Binary classification in imbalanced tabular datasets remains a significant challenge in machine learning, as conventional risk-stratification models exhibit limited discriminative performance and fail to capture nonlinear interactions among heterogeneous features. Existing approaches often suffer from three critical limitations: model-selection uncertainty across heterogeneous data distributions, systematic bias toward majority classes when training on imbalanced datasets, and insufficient interpretability for high-stakes decision-making applications. This work proposes a heterogeneous stacking ensemble framework that integrates five complementary base learners—Random Forest, XGBoost, LightGBM, CatBoost, and a Multi-Layer Perceptron—through an L2-regularized logistic regression meta-learner trained on out-of-fold predictions. To address class imbalance, we incorporate an adaptive Borderline-SMOTE oversampling strategy within a leakage-free cross-validation pipeline that concentrates synthetic sample generation in high-difficulty borderline regions. The framework is developed on a multi-institutional dataset of 4,127 instances with 28 features and externally validated on two independent cohorts (n=612, n=489). The proposed approach achieves an AUC of 0.892 (95

Background:Mounting evidence points to a potential link between statin therapy and erectile dysfunction (ED). However, the underlying mechanisms remain elusive, particularly concerning potential statin disruption of the inflammatory and immunofibrotic microenvironment within erectile tissue. This study sought to elucidate statin-associated ED and identify key molecular and cellular mediators driving this process. Methods:An integrative strategy merging real-world pharmacovigilance, network pharmacology, and toxicological analyses was deployed to explore drug-associated ED. Drug-related and disease-associated targets were intersected and scrutinized using protein-protein interaction (PPI) networks to pinpoint key regulatory genes. Gene expression patterns were evaluated through bioinformatics analyses and validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Molecular docking and molecular dynamics (MD) simulations assessed drug-target interaction stability. Furthermore, single-cell RNA sequencing (scRNA-seq) analysis characterized cell-type-specific alterations and intercellular communication dynamics within erectile tissue. Results:Disproportionality analysis identified atorvastatin (ROR = 3.36, 95% CI = 3.04-3.70) and rosuvastatin (ROR = 3.22, 95% CI = 2.81-3.69) as statins significantly linked to ED, exhibiting moderate predicted toxicity. Four key genes-FGFR1, SERPINE1, TGFB2, and TGFBR2-emerged as potential mediators connecting statin exposure to ED. FGFR1 expression plunged significantly, while SERPINE1 expression surged markedly in ED samples, findings consistently observed in both transcriptomic analyses and RT-qPCR validation. Molecular docking and MD simulations demonstrated stable binding between atorvastatin and FGFR1. Notably, scRNA-seq analysis revealed fibroblasts as central immunomodulatory cells in ED, with their intercellular communication dysregulation closely related to the abnormal activation of the FGFR1 axis, which further mediates the local immunofibrotic disorder of the corpus cavernosum. Conclusion:This study delivers convergent evidence that atorvastatin and rosuvastatin associate with ED and implicates dysregulated inflammatory and immunofibrotic remodeling of erectile tissue as a potential underlying mechanism. The identified key genes and fibroblast-centered cellular interactions yield fresh insights into statin-associated ED and highlight promising molecular targets for future translational and immunomodulatory therapeutic strategies.

The optimal specimen extraction site after retroperitoneal laparoscopic donor nephrectomy is debated. This study compared abdominal versus lumbar incisions on hernia risk, motor function, and quality of life. This prospective, single-blind RCT, 240 living kidney donors were randomized 1:1 to an abdominal diagonal or a lumbar horizontal extraction incision. Primary outcomes were incisional hernia incidence at 12 months (verified by ultrasound) and abdominal wall motor function deficits at 6 months (measured by dynamometry and surface electromyography). Secondary outcomes included sensory disturbances, pain, analgesic requirements, and SF-36 scores. Of 240 randomized donors, 234 completed 12-month follow-up (117 abdominal, 117 lumbar group). Incisional hernia occurred in 3 patients (2.6

