随着社会经济和制药工业的快速发展,医疗服务的需求日益增加.高校要紧跟医药市场步伐,培养综合性高质量药学人才.为提高药学专业办学水平,在药学专业认证背景下进行教学质量现状分析,立足于高校的办学特点和培养目标,从课程体系、课堂教学、毕业论文、实习实训、师资队伍等方面进行探究和改革,培养高质量的药学专业人才.

In order to investigate the relationship between antioxidant efficacy and active components of Smilax riparia,the HPLC fingerprint of S.riparia extracts were determined,similarity evaluation and cluster analysis were carried out,while analysis software was used for PLSR to establish the spectra effect relationship,and validation test of in vitro anti-oxidation was carried out.The results were as follows:(1)HPLC fingerprints of 13 batches of S.riparia with 14 main common peaks were established.(2)Thirteen batches of S.riparia samples were grouped into two categories,the samples of S.riparia with close geographical location were clustered into one group,and the chemical fingerprints of S.riparia with close geographical location were highly similar.(3)PLSR showed that the areas of peaks 1,2,3,5,6,9 and 14 in the fingerprint were positively correlated with the antioxidant effect,while the areas of peaks 4,7,8,10,11 and 12 were negatively correlated with the antioxidant effect,the VIP values of peaks 9,11,3,4 and 5 were all greater than 1.(4)Peak 9 was oleanolic acid,Peak 10 was ursolic acid,and Peak 9 had the largest scavenging capacity for ABTS free radicals.Therefore,antioxidant efficacy of S.riparia is contributed by a combined action of multi-components rather than one component,and Peak 9(oleanolic acid)may be the material basis of the antioxidation effect of S.riparia.

Sixteen pentacyclic triterpenoids were isolated from Rubus pirifolius Smith by phytochemical methods and subdivided into one new ursane-type triterpenoid, 23-O-galloyltormentic acid (1), and fifteen known pentacyclic triterpenoids (2–16). Their structures were determined by spectroscopic techniques. All these compounds were isolated from R. pirifolius for the first time. The chemophenetic significance of triterpenoids for the genus Rubus is also discussed here briefly.

Introduction: Determining the six components of Dalbergia Benthamii Prain. using quantitative analysis of multi- components by a single marker (QAMS). Materials and methods: A HPLC method was developed as QAMS to determine glycyrrhizic acid, robustic acid, 4'-hydroxy5,7-dimethoxy-6-( 3-methyl-2-butenyl)-isoflavone, robustin methyl ether, methyl robustate, and 18 alpha- glycyrrhetinic acid in Dalbergia Benthamii Prain. Glycyrrhizic acid was selected as the internal reference substance, and the relative correction factors of robustic acid, 4'-hydroxy-5,7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone, robustin methyl ether, methyl robustate, and 18 alpha-glycyrrhetinic acid were calculated. The contents of these six active components were determined using the external standard method and QAMS. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Results: The RCFs of robustic acid, 4'-hydroxy-5,7-dimethoxy-6-(3-methyl-2-butenyl)-isoflavone, robustin methyl ether, methyl robustate, and 18 alpha-glycyrrhetinic acid were 0.1651, 0.1507, 0.3662, 0.1956, and 0.6013, respectively, indicating good reproducibility. There was no significant difference between the results of the external standard method and QAMS. Conclusion: The proposed QAMS method was accurate and feasible according to the methodological experiments, which can be used to evaluate the quality of Dalbergia Benthamii Prain.

现行的培养模式要求强化创新型人才的培养,高校实验室对创新型人才的培养至关重要,是培养和提高学生实践能力和创新能力的重要场所.该文基于创新人才培养的管理理念、管理方式和管理制度,探讨通过加强实验室管理和建设,加强实验室师资队伍建设,搭建开放型实验平台与提升实验教学方法,加强实验室产学研联合培养等渠道,加强创新型人才培养.

