The optimal specimen extraction site after retroperitoneal laparoscopic donor nephrectomy is debated. This study compared abdominal versus lumbar incisions on hernia risk, motor function, and quality of life. This prospective, single-blind RCT, 240 living kidney donors were randomized 1:1 to an abdominal diagonal or a lumbar horizontal extraction incision. Primary outcomes were incisional hernia incidence at 12 months (verified by ultrasound) and abdominal wall motor function deficits at 6 months (measured by dynamometry and surface electromyography). Secondary outcomes included sensory disturbances, pain, analgesic requirements, and SF-36 scores. Of 240 randomized donors, 234 completed 12-month follow-up (117 abdominal, 117 lumbar group). Incisional hernia occurred in 3 patients (2.6

Clear cell renal cell carcinoma (ccRCC) is known as the most common type of renal cancer. Recently, a series of advances have been made in targeted therapy for ccRCC. To combat this highly metastatic tumor, novel therapeutic targets still need to be developed. C-type lectins (CLECs) contain a characteristic C-type lectin-like domain and affect several physiological functions. The effects of C-type lectin 2D (CLEC2D) on cancer progression have been revealed in several types of cancers; however, its expression in ccRCC tissues, and the possible effects on the progression and metastasis of ccRCC, are still unclear. Herein, we found the high mRNA and protein levels of CLEC2D in ccRCC tissues. We further found that CLEC2D expression was correlated with the prognosis of ccRCC patients and correlated with the tumor size (p = 0.019*) of patients. In addition, CLEC2D affected tumor immune infiltration, confirmed by the further analysis. CLEC2D knockdown suppressed the proliferation of ccRCC cells in vitro and restrained ccRCC tumor growth and immune infiltration in mice. Therefore, we believe that CLEC2D has the potential to serve as a promising ccRCC therapeutic target.

Following the publication of the above paper, it was drawn to the Editor's attention by a concerned reader that certain of the tumour images in Fig. 3A and the immunohistochemistry data in Fig. 3C on p. 7, and colony formation assay data shown in Fig. 4F on p. 8 were strikingly similar to data that had already appeared in previous publications. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were under consideration for publication, prior to its submission to International Journal of Molecular Medicine, the Editor has decided that this paper should be retracted from the Journal. After having been in contact with the authors, they accepted the decision to retract this paper. The Editor apologizes to the readership for any inconvenience caused. [International Journal of Molecular Medicine 47: 99, 2021; DOI: 10.3892/ijmm.2021.4932].

目的 分析神经内镜第三脑室底造瘘术(ETV)联合脉络丛烧灼术(CPC)治疗儿童脑积水的临床治疗效果.方法 回顾性分析2016年1月—2019年1月广东三九脑科医院神经外科收治的50例脑积水患儿的临床资料,依治疗方式不同分为观察组(n=26,ETV联合CPC)和对照组(n=24,ETV).分析两组患儿治疗效果情况.结果 观察组患儿在总有效率为96.1％,对照组患儿在总有效率为70.8％,两组比较有统计学意义(P＜0.05).其中观察组中因梗阻性脑积水行联合治疗其有效率为100％,因交通性脑积水性联合治疗其有效率为90％,两者对比无统计学意义(P＞0.05);对照组中因梗阻性脑积水行联合治疗其有效率为84.6％,因交通性脑积水性联合治疗其有效率为27.3％,两者对比无统计学意义(P＞0.05).观察组1例、对照组7例造瘘术后无效,后转为行脑室腹腔分流治疗.观察组出现1例硬膜下积液,1例气颅(2/26,7.7％),对照组1例癫痫、1例感染(2/24,8.3％),两组的并发症比较无统计学意义(P＞0.05);观察组患儿术后半年的平均Gesell发育商数均高于对照组,有统计学意义(P＜0.05).结论 神经内镜下第三脑室底造瘘术联合脉络丛烧灼术治疗儿童脑积水安全有效,显著改善患儿神经发育水平.

目的 探讨经尿道膀胱黏膜电切术联合塞来昔布治疗肠型腺性膀胱炎(CG)的有效性和安全性.方法 选取2019年1月至2021 年6月53例肠型CG患者作为研究对象,按照治疗方式的不同将其分为试验组(n=27,经尿道膀胱黏膜电切术联合塞来昔布治疗)和对照组(n=26,经尿道膀胱黏膜电切术治疗).比较两组的临床症状评分、术后复发情况及临床疗效.结果 术后1、3、6、9、12 个月,试验组的临床症状评分均低于术前(P＜0.05);术后 1、3、6、9、12 个月,试验组的临床症状评分低于对照组(P＜0.05).试验组的术后复发率明显低于对照组(P＜0.05).两组的手术均成功完成,无膀胱穿孔、围手术期大出血、输尿管狭窄等并发症发生.试验组的治疗总有效率明显高于对照组(P＜0.05).结论 经尿道膀胱黏膜电切术联合塞来昔布治疗肠型CG具有良好的临床效果,可降低疾病复发率.