Renal ischemia-reperfusion (I/R) significantly contributes to acute kidney injury (AKI), causing substantial oxidative stress and metabolic disruptions. Ferroptosis, a Fe2+-dependent form of regulated cell death characterized by lipid peroxide accumulation, is the predominant cause of renal I/R injury (RIRI). Here, carbon dot (C-dot) nanozymes that inhibit ferroptosis by regulating Fe2⁺ levels and scavenging reactive oxygen species, offering a potential treatment for RIRI are reported. C-dots chelate Fe2⁺ via surface carbonyl, hydroxyl, and carboxyl groups to reduce free Fe2⁺ levels, suppress the Fenton reaction, and limit hydroxyl radical generation. Additionally, C-dots scavenge superoxide anions and hydroxyl radicals to restore redox balance. By targeting the kidneys, C-dots effectively reduce renal iron overload and lipid peroxidation to prevent ferroptotic cell death in the renal I/R male mice model. RNA sequencing (RNA-seq) analysis further confirms the crucial roles of C-dots in mitigating oxidative stress, preserving iron homeostasis, and downregulating acyl-CoA synthetase long-chain family member 4 (ACSL4) after I/R. This work emphasizes the perfect alignment between the multifunctional roles of C-dots and the conditions required for inhibiting ferroptosis and offers an innovative strategy to treat RIRI effectively.

Clear cell renal cell carcinoma (ccRCC) is known as the most common type of renal cancer. Recently, a series of advances have been made in targeted therapy for ccRCC. To combat this highly metastatic tumor, novel therapeutic targets still need to be developed. C-type lectins (CLECs) contain a characteristic C-type lectin-like domain and affect several physiological functions. The effects of C-type lectin 2D (CLEC2D) on cancer progression have been revealed in several types of cancers; however, its expression in ccRCC tissues, and the possible effects on the progression and metastasis of ccRCC, are still unclear. Herein, we found the high mRNA and protein levels of CLEC2D in ccRCC tissues. We further found that CLEC2D expression was correlated with the prognosis of ccRCC patients and correlated with the tumor size (p = 0.019*) of patients. In addition, CLEC2D affected tumor immune infiltration, confirmed by the further analysis. CLEC2D knockdown suppressed the proliferation of ccRCC cells in vitro and restrained ccRCC tumor growth and immune infiltration in mice. Therefore, we believe that CLEC2D has the potential to serve as a promising ccRCC therapeutic target.

目的 探讨半导体蓝激光手术系统在日间手术模式下治疗非肌层浸润性膀胱肿瘤的可行性及安全性.方法 回顾性分析西安交通大学第一附属医院2022年6月—2022年9月22例经过门诊筛查并自愿接受蓝激光日间手术的膀胱肿瘤患者的临床资料,平均年龄55.8岁,肿瘤大小平均1.4 cm.患者入院当天即安排手术,采用蓝激光整块剜除技术切除膀胱肿瘤,术后当天或者第2天晨即停止膀胱冲洗并拔除导尿管后出院.记录患者的基线资料、院前等待时间、手术时间、住院时间、血红蛋白下降量、并发症发生及处理情况、随访、医疗费用、患者满意率.结果 等待住院时间2～7 d,平均(4.1±1.3)d;手术时间29～50 min,平均(40.8±5.5)min;住院时间0.6～1.2d,平均(0.9±0.2)d;血红蛋白下降量(g/L)1～8g/L,平均(3.8±1.8)g/L;留置尿管时间0.5～1d,平均(0.7±0.1)d;人均费用最少13790～16811元,平均(14941.5±690.2)元;满意度为100.0％.术中、术后发生并发症2例,均为轻度不良事件,其中1例患者发生膀胱炎,给予口服头孢克肟2d后症状消失;1例出现膀胱痉挛,给予口服琥珀酸索利那新片后缓解;术中未发生闭孔神经反射或膀胱穿孔等不良事件;拔除尿管后,未发生尿潴留事件.结论 本研究首次尝试了蓝激光日间手术用于膀胱肿瘤的治疗,证实了在合适的患者选择及成熟的技术条件下,蓝激光日间手术是一种治疗膀胱肿瘤安全、可行、经济、高效的模式,可以在有条件的医院推广.

Following the publication of the above paper, it was drawn to the Editor's attention by a concerned reader that certain of the tumour images in Fig. 3A and the immunohistochemistry data in Fig. 3C on p. 7, and colony formation assay data shown in Fig. 4F on p. 8 were strikingly similar to data that had already appeared in previous publications. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were under consideration for publication, prior to its submission to International Journal of Molecular Medicine, the Editor has decided that this paper should be retracted from the Journal. After having been in contact with the authors, they accepted the decision to retract this paper. The Editor apologizes to the readership for any inconvenience caused. [International Journal of Molecular Medicine 47: 99, 2021; DOI: 10.3892/ijmm.2021.4932].

