Soil salinity severely constrains strawberry production by disrupting ion homeostasis and provoking oxidative injury. This study investigated whether soluble silicon (Si) and activated carbon (AC) act to enhance salt tolerance in strawberry (Fragaria × ananassa). Under NaCl stress, plants showed pronounced growth inhibition, increased Na+ accumulation and a deteriorated K+/Na+ balance, accompanied by elevated reactive oxygen species (ROS) and lipid peroxidation. In contrast, combined AC + Si treatment consistently provided the strongest protection, improving seedling vigor and survival. Relative to NaCl alone, AC + Si increased shoot and root fresh weight by 67.5% and 78.5%, reduced shoot Na+ by 59.1%, and lowered shoot H2O2 and MDA by 62.6% and 66.5%, respectively, indicating marked improvement in ion–redox homeostasis. Beyond plant responses, AC-containing treatments alleviated salt-induced increases in soil electrical conductivity, coinciding with a clear restructuring of the rhizosphere bacterial community and enrichment of putatively beneficial taxa. Transcriptome profiling further supported coordinated reprogramming of ion transport, redox control and stress-responsive signaling pathways under the AC + Si regime. Collectively, the results indicated that Si and AC co-application enhances strawberry salt tolerance through an integrated soil–plant–microbiome mechanism that stabilizes ion homeostasis and reinforces redox homeostasis.

HD-ZIP proteins play multifaceted roles in plant development and stress responses. In this study, FaANL2 was found to respond to light stimuli and phytohormones, including abscisic acid (ABA) and auxin, which in turn negatively correlate with anthocyanin accumulation in strawberry fruit (Fragaria × ananassa). In strawberry fruit development and ripening process, the transcriptions of FaANL2 were attenuated and correlating with increased anthocyanin content. Dual-luciferase assays indicated that FaANL2 significantly repressed the promoter activity of FaMYB10, the master activator of anthocyanin biosynthesis. Yeast one-hybrid and electrophoretic mobility shift assays showed that FaANL2 could directly bind to the promoter of FaMYB10. Transient overexpression of FaANL2 in strawberry fruit significantly decreased FaMYB10 expression and anthocyanin production. In contrast, transient RNAi of FaANL2 resulted in increases in FaMYB10 expression and anthocyanin content. These results demonstrated that FaANL2, which is influenced by light and phytohormones, suppressed anthocyanin accumulation in strawberry via direct downregulation of FaMYB10.

Research on the ripening of fleshy fruits has relied on techniques that measure transcriptional changes. How ripening is linked to posttranslational modifications such as protein phosphorylation remains less studied. Here, we characterize the MADS-box SEPALLATA 4 (SEP4) subfamily transcription factor FaCMB1, a key negative regulator controlling strawberry ripening, whose transcript and protein abundance decrease progressively with fruit development and are repressed by abscisic acid (ABA). Transient RNAi or overexpression of FaCMB1 significantly altered the fruit ripening process and affected the content of endogenous ABA and ripening-related quality. Transcriptome sequencing (RNA-seq) analysis suggested that manipulation of FaCMB1 expression levels affected the transcription of FaASR (ABA-, stress-, ripening-induced), while FaCMB1 can repress the gene expression of FaASR by directly binding to its promoter. Furthermore, FaASR inhibited the transcriptional activity of FaCYP707A4, a key ABA 8'-hydroxylase enzyme involved in ABA catabolism. We show that FaCMB1 can be phosphorylated by the kinase FaSTPK, and Phos-tag assays indicated that the phosphorylation level of FaCMB1 increases during fruit ripening. This phosphorylation of FaCMB1 affects the binding ability of FaCMB1 to the FaASR promoter and alleviates its transcriptional repression. In conclusion, we elucidated a feedback regulatory path involving FaCMB1-FaASR-FaCYP707A4-ABA. During the fruit ripening process, an increase in ABA content led to a decrease in FaCMB1 transcript and protein levels, which, combined with increased phosphorylation levels, collectively impaired the transcriptional repression of FaASR by FaCMB1. Meanwhile, the increased transcriptional level of FaASR further repressed the expression level of FaCYP707A4, leading to ABA accumulation and fruit ripening.