目的 探讨3D打印聚醚醚酮(PEEK)材料在儿童颅骨成形术中的应用效果.方法 回顾性分析2017年1月至2019年6月使用3D打印PEEK材料进行颅骨成形术治疗的31例儿童颅骨缺损的临床资料.结果 所有患儿切口愈合良好,术后7～9d拆线,头型美观,双侧基本对称.术后无感染、死亡、二次手术病例,1例出现硬膜下积液、1例出现皮瓣压伤.术后随访3个月至2年,CT复查显示颅骨双侧均对称良好,无癫痫发作、迟发型排斥反应、迟发型皮下积液、植入物外露、变形、移位等情况.结论 3D打印PEEK材料,和颅骨吻合精度高,对称性好,有良好的生物相容性和生物力学特性,是一种良好的儿童颅骨成形术的材料.

Bladder cancer is the most common malignant tumor of the urinary system, and in China it is first among urogenital system tumors. More therapeutic targets are still urgently required to combat this disease. Lamin B2 (LMNB2) is a type of nuclear lamina filament protein, which is involved in multiple cellular processes, and known as an oncogene affecting the progression of multiple types of cancers. Although the multiple effects of LMNB2 on cancer progression have been elucidated, its possible role in bladder cancer remains unclear. In the present study, it was determined that LMNB2 expression was upregulated in human bladder cancer tissues, and its expression was correlated with the prognosis and the clinical features, including tumor stage (P=0.001) and recurrence (P=0.006) of patients with bladder cancer. In addition, it was further revealed that LMNB2 depletion inhibited bladder cancer cell proliferation, stimulated cell cycle arrest and apoptosis in vitro, and suppressed tumor growth of bladder cancer cells in mice. Furthermore, the present data revealed that LMNB2 promoted the proliferation of bladder cancer cells via transcriptional activation of CDCA3 expression. Therefore, the role of LMNB2 in bladder cancer progression was demonstrated, and may serve as a promising therapeutic target for bladder cancer treatment.

目的 研究经腹膜外腹腔镜前列腺癌根治术(ELRP)用于局限性高危前列腺癌的临床效果.方法 选取2018年10月—2020年12月河南科技大学第一附属医院收治的78例局限性高危前列腺癌患者,将采用经腹腔入路腹腔镜前列腺癌根治术(TLRP)治疗的39作为对照组,将采用经腹膜外腹腔镜前列腺癌根治术(ELRP)治疗的39例作为观察组,对比两组的临床治疗效果.结果 观察组住院时间(13.81±0.46)d、导尿管留置时间(12.31±0.47)d、术后肠功能恢复时间(2.76±0.32)d、术中出血量(131.15±13.24)mL以及手术时间(155.37±22.47)min,与对照组比较均明显降低,对比差异有统计学意义(P＜0.05);观察组拔管时、术后6个月的控尿率(82.05％)明显高于对照组(61.53％),有统计学意义(P＜0.05);但术后1年再作对比,两组控尿率无明显差异,P＞0.05;术后1年,观察组的生化复发率为15.38％,与对照组(12.82％)比较差异无统计学意义(P＞0.05).结论 经腹膜外腹腔镜前列腺癌根治术用于局限性高危前列腺癌治疗疗效良好,且手术时间更短、创伤更小,术后恢复速度更快,值得在临床上推广应用.

Objectives:Hexokinase 2 (HK2) is one of the key factors involved in the development of several human cancers. However, its role in immune cell infiltration (ICI) and tumor development in renal cell carcinoma is not yet known. Thus, we aimed to explore its relationship with ICI, overall survival, and prognosis of renal cell carcinoma.Methods:In this study, RNA-seq data from renal cancer and normal tissues were extracted from TCGA and the relationship between HK2 expression and pathological features of RCC patients was analyzed using the GEPIA and UALCAN databases. Subsequently, Western blot and qRT-PCR were performed to analyze the protein and mRNA expression of HK2 in renal cell carcinoma tissues and cell lines. Lastly, various bioinformatics tools were applied to determine the immune cell infiltration, survival, and developing prediction model.Results:The analysis of RNA-seq data revealed a high expression of HK2 in renal cell carcinoma; furthermore, Western blot and qRT-PCR also showed high expression of HK2 in renal cancer tissues and cell lines. The high expression of HK2 showed a significant positive correlation with the advanced stage of the tumor, lymph node metastasis, and worst survival in renal carcinoma patients. The high expression of HK2 was further identified as an independent risk factor of RCC patients; it also showed a significant positive immune cell infiltration RCC tumor microenvironment including macrophages, B cells, neutrophils, dendritic cells, and CD8+ T cells.Conclusion:the expression of HK2 is positively correlated with the immune cell infiltration and prognosis of renal cell carcinoma patients, thus playing an important role in renal cancer development.

