DNA甲基化作为表观遗传调控机制,是疾病潜在的诊断标志物及治疗靶点,其可通过参与平滑肌细胞增殖、参与脂质形成、调控炎症反应及参与AS危险因素的形成等作用,影响动脉粥样硬化的进程.DNA甲基化的微观表象可以揭示"天人相应""阴阳自和"等中医经典理论,而中药复方及单体通过调控DNA甲基化可调节特定基因的表达,在稳定动脉斑块、增强抗凝疗效、保护血管内皮细胞等方面发挥作用,延缓及改善动脉粥样硬化的发展.

扩张型心肌病(DCM)是以左室、右室或双心腔扩大和舒缩功能障碍为主要特征的复合型心肌病,具有高发病率、高死亡率、逐渐年轻化等特点.DCM可引起心律失常、严重心力衰竭,甚至猝死,但目前尚缺乏有效的治疗方案,PCM病死率居高不下,是心血管系统疾病一大治疗难题.长链非编码RNA(lncRNAs)不参与或很少参与蛋白质编码,研究发现,lncRNAs在DCM发生发展过程中扮演着重要角色.为寻求更好的治疗方案,目前DCM研究热点转向基因分子水平,探索相关临床诊断标志物和治疗靶点.本文就lncRNAs参与调控DCM作用机制及研究进展进行综述,旨在为DCM的预防和治疗提供新的方向.

Dilated cardiomyopathy (DCM) is a type of composite cardiomyopathy characterized by left ventricular, right ventricular or double ventricular enlargement and diastolic dysfunction caused by genetic and non-genetic causes, with a high incidence, High mortality, gradual rejuvenation, etc., are common types of cardiomyopathy. With the progressive development of the disease, it can cause arrhythmia, severe heart failure, thromboembolism, and even sudden death. Currently, there is no effective treatment plan, and the high mortality rate is a major problem in the treatment of cardiovascular diseases. Long-chain non-coding RNAs (lncRNAs) are a class of RNAs with a molecular mass of more than 200 bt and which are not involved or rarely involved in protein coding. Studies have shown that lncRNAs play an important role in the occurrence and development of DCM. Finding relevant clinical diagnostic markers and therapeutic targets is the current research focus in the field of DCM. This article reviews the mechanism and research progress of lncRNAs in the occurrence and development of DCM, and aims to provide a new direction for the prevention and treatment of DCM.

帕金森病目前尚无特效治疗方法,而针灸治疗帕金森病具有一定优势.就近年来针灸治疗帕金森病的临床研究文献,从帕金森病的中医病因病机、针灸作用机制、针灸治疗帕金森病的研究现状进行归纳论述,并对针灸研究现状及未来研究进行总结与展望.

高血压是遗传和环境因素相互作用的一种复杂性疾病,其发病机制和治疗一直是医药界的研究重点.近来大量研究表明MicroRNA对高血压的发生发展和治疗有重要的作用,包括对血管内皮细胞的功能紊乱、血管平滑肌和肾素血管紧张素醛固酮系统的影响,且miRNA作为高血压病的治疗靶点,通过对miRNA的调控,可以发挥其治疗功能.中医药防治高血压与miRNA调控相结合,可揭示高血压发病机制和治疗新的认识.miRNA无论从诊断和治疗高血压都有巨大的研究价值,是进一步研究高血压的重要方向.

目的 建立肾动脉狭窄性高血压食蟹猴模型,并制定其评价标准.方法 选取7只正常成年雄性食蟹猴,使用丝线及针头狭窄肾动脉,运用两肾一夹(2K1C)法,制作肾动脉狭窄性高血压食蟹猴模型,并对模型进行评价,同时检测血生化相关指标.结果 食蟹猴血压变化:术后1周血压开始升高,4周后达到稳定水平,平均血压为(18.70±1.30)/(10.62±0.91)kPa,食蟹猴术后血压显著高于术前(P<0.05),并且可维持至本实验持续时间8周.食蟹猴血生化变化:尿素、肌酐、谷丙转氨酶、谷草转氨酶术后7 d明显升高,与术前比较差异有统计学意义(P<0.05);总胆固醇与葡萄糖升高不明显,与术前比较差异无统计学意义(P>0.05).造模成功率约为71.43％.结论 运用2K1C法成功建立肾动脉狭窄性高血压食蟹猴模型,该模型经济、操作简单易行、成功率高、稳定性好.

