OBJECTIVE: To explore the characteristics of primary liver cancer in terms of Traditional Chinese Medicine(TCM) by analyzing the variations of the patterns along with the clinical stages.METHODS: The patients who were hospitalized in the Changhai Hospital of Traditional Chinese Medicine dated from March 1999 to December 2008 were included in this retrospective study. The patients were grouped according to their cancer stages, and their patterns were judged and quantified according to the 'Standard diagnosis and quantitative criteria of the common patterns in primary liv-er cancer' formulated by the Changhai Hospital of Traditional Chinese Medicine. Statistics methods included ANOVA and nonparametric test, among others.RESULTS: The data of the 398 newly diagnosed patients showed that Qi Stagnation, Blood Stasis, and Dampness patterns were more frequent than the other basic patterns with relatively high scores; patterns of Liver Qi Stagnation, Liver Blood Stasis, and Dampness Heat were more than the other complex patterns and scored relatively high. Scores of Dampness and Liver Qi Stagnation patterns varied among the groups at different stages and the differences were statistically significant(PDampness= 0.002,PLiver Qi Stagnation= 0.020). The highest scores of Dampness pattern and Liver Qi Stagnation pattern corresponded with Stage Ⅲb, and Stage equency wⅢ a, respectively. Dampness pattern fras higher(P =0.001) in the Stage Ⅲb group than in other groups.CONCLUSION: Pattern characteristics in patients with primary liver cancer of different clinical stages might manifest in the variations of the Dampness pattern along the process of the disease and the major pathogenic factor of primary liver cancer might be Dampness.

目的 合成具有抗肿瘤活性的二十六肽蜂毒素.方法 采用Fmoc固相逐步化学合成,确立了以Wang树脂为固相载体,HOBt/ DCC为缩合剂的合成工艺.结果 通过本法能够顺利合成得到二十六肽蜂毒素,收率达32％.结论 该合成方法具有可行性强、操作简便、总收率高等特点,适用于长链多肽的合成.

目的 观察原发性肝癌患者手术前后舌象特征变化,探讨肝癌围手术期患者证候特征.方法 收集103例拟行手术的原发性肝癌患者,于术前1天及术后1、3、5天上午6:30-8:00观察患者舌象,填写中医舌象调查表,用数码照相机留取舌体图片,采用中医舌诊综合信息处理系统分析舌质及舌苔颜色平均RGB各分量值.结果 定性及定量结果均提示,术前患者以青紫舌为主,术后1天以红舌为主,术后3、5天青紫舌占居首位(P＜0.05);术后舌质颜色R分量值升高,以术后第1天最明显,术后3、5天舌质颜色R分量值逐渐下降(P＜0.05).术后3、5天较术前1天舌苔R、G、B各分量值逐渐下降(P＜0.05).术后舌形胖大、齿痕、点刺舌有增多趋势(P＜0.05).手术前后皆以厚腻苔为主,且术后较术前比例逐渐上升(P＜0.05).术后1、3、5天较术前1天舌下络脉宽度明显降低(P＜0.05).结论 血瘀、水湿、气虚证舌象贯穿原发性肝癌患者围手术期,术后5天内阴虚内热舌象比例有所增加.

Objective: By observing the change in pulse manifestation characteristics before and after operation in patients with primary liver cancer,it is to provide data support that traditional Chinese medicine will be applied to perioperative patients with hepatocellular carcinoma. Methods: The pulse graph parameters with left and right hands Guan's pulse were collected by using SMART MX-1 I type pulse instrument 6∶ 30- 8∶ 00 in the morning of 1 days before operation,1,3,5 days after operation. The collected data was analyzed with SAS software. Results: The third days after operation patients' left hand pulse rate were faster than the first day before operation( P 0. 05). After operation patients' right hand pulse rate were faster than the first day before operation( P 0. 05),and the most obvious change was in the third day after operation. Conclusion: Xian( taut) and slippery pulse is throughout the perioperative patients with primary liver cancer. Pulse rate are accelerated after surgery,changing to rapid pulse.

目的:探讨慢性乙肝病毒携带者不同中医证型与HBV基因型及HBV DNA载量的关系。方法:对95例慢性乙肝病毒携带者进行临床流行病学调查,组织专家组对其进行中医辨证,同时检测HBV基因型及HBV DNA载量,进行统计学分析。结果:慢性乙肝病毒携带者的中医证型以肝胃不和、肝气郁结、肝阴虚、肾阴虚证为主。4个主要证型间的HBV DNA载量比较,肝阴虚证>肝气郁结证>肝胃不和证>肾阴虚证。不同HBV基因型中HBV DNA载量比较,C型>B型。结论:不同中医证型与HBV基因型及HBV DNA载量间有一定的规律性,但无明显的相关性。

[Objective] To determine the impact of transcatheter arterial chemoembolization(TACE) in primary hepatocellular carcinoma(PHC) by comparing the syndromes.[Method] From March 2005 to December 2008,274 PHC patients who were hospitalized in the Traditional Chinese Medicine Department of Changhai Hospital and who had only undergone TACE were included.Syndromes of the patients were judged and collected before and after the treatment.With portal vein tumor thrombus(PVTT) or not,the patients were grouped and their data were analyzed again.[Result] Data of the 274 patients demonstrated that,among all the simple syndromes,level of Qi Deficiency increased after TACE,and momentum showed level of Blood Stasis was decreasing while that of Dampness Syndrome was rising;as to complex syndromes,level of Liver Qi Stagnation increased,level of Liver Blood Stasis had the rising trend while that of Dampness Heat Syndromes had the decreasing trend.The aggravating of Dampness Syndrome and Spleen and Kidney Yang Deficiency was severer in patients with PVTT than in those without.Profiles of the patients underwent TACE for the first time revealed that scores of Qi Stagnation,Excessive Heat,Liver Qi Stagnation Syndrome and Spleen Qi Deficiency Syndrome decreased after the treatment.[Conclusion] Impact of TACE on syndrome gave the evidence of local improvement and general impairment in patients with PHC.