Genome assembly and structural variation analysis of strawberry varieties are essential for understanding the genetic basis of fruit quality traits, such as fruit texture, organic acid content and aroma. In this study, we employed PacBio HiFi reads and Hi-C sequencing to generate a haplotype-resolved chromosome-level genome assembly of the improved strawberry cultivar 'Yuexin', which was selected from the crossing of '0362' ('Camarosa' × 'Akihime') × 'Sachinoka'. The assembly sizes of the primary assembly and two haplotypes were 875.84 Mb, 867.93 Mb and 823.17 Mb, with N50 length of 27.6 Mb, 27.3 Mb and 27.6 Mb respectively. Comprehensive genome comparison with its parent, cultivar 'Camarosa', identified numerous structural variants, which are positioned in the promoter or gene body regions. Some of these genes are involved in pathways related to cell wall, malate metabolism and fruit aroma. This dataset comprises the assembled genome sequence, annotations, and identified structural variants, providing new insights into the genetic basis of improved fruit quality.

Phytohormones, epigenetic regulation and environmental factors regulate fruit ripening but their interplay during strawberry fruit ripening remains to be determined. In this study, bagged strawberry fruit exhibited delayed ripening compared with fruit grown in normal light, correlating with reduced abscisic acid (ABA) accumulation. Transcription of the key ABA catabolism gene, ABA 8'-hydroxylase FaCYP707A4, was induced in bagged fruit. With light exclusion whole genome DNA methylation levels were up-regulated, corresponding to a delayed ripening process, while DNA methylation levels in the promoter of FaCYP707A4 were suppressed, correlating with increases in transcript and decreased ABA content. Experiments indicated FaCRY1, a blue light receptor repressed in bagged fruit and FaAGO4, a key protein involved in RNA-directed DNA methylation, could bind to the promoter of FaCYP707A4. The interaction between FaCRY1 and FaAGO4, and an increased enrichment of FaAGO4 directed to the FaCYP707A4 promoter in fruit grown under light suggests FaCRY1 may influence FaAGO4 to modulate the DNA methylation status of the FaCYP707A4 promoter. Furthermore, transient overexpression of FaCRY1, or an increase in FaCRY1 transcription by blue light treatment, increases the methylation level of the FaCYP707A4 promoter, while transient RNA interference of FaCRY1 displayed opposite phenotypes. These findings reveal a mechanism by which DNA methylation influences ABA catabolism, and participates in light-mediated strawberry ripening.

Cultivated strawberry (Fragaria x ananassa) is a popular, economically important fruit. The ripening of the receptacle (pseudocarp), the main edible part, depends on endogenously produced abscisic acid (ABA) and is suppressed by the high level of auxin produced from achenes (true fruit) during early development. However, the mechanism whereby auxin regulates receptacle ripening through inhibiting ABA biosynthesis remains unclear. Here, we identified AUXIN RESPONSE FACTOR 2 (FaARF2), which showed decreased expression with reduced auxin content in the receptacle, leading to increased ABA levels and accelerated ripening. Dual-luciferase, yeast one-hybrid, and electrophoretic mobility shift assays demonstrated that FaARF2 could bind to the AuxRE element in the promoter of 9-CIS-EPOXYCAROT-ENOID DIOXYGENASE 1 (FaNCED1), a key ABA biosynthetic gene, to suppress its transcriptional activity. Transiently overexpressing FaARF2 in the receptacles decreased FaNCED1 expression and ABA levels, resulting in inhibition of receptacle ripening and of development of quality attributes, such as pigmentation, aroma, and sweetness. This inhibition caused by overexpressing FaARF2 was partially recovered by the injection of exogenous ABA; conversely, transient silencing of FaARF2 using RNA interference produced the opposite results. The negative targeting of FaNCED1 by FaARF2 is a key link between auxin-ABA interactions and regulation of strawberry ripening.

为明确氟酰羟·咯菌腈对草莓灰霉病的防治效果,以啶菌噁唑、 嘧环·咯菌腈、 唑醚·氟酰胺为对照药剂,开展田间试验.灰霉病始发期,4种杀菌剂各按一定浓度喷施,隔7 d二次防治.结果表明,400 g·L-1氟酰羟·咯菌腈悬浮剂对草莓灰霉病防治效果佳,随浓度降低,防治效果下降,差异显著,建议田间施用浓度为750倍～1000倍;25％啶菌噁唑悬浮剂750倍防治效果与400 g·L-1氟酰羟·咯菌腈悬浮剂1000倍相近,差异不显著;62％嘧环·咯菌腈悬浮剂与42.4％唑醚·氟酰胺悬浮剂防治效果较差.因此,可用400 g·L-1氟酰羟·咯菌腈悬浮剂,与25％啶菌噁唑悬浮剂轮换防治草莓灰霉病,以延缓抗药性的产生.