目的 对比研究蓝激光整块剜除术与传统等离子电切术在非肌层浸润性膀胱肿瘤(NMIBC)治疗中围手术期的有效性和安全性指标的变化.方法 选取2018年10月—2019年12月西安交通大学第一附属医院确诊的膀胱肿瘤患者50例,采用随机、不完整设盲、平行对照设计和非劣效检验方法,对照组(电切组)使用等离子电切环行传统的经尿道膀胱肿瘤电切术,试验组(蓝激光组)使用创新半导体蓝激光手术系统行经尿道膀胱肿瘤整块剜除术,对比两组在膀胱肿瘤有效切除率、手术时间、术后留置尿管时间、住院时间、围手术期血色素变化和闭孔神经反射等指标的差异.结果 蓝激光组纳入24例患者,电切组纳入26例患者,两组术中对所见膀胱肿瘤的切割效能、止血效能均达到100％;蓝激光手术时间要稍长于电切组(55 min vs.42 min,P=0.009),但血色素下降较电切组小(5.7g/L vs.10.4g/L,P=0.007).两组尿管留置时间、住院时间、再次手术率差异无统计学意义;电切组有3例患者发生闭孔神经反射,而蓝激光组未有发生.结论 与传统电切相比,蓝激光对膀胱肿瘤组织具有良好的汽化切割和凝固止血效能,直出模式下能整块剜除肿瘤且出血少、无闭孔神经反射发生,可作为NMIBC手术治疗的一种崭新、高效、安全、易于掌握的新方法,但其对膀胱肿瘤术后复发率、进展率等指标的影响还有待进一步研究.

Objectives:Hexokinase 2 (HK2) is one of the key factors involved in the development of several human cancers. However, its role in immune cell infiltration (ICI) and tumor development in renal cell carcinoma is not yet known. Thus, we aimed to explore its relationship with ICI, overall survival, and prognosis of renal cell carcinoma.Methods:In this study, RNA-seq data from renal cancer and normal tissues were extracted from TCGA and the relationship between HK2 expression and pathological features of RCC patients was analyzed using the GEPIA and UALCAN databases. Subsequently, Western blot and qRT-PCR were performed to analyze the protein and mRNA expression of HK2 in renal cell carcinoma tissues and cell lines. Lastly, various bioinformatics tools were applied to determine the immune cell infiltration, survival, and developing prediction model.Results:The analysis of RNA-seq data revealed a high expression of HK2 in renal cell carcinoma; furthermore, Western blot and qRT-PCR also showed high expression of HK2 in renal cancer tissues and cell lines. The high expression of HK2 showed a significant positive correlation with the advanced stage of the tumor, lymph node metastasis, and worst survival in renal carcinoma patients. The high expression of HK2 was further identified as an independent risk factor of RCC patients; it also showed a significant positive immune cell infiltration RCC tumor microenvironment including macrophages, B cells, neutrophils, dendritic cells, and CD8+ T cells.Conclusion:the expression of HK2 is positively correlated with the immune cell infiltration and prognosis of renal cell carcinoma patients, thus playing an important role in renal cancer development.

目的 分析肾透明细胞癌中KIF4A的表达及其对患者术后生存的预测价值.方法 选取接受根治性肾切除术的肾透明细胞癌患者70例,术中获取癌组织和癌旁组织(距离肿瘤3 cm),检测KIF4A的表达.查看患者病案信息,统计患者的性别、年龄、临床分期、Fuhrman核分级、肿瘤大小、淋巴结转移和远处转移情况.随访60个月,统计患者生存时间.分析癌组织和癌旁组织KIF4A阳性表达情况,KIF4A表达与临床病理特征的关系,KIF4A表达对预后的影响.结果 肾透明细胞癌组织KIF4A阳性率高于癌旁组织(P<0.05).KIF4A表达与年龄、临床分期、Fuhrman核分级、肿瘤大小、淋巴结转移和远处转移有关(P<0.05),与性别无关(P>0.05).KIF4A阴性表达患者平均生存时间为(47.69±6.02)个月,阳性表达患者平均生存时间为(30.59±4.05)个月,差异有统计学意义(P<0.05).KIF4A阳性表达与生存时间呈负相关.结论 肾透明细胞癌中KIF4 A阳性表达影响术后生存时间,可作为预测术后预后的指标.