[目的]初步探讨经动脉化疗栓塞(TACE)治疗对肝癌患者证候的影响.[方法]收集2005年3月至2008年12月长海医院中医科收治的274例次仅行TACE治疗的肝癌患者,采用配对检验等统计学方法对患者治疗前后证候的变化进行统计分析,并将有无癌栓作为分组因素,对患者TACE治疗前后证候的变化进行对比分析.[结果]274例次肝癌患者行肝脏TACE术后基本证候中气虚证程度加重(P＜0.05),血瘀证有减轻趋势,水湿证有加重趋势；复合证候中肝气郁结证程度减轻(P＜0.05),肝血瘀阻证有减轻趋势,湿热内蕴证有加重趋势.有癌栓者治疗后水湿证及脾肾阳虚证加重程度较无癌栓者大(P＜0 05).[结论]TACE治疗对证候的影响体现了肿瘤病情的好转及机能的损耗.

OBJECTIVE: To investigate the application of Traditional Chinese Medicine Injections (TCMIs) for treatment of primary liver cancer (PLC).METHODS: A literature review was conducted using PubMed/Medline, Cochrane Library Controlled Clinical Trials Database, China National Knowledge Infrastructure (CNKI), China Scientific Journal Database (CSJD) and China Biology Medicine (CBM). Online websites including journal websites and databases of ongoing trials, as well as some Traditional Chinese Medicine journals that are not indexed in the electronic databases were also searched.RESULTS: The literature review showed that TCMIs as adjunctive medication for the treatment of PLC could regulate patient immunity, reduce bone marrow suppression, relieve clinical symptoms, and improve quality of life, as well as control disease progression and prolong survival time.CONCLUSION: Within the limitations of this review, we conclude that application of TCMIs as adjunctive medication may provide benefits for patients with PLC. Further large, high-quality trials are warranted. (C) 2012 JTCM. All rights reserved.

OBJECTIVE To explore the protective effects of Huqi extractum, a compound Chinese herbal medicine, on salivary glands against radiation in Wistar rats. METHODS One hundred Wistar rats were randomly divided into sham-exposure group, untreated group, and low-, medium- and high-dose Huqi groups. Local irradiation of 60Co gamma-rays with a single dose of 15 Gy was applied to the salivary glands of the Wistar rats except the sham-exposure group. After 3- and 40-day treatment, saliva was collected. Colorimetric method, iodine-amylase colorimetric method and enzyme-linked immunosorbent assay were used to detect concentrations of sodium (Na+), potassium (K+) and secretory immunoglobulin A (sIgA) and activity of salivary amylase. Pathological changes of salivary gland tissues were observed by hematoxylin-eosin straining. RESULTS After 3-day administration, radiation-induced salivary gland injuries were obvious and prevalent in irradiated rats. Comparing with the sham-exposure group, saliva concentration of sIgA and body weight were reduced in other irradiated groups, except those in the high-dose Huqi group, while salivary amylase level was increased. At 3-day phase, pathologic changes of the salivary glands were featured as swelling acinus plasm and vacuolation. At 40-day phase, atrophy of gland cells was dominant. After 40-day administration, there were no significant differences between the high-dose Huqi group and sham-exposure group in sIgA and amylase levels and body weight, and according to the histological examination, no significant difference was revealed under the optical microscope. CONCLUSION Chinese herbal medicine is helpful for the recovery of the salivary glands from the radiation injury, morphologically and functionally in rats.

Objective To study the TCM syndrome distribution of h epatitis B virus infection people in Qidong region of Jiangsu province. Methods HBsAg screening was carried out in the local community residents enrolled. A study queue of hepatitis B virus infection people with HBsAg positive was set up, including hep atitis B virus carrier, chronic hepatitis B, cirrhosis, and primary liver cancer . According to the syndrome standard, the basic syndromes and composite syndrome s of hepatitis B were determined, and then basic syndromes were quantitatively s cored. Results The first four basic syndromes were qi s tagnation (28.8%), bloo d stasis (26.2%), qi deficiency (14.0%), and excess heat (10.7%). The first fou r composite syndromes were liver qi stagnation (29.5%), liver qi stagnation with spleen deficiency (19.0%), liver qi stagnation with blood stasis (16.9%), and yi n deficiency of liver and kidney (13.7%). There was significant difference in the score of q i stagnation syndrome between the cirrhosis group and the other 3 groups (P 0.05). There was significant difference in the score of excess heat syndrome between the hepatit is B virus carrier group and chronic hepatitis B as well as cirrhosis groups (P0.05). And there was significant difference in the scores of water damp and qi deficiency syndromes between the hepatitis B virus carrier group and the other 3 groups (P0.05 ). Conclusion The hepatitis B virus infection people in Qidong region of Jiangsu province is characterized by combination of deficiency with excess, and the develo pment of pathological condition is manifested by the degree of qi stagnation, qi deficiency, excess heat, and water damp syndromes.