近年来浙江省草莓品种结构已呈现出向多元化发展的鲜明特点,一批自主选育的优质抗病品种在生产上显示出较高经济效益.为丰富浙东地区设施草莓品种,满足消费者的不同需求,提高种植户的经济效益,本研究主要通过总结引自浙江省农业科学院的草莓新品种越心、越秀、越可(建德红)在浙东地区的栽培技术,以期为新品种推广和莓农生产提供参考依据.

以越秀、 越心、 红颜3个草莓设施栽培品种为试材,选择LED格栅灯(R:B=5:2)作为补光光源,探讨LED组合光源对设施栽培草莓营养生长和果实品质的影响.结果表明,LED补光能增强草莓植株营养生长、促进光合作用,株高、叶长、叶宽、叶绿素含量、净光合速率和蒸腾速率等指标显著提高.补光同样有利于提高设施草莓产量和改善果实外观和内在品质.3个供试草莓品种中,越心对LED补光反应有所不同,在产量和着色上较对照表现出差异,但植株形态、果实品质则没有显著变化.LED补光对于改善设施光环境,增强长势、提高产量和改善果实综合品质有较好效果,在产业上有推广应用前景.

Nanoplastics (NPs) have emerged as a novel environmental threat due to their potential impacts on both animals and plants. Currently, research on the ecotoxicity of NPs has mainly focused on marine aquatic organisms and freshwater algae, with very limited investigations conducted on horticultural plants. This study examined the effects of varying concentrations (0, 1, 10, 50 mg & BULL;L-1) of polystyrene NPs (PS-NPs) on strawberry growth. The findings revealed that low concentrations of PS-NPs stimulated strawberry growth, whereas high concentrations impeded it. Notably, diverse strawberry cultivars displayed considerable differences in their sensitivity to PS-NP exposure. Laser scanning confocal microscopy confirmed the absorption of PS-NPs by strawberry roots, with variations in PS-NP accumulation observed across different cultivars. Comparative transcriptomics analysis suggested that the differential expression of genes responsible for calcium ion transport played a significant role in the observed intervarietal differences in PS-NP accumulation among strawberry cultivars. Furthermore, distinct variations in endogenous oxidative responses were observed in different strawberry cultivars under PS-NP treatment. Further analysis indicated that the down-regulation of peroxidase (POD) gene expression and terpenoid compounds accumulation were responsible for heightened endogenous oxidative stress observed in certain strawberry cultivars under PS-NP treatment. Transcriptomic and metabolomic analyses were performed on six strawberry cultivars to investigate their response to PS-NPs in terms of endogenous gene expression and metabolite accumulation. The results identified one commonly up-regulated gene (wall associated receptor kinase-like) and sixteen commonly down-regulated genes associated with lipid metabolism and carbohydrate metabolism. In addition, a significant reduction in fatty acid metabolite accumulation was observed in the six strawberry cultivars under PS-NP treatment. These findings have significant implications for understanding the effects of NPs on strawberry growth, metabolism, and antioxidant responses, as well as identifying marker genes for monitoring and evaluating the impact of NP pollution on strawberry.

Recently, increasing evidence suggests that DNA methylation plays a crucial role in fruit ripening. However, the role of DNA methylation in regulating specific traits, such as flavor, remains unclear. Here, we report a role of DNA methylation in affecting furanone biosynthesis in strawberry. Strawberry quinone oxidoreductase (FaQR) is a key enzyme in furanone biosynthesis. There are four FaQR homologs in strawberry cultivar 'Yuexin', and one of them, FaQR3, contributes ~50% of FaQR transcripts, indicating a major role of FaQR3 in furanone biosynthesis. Through characterization of levels of DNA methylation and FaQR3 transcript and furanone contents during fruit ripening and after the application of DNA methylation inhibitor, we found that the DNA methylation level of the FaQR3 promoter was negatively correlated with FaQR3 expression and furanone accumulation, suggesting that DNA methylation may be involved in furanone biosynthesis through adjusting FaQR3 expression, and responded to different temperatures consistently. In addition, transient expression of a gene in the RNA-directed DNA methylation (RdDM) pathway, FaAGO4, and enrichment analysis of the 24-nucleotide siRNAs suggested that DNA methylation in the FaQR3 promoter is mediated by the RdDM pathway. Transient RNA interference (RNAi) of FaDML indicated that the demethylation pathway may be involved in regulating furanone accumulation. These findings provide new insights into the role of DNA methylation and demethylation in affecting flavor quality in strawberry during fruit ripening.