目的 研究古钩藤的化学成分.方法 采用硅胶、ODS、MCI、制备高效液相等色谱和重结晶等方法进行分离和纯化,对分得的单体化合物根据理化性质和波谱数据鉴定结构.结果 从古钩藤中分离并鉴定了16个化合物,分别为咖啡酸乙酯(1)、豆甾醇(2)、germanicol docosanoate(3)、β-谷甾醇(4)、羽扇豆醇(5)、正十五烷酸(6)、β-胡萝卜苷(7)、3-乙酰氧基齐墩果酸(8)、β-乳香酸(9)、熊果酸(10)、4-羟基香豆素(11)(11)、水杨酸(12)、隐绿原酸(13)、绿原酸(14)、新绿原酸(15)、咖啡酸(16)、异绿原酸A(17)、异绿原酸C(18).结论 化合物1-18均为首次从该植物中分离得到.

[目的]对萝藦科眼树莲属植物眼树莲进行化学成分研究.[方法]将眼树莲药材用75％乙醇浸提,通过反复的柱色谱、重结晶等现代中药化学分离手段进行化学组分分离纯化,得到单体化合物,通过NMR、MS等波谱技术并结合文献数据鉴定化合物结构.[结果]从眼树莲的乙醇提取物分离纯化得到6个化合物,分别鉴定为木栓酮(1)、β-谷甾醇(2)、丁香酸(3)、异香草酸(4)、眼树莲二醇(5)、羽扇豆醇(6).[结论]化合物1～5首次从该植物中分离得到,为后续研究眼树莲化学成分及药理作用的物质基础提供了理论依据.

文章从研究生培养质量、创新能力培养出发,以广西中药大学中药学研究生培养为例,探讨建立可持续发展的导师团队,对构建"开放""交叉""贯通"的创新型人才培养体系的意义.

科研实践平台是培养研究生科研创新能力的关键切入点.通过综合运用科研创新、创新竞赛、教学实践、实践教学、学术交流等平台培养研究生创新思维,开拓学术视野、提高动手操作能力,在实验和实际操作中获得创新想法,最终提升研究生的创新能力;完善实验资源共享管理制度,为研究生科研能力的培养提供保障.

目的 建立HPLC法同时测定九龙盘Antenoronfiliforme (Thunb) Rob.et Vaut.中没食子酸、原儿茶酸、异槲皮苷、鞣花酸、槲皮素、木犀草素的含有量.方法 九龙盘80％甲醇提取物采用COSMOSIL 5C18-PAQ色谱柱(250 mm×4.6 mm,5μm);流动相甲醇-0.2％磷酸水溶液,梯度洗脱;体积流量0.8 mL/min;双波长切换检测;柱温35℃.结果 6种成分在各自范围内线性关系良好(r＞0.999 0),平均加样回收率为85.95％ ～ 103.40％,RSD为0.5％ ～3.0％.结论 该方法准确稳定,重复性好,可用于九龙盘的质量控制.

[Objectives] This paper aimed to study the quality standard of pummelo pericarp from Guangxi. [Methods] The medicinal material was identified by microscopy and thin-layer chromatography, and the contents of moisture, total ash, acid-insoluble ash and extract in the medicinal material were determined according to the methods of the Chinese Pharmacopoeia（2010 edition）. [Results] The content limits for moisture, total ash, acid-insoluble ash and extract were determined（moisture ≤ 12%; total ash ≤ 6.0%; acid-insoluble ash ≤ 0.6%; water-soluble extract ≥ 43.0%; naringin ≥ 10.00 mg/g）. The micro-characteristics of pummelo pericarp were identified, and a thin-layer chromatography identification and content determination method was established. [Conclusions] The established method can provide an experimental basis for the formulation of quality standard for pummelo pericarp.