目的 分析肾透明细胞癌中KIF4A的表达及其对患者术后生存的预测价值.方法 选取接受根治性肾切除术的肾透明细胞癌患者70例,术中获取癌组织和癌旁组织(距离肿瘤3 cm),检测KIF4A的表达.查看患者病案信息,统计患者的性别、年龄、临床分期、Fuhrman核分级、肿瘤大小、淋巴结转移和远处转移情况.随访60个月,统计患者生存时间.分析癌组织和癌旁组织KIF4A阳性表达情况,KIF4A表达与临床病理特征的关系,KIF4A表达对预后的影响.结果 肾透明细胞癌组织KIF4A阳性率高于癌旁组织(P<0.05).KIF4A表达与年龄、临床分期、Fuhrman核分级、肿瘤大小、淋巴结转移和远处转移有关(P<0.05),与性别无关(P>0.05).KIF4A阴性表达患者平均生存时间为(47.69±6.02)个月,阳性表达患者平均生存时间为(30.59±4.05)个月,差异有统计学意义(P<0.05).KIF4A阳性表达与生存时间呈负相关.结论 肾透明细胞癌中KIF4 A阳性表达影响术后生存时间,可作为预测术后预后的指标.

目的:研究微创穿刺联合开颅减压治疗高血压脑出血致脑疝患者的疗效及预后.方法:选择2017年6月至2018年2月于广东三九脑科医院就诊的50例高血压脑出血致脑疝患者,按照随机数字法分为对照组和观察组,各25例.对照组行常规开颅血肿清除术及去骨瓣减压术,观察组采取微创穿刺引流联合开颅减压的术式,比较两组患者临床疗效、巴氏指数(BI)评分、格拉斯哥预后量表(GOS)评分及并发症发生率.结果:观察组的总有效率为96.00％,显著高于对照组的72.00％,差异具有统计学意义(P＜0.05);观察组术后3个月BI评分高于对照组,差异具有统计学意义(P＜0.05);观察组GOS评分Ⅳ、Ⅴ级人数占比高于对照组,差异具有统计学意义(P＜0.05).结论:微创穿刺联合开颅减压治疗高血压脑出血致脑疝疗效较好,明显改善患者的预后.

The aim of this study was to investigate the biological function and molecular mechanism of ARPC1A (actin related protein 2/3 complex subunit 1A) in prostate cancer progression. RT–qPCR and IHC results showed that the level of ARPC1A in prostate cancer tissues was significantly higher than that in adjacent tissues. The results of TCGA (the cancer genome atlas) database analysis showed that high expression of ARPC1A indicates poor prognosis in prostate cancer patients. In vitro functional experiments confirmed that downregulation of ARPC1A expression resulted in decreased cell viability and invasive ability of prostate cancer cells, as ARPC1A knockdown promoted ferroptosis. The transcriptional regulation mechanism of STAT3 (signal transduction and activators of transcription 3) on ARPC1A was elucidated by Co-IP, ChIP and luciferase reporter assays. In vivo experiments also supported the in vitro results. We propose that reduced ARPC1A expression inhibits prostate cancer cell viability and invasion in a ferroptotic manner. The ARPC1A level may serve as an independent predictor of prognosis in prostate cancer patients.

目的 对比分析神经导航辅助神经内镜下老年高血压脑出血血肿清除术与微创血肿穿刺外引流的临床效果.方法 选择2017年1月至2018年12月广东三九脑科医院收治的33例高血压脑出血老年患者,根据手术方式不同分为A组和B组,其中A组15例,B组18例.A组采用神经内镜联合导航清除血肿,B组采用导航下钻孔穿刺血肿.比较两组手术时间、术中出血、住院费用、术后监护天数、颅内血肿清除率、预后情况.结果 A组的术后监护天数少于B组,A组的颅内血肿清除率高于B组,A组的总住院费用少于B组,并且A组术后3个月、6个月的Barthel指数明显高于B组,差异有统计学意义(P<0.05);但B组手术时间、术中出血量明显少于A组,差异有统计学意义(P<0.05).结论 神经导航辅助神经内镜治疗老年性高血压脑出血能提高疗效,减少住院时间和改善预后;导航下血肿微创穿刺手术时间短、术中出血少,两者手术效果均安全可行.临床上应具体根据患者实际情况,灵活选择手术方案.