Objective:To explore the relationship between epithelial-mesenchymal transition (EMT) and ureteral stricture after holmium laser lithotripsy for ureteral calculi.Methods:A total of 34 patients with ureteral stricture after holmium laser lithotripsy for ureteral calculi in The First Affiliated Hospital of Henan University of Science and Technology between June 2019 and June 2021 were selected as the stricture group, including 19 males and 15 females, with an age of (44.54±10.35) years old. Another 34 patients without ureteral stricture after ureteral holmium laser lithotripsy during the same period were regarded as the non-stricture group according to the ratio of 1:1, including 20 males and 14 females, with an age of (45.56±9.57) years old. χ2 test or independent sample t test was used to compare the general data and serum levels of high mobility group protein B1 (HMGB1), interleukin-1β (IL-1β), and transforming growth factor-β1 (TGF-β1) between the two groups, and the receiver operating characteristic curve (ROC) was used to analyze the predictive efficacies of serum HMGB1, IL-1β, and TGF-β1 levels in ureteral stricture after holmium laser lithotripsy. Results:Before surgery, there were no statistically significant differences in the serum HMGB1, IL-1β, and TGF-β1 levels between the two groups (all P>0.05); on the postoperative 7th day, the serum levels of HMGB1, IL-1β, and TGF-β1 in the stenosis group were (15.98±1.11) μg/L, (24.23±1.54) μg/L, and (381.58±38.52) ng/L, which were higher than those in the non-stenosis group [(14.21±1.02) μg/L, (23.11±1.08) μg/L, and (318.23±27.54) ng/L], with statistically significant differences (all P<0.05). The ROC analysis showed that serum levels of HMGB1, IL-1β, and TGF-β1 on the postoperative 7th day could be used as predictors for ureteral stenosis after holmium laser lithotripsy ( Z=7.421, 2.759, and 10.740; all P<0.05), the area under the curve (AUC) was 0.848, 0.679, and 0.898, respectively, and serum TGF-β1 had the highest predictive efficiency. Conclusions:Ureteral injury caused by holmium laser lithotripsy can lead to the increases of serum HMGB1, IL-1β, and TGF-β1 levels. Patients' serum HMGB1, IL-1β, and TGF-β1 levels can be used as predictors for ureteral stricture after holmium laser lithotripsy.

Objective. Currently, lots of scholars have proved that the expression of NCAPG is associated with the prognosis of several cancers, while the relationship between NCAPG and renal clear cell carcinoma remains unclear, so the main aim of this research is to explore the effects of NCAPG on the progression of renal clear cell carcinoma. Methods. We observed the differential expression of NCAPG in several cancers from GEPIA online database, and the expression of NCAPG in renal clear cell carcinoma and normal tissue was compared and further verified by IHC assay. CCK-8 assay and clone formation experiment were conducted to observe the change of NCAPG on the proliferation. GraphPad was used for data analysis, and t-test and χ2 analysis were used to analyze the correlation between NCAPG/CDK1 and renal clear cell carcinoma. Results. NCAPG was upregulated in renal clear cell carcinoma compared with the normal tissue, and the expression of NCAPG was associated with the clinical prognosis of pancreatic cancer especially with tumor size (P=0.010). Knockdown NCAPG could restrain the proliferation of renal clear cell carcinoma. CDK1 was found to be tightly related with NCAPG, and the expression of CDK1 was also associated with the prognosis. Conclusions. NCAPG was upregulated in renal clear cell carcinoma, which was related with tumor size and overall survival. NCAPG might promote the proliferation of renal clear cell carcinoma via mediating CDK1. NCAPG/CDK1 complex might provide a new treatment strategy for lots of patients with renal clear cell carcinoma.

