实施课程思政是高校落实立德树人根本任务的重要举措.阐述高职畜牧兽医基础应用化学课程可以融入的课程思政元素,提出了通过组建团队,提升授课教师开展课程思政建设能力;在课程标准中确立课程思政的教学目标;不断优化蕴含思政教育的教学设计方案等方法,将育人贯穿课堂教学课前、课中、课后全过程,使学生将思政内化于心、外化于行,在教学过程中实现立德树人.

为研究利用不同佐剂制备的猪繁殖与呼吸综合征病毒NADC30(NADC30-PRRSV)SY株灭活疫苗的免疫效果,本研究对由ISA201VG和ISA70VG佐剂分别制备的2种NADC30-PRRSV SY株疫苗的免疫效果进行比较研究.本研究选用35日龄PRRSV抗体和抗原均为阴性的健康仔猪15头,随机分为3组:ISA201VG免疫组、ISA70VG免疫组和未免疫攻毒组.免疫组首免107.0 TCID50/头,间隔21 d后以相同剂量进行二次免疫,采用ELISA法检测PRRSV血清抗体,结果显示,免疫后第35 d,ISA70VG免疫组5头试验猪PRRSV抗体阳转率达100％,ISA201VG免疫组5头试验猪PRRSV抗体阳转率达40％.二免后14 d采用NADC30-PRRSV SY株攻击试验猪,攻毒剂量为1×105.0 TCID50/mL,检测各组猪体温度化,结果显示,ISA70VG免疫组试验猪从攻毒后第8 d起体温超过40℃,持续6 d;ISA201VG免疫组5头试验猪只有1头体温超过40℃;未免疫攻毒对照组5头猪攻毒后第11 d～13 d体温升至40℃持续3 d.各组猪增重检测显示,ISA70VG佐剂免疫组与未免疫感染对照组相比较,体重呈现负增长且体重减少均在5％以上,而ISA201VG与未免疫感染组相比较,体重呈现正增长且增长均在7％以上.对PRRSV感染后两个实验组肺组织病理观察均可见肺脏呈局限性间质性肺炎和淤血,并伴有血栓、部分肺泡隔增宽,支气管周围有淋巴细胞浸润伴有间质性肺炎,但ISA70VG组比ISA201VG组组织病变更加严重.本实验表明ISA201VG佐剂效果更好,同时首次证实类NADC30-PRRSV(SY株)也能导致严重抗体依赖增强效应,不益作为疫苗株.

鸭瘟病毒UL36蛋白是该病毒皮层结构的主要成分,也是主要的抗原蛋白.本文利用生物信息学方法就基因序列与蛋白组分分析、信号肽预测、跨膜区预测与亚细胞定位等方面对该基因及编码蛋白序列进行分子特性分析,以便为后期实验研究提供参考依据.

为了能培养出适应新时代需求的人才,在高职教育过程中,以学校、企业和行业共同合作构建平台,实行现代学徒制的人才培养模式,取得了一定的成效,进一步深化了高校人才培养模式.分析了现代学徒制的内涵及实施的必要性,并对内江职业技术学院畜牧兽医专业现代学徒制试点的基本思路、做法及面临的困难进行了探讨,以期对高职院校现代学徒制人才培养有所帮助.

高职院校课程思政教学团队的建设是推动高职院校思想政治教育创新,将社会主义核心价值观融入人才培养全过程,推动人才培养素质培优的重要力量.以内江职业技术学院畜牧兽医专业课程思政教学团队的建设为例,通过发挥课程思政教学团队优势,汇聚形成强劲整体合力,打造有专业特色的课程思政体系,提升专业教师的立德树人能力,更好地培养服务"三农"的高素质技术技能人才.

