛀屑和树皮的白僵菌种群间的基因分化系数为0,1449,基因流为2.9508.各球孢白僵菌种群表现出不同的多样性水平,旬邑种群和红脂大小蠹虫种群的遗传多样性相对较高;地理分布种群间的差异不如分离基质种群间的差异明显,地理分布种群间存在明显的基因交流,而分离基质种群间的基因流较低,遗传分化明显.

In order to provide the available reference data for improving the detection efficiency of tobacco disease ,prevent the hazard diseases of tobacco and tobacco products from entering or exi-ting China with import and export trade ,and insure the quality of tobacco and tobacco products for import and export trade ,the occurrence status ,characteristics and pathogens of several main tobacco nematode diseases ,bacterial diseases ,fungal diseases and virus diseases were introduced in this paper .Also ,the research progress on detection methods of tobacco diseases was reviewed sep-arately .

Immunomagnetic?beads?were?used?to?detect?Salmonella?in?dehydrated?garlic?products.?A?large?volume?of?Salmonella?suspension?was?added?with?immunomagnetic?beads?and?high-speed?centrifugation?was?used?instead?of?magnetic?bar?for?bacterial?separation.?The?results?of?bacterial?capture?and?separation?showed?that?immunomagnetic?beads?were?highly?specific?and?sensitive.?The?detection?rates?were?70%?and?100%?for?contamination?levels?of?1?CFU/25?g?and?10?CFU/25?g,?respectively.

The hapten ergonovine was synthesized by Succinic anhydride method.And the hapten was conjugated with carrier proteins with carbodiimide(EDC) method.The coupling ratio of the artificial antigen was determined by Ultraviolet spectrogram(UV) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).The coupling ratio was 12∶1 and 17∶1,respectively.In addition,infrared spectroscopy(IR) identification results further show that the success of artificial antigen synthesis.The antibody with high titer was produced after immuning to New Zealand rabbits.The results showed that the artificial antigen of ergonovine had been synthesized successfully.

To established an import and export food histamine by enzyme-linked immunosorbent fast determination method.According to the current domestic market common Abraxis,r-Biopharm and Neogen three kit,carries on the comparison to choose the optimum kit and enzyme-linked immunosorbent analysis quantitative detection of histamine residual kit for selective(cross reaction) testing,specificity and detection limit testing and samples of import and export food of histamine recovery rate and precision testing.The results showed that choose r-Biopharm kit to test samples of the histamine accuracy is better,which to N-Acyl-histamine cross is 100%,to N-Methyl-histamine,5-Hydroxy-indole-acetic acid,Imidazole acetic acid,L-Histidine,N-Methyl imidazole acetic acid and Serotonin cross reaction are less than 1%.Red wine detection limit(LOD) is 250 mg/kg,milk LOD is 100 mg/kg,cheese LOD is 2.5 mg/kg,fishmeal LOD is 100 mg/kg,the average recovery of sample in both 86.4%~ 95.6%,relative standard deviation 2.7%~3.9%.After five laboratory of recycling verify all have good recovery,by using HPLC corroborate false-positive rate not more than 2.5%.The results indicated that enzyme-linked immunosorbent analysis quantitative method can realize rapid and accurate in food histamine residues screening.

This paper describes an enzyme-linked immunosorbent assay （ELISA） to determine ractopamine in meat and meat products. The proposed method was highly accurate in determining ractopamine and showed a cross-reactivity of less than 1% with all ractopamine analogues investigated. The limits of detection （LODs） for exported canned pork and exported beef were （0.28±0.30） μg/kg and （0.26±0.21） μg/kg, respectively, with an average spike recovery of 82.9%-91.1% （n = 15） and a relative standard deviation of 4.3%-8.7%. Using high performance liquid chromatography （HPLC）, the false posiitve rate of this method was confirmed as less than 2.0%, suggesting that it can enable accurate and rapid determination of ractopamine residues in foods.

Currently cotton and oilseed rape are the two major genetically modified nectar source plants that have obtained permission to be produced in large scale. This study is to set up the standard pollen maps based on the morphological observation of those two pollens under microscope. Thus the honey not containing those pollens can be quickly ruled out through the microscope test. This method can also be applied to identify the honey breed.