以"越秀"草莓为试材,探究了穴盘苗和裸根苗两种育苗方式对草莓定植成活率及生长结果的影响.结果表明,穴盘苗定植后成活率达到99.83％,比裸根苗提高13.87％,缓苗期短、长势强.定植后46 d(裸根苗现蕾期),穴盘苗长6片新叶,生长速率R=0.13;裸根苗长4片新叶,R=0.09.穴盘苗第1花序花数17.14朵,各花序总花数37朵,第1花序花数比率45.94％,分别比裸根苗提高30.84％、16.35％、11.53％.穴盘苗单株前期产量204.99 g,总产量549.93 g,前期产量比率37.28％,分别比裸根苗提高15.79％、4.73％、11.62％;穴盘苗总销售额41 987元/667 m2,比裸根苗多1 051元/667 m2;穴盘苗单果质量降低,平均单果质量14.86 g,大、中、商品果率为17.09％、17.40％、58.66％,分别比裸根苗低 9.99％、12.36％、9.80％、4.51％;小果率 24.17％,比裸根苗高6.76％.9-10月中旬高温天气不利于花芽分化,穴盘苗定植后第1花序物候期较裸根苗不一致,虽然现蕾期早,但部分植株花芽分化延迟,导致花期和果实成熟期均推迟.因此,若遇高温天气,穴盘苗可适当延迟定植.

To explore effects of microbial agents on strawberry（Fragaria × ananassa Duch.） growth and soil properties, three treatments were set up in this study: without microbial agent（CK）, with single microbial agent（T1） and with compound microbial agent（T2）. Strawberry survival rate and yield were calculated, and soil samples were collected to measure soil physico chemical traits and microbial community structure. The results showed that application of microbial agents significantly improved the growth and yield of strawberry.Compared with CK, the treatments of T1 and T2 increased the strawberry survival rate by 17. 99 and 25. 19percentage points, respectively, and increased strawberry yield by 30. 16% and 41. 79%, respectively. The microbial agents significantly improved soil fertility. Compared with CK, T2 treatment significantly increased soil available phosphorus and available potassium content by 18. 31% and 55. 27%, respectively. However, microbial agents enhanced soil electrical conductivity and lowered soil pH, which indicates long-term application of microbial agent may raise the risk of soil salinization and acidification. The results of microbial community structure showed that Firmicutes and Ascomycota were the dominant bacteria and fungi at phylum level, and Bacillus and Aspergillus were the dominant bacteria and fungi at genus level. Both two types of microbial agents increased the richness and diversity of soil fungal community, but had a negligible effect on the soil bacterial community. Compared with CK, the microbial agents increased the relative abundance of Bacillus, Rhizopus and Pseudallescheria, in which the relative abundance of Rhizopus and Pseudallescheria reached a significant level in T2 treatment. The microbial agents decreased the relative abundance of Sphingomonas, Aspergillus and Penicillium, while the relative abundances of Aspergillus and Penicillium were significantly decreased by 14. 62 and 1. 79 percentage points in T2 treatment, respectively. The results of redundancy analysis show that microbial agents, especially compound microbial agents, can significantly improve soil physico chemical properties and microbial community, which is beneficial to reduce the occurrence of diseases and increase the yield of strawberry. This study provided biocontrol materials and theoretical basis for the sustainable development of the strawberry industry.