文章旨在研究饲粮中添加黄芩提取物对肉鸡生长性能、营养物质消化率和肉质的影响.试验选取平均体重接近的1日龄AA肉鸡100只,称重后随机分为4组,试验1、2、3组在对照组基础饲粮基础上分别添加0.02％、0.04％、0.06％的黄芩提取物,处理42?d.试验结果:与对照组相比,试验2、3组肉鸡21日龄体重分别显著提高10.5％、9.3％(P<0.05),试验1、2、3组肉鸡42日龄体重分别显著提高8.7％、11.9％、10.4％(P<0.05).1～21日龄,试验2、3组肉鸡ADG分别显著提高11.2％、10.0％(P<0.05);22～42日龄,试验1、2、3组肉鸡ADG分别显著提高9.5％、12.5％、10.9％(P<0.05),试验2、3组FCR分别显著降低12.7％、8.2％(P<0.05);1～42日龄,试验1、2、3组肉鸡ADG分别显著提高8.8％、12.2％、10.7％(P<0.05),试验2、3组FCR分别显著降低11.0％、7.8％(P<0.05).试验1、2、3组肉鸡对饲粮中氮的消化率较对照组分别显著提高8.2％、9.8％、7.5％(P<0.05),试验2、3组肉鸡对GE的消化率分别显著提高8.5％、7.3％(P<0.05),试验2组肉鸡对饲粮中DM的消化率提高8.0％(P<0.05).试验1、2、3组肌肉第5天的滴水损失较对照组分别显著降低14.9％、15.7％、15.2％(P<0.05).表明,黄芩提取物可以促进肉鸡生长,改善养分消化率和肉质,添加量为0.06％时效果最好.

文章旨在评价植物乳杆菌对感染产肠毒素大肠杆菌断奶仔猪生长性能和细胞因子反应的影响.试验选择216头21 d的断奶仔猪,随机分为6组,其中T0为对照组(未感染细菌),T1感染6×109 CFU产肠毒性大肠杆菌,T2感染组+抗生素,T3～T5组分别为感染组+1×108、1×109和1×1010 CFU/kg植物乳杆菌,试验共进行28 d.结果:攻毒后,T1组采食量最低,T2和T5的采食量最高(P<0.05).从第1～2周,T2、T4和T5组日增重显著高于T0、T1和T3组(P<0.05).在3～4 w期间,T2组较T0组显著降低了日增重(P<0.05),而T2和T5组日增重显著升高(P<0.05).T2和T5组在第2周的饲料效率最大(P<0.05).除T4外,所有攻毒猪的血清结合蛋白浓度均高于T0组,其中T1组最高(P<0.05).攻毒后3 h,T2组IL-6浓度低于T3、T4、T5组(P<0.05),TNF-α 浓度从攻毒后0 h到48 h显著升高(P<0.05).综上所述,断奶仔猪日粮中添加1010 CFU/kg植物乳杆菌可以作为潜在的抗生素替代物,改善断奶猪生长性能和健康,尤其是在急性产肠毒素大肠杆菌感染时.

文章旨在研究日粮添加不同蛋白原料(豆粕、葵花粕或羽扇豆)对生长育肥期肉兔生长性能、养分消化率及屠宰性能的影响.试验选择37 d断奶的肉兔120只,随机分为3组,每组4个重复,每个重复10只兔.试验设计3种日粮,日粮中蛋白原料分别是豆粕(10％)、葵花粕(17％)和羽扇豆(15％),试验共进行6周.结果:葵花粕组较豆粕和羽扇豆组有降低肉兔总能、酸性洗涤纤维和淀粉表观消化率的趋势(P=0.06,P=0.08,P=0.09),同时中性洗涤纤维显著低于其他两组(P<0.05).豆粕组较葵花粕和羽扇豆组有提高其他挥发性脂肪酸含量的趋势(P=0.08),同时豆粕组有提高盲肠及其内容物相对重量的趋势(P=0.09,P=0.06).与豆粕组和葵花粕组相比,羽扇豆组显著提高了盲肠乳酸含量(P<0.05).葵花粕组较其他组显著提高了肉兔各阶段平均日采食量(P<0.05).葵花粕组较羽扇豆组显著提高了59～79 d肉兔的死亡率(P<0.05).羽扇豆组较豆粕组和葵花粕组显著提高了肉兔的屠宰率(P<0.05).结论:羽扇豆是生长育肥肉兔饲料中较好的蛋白原料,可以完全替代饲料中的豆粕,对肉兔生长性能、屠宰性能以及养分消化、盲肠相关指标无负面影响.

