In order to investigate Kcnq1 gene function in golden hamsters,Kcnq1 gene was cloned and identified,and its expression differences was analysed in this study.Total RNA was extracted from golden hamster heart and primer TK1 was designed according to the rats Kcnq1 conservative sequence.Kcnq1 cDNA fragment was amplified from the heart using RT-PCR method.The purified cDNA was specifically linked with pMD18-T vector using the heart cDNA fragment as a template,which was then transformed into competent E.coli DH5α.After that,the recombinant was screened and identified and DNA sequencing.Total RNA was extracted from golden hamster heart,liver,spleen,lung and kidney,and Kcnq1 cDNA fragment was amplified using RT-PCR,expression differences in a variety of organs were detected by Real time PCR.The golden hamster Kcnq1 gene was cloned and total length was 477 bp,which indicated that it encoded 159 amino acids.The nucleotide and amino acid sequences had high homology when compared with rat etc..Real time PCR results showed that Kcnq1 gene was highest highly expressed in the heart,lung and kidney,less expressed in the spleen,almost no expressed in the liver.The expression in the heart was the highest.This study laid the foundation for further investigation for Kcnq1 gene function in golden hamsters.

The expression of Nramp 1(Slc11a 1) in late phagolysosomes confers resistance to infection with several intracellular pathogens.The antimicrobial actions of Nramp 1 are attributable,in part,to modulation of macrophage immune function and cellular iron metabolism.In order to investigate the function of goat natural resistance associated macrophage protein and to find a effectively way on natural resistance to infection in goat.Nramp 1 gene was cloned from goat spleen by RT-PCR and was inserted into pcDNA3.1(+),which named pcDNA3.1(+)-Nramp 1.Getting 60 days small tail han sheep fetal to establish fetal fibroblast cell lines while the linearized vector was transfected into sheep fetal fibroblast cells to gain stably transfected cell lines.However,the identification at the genome and transcription level shows that the exogenous gene successfully integrated into the genome and the gene expression is detected at transcription level.Two transgenic cell lines were carried out and laid a foundation of Nramp 1 protein function researching and antibacterial experiments.

V13KL is a kind of antimicrobial peptide,which is obtained by chemical synthesis. It is with characteristic of highly efficient, broad-spectrum and low hemolysis. First, V13KL encoding gene fragment was cloned into the pro- karyotic expression vector pET30b （＋）, and enterokinase restriction sites was designed at N-terminal. Then, pET30b（＋）-VK13L recombinant plasmid was transformed into E. coli expression strain BL21 （DE3）. The induction conditions were optimized, which make the peptide highly expressed. The identification by Tricine-SDS-PAGE and Western blotting indicated that the molecular weight of the protein was as expected. The results showed that V13KL gene was successfully cloned and expressed , and the protein expression could be detected in the induction system when 1 mmol/L IPTG was added up to 6 h.

This study was designed to investigate the single nucleotide polymorphism(SNP) of myostatin(MSTN) gene in red steppe and to analyze by PCR-SSCP,at the same time the correlation between the polymorphisms and several carcass traits.The results of cloning and sequencing indicated that polymorphism of intron 1 dued to base mutation of C→T in position 1 452 bp,and there were three genotypes(AA,AB and BB).The correlation analysis revealed that polymorphisms had a significant effect on net meat percentage,the BB and AB genotype had higher net meat percentage than the AA genotype in red steppe(P0.05),but there were no significant differences between BB genotype and AB genotype(P0.05).Other carcass traits of red steppe was not significant in different genotypes(P0.05).It could be preliminarily concluded that MSTN gene was probably a major gene or a QTL linked gene which associated with meat quality traits in red steppe.

This paper deals with the following Non-Newtonian polytropic filtration equation ut=div(|▽um|p-2▽um) possessing the extinction and positivity of the solution by comparison principle and fundamental solution for m0,p1 under initial value u0(x)∈L1(Ω) and zero boundary value conditions.

This paper deals with the following Non-Newtonian polytropic filtration equation ut=div(|▽um|p-2▽um) possessing the extinction and positivity of the solution by comparison principle and fundamental solution for m>0,p>1 under initial value u0(x)∈L1(Ω) and zero boundary value conditions.

In order to investigate the correlations between the blood protein polymorphic points and performance of Red Steppe,the polyacrylamide gel electrophoresis(PAGE)were employed to detect hemoglobin(Hb),albumin (Alb),postalbumin(Pa)-three blood protein polymorphic points of 13 Red Steppe and 18 limousin×Red Steppe.The results showed that Hb,Alb and Pa cotroled by 2 allelomorph respectively;the 4 bands of Hb had dissociation in 1 Red Steppe.The of variance analysis of the blood protein polymorphic points and performance suggested 3 protein points had positive or negative effects on some traits of Red Steppe and limousine.

[Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig×Sus scrofa,so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template,RT-PCR amplification was carried out for cloning cDNA of hybrid pig,which was then used for PCR reaction using specific primers;the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig×Sus scrofa. [Result] H-FABP expressed in all the twelve tissues including subcutaneous fat,abdominal fat,mammary gland,longissimus dorsi muscle,dorsal deltoid muscle,heart,liver,spleen,lung,kidney,ileum and duodenum. [Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.

[Objective] The aim of this study was to investigate the correlation between blood protein polymorphism of red steppe and its performance.[Method] Two blood protein polymorphic loci were detected in transferring(Tf) and posttremsferr(Ptf) from thirteen red steppes and eighteen hybrid of limousin and red steppe by polyacrylamide gel electrophoresis.[Result] Tf and Ptf were controlled by three and two alleles respectively.[Conclusion] The variance analysis of blood protein polymorphic loci and its performance indicates that two protein loci have a positive or negative correlation with some traits of red steppe and the improved limousin cattle population.