Objective:To explore the relationship between epithelial-mesenchymal transition (EMT) and ureteral stricture after holmium laser lithotripsy for ureteral calculi.Methods:A total of 34 patients with ureteral stricture after holmium laser lithotripsy for ureteral calculi in The First Affiliated Hospital of Henan University of Science and Technology between June 2019 and June 2021 were selected as the stricture group, including 19 males and 15 females, with an age of (44.54±10.35) years old. Another 34 patients without ureteral stricture after ureteral holmium laser lithotripsy during the same period were regarded as the non-stricture group according to the ratio of 1:1, including 20 males and 14 females, with an age of (45.56±9.57) years old. χ2 test or independent sample t test was used to compare the general data and serum levels of high mobility group protein B1 (HMGB1), interleukin-1β (IL-1β), and transforming growth factor-β1 (TGF-β1) between the two groups, and the receiver operating characteristic curve (ROC) was used to analyze the predictive efficacies of serum HMGB1, IL-1β, and TGF-β1 levels in ureteral stricture after holmium laser lithotripsy. Results:Before surgery, there were no statistically significant differences in the serum HMGB1, IL-1β, and TGF-β1 levels between the two groups (all P>0.05); on the postoperative 7th day, the serum levels of HMGB1, IL-1β, and TGF-β1 in the stenosis group were (15.98±1.11) μg/L, (24.23±1.54) μg/L, and (381.58±38.52) ng/L, which were higher than those in the non-stenosis group [(14.21±1.02) μg/L, (23.11±1.08) μg/L, and (318.23±27.54) ng/L], with statistically significant differences (all P<0.05). The ROC analysis showed that serum levels of HMGB1, IL-1β, and TGF-β1 on the postoperative 7th day could be used as predictors for ureteral stenosis after holmium laser lithotripsy ( Z=7.421, 2.759, and 10.740; all P<0.05), the area under the curve (AUC) was 0.848, 0.679, and 0.898, respectively, and serum TGF-β1 had the highest predictive efficiency. Conclusions:Ureteral injury caused by holmium laser lithotripsy can lead to the increases of serum HMGB1, IL-1β, and TGF-β1 levels. Patients' serum HMGB1, IL-1β, and TGF-β1 levels can be used as predictors for ureteral stricture after holmium laser lithotripsy.

目的:探讨肺脏超声对新生儿感染性肺炎的应用价值.方法:回顾性分析我院新生儿重症监护病房(NICU)经临床确诊为感染性肺炎患儿的肺脏超声检查结果及同期有床旁X线检查、CRP及血气分析结果的病例(感染组),将床旁肺脏超声检查结果、肺脏超声评分与X线、CPR及血气分析进行对比及相关性分析;选择同期因非呼吸系统疾病入住NICU患儿(对照组)的肺脏超声作为对照,探讨床旁肺脏超声对新生儿感染性肺炎的应用价值.结果:对照组与感染组之间患儿体重差异无统计学意义;感染组患儿胎龄小于对照组患儿胎龄,差异有统计学意义(P＜0.05);感染组肺脏超声评分与对照组差异有统计学意义(P＜0.05).感染组中,肺脏超声与X线检查诊断感染性肺炎的敏感性之间无统计学意义(P＞0.05).肺脏超声评分与CRP值成中等程度相关,与呼吸指数成正相关(P＜0.05).结论:床旁肺脏超声作为无创、无辐射、高性价比、高诊断率的检查手段,可作为新生儿感染性肺炎协助诊断及随访的常规影像学检查项目.