本研究开展两个试验评估小麦DDGS日粮中添加酶对生长猪营养利用和生长性能的影响.试验一选择30 kg左右的生长猪6 0头,随机分为5组(每组12头),分别饲喂5种日粮,对照组日粮(玉米-大麦型)、基础日粮添加10％小麦DDGS(阳性对照组)、基础日粮添加10％小麦DDGS(阴性对照,不添加无机磷),以及分别在阴性对照组中添加500 FTU/kg植酸酶和500 FTU/kg植酸酶+0.5 g/kg多糖酶,试验为4周,目的是评估日粮处理对猪生长性能的影响.试验二选择22 kg左右的阉割公猪30头,随机分为5组,饲喂试验一的日粮,目的是测定营养物质的消化率和氮磷平衡.结果 显示:喂食小麦DDGS的阳性对照组和对照组之间的日增重和饲料报酬无显著差异(P＞0.05).小麦DDGS阳性对照组日增重和料重比均显著高于小麦DDGS阴性对照组(P＜0.05).小麦DDGS阴性日粮添加植酸酶改善了日增重、料重比和磷消化率(P＜0.05),较小麦DDGS阴性对照组提高了6.6％、8.7％、86.0％和85.5％.小麦DDGS日粮添加植酸酶和多糖酶对猪的生长性能无显著影响(P＞0.05),但显著降低磷的沉积率(P＜0.05).综上所述,在生长猪日粮中添加10％的小麦DDGS不会影响生长猪的生长性能,同时在含有小麦DDGS饲料中添加植酸酶可以降低无机磷的添加量.

繁殖性能是衡量一个规模化养猪场综合生产水平的一项重要指标,抓好母猪繁殖性能最理想时段的饲养管理,充分发挥生产潜力出来,保持稳定的生产性能.本研究重点对母猪生产性能中的畸形率、胎均弱仔、死胎、木乃伊胎、断奶活仔、成活率等方面进行分析,充分了解该猪场母猪的的生产性能.

硒是家禽和猪的基本营养物质,对许多生理过程都有重要作用.硒通过与蛋白质的结合参与氧化应激、氧化还原及先天和适应性免疫反应等过程.本文综述硒调节对传染病的抵抗机制及其如何影响家禽或猪在传染病感染期间维持生产性能的能力.

对发酵床养殖模式下王锦蛇肠炎的病因、临床症状、剖解变化、诊断方法进行了介绍,提出了王锦蛇肠炎的综合防治措施,为该病的防治提供临床参考.

2017年3月成都华西宠物医院收治了一例就诊病犬,临床表现为左后肢疼痛、不能负重,跛行,经临床诊断、实验室检查、DR检查诊断为左后肢股骨远端干骺处骨折.对病犬采取髓内针加"8"字拉力钢丝内固定术进行手术治疗后,取得了较好疗效.

我国畜禽日粮中广泛使用的硒源是亚硒酸钠,欧美国家则多使用蛋氨酸硒作为家畜的补硒制剂,而相对于亚硒酸钠、蛋氨酸硒等补硒产品,纳米硒不仅具有更高的吸收效率,而且毒性低,引起了畜牧业的广泛关注.本文综述了纳米硒的理化性质、生物学功能以及在反刍动物生产中的应用,旨在为其在反刍动物饲料中的应用提供理论依据.