The qualitative detection was carried out on genetically modified rice by using real-time fluorescence PCR technology and genetically modified rice in Anhui Province was evaluated.Some advices for strengthening propaganda and management of genetically modified rice were put forward.

The objective of this study was to examine the effect of different intensity of heat stress on sensory properties,blood biochemical parameters and heat shock protein(Hsp) 108 mRNA expression of broilers.Eighty 30-dayage healthy Arbor Acre(AA) broilers were randomly divided into 4 groups,20 broilers for each group.Broilers were heat stressed for 0 h(as a contro1) and for 4 h by rapidly increasing the ambient temperature from(25±1) ℃ to(33±1) ℃,(37±1) ℃ and(41±1) ℃,respectively.The pH,drip loss,color and shear force of broiler breast muscles in every group were investigated.Clinical blood parameter and fluorescence quantitative PCR(FQ-PCR) were used to study the blood biochemical parameters and the expression of heat shock protein108 mRNA in the muscles,hearts and livers of heat-stressed broilers.The results showed that,in comparison with the control group,the pH 30 min,pH 24 h and a* value of broiler breast muscles were significantly decreased and the drip loss,L* and shear force value were clearly improved.The activities of creatine kinase(CK),lactate dehydrogenase(LDH) and malondialdehyde(MDA) were significantly increased with the temperature increasing,the activity of alkaline phosphatase(AKP) was decreased.By FQ-PCR,a decreasing tendency was showed in almost heat shock protein 108 mRNA expression,but the expression of the 33 ℃ group in the broiler hearts was significantly increased,other stressed groups were somewhat decreased.The established FQ-PCR method was qualified after the examination and the heat shock protein could be used as a sensitive indicator of heat stress.

The method of ELISA applied in residue determination of clenbuterol hydroehloride in export meat and products was introduced in this paper. Use clenobuterol hydrochloride kit on export meat and products residual clenobuterol hydrochloride were determined by enzyme standard instrument. The results showed that the cross reaction of clenobuterol hydrochloride is 100%, the detection limit of canned beef and pork（LOD） is 0.026 ug/kg and 0.028 ug/kg, the average recovery of samples is between 83.6% and 91.3%, and relative standard deviation is 3.5%-5.6%, false positives confirmed by HPLC using 2.0%, by using HPLC corroborate false-positive rate not more than 2.0%. The results indicated that enzymelinked immunosorbent analysis quantitative method can realize rapid and accurate in food clenbuterol hydroehloride residues screening.

配体指数级富集系统进化技术(SELEX)是一种体外筛选技术,其筛选的核酸配基具有靶分子范围广、亲和力和特异性高、稳定性好、制备方便等优点。适配子的功能类似于抗体但明显优于抗体,它在微生物检测方面有着广泛的应用前景。

The allergens is the body's allergic reaction occur in the environment.The food allergy has become an extremely serious problem of the fooa safety.This paper introduced that the concept,types,hazards,testing methods,and the management and control issues of the food safety.

Currently cotton and oilseed rape are the two major genetically modified nectar source plants that have obtained permission to be produced in large scale.By extracting cotton and oilseed rape pollens,this study has adopted the technology of breaking the pollen wall,the DNA extraction and purification.The PCR technology was used to establish the rapid qualitative detection methodology for testing the genetically modified ingradients in honey.

Products and damage of important species of Dendroctonus were introduced,the morphology and distribution of all species were summarized.Taxonomic status of D.rhizophagus was clarified,the key to 19 species of Dendroctonus was listed.All of these were used to research,in order to strengthen the quarantine and inspection and protect the production and ecological security for agriculture and forestry.

通过对安徽省出口淡水小龙虾仁中金黄色葡萄球菌污染情况及危害的调查与分析,步解决问题的方案.为企业建立和改进HACCP体系提供参考依据.

此次水平测试是中国实验室国家认可委员会领导,我国首次承办亚太实验室认可合作组织( APLAC)食品微生物学 水平测试,测试五个项目(细菌总数,大肠菌群,粪大肠菌群,沙门氏菌,单核细胞增生李斯特氏菌).在采用国标、行 标同时,配合" 3M"检测试纸," miniVIDAS"全自动细菌鉴定仪等新技术,顺利通过 APLAC水平测试.