Background:This study aimed to identify significant genes associated with cutaneous squamous cell carcinoma (CSCC) initiation and development.Methods:The overlapped differential expressed genes (DEGs) between normal and CSCC samples were firstly screened out, followed by KEGG analysis. The top 10 hub genes were then detected from the whole protein-protein interaction (PPI) network. Further, important biomarkers continuously associated with actinic keratosis (AK), CSCC, and CSCC invasion was successively filtrated. GSEA analysis was finally performed to reveal potential mechanisms associated with biomarkers.Results:A total of 179 DEGs were identified, which were enriched in pathways in cancer, PI3K-Akt signaling pathway, and human papillomavirus infection. The 10 hub genes were firstly identified from the PPI network, and they were all highly expressed in AK tissues compared with normal tissues. Next, we found that 6 genes were overexpressed in CSCC compared with AK tissues. Further, we identified that the expression of 2 genes (MYBL2 and TK1) was higher in CSCC invasion groups compared with samples without invasion. Through a series of filtrations, we confirmed that MYBL2 and TK1 were the most significant biomarkers associated with CSCC initiation and progression. The pan-cancer analysis further supported their prognostic value in human cancers. GSEA analysis found that they positively correlated with N glycan biosynthesis and p53 signaling pathways.Conclusion:MYBL2 and TK1 were proved to play a vital role in CSCC tumorigenesis and progression, which may act as promising biomarkers or therapeutic targets for accurate diagnosis and treatment of CSCC.
目的 探讨低浓度5-氟尿嘧啶(5-Fu)注射联合点阵激光在手术后增生性疤痕早期干预的临床疗效.方法 选取浙江萧山医院2017年5月—2019年5月的手术后增生性疤痕患者38例,将5-Fu注射到整条瘢痕下,随机选取一半侧瘢痕联合应用点阵激光为观察侧,另一半侧瘢痕为对照侧.比较患者两半侧增生性瘢痕美容评估与评级(SCAR)量表评分、瘢痕改善率.结果 与对照侧瘢痕相比,观察侧SCAR量表的瘢痕扩展宽度、红斑、肥厚/萎缩和总分得分显著降低,差异有统计学意义(P<0.05).观察侧瘢痕改善率明显优于对照侧,差异有统计学意义(P<0.05).结论 低浓度5-Fu注射联合点阵激光在增生性瘢痕早期干预中,治疗效果优于单独应用5-Fu治疗.
嵌甲是临床常见的手足疾病,主要是指甲修剪不当、长期挤压、摩擦等导致甲板外侧缘嵌入甲周组织,加之足部卫生状况差,引起甲周组织充血、水肿、疼痛,形成嵌甲型甲沟炎[1-2].临床治疗嵌甲性甲沟炎主要根据患者情况,予以保守治疗、局部切除或拔甲为主[3],但治疗不彻底,易反复,治疗周期长,严重影响患者治疗体验.由于局部切除或拔甲术治疗嵌甲性甲沟炎存在诸多不足,本研究以Winograd术式为基础,结合临床治疗经验,对患者行改良嵌甲切除术,并结合垂直半褥式缝合甲沟重建术,二者联合治疗嵌甲性甲沟炎,取得较好的临床疗效,报道如下.
目的 观察调Q开关激光联合艺霏氨甲环酸巴布贴治疗对黄褐斑的临床疗效.方法 选择浙江萧山医院整形美容激光中心68例黄褐斑患者,按照入院顺序分成对照组和试验组,对照组予调Q开关激光治疗,1个月治疗1次,共治疗8次,试验组在此基础上联合艺霏氨甲环酸巴布贴外用治疗,8个月治疗,每周3次,共治疗96次.治疗结束后比较2组临床疗效.结果 试验组基本治愈15例,显效12例,好转6例,无效1例,对照组基本治愈7例,显效16例,好转8例,无效3例,2组临床疗效相比较,差异有统计学意义(P＜0.05).结论 调Q开关激光治疗联合艺霏氨甲环酸巴布贴对黄褐斑效果明显,联合治疗方案值得推广.
Objective To investigate the clinical efficacy and safety of TAPP surgery in inguinal hernia repair for the elderly by different mesh materials.Methods 24 elderly patients (32 cases) with inguinal hernia were randomly divided into the two groups.The control group was repaired by the braid mesh,while the other 16 cases in the observation group were repaired by the three dimension mesh.The operative indicators,the VAS pain score,the incidence rate of complications and recrudesce rate were compared between the two groups.Results The operative time,time length of staying and costs of the two groups were close,no statistical differences were found (all P ＞ 0.05) ; VAS scores of postoperative 24h,48h,72h of the observation group were (0.97 ± 0.76) points,(0.62 ± 0.55) points,(0.49 ± 0.37) points respectively,which were significantly lower than those of the control group (t =6.331,6.031,5.882,all P ＜0.05).The incidence rate of complication in control group was 16.67％ (2/12),no statistical difference was found between two groups (x2 =0.000,P ＞ 0.05).No recurrence was found in two groups.Conclusion The three dimension mesh and the braid mesh in TAPP are safe and effective,the three dimension mesh has better performance in pain.
目的：探讨左肝外叶切除术联合胆道镜针对左肝内胆管结石治疗的疗效性和安全性。方法入选2008年2月至2013年6月，于本院同期分别进行两种手术的左肝内胆管结石患者共计36例，观察组19例，采用左肝外叶切除术联合胆道镜取石手术；对照组17例，采用左肝外叶切除术，对比研究两组患者术中、术后疗效和并发症发生情况。结果观察组手术时间明显短于对照组，术中出血量明显少于对照组，差异具有统计学意义（P＜0.05）；观察组并发症发生率为21.05%，明显低于对照组41.18%，差异显著（P＜0.05）；两组患者随访率差异无显著性（P＞0.05），观察组残石率为0，对照组残石率为11.76%，组间差异显著（P＜0.05）。结论左肝外叶切除术联合胆道镜治疗左肝内胆管结石疗效突出，能够有效缩短手术时间，降低术中出血量，减轻患者的经济负担，同时能够有效降低并发症发生率和残石率，值得临床推广应用。
目的：观察Dufourmentel 改良菱形皮瓣成形术修复皮肤缺损355例临床效果及并发症情况。方法应用Dufourmentel改良菱形皮瓣成形术修复皮肤缺损。结果术后切口感染导致皮瓣部分坏死4处，切口愈合不良2处，其余存活良好，并发症发生率仅1.6%。全部创面得以 I 期修复，经随访2个月至1年，切口瘢痕不明显，无明显继发猫耳畸形，局部外形满意。结论 Dufourmentel改良菱形皮瓣修复皮肤缺损有血运可靠、设计灵活、简单易学、成功率高、切口瘢痕不明显等优点。