Objective:To compare the differences and advantages of CT angiography (CTA) and color Doppler ultrasound in the imaging evaluation of renal artery of cynomolgus monkey. Methods:Collected nine of the cynomolgus monkey which were accorded with the inclusive criteria,respectively,testing with CTA and color Doppler ultrasound. Two groups of images were an-alyzed and compared,and the diameter of the renal artery was measured. Results:CTA and color Doppler ultrasound images could clearly show the renal artery and its main artery branches. Measurement of left renal artery diameter (CTA:2.83 ±0.49 mm;color Doppler Ultrasound:2.35±0.40 mm),P>0.05 (P=0.053),the difference was not statistically significant. The right renal artery diameter (CTA:2.85±0.56 mm;color Doppler ultrasound:2.37±0.29 mm),P>0.05(P=0.052),the difference was not statisti-cally significant. Conclusion:CTA and color Doppler ultrasound can be used to measure the diameter of the renal artery of the cynomolgus monkey,the difference is not statistically significant. Those two imaging methods are comparable,but each has its advantages and disadvantages.

石墨烯及其衍生物能在生物医学、电子信息学、电磁学、光学和力学等诸多学科领域展现出空前的发展前景，得益于其优异的柔韧性和超强的硬度等力学性能，以及优秀的导热、电性。本文就近年来石墨烯及其衍生物在生物检测、药物载体及其毒性、安全性的研究进展作综述。

高血压是遗传因素和环境因素相互作用而导致正常血压调节机制失衡所导致的疾病,是导致心血管疾病的最危险因素之一[1].由于高血压发病的具体机制尚未完全阐明,近年来对高血压的研究也一直是热点问题.为了更好的研究高血压的发病机制、防治和预后,动物模型的建立是其研究的重要基础.根据造模种属不同,有不同种属的高血压动物模型;根据造模方法的不同,有遗传性、饮食性、应激性、手术性、神经源性、药物性和基因工程性高血压动物模型,本文对不同种属的高血压动物模型和不同方法的高血压动物模型进行了归纳,为高血压疾病的治疗和预后提供参考.

目的 探讨广西地区壮族与汉族高血压病各证型分布规律及危险因素差异.方法 对符合要求的高血压病患者进行回顾性调查研究,应用卡方检验及t检验统计方法分析壮族与汉族高血压病中医证型分布,壮、汉族高血压病中医证型在年龄、性别分布及心血管危险因素差异性.结果 壮、汉族证型分布规律:壮族各证型分布规律,382例壮族人群高血压病各证型分布规律,虚证:阴虚阳亢证＞肾气亏虚证;实证中痰瘀互结证＞肝火亢盛证＞气滞血瘀证.327例汉族人群高血压病证各证型分布规律,虚证:阴虚阳亢证＞肾气亏虚证;实证中痰瘀互结证＞肝火亢盛证＞气滞血瘀证,壮族与汉族高血压病中医证型分布差异具有统计学意义(P =0.000).高血压病中医证型在壮、汉族同性别分布差异性有统计学意义(P＜0.05);壮族男性与女性高血压病各证型分布差异无统计学意义(P =0.626),汉族男性与女性高血压病各证型分布差异具有统计学意义(P=0.000).壮、汉族高血压病中医证型在不同年龄段分布差异具有统计学意义(P＜0.05).心血管危险因素:汉族人群BMI、甘油三酯、肌酐更高(均P＜0.05);壮族人群总胆固醇、低密度脂蛋白、高密度脂蛋白更高(均P＜0.05);汉族人群高血压病家族史高于壮族(P＜0.05);壮族人群与汉族人群空腹血糖、C反应蛋白、吸烟史及饮酒史无显著性差异(均P＞0.05).结论 痰瘀互结及肝火亢盛为壮、汉两族的高血压病主要中医证型,壮族与汉族高血压病中医证型分布具有差异性.壮、汉民族高血压病中医证型在同性别分布具有差异性,壮族男女性别高血压病中医分布证型无差异性,汉族男女性别之间具有差异性.壮、汉民族高血压病中医证型在不同年龄段分布具有差异性.壮族与汉族人群高血压病家族史、BMI、甘油三酯、肌酐、总胆固醇、低密度脂蛋白、高密度脂蛋白等具有差异性;壮族人群与汉族人群空腹血糖、C反应蛋白、吸烟史及饮酒史无显著性差异.研究显示壮族地区壮、汉两族高血压病中医证型分布及心血管危险因素有一定的规律和特殊性.