Background Prostate cancer (PCa) is one of the most common malignant cancer in males worldwide. Circular RNAs (CircRNAs) are novel type of non-coding RNAs. Recently, circRNAs have been reported participating in various cancers, including prostate cancer. However, the function and mechanism of circ_0057553 remain to be elucidated. Methods and Materials The RNA expression levels of circ_0057553, miR-515-5p, YES proto-oncogene 1 (YES1) and glycolytic genes mRNA were detected by qRT-PCR in PCa tissues or cells. Western blotting was performed to analyze YES1 protein level. Cell viability, migration and invasion and cell apoptosis were assessed by cell counting kit-8 (CCK-8) assay, transwell assay and flow cytometry. In addition, the effects of cell glycolysis were evaluated by measuring lactate production, glucose consumption and adenosine triphosphate (ATP) level. Moreover, dual-luciferase reporter assay was used to detect the target sites of circ_0057553 and miR-515-5p, miR-515-5p and YES1. RNA immunoprecipitation (RIP) was conducted to evaluate the target relationship between circ_0057553 and miR-515-5p. Xenograft mouse model was conducted to measure tumor formation in vivo. Results Circ_0057553 was significantly up-regulated in PCa tissues and cells. Knockdown of circ_0057553 inhibited cell viability, migration, invasion and glycolysis and facilitated apoptosis in PCa cells. Furthermore, circ_0057553 bound to miR-515-5p and miR-515-5p directly targeted YES1. Interestingly, miR-515-5p inhibitor partially rescued the function of circ_0057553 knockdown, while YES1 restored the effects of miR-515-5p overexpression. Circ_0057553 down-regulation remarkably decreased tumor volume and weight in vivo. Conclusion Circ_0057553 affected PCa cell viability, migration, invasion, apoptosis and glycolysis through miR-515-5p/YES1 axis.

Introduction Ribosome binding protein 1 (RRBP1) is reported to be correlated with tumor formation and progression. However, the role of RRBP1 in bladder cancer is unclear. In this study, we aimed to investigate the expression of RRBP1 and its influence on cell proliferation in bladder cancer. Methods Quantification real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were used to detect the expression levels of RRBP1 in 138 bladder cancer and matched adjacent normal bladder tissues. Then, the clinical significance of RRBP1 in bladder cancer was evaluated. The effect of RRBP1 on cell proliferation and its potential mechanism were further explored. Results Results show that the mRNA levels of RRBP1 in bladder cancer were significantly higher compared with those in normal tissues (P< 0.001). IHC results show the high-expression rate of RRBP1 in bladder cancer was 68.8%, which was significantly greater than those in normal tissues (40.6%, P< 0.001). RRBP1 high-expression was significantly associated with differentiation, T stage and lymph node metastasis in bladder cancer (P< 0.05). The overall survival time of patients with RRBP1 high-expression was significantly reduced compared to those with RRBP1 low-expression. Moreover, RRBP1 overexpression significantly promoted cell proliferation, which was correlated with Smad1/Smad3/TGF-β1 signal pathway. Conclusion RRBP1 high-expression correlates with prognosis and promotes cell proliferation in bladder cancer, which could be a potential biomarker.

目的 评估经后腹腔镜单解剖层面巨大肾上腺肿瘤切除术的手术技巧及临床效果.方法 回顾性分析笔者医院2015年1月 ～2019年12月行后腹腔镜巨大肾上腺肿瘤切除术60例患者的临床资料,单解剖层面肾上腺肿瘤切除术32例,非单解剖层面肾上腺肿瘤切除术28例.比较两组患者的手术指标及围术期的相关临床参数.结果 60例巨大肾上腺肿瘤均在后腹腔镜下完成,两组肿瘤患者的基本情况、肿瘤位置、病理类型等比较,差异均无统计学意义(P>0.05).经后腹腔镜单解剖层面肾上腺肿瘤切除术在手术时间、腹膜破损率、术中出血量及术后引流量等方面均优于非单解剖层面肾上腺肿瘤切除术,差异均有统计学意义(P<0.05).而在肿瘤完整切除率及术后恢复方面,两组比较差异无统计学意义(P>0.05).结论 经后腹腔镜单解剖层面巨大肾上腺肿瘤切除术疗效显著,值得在临床上应用推广.

目的:探讨加速康复外科(ERAS)理念对经尿道选择性绿激光前列腺汽化术(PVP)患者术后康复的安全性和有效性.方法:回顾2018年6月至2019年10月在河南科技大学第一附属医院行经尿道选择性绿激光PVP治疗的61例前列腺增生患者,其中采用加速康复理念进行围手术期管理30例(ERAS组),按照传统围手术期管理31例(对照组).比较两组手术时间、术后6h视觉模拟评分(VAS)、术后第ld血白细胞计数、术后首次排气时间、国际前列腺症状评分(IPSS)、生活质量(QOL)评分、最大尿流率(Qmax)、术后尿管留置时间、住院时间以及出院3个月内并发症发生情况等.结果:两组术后6小时VAS评分、术后排气时间、留置尿管时间、平均住院时间比较差异有统计学意义(P＜0.05).两组术后3个月IPSS评分、术后3个月QOL评分、术后3个月Qmax比较差异均无统计学意义(P＞0.05).两组术后并发症发生率比较差异无统计学意义(P＞0.05).结论:ERAS应用于经尿道选择性绿激光PVP围手术期的管理满足安全性、有效性的要求,有助于缓解术后早期疼痛感,缩短肠道恢复、住院的时间,使患者能够更快地出院和康复.