Cultivated strawberry (Fragaria × ananassa), a world-famous fruit, is subjected to rapid softening during ripening, resulting in a shorter shelf life and severe economic losses during storage and transportation. However, there is limited understanding of the molecular mechanism underlying differences in fruit firmness during ripening and postharvest among cultivated strawberries. Here, we explored this molecular mechanism by comparing three cultivated strawberries via firmness measurement, transcriptome analysis, quantitative real-time polymerase chain reaction, and correlation analysis, and revealed FaEXP7, FaPG2, FaPLA, and Faβ-Gal4 as potential softening activators expressed before harvest to determine fruit with more softened texture and shorter shelf life, and that extremely high expression levels of FaCEL1-1 and FaCEL1-3 during ripening might be accelerators to intensify this situation. Additionally, both the enzyme activities of FaCEL and the expression pattern of FaCEL1-3 showed a significantly negative correlation with fruit firmness after harvest, suggesting that FaCEL1-3 might play a key role in promoting strawberry fruit softening not only during ripening but also postharvest. These results showed that the difference in fruit firmness and shelf life among cultivated strawberries was controlled by the temporal expression pattern of a legion of cell wall-associated genes during ripening and postharvest.

为明确智利小植绥螨、20%异噁唑虫酰胺悬浮剂、30%乙唑螨腈悬浮剂对设施栽培草莓上二斑叶螨的控制作用,本研究开展生物防治与化学防治试验.结果表明,智利小植绥螨按益害比 1 ∶ 17 释放,能够有效控制草莓二斑叶螨,速效性低、持效期长,释放后 14 d防治效果明显上升,后期智利小植绥螨与二斑叶螨种群数量达到动态平衡.20%异噁唑虫酰胺悬浮剂对草莓二斑叶螨防治效果佳,速效性与持效性均较优,2 000、3 000、5 000 倍处理的防治效果差异不显著,药后 28 d的防治效果均在 99%以上,建议田间防治时用 5 000 倍即可.30%乙唑螨腈悬浮剂对草莓二斑叶螨防治效果低,二斑叶螨已对其产生抗性.20%异噁唑虫酰胺悬浮剂与30%乙唑螨腈悬浮剂对智利小植绥螨均不安全,与该捕食螨应分开使用.

As a canonical non-climacteric fruit, strawberry (Fragaria spp.) ripening is mainly mediated by abscisic acid (ABA), which involves multiple other phytohormone signalings. Many details of these complex associations are not well understood. We present an coexpression network, involving ABA and other phytohormone signalings, based on weighted gene coexpression network analysis of spatiotemporally resolved transcriptome data and phenotypic changes of strawberry receptacles during development and following various treatments. This coexpression network consists of 18,998 transcripts and includes transcripts related to phytohormone signaling pathways, MADS and NAC family transcription factors and biosynthetic pathways associated with fruit quality. Members of eight phytohormone signaling pathways are predicted to participate in ripening and fruit quality attributes mediated by ABA, of which 43 transcripts were screened to consist of the hub phytohormone signalings. In addition to using several genes reported from previous studies to verify the reliability and accuracy of this network, we explored the role of two hub signalings, small auxin up-regulated RNA 1 and 2 in receptacle ripening mediated by ABA, which are also predicted to contribute to fruit quality. These results and publicly accessible datasets provide a valuable resource to elucidate ripening and quality formation mediated by ABA and involves multiple other phytohormone signalings in strawberry receptacle and serve as a model for other non-climacteric fruits.

为明确越秀草莓高冷地苗在浙江省临海市的栽植表现,以高冷地苗和本地苗为试材,分别于9月4日和9月20日定植,移栽当日进行花芽分化镜检,移栽后持续调查植株长势、物候期、单株产量和果实品质.结果表明,9月4日和9月20日,高冷地苗分别处于花序分化期和雌蕊形成期,本地苗分别处于花芽未分化期和花芽分化始期.9月4日定植的高冷地苗长4.0片新叶后进入现蕾期,定植后66 d进入初熟期、69 d进入成熟期,顶花序果实在11月中旬大量上市,但1-2月断档期长达58 d,前期产量占比不足1/3..9月20日定植的高冷地苗长3.0片新叶后进入现蕾期,定植后60 d进入初熟期、63 d进入成熟期,顶花序果实在11月下旬大量上市,1-2月断档期长达45d,顶花序花数少,前期产量较低.9月4日定植的本地苗植株间物候期不一致,长7.0片新叶后进入现蕾期,果实3月以后才大量上市,且顶花序和第一腋花序集中生长,导致果实小、品质差、商品果率低、总产量低.9月20日定植的本地苗长5.5片新叶后进入现蕾期,定植后86 d进入初熟期、97 d进入成熟期,顶花序果实在12月下旬大量上市,前期产量占44.57％,2月有17 d的断档期.因此,越秀高冷地苗宜在7月下旬扦插,培养成茎基部粗度1cm左右的大苗,9月上旬在浙江省临海市定植,搭配本地苗9月下旬定植,能够避免集中采收,均衡市场供应,提高经济效益.

