目的 通过调查海口市大学生网购食品知识、态度及行为现状,找出目前存在的问题并分析其原因,为网购食品安全管理提供理论参考.方法 通过自行设计的问卷,采用随机抽样方法,对海口市五所大学的1000名学生进行调查.采用卡方检验和非参数检验方法对结果进行分析.结果 不同学校、专业、性别、学历、年级及是否接受过专业的食品安全教育对网购食品安全知识评分差异有统计学意义;不同学校、学历及是否接受过专业的食品安全教育对网购食品安全行为评分差异有统计学意义.在抽样调查的123名大学生中不愿意网购的原因是觉得网上食品不可靠占比65.9%、需等待与物流不及时占比41.5%.结论 海口市大学生食品安全知识的知晓率较低,食品安全消费行为还存在很多问题,后期应该加强学生网购食品安全防范意识的培养和教育.
该文以海口市为例,利用协整分析和Granger因果检验对海口市2007年-2016年医疗卫生机构数以及国民经济核算2个时间序列进行研究.Granger因果检验发现国民经济核算是医疗卫生机构数的单项格兰杰原因.协整检验显示2007年-2016年医疗卫生机构数与国民经济核算之间存在着一种长期均衡稳定的关系.
目的:了解海南省本科大学生创新创业训练计划的参与度情况,为调动大学生参加大学生创新创业训练项目给出既合理又科学的建议.方法:采用随机抽样方法对海南高校大学生创新创业训练项目的参与度情况进行问卷调查.结果:被调查的大学生参与度为14.04%,在学习成绩角度排名前10%参与度最高(17.1%),具有统计学意义.结论:海南省本科大学生对"大创"项目的参与度较低,应针对其影响因素,采取措施调动大学生参与大学生创新创业训练计划的积极性.
Objective: Both antibiotic resistant bacteria (ARBs) and antibiotic resistant genes (ARGs) are considered as one of the most dangerous environmental pollutants in the 21st century by the WHO. This study aims to understand the ARGs of Klebsiella pneumoniae (K. pneumoniae) with multiple drug resistance bacterium from the marine water collected from a beach at Sanya Bay, and to explore the antibiotic resistance mechanism of the K. pneumoniae, providing a basis for exploring the transfer of drug resistance genes of beach, and preventing and controlling the health risk of entertainment population. Methods: The sample of marine water were collected and screened by Mcconkey plate. The drug sensitive test was detected by Merieux VITEK2, The DNA was extracted and one strain of 16srDNA was sequenced and identified as K. pneumoniae. Whole-genome resequencing was performed using Illumina HiseqXten platform, and the obtained sequences were compared with NCBI blasting. The reference bacterium were multi-resistant K. pneumoniae HS11286. Plasmids were extracted and the resistant genes were identified. Results: The ARGs encoding protein was 117/4801 (identity > 40%) and the carrying rate was 2.436 9%. The identity of following ARGs of OKPB, sul1, rpoB, ef-tu, phoP, sul2, AAC(6’)-ib-cr, QnrB, floR, aadA16 were more than 99%. The strain showed resistance to ampicillin, ticacillin/clavulanic acid and chloramphenicol, and was intermediate to ampicillin/sulbactam, compound sulfamethoxazole, ciprofloxacin, minocycline. Preliminary positioning showed that Qnrs, sul1, tetA, cat, QacE were carried on plasmid. Conclusion: The multiple drug resistant bacteria strain has a variety of different resistant phenotypes, some ARGs can be easily spread by plasmid. It probably will bring exposure risk to people for entertainment. Sensibility of some antibiotics were on the brink of resistance, It is necessary to tracking corresponding antibiotics pollution and strengthening monitoring of ARBs and mobile resistant elements of bacteria.
本文采用随机抽样方法对海南高校大学生的"大创"知晓率进行问卷调查.结果被调查的大学生知晓率达70.53%,其中女生知晓率高于男生(74.1%);大五学生知晓率高于其他年级(91.7%);在专业方面政法科最高(85.7%);在成绩情况角度排名10%知晓率最高(76.05%);均具有统计学意义.结论海南省本科大学生对"大创"项目的知晓率并不高,"大创"是教育部高度重视的科研项目,学校应该继续增加宣传力度.