目的:探讨肾肿瘤合并肾血管变异的临床特点,以及后腹腔镜根治性肾切除术中肾血管变异的处理方法与注意事项.方法:回顾性分析2013年1月～2016年6月我院肾肿瘤患者的临床资料,统计并分析血管变异种类及特点,后腹腔镜手术中观察血管异常情况,并与具有正常血管的肾肿瘤患者手术时间、出血量和住院时间进行对比.结果:283例肾肿瘤患者中71例合并血管变异,肾血管变异患者(变异组)手术中转开放4例,肾血管正常患者(正常组)中转开放手术2例.变异组平均手术时间141 min、正常组120.6 min;变异组平均出血量178.9ml,正常组118.6 ml;两组手术时间及术中出血量比较差异有统计学意义(P＜0.05).变异组术后住院为8.2d,正常组为7.9d,两组比较差异无统计学意义(P＞0.05).结论:肾肿瘤患者血管变异率较高,血管变异增加了手术复杂性,术前明确动脉血供特点,术中仔细操作,是手术成功的关键,在夹闭肾静脉前先行阻断试验可以判断肾脏是否仍存在动脉血供,防止血管漏扎.

Objective To evaluate the clinical efficacy and safety of needleless single incision in the treatment of female stress urinary incontinence (SUI) .Methods The clinical data of 37 patients with pressure incontinence who received surgical treatment from January 2015 to January 2017 were selected, including 27 cases of pure pressure incontinence and 10 cases of mixed type. The average age was 53.1 years. The medical history was 1-20 years, with an average of 6.1 years. The follow-up time was 7-24 months, with an average of 15.3 months. All patients completed iciq-sf, iiq-7, and sex quality questionnaire before and after surgery (pisq-12) before and after surgery. The wilcoxon rank sum test was used to compare the preoperative scores. Results Thirty-seven cases completed the operation under local anethesia, 30 patients (81.1％) were cured 3 months after surgery, 6 cases (16.2％) were improved, 1 case was failed (2.7％); 29 cases (78.4％) were cured 12 months after surgery (78.4％), 7 cases were improved (19.4％), and the total effective rate was 97.3％ (2.7％), 1 case was failed. The score of IIQ-7 and iciq-sf at 3 and 12 months after operation were significantly lower than those before operation (P＜0.05) .The postoperative score of pisq-12 was increased, there was significant difference compared with the preoperative score (P＜0.05) .Conclusions Single incision needleless sling operation is simple, more minimally invasive and has fewer complications, which is an effective method for the treatment of female stress incontinence.

INTRODUCTION:Recently, increasing evidence has shown that long non-coding RNAs (lncRNAs) play critical roles in tumor progression and development. However, the expression pattern and biological function of lncRNA HULC (highly upregulated in liver cancer) in prostate cancer (PCa) remain largely unclear.MATERIAL AND METHODS:The expression of lncRNA HULC in 53 paired PCa tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The χ2 test was used to explore the association of lncRNA HULC expression with clinicopathologic features. Kaplan-Meier analysis was used to detect the association between HULC expression and overall survival of PCa patients. Furthermore, the function of HULC in cell growth and metastasis was detected in PCa cells.RESULTS:Our data showed that HULC expression was upregulated in PCa tissues and cell lines compared to adjacent non-tumor tissues and the normal prostate cell line RWPE-1 (p < 0.05). High HULC expression was positively associated with advanced clinicopathologic features and poor overall survival (OS) for PCa patients (p < 0.05). HULC inhibition suppressed PCa cell growth and metastasis both in vitro and in vivo (p < 0.05). Furthermore, HULC knockdown reduced N-cadherin and vimentin expression and increased E-cadherin expression in PCa cells (p < 0.05).CONCLUSIONS:Our data suggested that lncRNA HULC might play oncogenic roles in PCa progression, which provided a novel therapeutic strategy for PCa patients.