目的:探讨藤茶双氢杨梅树皮素(APS)联合环磷酰胺(CTX)抗肿瘤的作用.方法:将接种H22的小鼠按瘤体积大小随机分为6组:模型对照组、环磷酰胺组、APS高剂量组、APS低剂量+环磷酰胺组、APS中剂量+环磷酰胺组、APS高剂量+环磷酰胺组,每组14只.环磷酰胺组予环磷酰胺溶液灌胃,剂量为0.02 g/kg;APS高剂量组予双氢杨梅树皮素灌胃,剂量为0.60 g/kg;APS低剂量+环磷酰胺组:灌胃双氢杨梅树皮素0.15 g/kg+腹腔注射环磷酰胺0.02 g/kg;APS中剂量+环磷酰胺组:灌胃双氢杨梅树皮素0.30 g/kg+腹腔注射环磷酰胺0.02 g/kg;APS高剂量+环磷酰胺组:灌胃双氢杨梅树皮素0.60 g/kg+腹腔注射环磷酰胺0.02 g/kg;各组灌胃容积均为10 ml/kg.给药10 d后,计算小鼠的抑瘤率、脾指数、胸腺指数、肝脏指数,分析APS联合环磷酰胺抗肿瘤的作用.结果:APS可抑制H22荷瘤小鼠肿瘤的生长,抑瘤率为20.72％;单用CTX组抑瘤率为33.5％,APS低、中、高剂量与CTX联用后抑瘤率分别为47.07％、48.52％和60.26％;与单用CTX组比较,联合用药明显提高了对H22荷瘤小鼠的抑瘤作用(P<0.05).与模型对照组比较,CTX组脾指数和胸腺指数明显下降(P<0.01);与CTX组比较,APS中、高剂量与CTX联合用药后可提高小鼠的脾指数和胸腺指数,差异有统计学意义(P<0.05或P<0.01);APS低剂量与CTX联合用药后可明显提高小鼠的胸腺指数(P<0.01);APS高剂量与CTX联合用药后还可明显提高小鼠的肝脏指数(P<0.01).结论:APS对H22肿瘤具有抑制作用,与环磷酰胺联用能增强环磷酰胺对H22荷瘤小鼠的抗肿瘤作用.

白叶藤属植物在我国分布广泛,药用价值前景良好,其化学成分主要为生物碱类、苯丙素类、挥发油等化合物,具有镇痛、抗炎、降血糖、降血脂、抗菌、抗疟、保肝和抗氧化等药理作用,同时又可引起神经毒性、生殖毒性和细胞毒性等毒副作用.本文对白叶藤属植物的化学成分、药理作用和毒理研究进行综述,以期为该属植物的深入研究和进一步开发利用提供科学依据.

目的:优化参芪益母膏制备工艺,确定有效成分提取的最佳工艺.方法:利用不同浓度的乙醇进行提取,考察制剂中总黄酮、总皂苷及浸膏得率的提取工艺;采用L9(34)正交进行设计,考察用水提取制剂中盐酸益母革碱及浸膏得率的工艺.结果:醇提部分最佳条件是:加8倍量70％乙醇,回流提取3次,每次1.5h;水提部分最佳条件是:加12倍量水浸泡60 min,煎煮3次,每次2h;考察活性成分含量较高,浸膏得率高.结论:该提取工艺简单,能保证参芪益母膏中有效成分的含量,质量稳定可控.

目的:研究赤苍藤的化学成分.方法:采用硅胶柱色谱分离赤苍藤药材乙酸乙酯部位的油状物,对其进行甲酯化反应,采用气相色谱-质谱联用分析技术分析其化学成分.结果:从组分1中鉴定出8个化学成分,主要含十七烷酸甲酯(22.29％)、油酸甲酯(18.29％);从组分2中鉴定出5个化学成分,主要含有11,14-十八碳二烯酸甲酯(28.95％)、硬脂酸甲酯(21.71％).结论:赤苍藤乙酸乙酯部位的主要成分为脂肪酸、烯酸类化合物.

