Objective To establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) using monoclonal antibody of bisphenol A (BPA) for the detection of serum BPA. Methods Totally 176 pupils aged 8-10 years were selected from three primary schools in suburban area of Nanchang city of Jiangxi province and serum BPA of the pupils were determined with ic-ELISA established. Results The detection limit of the established ic-ELISA method was 0.43 ng/mL. The recovery rates of BPA in serum spiked at 4 different levels were between 70.1％ and 87.8％ with the variation coefficients from 4.79％ to 9.41％. BPA was detected in 95 of the 176 serum samples and the detection rate was 54％. The serum BPA content ranged from non-detectable to 26.48 ng/mL for all the samples. The detection rate of serum BPA was higher among the boy pupils than among the girl pupils, but the detection rate was not significantly different among the pupils of various age groups (P =0.195). Conclusion The established ic-ELISA could meet the requirement of serum BPA detection among children. BPA was detectable in serum of children in Nanchang city of Jiangxi province and relatively higher serum BPA content was detected in some of the children, suggesting studies are needed to explore the contamination source of BPA.

为检测食品中苏丹红Ⅰ残留,建立间接竞争化学发光酶联免疫分析(chemiluminescent enzyme immunoassay,CLEIA)法.通过优化包被抗原中本抗原与载体物质的量比、包被抗原质量浓度、抗体稀释比例,建立竞争抑制曲线.线性范围为0.156～5 ng/mL,最低检测限为0.078 9 ng/mL,IC50为0.679 ng/mL.CLEIA回收率为75.08％～112.18％,变异系数为8.89％～15.61％;通过与酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)法进行比较,在相同抗原抗体质量浓度条件下,CLEIA法测定的IC50较ELISA方法降低30％,具有较高的灵敏度.

采用活泼酯法将双酚A的结构类似物双酚酸与载体蛋白偶联制备人工抗原,用制备的人工抗原免疫BALB/c小鼠,采用聚乙二醇法进行细胞融合制备双酚A单克隆抗体,成功获得一株分泌抗双酚A单克隆抗体的细胞株3H1,经鉴定抗体属于IgG1亚型,轻链为κ,并建立了间接竞争酶联免疫分析法.线性范围为1～50 ng/mL,最低检测限为0.43 ng/mL,半数抑制浓度为6.56 ng/mL.回收率为82.83％～101.94％,变异系数为2.94％～12.95％.该方法具有较高的灵敏度和特异性,具有良好的应用前景.

Fish process refuse, including fish head, fish bone, fish tail, fish viscera, swimming bladder, is about 50% of whole body weight, and leads to environmental problems as always discarded or buried. The active elements in the fish process refuse are summarized and further exploitation is prospected.

Fish bone is value for its nutrients, can be processed to be feature food. Calcium in fish bone can be processed to be calcium supplement. Fish bone also can be further processed for chondroitin sulfate, fishbone oil, protein.