Background Colletotrichum gloeosporioides, a soil-borne fungal pathogen, causes significant yield losses in many plants, including cultivated strawberry (Fragaria × ananassa, 2n = 8x = 56). Thaumatin-like proteins (TLPs) are a large and complex family of proteins that play a vital role in plant host defense and other physiological processes. Methods To enhance our understanding of the antifungal activity of F. × ananassa TLPs (FaTLP), we investigated the genome-wide identification of FaTLP gene families and their expression patterns in F. × ananassa plants upon pathogen infection. Moreover, we used RNA sequencing (RNA-seq) to detect the differences in the expression patterns of TLP genes between different resistant strawberry cultivars in response to C. gloeosporioides infection. Results In total, 76 TLP genes were identified from the octoploid cultivated strawberry genome with a mean length of 1,439 bp. They were distributed on 24 F. × ananassa chromosomes. The FaTLP family was then divided into ten groups (Group I–X) according to the comparative phylogenetic results. Group VIII contained the highest number of TLP family genes. qRT-PCR analysis results indicated that FaTLP40, FaTLP41, FaTLP43, FaTLP68, and FaTLP75 were upregulated following C. gloeosporioides infection in the resistant octoploid strawberry. Conclusions The data showed some differences in TLP gene expression patterns across different resistant strawberry cultivars, as well as faster TLP defense responses to pathogenic fungi in resistant cultivars. This study will aid in the characterization of TLP gene family members found in octoploid strawberries and their potential biological functions in plants’ defenses against pathogenic fungi.

为了解基质栽培和土壤栽培条件下草莓果实品质形成的差异,探究其影响规律,以越心草莓为材料,测定果实不同发育时期的蔗糖和柠檬酸含量,以及相关基因的表达量.结果显示,土壤栽培条件下蔗糖和柠檬酸含量高于基质栽培,其中,蔗糖积累的差异主要体现在草莓果实的尖部,柠檬酸积累的差异在果实尖部和基部.FaSPS2基因是土壤栽培和基质栽培蔗糖积累差异的关键基因,与蔗糖积累呈正相关.FaACO1、FaACO3基因的表达量与柠檬酸的积累呈负相关,基质栽培中FaACO1、FaACO3的表达量显著高于土壤栽培,导致基质栽培的成熟草莓果实中的柠檬酸含量低于土壤栽培.因此,FaSPS2、FaACO1和FaACO3基因在越心草莓蔗糖和柠檬酸含量的积累中发挥了重要作用,土壤栽培较基质栽培可能更有利于越心草莓蔗糖和柠檬酸含量积累.

基于基因组序列信息,研究了二倍体森林草莓和八倍体栽培草莓染色体上SSR位点的分布特点.利用AutoSSR软件在森林草莓和栽培草莓中分别发掘到153 826个和329 801个SSR位点,发生频率分别为1.43 kb/SSR和2.44 kb/SSR.SSR重复基序为单核苷酸的数量最多,分别占总数的40.92％和38.94％,其次为二核苷酸类型,分别占22.79％和20.04％.在二倍体草莓和八倍体草莓中分别鉴定到454和479种SSR重复类型,其中A/T重复类型的SSR出现的频率最高,分别占总SSR位点的39.45％和37.08％.二核苷酸重复类型中,二倍体草莓的AT/AT占比最高,为11.61％,其次为AG/CT(8.74％);八倍体草莓中AG/CT重复类型占比最高(9.94％),其次为AT/AT(7.35％).用Beacon Designer软件设计SSR引物,随机挑选了59对SSR引物在77份草莓材料中进行验证分析.研究结果显示,59对SSR引物共扩增出254个多态性位点,平均扩增4.31个位点,位点的平均多态信息量(PIC)为0.26,介于0.07～0.97之间,其中35个SSR位点PIC≥0.50,为高多态性位点,聚类分析显示品种间的相似系数范围为0.47～0.99.该研究为草莓种质资源的遗传多样性评价、变异分析、分子标记辅助育种等工作提供了技术支持.