成簇的规律间隔的短回文重复序列及其相关蛋白(CRISPR-Cas)系统是一种存在于大多数细菌和古菌中的"获得性免疫系统",已被广泛应用于生物、医学等多个研究领域.传统的Cas核酸酶(Cas9、Cas12和Cas13)因为分子尺寸较大,将基因编辑工具精确送到真核细胞内受到阻碍.研究发现,Cas12f是目前已知的最小RNA引导核酸酶,有着与众不同的属性:除分子比较小外;对单链DNA切割不需要靶标前间区序列邻近基序(Protospacer adjacent motif,PAM);非常长的(153～169个核苷酸)反式激活crRNA(tracrRNA);结合单拷贝向导RNA(gRNA)后的二聚化;不对称的双链切割模式;针对单链DNA的附带切割活性;对识别序列中间的碱基要求严格等.基于目前研究,Cas12f具体作用机制尚不明确,本文对近年来CRISPR-Cas12f起源、分类、作用机制及其在基因编辑领域中应用的研究进行综述,以期开发微型Cas12f精准基因编辑和治疗工具,同时为相关研究领域的科研工作提供参考.

蛋白质与酶工程作为高等院校生物技术和生物工程专业人才培养的核心课程之一,立足拓展学生的思维能力、实际问题的解决能力和全球变革下的综合视野.通过在蛋白质与酶工程课程教学的过程中引入OBE教育理念,对教学模式、课程体系、评价机制、课程思政等方面进行改革,从而提高教学质量、活化学生创新实践能力、满足社会企业的岗位需求,落实"立德树人"教育目标.期末"自我能力评价"问卷调查结果显示,学生对该门课程的混合式教学模式认同率超过 80%,对自身能力提升评价在 80 分以上的学生超过 50%.因此,基于OBE理念的蛋白质与酶工程课程教学改革在培养学生开拓创新方面具有积极的促进作用,为适应新时代复合应用型人才培养体系的课程教学改革提供必要的参考.

随着经济的发展和社会的进步，健康的观念不断普及，人们对健康的诉求和美好向往日益强烈。现实对医学的发展提出了更高的要求，医学人才的培养是满足人民日益增长的健康服务需求的重要工作，而提高医学生的医德素质是医学院校面临的重要课题。本文分析了目前医学生在职业精神形成过程中存在的几点问题，并对解决医德视域下医学生职业精神弱化的情况进行了有益的探索。

大健康背景下,人民日益增长的健康服务需求促进医学院校开展"新医科"建设.现代产业学院作为产学研融合的教育载体是医学院校建设"新医科"的重要抓手.本文阐述了新医科背景下现代产业学院建设的契机和思路,总结了现代产业学院产学研融合人才培养路径的实践,为医学院校建设"新医科"提供有益参考.

Achieving the Healthy China 2030 goal requires a large number of medical professionals.Medical universities are an important guarantee for the supply of healthy Chinese talents.The popular-ization of the concept of the"great health",the concept of preventive treatment has received more and more attention and recognition,and the people pay more attention to the monitoring and prevention of diseases,so cultivating innovative and compound medical laboratory talents is one of the important topics of medical universities.After more than half a century of development,China's laboratory medicine has moved from the initial manual operation to the new technology platform and assembly line operation,and the training of medical laboratory talents has been reformed to keep up with the health needs of the people.The Healthy China 2030 construction goal puts forward new requirements for medical colleges and universities to cultivate medical laboratory talents in the new era.Under the background of new medical sciences,cultivating innovative and compound medical talents to meet people's health needs is an important direction for medical schools to cultivate talents.The educational resource integration platform is an important starting point for medical colleges and universities to achieve the goal of"Medicine+X"medical talent training;Local medical colleges and universities actively promote the reform of medical talent training according to the actual conditions of running schools,and providing medical talents suitable for the development of local health undertakings is one of the important ways for medical colleges and universities to serve the people's health cause.This paper expounds the policy orientation of medical laboratory talent training and the demand orientation of medical laboratory talent training,summarizes the reform and practice of medical laboratory talent training mode,and provides a useful reference for the training mode of innovative laboratory professionals based on the medical colleges of modern industrial colleges under the background of"new medical science".

2018年,国务院印发《关于推动创新创业高质量发展打造"双创"升级版的意见》中明确表示要强化大学生的创新创业教育培训,把创新创业教育和实践课程纳入高校必修课体系[1-3].2021年10月,国务院办公厅印发《关于进一步支持大学生创新创业的指导意见》中强调要深化高校创新创业教育改革,将创新创业教育贯穿人才培养全过程,建立以创新创业为导向的新型人才培养模式[4].

体外诊断(in vitro diagnosis,IVD)产业发展迅猛,我国IVD每年复合增长率保持在15％～20％,增速明显高于全球平均水平,IVD企业急需大量医学检验体外诊断复合型人才.然而,近40多年来,我国高校医学检验专业办学模式、培养目标及课程体系设置等变化不大,培养目标单一,课程设置同质化严重,医学检验专业人才培养与岗位需求严重脱节.为培养体外诊断产业复合型医学检验人才,以行业发展和岗位需求为导向,我院设置了"体外诊断产业"特色拓展方向,与体外诊断企业共同成立"体外诊断现代产业学院",修订了人才培养方案,优化了课程体系,主编了相应课程创新特色教材,组织牵头全国高校医学检验专业毕业生体外诊断企业在线招聘会,效果显著.

An easy prepared probe, BHMMP, was designed and synthesized, which displayed a significant fluorescence enhancement (over 38-fold) and obvious color change in the recognition of Al3+. The binding ratio of probe BHMMP to Al3+ was determined as 1:1, according to Job plot. The binding mechanism was fully clarified by the experiments, such as FT-IR spectrum, ESI–MS analysis, and 1H NMR titration. A DFT study further confirmed the binding mode of BHMMP to Al3+. The limit of detection (LOD) for Al3+ was determined as low as 0.70 µM, based on the fluorescence titration of BHMMP. Moreover, the results from real sample experiments, including real water samples, test papers, and cell images, well-demonstrated that BHMMP was capable of sensing Al3+ in environmental and biological systems.

文章选取右江民族医学院2019级卫生检验与检疫专业52名本科生和2018级卫生检验与检疫专业50名本科生分别作为实验组和对照组,并采用雨课堂联合TBL教学模式和传统教学模式分别进行教学,以此研究雨课堂联合TBL教学模式在"卫生检疫学"课程教学中的应用效果,结果发现,实验组学生课后测验成绩高于对照组学生,且实验组学生满意本次课堂互动效果的人数多于对照组学生,可见,雨课堂联合TBL教学模式应用效果较好.