[Objective] To study the effect and mechanism of Epicatechin (EC) and its derivate Epicatechin gallate (ECG) in cynomorium songaricum on the proliferation of prostrate stromal cell line WPMY-1. [Methods] WPMY-1 cells were treated with different doses of ECG and EC in vitro. MTT assay was performed to detect the effects of ECG and EC on WPMY-1 cell proliferation. PCR and western blot were performed to detect the mRNA and protein eXpressions of proliferating cell nuclear antigen proliferating cell nuclear antigen(PCNA) separately in WPMY-1 cells treated with ECG or EC. The phosphorylation levels of MAPK signaling pathway ERK44/42, P38, and SAPK/JNK were then detected by western blot in WPMY-1 cells after treatment with ECG or EC. The effects of different MAPK signalings on ECG and EC-suppressed cell proliferation were investigated in WPMY-1 cells after treatment with or without ERK44/42 agonist C6-Ce-ramide (C6) or dual agonist Anisomycin (ANI) of P38, SAPK/JNK. [Results] Both ECG and EC (1 nmol/L to 10μmol/L) inhibited the proliferation of WPMY-1 cells significantly (P<0.01) and PCNA eXpressions in mRNA and protein levels in WPMY-1 cell(P<0.05). the constitutive phosphorylation levels of ERK44/42 and SAPK/JNK were significantly suppressed by ECG and EC, which of P38 was not al-tered. After the adding of C6 blocked ECG and EC-induced suppression of WPMY-1 cell proliferation(P<0.05 or P<0.01). The change of cell proliferation was not significant after the adding of ANI. [Conclusion] ECG and EC inhibit prostatic stromal cell proliferation through ERK44/42 pathway, which provides evidence for their mechanism on benign prostatic hyperplasia prevention.

目的：观察中药内服、外敷联合刺络拔罐治疗中重度寻常型痤疮的临床疗效。方法：患者随机分为治疗组和对照组，治疗组予中药内服、刺络拔罐和面部中药湿敷疗法后倒模，对照组仅予中药内服，治疗3个月后观察并比较疗效及皮损情况。结果：治疗后2组患者丘疹、脓疱、结节囊肿、粉刺积分及皮损总积分均较治疗前明显降低，治疗组上述各项评分均明显低于对照组；治疗组总有效率明显高于对照组。结论：采用中医综合疗法治疗中重度寻常型痤疮效果优于单纯服用中药，值得临床推广。

With the development of modern trauma technology,wound dressing not only covers the wound,but more importantly it also has a function to promote wound healing. From the 1960 s to now,many scholars,through a lot of wounds experimental research and clinical practice,have proved that the wound dressing has the function to promote wound healing. How to better implement the wet environment healing theory currently,at the same time,how to make good use of the advantages of traditional Chinese medicine,will be the future direction of the research and development of new wound dressings.

[Objective] To establish an acne model of rabbit ear and then observe the therapeutic effect of compound traditional Chinese medicine acne gel. [Methods] Pix carbonis dilution(2% concentration of pix carbonis solution with 95% concentration of alcohol) was applied to inside surface of rabbit ears for successive 14 days to prepare the experimental acne model on rabbit ear. The models were confirmed by both naked eye and pathology examination. They were then divided into group A, B, and C randomly, applying 5% acne gel, 10% acne gel and tretinoin to the lesions respectively. After treatment for two weeks, the acne was classified according to a standard and the therapeutic effects was compared between these three groups. [Results] In contrast, these three treatments showed remarkable therapeutic effects compared with before. But the differences among three groups were not significant. [Conclusion] Compound Chinese medicine acne gel has a specific therapeutic effect on experimental rabbit ear acne models.