Objective. Currently, lots of scholars have proved that the expression of NCAPG is associated with the prognosis of several cancers, while the relationship between NCAPG and renal clear cell carcinoma remains unclear, so the main aim of this research is to explore the effects of NCAPG on the progression of renal clear cell carcinoma. Methods. We observed the differential expression of NCAPG in several cancers from GEPIA online database, and the expression of NCAPG in renal clear cell carcinoma and normal tissue was compared and further verified by IHC assay. CCK-8 assay and clone formation experiment were conducted to observe the change of NCAPG on the proliferation. GraphPad was used for data analysis, and t-test and χ2 analysis were used to analyze the correlation between NCAPG/CDK1 and renal clear cell carcinoma. Results. NCAPG was upregulated in renal clear cell carcinoma compared with the normal tissue, and the expression of NCAPG was associated with the clinical prognosis of pancreatic cancer especially with tumor size (P=0.010). Knockdown NCAPG could restrain the proliferation of renal clear cell carcinoma. CDK1 was found to be tightly related with NCAPG, and the expression of CDK1 was also associated with the prognosis. Conclusions. NCAPG was upregulated in renal clear cell carcinoma, which was related with tumor size and overall survival. NCAPG might promote the proliferation of renal clear cell carcinoma via mediating CDK1. NCAPG/CDK1 complex might provide a new treatment strategy for lots of patients with renal clear cell carcinoma.

目的 探讨3D打印引导聚醚醚酮(PEEK)行儿童颅骨修补术的临床效果.方法 回顾性分析3例颅骨肿物病儿资料,均在3D打印技术辅助下,颅骨肿物切除后同时行PEEK材料的修补手术.结果 3例病儿均成功按预定设计顺利完成手术.病理结果:嗜酸性肉芽肿2例,颅骨海绵状血管瘤1例.术后随访6个月,病儿恢复良好,头颅外形正常,肿物无复发,修补材料无移位.结论 3D打印技术引导PEEK材料应用于儿童颅骨肿物切除后一期修补手术,效果良好,但仍需长期随访.

Background Prostate cancer (PCa) is one of the most common malignant cancer in males worldwide. Circular RNAs (CircRNAs) are novel type of non-coding RNAs. Recently, circRNAs have been reported participating in various cancers, including prostate cancer. However, the function and mechanism of circ_0057553 remain to be elucidated. Methods and Materials The RNA expression levels of circ_0057553, miR-515-5p, YES proto-oncogene 1 (YES1) and glycolytic genes mRNA were detected by qRT-PCR in PCa tissues or cells. Western blotting was performed to analyze YES1 protein level. Cell viability, migration and invasion and cell apoptosis were assessed by cell counting kit-8 (CCK-8) assay, transwell assay and flow cytometry. In addition, the effects of cell glycolysis were evaluated by measuring lactate production, glucose consumption and adenosine triphosphate (ATP) level. Moreover, dual-luciferase reporter assay was used to detect the target sites of circ_0057553 and miR-515-5p, miR-515-5p and YES1. RNA immunoprecipitation (RIP) was conducted to evaluate the target relationship between circ_0057553 and miR-515-5p. Xenograft mouse model was conducted to measure tumor formation in vivo. Results Circ_0057553 was significantly up-regulated in PCa tissues and cells. Knockdown of circ_0057553 inhibited cell viability, migration, invasion and glycolysis and facilitated apoptosis in PCa cells. Furthermore, circ_0057553 bound to miR-515-5p and miR-515-5p directly targeted YES1. Interestingly, miR-515-5p inhibitor partially rescued the function of circ_0057553 knockdown, while YES1 restored the effects of miR-515-5p overexpression. Circ_0057553 down-regulation remarkably decreased tumor volume and weight in vivo. Conclusion Circ_0057553 affected PCa cell viability, migration, invasion, apoptosis and glycolysis through miR-515-5p/YES1 axis.

Introduction Ribosome binding protein 1 (RRBP1) is reported to be correlated with tumor formation and progression. However, the role of RRBP1 in bladder cancer is unclear. In this study, we aimed to investigate the expression of RRBP1 and its influence on cell proliferation in bladder cancer. Methods Quantification real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were used to detect the expression levels of RRBP1 in 138 bladder cancer and matched adjacent normal bladder tissues. Then, the clinical significance of RRBP1 in bladder cancer was evaluated. The effect of RRBP1 on cell proliferation and its potential mechanism were further explored. Results Results show that the mRNA levels of RRBP1 in bladder cancer were significantly higher compared with those in normal tissues (P< 0.001). IHC results show the high-expression rate of RRBP1 in bladder cancer was 68.8%, which was significantly greater than those in normal tissues (40.6%, P< 0.001). RRBP1 high-expression was significantly associated with differentiation, T stage and lymph node metastasis in bladder cancer (P< 0.05). The overall survival time of patients with RRBP1 high-expression was significantly reduced compared to those with RRBP1 low-expression. Moreover, RRBP1 overexpression significantly promoted cell proliferation, which was correlated with Smad1/Smad3/TGF-β1 signal pathway. Conclusion RRBP1 high-expression correlates with prognosis and promotes cell proliferation in bladder cancer, which could be a potential biomarker.