为完善和提高参芪益母膏的质量标准,增加盐酸益母草碱、黄芪甲苷含量指标成分,建立了测定盐酸益母草碱及黄芪甲苷含量的方法.结果表明测定盐酸益母草碱、黄芪甲苷分別在7.00～42.00 mg·L-1(r=0.9999)和0.646～3.230μg(r=0.9997)时,与峰面积的线性关系良好,平均回收率分别为99.44％(RSD值为0.41％)和99.04％(RSD值为0.95％).本文建立的参芪益母膏的HPLC及HPLC-ELSD法具有高精度和良好的重复性,可用于参芪益母膏的质量控制.

目的:研究全国29个产地对叶百部总生物碱的HPLC图谱与止咳作用的相关性,建立各产地对叶百部总生物碱与止咳作用的谱效关系,阐明止咳作用的物质基础.方法:采用灰色关联度分析方法,对全国29个产地的对叶百部总生物碱的HPLC图谱的8个特征峰峰面积与止咳的2个评价指标(2 min内咳嗽次数、抑咳率)进行相关性分析,研究对叶百部总生物碱止咳作用的谱效关系.结果:对叶百部总生物碱对浓氨水致小鼠咳嗽有显著的抑制作用,与百部的止咳功效一致.在全国29个产地的对叶百部总生物碱HPLC图谱中,1号金刚大碱色谱峰峰面积大、关联度强;2～8号色谱峰对止咳作用也有较大的贡献,关联度差距较小,对止咳作用起协同作用.结论:全国29个产地的对叶百部总生物碱HPLC图谱与止咳作用具有相关性.通过谱效关系研究,可阐明对叶百部止咳作用的物质基础,为其质量控制与评价提供一定的理论依据.

目的:比较超临界二氧化碳萃取法提取石岩枫茎与叶挥发油成分.方法:使用超临界二氧化碳萃取法(SFE-CO2)分别提取石岩枫茎与叶的挥发油,并通过气相色谱-质谱联用(GC-MS)分析挥发油成分.结果:石岩枫茎分离出20个化合物,鉴定出15个化合物,占总流出组分的77.37％,石岩枫叶分离出35个化合物,鉴定出27个化合物,占总流出组分的79.68％,叶比茎鉴定出的化合物的种类多出12种,共有成分为9个.结论:两种部位分离的挥发油成分存在明显差异.

目的 采用酸性染料比色法测定百部流浸膏中总生物碱的含量,为百部流浸膏质量标准的提升提供依据.方法 取0.1 mL供试品溶液,置具塞离心管中,加入5 mL溴甲酚绿、7.0 mL pH4.6的邻苯二甲酸氢钾缓冲液、6 mL二氯甲烷溶液,密塞,涡旋2 min,静置30 min,取二氯甲烷层溶液置10 mL量瓶中,用二氯甲烷定容,在425 nrn处测定吸光度.结果 对叶百部碱的标准曲线为:Y=949.76X-10.756(r2 =0.9975),线性范围为53.125 ～ 850 μg· mL-1;平均回收率为105.20％,RSD =4.16％.13份百部流浸膏样品的总生物碱含量为14.01 ～74.56 mg·mL-1,建议百部流浸膏中总生物碱的含量不低于11.21 mg·mL-1.结论 酸性染料比色法具有良好的专属性、精密度和稳定性,操作简便,可作为百部流浸膏生物碱含量测定的有效方法.

[Objectives] To establish a method for determining the content of caffeic acid in Zhuang Medicine Cryptolepis buchananii. [Methods] The content of caffeic acid in C. buchananii leaves was determined by high performance liquid chromatography（HPLC）. [Results] The results of HPLC determination showed that the RSD values were less than 3%, and the content of caffeic acid in 10 batches of C. buchananii leaves was in the range of 0.096 5%-0.477 2%. [Conclusions] This method is accurate and reliable, has good linear relationship and high precision, and can be used for quality evaluation of C. buchananii.