The associations between SNPs in the PLTP gene and production performance of pigs were reported in this study.The results showed alleles detected in the sequence were allele A and a with frequencies of 0.4172 and 0.5828,0.4155 and 0.5845,0.4309 and 0.5691 in F4,F5,F6 generation,respectively.There were 4 mutations of 3 for conversion and 1 for transversion,4 SNPs(1085,1192,1286 and 1305 bp,respectively) were found by the sampling sequence.Associations between the founded Hae Ⅲ RFLP in intron 4 and birth weight,body weight at 45 days,body weight at 4 months,body weight at 6 months,body height at 6 months,body length at 6 months,cannon circumference at 6 months and back-fat thickness at 6 months were tested by least square analysis.In F4 generation,no significant difference between HaeⅢ RFLP and all growth traits(P0.05).In F5 generation,the birth weight and back-fat thickness at 6 months with Aa genotype were higher than that with AA genotype(P0.05).In F6 generation,the body weight at 4 months(P0.01)and body height at 6 months(P0.05) with aa genotype were higher than that with AA genotype.The cannon circumference at 6 months(P0.01) and the birth weight(P0.05) with Aa genotype were higher than that with AA genotype.In addition,the cannon circumference were the highest significant than that with AA genotype male(P0.01).The birth weight and back-fat thickness at 6 months were higher than that with AA genotype female(P0.05).These results suggested that the Aa genotype of PLTP gene had some effects on the growth traits of porcine.These SNP might be considered as a potential genetic marker for selection.

The associations between SNPs in the cholesteryl ester transfer protein(CETP) gene and production performance of pigs were reported in this study,the polymorphism of CETP gene was detected by a AvaⅡ RFLP in 334 Dahe Black pigs across F4 to F6 generations.These results showed that b alleles was excellent gene with frequencies of 0.5821,0.6042 and 0.6885 in F4,F5 and F6 generations,respectively.Seven SNPs(19,69,132,175,203,224 and 234 bp,respectively) were found by the homozygous samples sequencing.Associations between the founded AvaⅡ RFLP and birth weight,body weight at 45 days,body weight at 4 months,body weight at 6 months,body height at 6 months,body length at 6 months and cannon circumference at 6 months and back-fat thickness at 6 months were tested by least square analysis.The results showed that in F4 generation,body length at 6 months with Bb and bb genotype was higher than that with BB genotype(P0.05);cannon circumference at 6 months with bb genotype was higher than that with BB genotype(P0.05),that with Bb genotype was not significant(P0.05).In F5 generation,the birth weight with Bb and bb genotype was higher than that with BB genotype(P0.05),no significant difference between them(P0.05).In F6 generation,the birth weightand body weight at 45 dayswith bb genotype was higher than that with BB genotype(P0.05),that with Bb genotype was not significant(P0.05).In addition,the birth weight showed the higher significant difference between bb genetype and female/male(P0.05).These results suggested that the CETP gene had some effects on the growth traits of porcine,and the selection of b allect was favored with regard to the growth traits of porcine,this SNP might be considered as a potential genetic marker for improving growth.

在分析"校企合作、工学结合"对学院、企业、学生的三赢局面的分析及其必要性的基础上,从政策优势和行业优势方面阐述了"校企合作、工学结合"的广泛前景。结合当地情况和学院合作经验论证了"校企合作、工学结合"可行性,并提出了在实施过程中出现的相关问题。

Phospholipid transfer protein(PLTP) plays an important role in plasma lipoprotein metabolism.According to the gene function and gene homology,PLTP may have functions in both host defense response to pathogens and preimplantation embryo development.PLTP may be a major gene or genes linked to the major gene to affect economic traits.The genetic marker HaeⅢ established in future could be used for improving the birth weight and growth traits in marker-assisted selection program.

基因疫苗(genetic vaccine)又称核酸疫苗(nucleic acid vaccine)、DNA疫苗(DNA vaccine),是将带有目的编码蛋白抗原基因的真核重组表达载体直接注射到动物机体中,使外源基因在活体细胞内表达,诱导机体产生抗体并引起特异性的免疫应答,达到顶防和治疗传染病的目的.它包括DNA疫苗和RNA疫苗、其中DNA疫苗由于不需任何化学载体,故又称裸DNA疫苗.自Wolff等发表了"直接将基因转移至活体小鼠肌肉"一文以来,基因疫苗免疫已成为国内外防治传染病的研究热点.被称为疫苗学研究的新纪元,是疫苗研究的第三次革命.

Single nucleotide polymorphism(SNP) is the third generation of DNA markers after restriction fragment length polymorphism(RFLP) and microsatellite(or SSR). It's usually referred to as biallelic polymorphism. This paper introduced the character, discovery, screening, databases, frequencies and their association with phenotypes, as well as the research of SNP in chicken genetic varitation.