This paper was aimed to study the effectiveness and safety of TanshinoneⅡA Sulfonate Sodium Injection in the treatment of unstable angina pectoris (UAP). Keywords such as coronary atherosclerotic heart disease, coronary heart disease, unstable angina, chest impediment, cardialgia, TanshinoneⅡA Sulfonate Sodium Injection, tanshinone injection, tanshinoneⅡA sulfonate, unstable, angina, randomized controlled trial (RCT), and clinical trials were searched in CNKI, VIP, Wanfang and Pubmed from the construction of database until October 31st, 2014. The inclusion criteria were RCT with clinical data integrity, similar literature research methods, and good balance between groups. The Jadad score method was used to carry out quality assessment. Meta-analysis was conducted by RevMan5.2 software. Count data was processed by odds ratio (OR). Measurement data was processed with the weighted mean differences (WMD). The 95% confidence interval (CI) was also calculated. The heterogeneity test result of included literatures was P > 0.05. The fixed effects model was used in the meta-analysis. On the other hand, random effect model was used. For the analysis results of more than 10 papers, the funnel plot was used in the analysis of publication bias. The results showed that a total of 34 studies were included. The results of meta-analysis suggested that the total efficiency of conventional treatment plus TanshinoneⅡA Sulfonate Sodium Injection for UAP was [OR = 3.83, 95%CI (3.11, 4.71),P < 0.000 01]. The electrocardiogram improvement rate was [OR = 3.34, 95%CI (2.61,4.28),P < 0.000 01]; plasma viscosity improvement was [WMD = -0.20, 95%CI (-0.38, -0.03),P = 0.03]; high shear viscosity of whole blood improvement was [WMD = -0.67, 95%CI (-0.85, -0.50),P < 0.000 01]; C-reactive protein improvement was [WMD = -2.66, 95%CI (-3.31, -2.00),P < 0.000 01]. It was concluded that the conventional treatment plus TanshinoneⅡA Sulfonate Sodium Injection for UAP had certain clinical effect with no obvious adverse reaction. However, due to the poor quality of the existing research literatures, the results should be further verified by large amount of high quality RCTs.

原发性高血压是诱发中风、心肌梗死、充血性心力衰竭和动脉瘤破裂的危险因素,也是严重危害健康的心血管疾病之一.2013年世界健康日把高血压作为全球关注的重大公共卫生问题.高血压在全球范围内呈不断增长的趋势,在全球每年940万的死亡人数中,由高血压引起的占13％,同时高血压的致残率为7％[1].而高血压与内皮功能障碍关系密切,内皮损伤与恢复关系对心血管事件的发病起到重要作用[2].高血压导致内皮损伤,引起内皮功能障碍、血管壁重塑,这种血管重塑过程使外周阻力升高,对血管内皮的损伤进一步加重,构成恶性循环.内皮祖细胞是一种多能于细胞,是内皮细胞的前体,内皮祖细胞数量的下降及其功能(主要是参与出生后缺血组织的血管发生和血管损伤后的修复)受损是高血压发病的一个重要因素,这一因素已被相关研究证实[3].因此,本文对近年来内皮祖细胞与高血压关系的研究进展作一综述.